Amino acid dipepetide frequency for Vibrio phage VAI2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.486AlaAla: 6.486 ± 2.257
1.622AlaCys: 1.622 ± 0.991
3.243AlaAsp: 3.243 ± 0.843
3.784AlaGlu: 3.784 ± 1.375
4.865AlaPhe: 4.865 ± 1.194
2.703AlaGly: 2.703 ± 1.066
1.622AlaHis: 1.622 ± 0.751
4.324AlaIle: 4.324 ± 1.339
5.405AlaLys: 5.405 ± 1.294
9.73AlaLeu: 9.73 ± 1.654
0.541AlaMet: 0.541 ± 0.553
3.784AlaAsn: 3.784 ± 1.384
2.703AlaPro: 2.703 ± 1.118
2.703AlaGln: 2.703 ± 0.722
2.703AlaArg: 2.703 ± 0.848
7.027AlaSer: 7.027 ± 1.79
2.162AlaThr: 2.162 ± 1.384
7.568AlaVal: 7.568 ± 2.939
0.0AlaTrp: 0.0 ± 0.0
3.784AlaTyr: 3.784 ± 1.172
0.0AlaXaa: 0.0 ± 0.0
Cys
0.541CysAla: 0.541 ± 0.468
0.0CysCys: 0.0 ± 0.0
1.081CysAsp: 1.081 ± 0.544
1.622CysGlu: 1.622 ± 1.04
0.541CysPhe: 0.541 ± 0.437
0.541CysGly: 0.541 ± 0.468
0.541CysHis: 0.541 ± 0.468
0.0CysIle: 0.0 ± 0.0
1.081CysLys: 1.081 ± 0.495
1.081CysLeu: 1.081 ± 0.495
0.0CysMet: 0.0 ± 0.0
0.541CysAsn: 0.541 ± 0.468
1.622CysPro: 1.622 ± 0.556
0.541CysGln: 0.541 ± 0.437
0.541CysArg: 0.541 ± 0.429
0.541CysSer: 0.541 ± 0.429
3.243CysThr: 3.243 ± 1.309
1.622CysVal: 1.622 ± 0.942
0.541CysTrp: 0.541 ± 0.437
0.541CysTyr: 0.541 ± 0.468
0.0CysXaa: 0.0 ± 0.0
Asp
5.405AspAla: 5.405 ± 1.513
1.622AspCys: 1.622 ± 0.782
4.324AspAsp: 4.324 ± 1.378
2.162AspGlu: 2.162 ± 0.899
3.243AspPhe: 3.243 ± 1.358
3.784AspGly: 3.784 ± 1.04
0.0AspHis: 0.0 ± 0.0
4.324AspIle: 4.324 ± 1.887
2.162AspLys: 2.162 ± 0.761
4.324AspLeu: 4.324 ± 2.284
2.703AspMet: 2.703 ± 1.375
3.784AspAsn: 3.784 ± 1.921
4.324AspPro: 4.324 ± 1.272
2.162AspGln: 2.162 ± 0.931
1.081AspArg: 1.081 ± 0.495
3.784AspSer: 3.784 ± 1.04
3.784AspThr: 3.784 ± 1.15
3.243AspVal: 3.243 ± 1.336
0.541AspTrp: 0.541 ± 0.437
1.622AspTyr: 1.622 ± 0.681
0.0AspXaa: 0.0 ± 0.0
Glu
2.162GluAla: 2.162 ± 1.231
1.081GluCys: 1.081 ± 0.495
1.081GluAsp: 1.081 ± 0.661
1.622GluGlu: 1.622 ± 0.83
2.703GluPhe: 2.703 ± 0.921
3.243GluGly: 3.243 ± 1.008
2.162GluHis: 2.162 ± 1.002
2.162GluIle: 2.162 ± 0.96
2.703GluLys: 2.703 ± 1.643
5.946GluLeu: 5.946 ± 1.467
0.541GluMet: 0.541 ± 0.468
0.541GluAsn: 0.541 ± 0.468
1.081GluPro: 1.081 ± 0.661
3.243GluGln: 3.243 ± 1.121
2.162GluArg: 2.162 ± 1.019
4.324GluSer: 4.324 ± 1.113
1.622GluThr: 1.622 ± 0.556
1.622GluVal: 1.622 ± 0.384
0.0GluTrp: 0.0 ± 0.0
2.703GluTyr: 2.703 ± 1.017
0.0GluXaa: 0.0 ± 0.0
Phe
3.243PheAla: 3.243 ± 1.064
1.622PheCys: 1.622 ± 0.808
2.703PheAsp: 2.703 ± 0.882
2.703PheGlu: 2.703 ± 1.072
4.324PhePhe: 4.324 ± 2.226
2.703PheGly: 2.703 ± 1.034
1.081PheHis: 1.081 ± 0.495
2.703PheIle: 2.703 ± 0.936
3.784PheLys: 3.784 ± 1.524
4.865PheLeu: 4.865 ± 1.933
1.081PheMet: 1.081 ± 0.637
1.081PheAsn: 1.081 ± 0.692
0.541PhePro: 0.541 ± 0.437
2.162PheGln: 2.162 ± 0.854
2.162PheArg: 2.162 ± 1.29
2.703PheSer: 2.703 ± 1.257
2.703PheThr: 2.703 ± 1.731
6.486PheVal: 6.486 ± 1.316
1.081PheTrp: 1.081 ± 0.495
1.622PheTyr: 1.622 ± 1.001
0.0PheXaa: 0.0 ± 0.0
Gly
4.324GlyAla: 4.324 ± 1.543
1.622GlyCys: 1.622 ± 0.891
3.784GlyAsp: 3.784 ± 1.493
1.622GlyGlu: 1.622 ± 1.23
4.324GlyPhe: 4.324 ± 1.542
7.027GlyGly: 7.027 ± 2.735
1.081GlyHis: 1.081 ± 0.772
4.324GlyIle: 4.324 ± 1.08
3.784GlyLys: 3.784 ± 1.289
9.189GlyLeu: 9.189 ± 2.226
1.081GlyMet: 1.081 ± 0.705
2.162GlyAsn: 2.162 ± 0.711
1.081GlyPro: 1.081 ± 0.495
4.324GlyGln: 4.324 ± 1.965
6.486GlyArg: 6.486 ± 2.296
5.405GlySer: 5.405 ± 2.28
3.784GlyThr: 3.784 ± 1.108
3.243GlyVal: 3.243 ± 1.581
0.0GlyTrp: 0.0 ± 0.0
3.243GlyTyr: 3.243 ± 1.382
0.0GlyXaa: 0.0 ± 0.0
His
2.162HisAla: 2.162 ± 0.913
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
0.0HisGlu: 0.0 ± 0.0
1.081HisPhe: 1.081 ± 0.752
1.622HisGly: 1.622 ± 1.403
0.0HisHis: 0.0 ± 0.0
2.162HisIle: 2.162 ± 0.95
1.081HisLys: 1.081 ± 0.495
1.081HisLeu: 1.081 ± 0.935
0.0HisMet: 0.0 ± 0.0
1.622HisAsn: 1.622 ± 1.303
0.0HisPro: 0.0 ± 0.0
0.0HisGln: 0.0 ± 0.0
0.541HisArg: 0.541 ± 0.437
2.703HisSer: 2.703 ± 1.226
1.081HisThr: 1.081 ± 0.495
2.162HisVal: 2.162 ± 1.044
0.541HisTrp: 0.541 ± 0.468
1.081HisTyr: 1.081 ± 0.475
0.0HisXaa: 0.0 ± 0.0
Ile
4.865IleAla: 4.865 ± 1.031
1.081IleCys: 1.081 ± 0.661
8.108IleAsp: 8.108 ± 2.222
4.324IleGlu: 4.324 ± 1.466
4.324IlePhe: 4.324 ± 1.666
3.784IleGly: 3.784 ± 1.395
0.541IleHis: 0.541 ± 0.662
5.405IleIle: 5.405 ± 2.62
2.703IleLys: 2.703 ± 1.1
6.486IleLeu: 6.486 ± 2.109
0.541IleMet: 0.541 ± 0.468
4.324IleAsn: 4.324 ± 2.008
3.243IlePro: 3.243 ± 1.363
2.703IleGln: 2.703 ± 1.257
2.703IleArg: 2.703 ± 1.255
3.784IleSer: 3.784 ± 1.478
2.703IleThr: 2.703 ± 1.445
2.703IleVal: 2.703 ± 1.13
0.541IleTrp: 0.541 ± 0.468
2.162IleTyr: 2.162 ± 0.989
0.0IleXaa: 0.0 ± 0.0
Lys
3.784LysAla: 3.784 ± 0.729
0.0LysCys: 0.0 ± 0.0
2.703LysAsp: 2.703 ± 1.182
2.703LysGlu: 2.703 ± 1.204
2.162LysPhe: 2.162 ± 0.952
4.324LysGly: 4.324 ± 0.953
3.243LysHis: 3.243 ± 1.753
2.703LysIle: 2.703 ± 1.793
5.946LysLys: 5.946 ± 1.645
3.784LysLeu: 3.784 ± 1.101
2.703LysMet: 2.703 ± 1.5
3.243LysAsn: 3.243 ± 1.424
2.703LysPro: 2.703 ± 0.677
2.703LysGln: 2.703 ± 1.382
2.703LysArg: 2.703 ± 1.881
3.784LysSer: 3.784 ± 0.742
2.162LysThr: 2.162 ± 0.99
3.243LysVal: 3.243 ± 1.174
1.081LysTrp: 1.081 ± 0.684
1.622LysTyr: 1.622 ± 0.808
0.0LysXaa: 0.0 ± 0.0
Leu
9.189LeuAla: 9.189 ± 2.608
1.081LeuCys: 1.081 ± 1.324
9.73LeuAsp: 9.73 ± 2.45
2.703LeuGlu: 2.703 ± 1.095
3.784LeuPhe: 3.784 ± 1.403
7.027LeuGly: 7.027 ± 2.03
2.703LeuHis: 2.703 ± 1.382
7.027LeuIle: 7.027 ± 1.828
7.027LeuLys: 7.027 ± 1.841
9.73LeuLeu: 9.73 ± 1.702
1.081LeuMet: 1.081 ± 0.817
7.568LeuAsn: 7.568 ± 1.994
4.324LeuPro: 4.324 ± 0.889
2.703LeuGln: 2.703 ± 1.471
3.243LeuArg: 3.243 ± 0.78
6.486LeuSer: 6.486 ± 1.258
4.865LeuThr: 4.865 ± 1.778
4.324LeuVal: 4.324 ± 1.243
0.541LeuTrp: 0.541 ± 0.437
3.784LeuTyr: 3.784 ± 1.02
0.0LeuXaa: 0.0 ± 0.0
Met
3.243MetAla: 3.243 ± 0.832
0.541MetCys: 0.541 ± 0.47
1.081MetAsp: 1.081 ± 0.475
3.243MetGlu: 3.243 ± 1.874
0.541MetPhe: 0.541 ± 0.468
0.0MetGly: 0.0 ± 0.0
0.541MetHis: 0.541 ± 0.468
0.541MetIle: 0.541 ± 0.553
2.162MetLys: 2.162 ± 1.671
2.162MetLeu: 2.162 ± 1.038
0.541MetMet: 0.541 ± 0.468
1.081MetAsn: 1.081 ± 0.475
1.081MetPro: 1.081 ± 0.52
0.541MetGln: 0.541 ± 0.437
1.081MetArg: 1.081 ± 0.986
1.622MetSer: 1.622 ± 1.031
1.622MetThr: 1.622 ± 0.384
0.541MetVal: 0.541 ± 0.468
0.0MetTrp: 0.0 ± 0.0
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
3.243AsnAla: 3.243 ± 1.568
0.541AsnCys: 0.541 ± 0.698
1.622AsnAsp: 1.622 ± 0.678
1.622AsnGlu: 1.622 ± 0.878
0.541AsnPhe: 0.541 ± 0.429
4.324AsnGly: 4.324 ± 1.901
1.622AsnHis: 1.622 ± 0.966
4.865AsnIle: 4.865 ± 1.439
2.162AsnLys: 2.162 ± 0.711
3.784AsnLeu: 3.784 ± 1.576
1.622AsnMet: 1.622 ± 0.403
3.243AsnAsn: 3.243 ± 2.572
1.622AsnPro: 1.622 ± 0.556
0.541AsnGln: 0.541 ± 0.429
2.162AsnArg: 2.162 ± 1.498
7.568AsnSer: 7.568 ± 3.419
3.784AsnThr: 3.784 ± 1.53
2.162AsnVal: 2.162 ± 0.925
0.541AsnTrp: 0.541 ± 0.437
2.162AsnTyr: 2.162 ± 1.163
0.0AsnXaa: 0.0 ± 0.0
Pro
3.243ProAla: 3.243 ± 1.396
0.0ProCys: 0.0 ± 0.0
4.865ProAsp: 4.865 ± 1.304
2.703ProGlu: 2.703 ± 1.034
1.622ProPhe: 1.622 ± 0.659
0.541ProGly: 0.541 ± 0.429
0.541ProHis: 0.541 ± 0.429
1.081ProIle: 1.081 ± 0.52
2.162ProLys: 2.162 ± 1.201
4.324ProLeu: 4.324 ± 1.355
0.0ProMet: 0.0 ± 0.0
3.243ProAsn: 3.243 ± 1.007
0.541ProPro: 0.541 ± 0.655
1.622ProGln: 1.622 ± 0.851
1.622ProArg: 1.622 ± 0.83
5.946ProSer: 5.946 ± 1.903
0.541ProThr: 0.541 ± 0.429
2.703ProVal: 2.703 ± 0.995
1.081ProTrp: 1.081 ± 0.661
1.081ProTyr: 1.081 ± 0.495
0.0ProXaa: 0.0 ± 0.0
Gln
2.703GlnAla: 2.703 ± 0.863
0.541GlnCys: 0.541 ± 0.437
1.081GlnAsp: 1.081 ± 0.874
0.0GlnGlu: 0.0 ± 0.0
1.081GlnPhe: 1.081 ± 0.708
1.081GlnGly: 1.081 ± 0.475
0.541GlnHis: 0.541 ± 0.437
3.243GlnIle: 3.243 ± 0.872
1.081GlnLys: 1.081 ± 0.495
4.865GlnLeu: 4.865 ± 1.818
0.0GlnMet: 0.0 ± 0.0
1.081GlnAsn: 1.081 ± 0.475
0.541GlnPro: 0.541 ± 0.437
2.162GlnGln: 2.162 ± 0.914
2.162GlnArg: 2.162 ± 2.005
3.784GlnSer: 3.784 ± 1.123
3.784GlnThr: 3.784 ± 1.533
1.622GlnVal: 1.622 ± 1.403
0.541GlnTrp: 0.541 ± 0.468
3.243GlnTyr: 3.243 ± 1.681
0.0GlnXaa: 0.0 ± 0.0
Arg
3.784ArgAla: 3.784 ± 2.051
0.0ArgCys: 0.0 ± 0.0
1.622ArgAsp: 1.622 ± 0.978
2.162ArgGlu: 2.162 ± 1.2
1.622ArgPhe: 1.622 ± 1.5
4.324ArgGly: 4.324 ± 1.264
1.622ArgHis: 1.622 ± 0.659
4.865ArgIle: 4.865 ± 1.365
3.243ArgLys: 3.243 ± 0.762
5.405ArgLeu: 5.405 ± 1.291
2.703ArgMet: 2.703 ± 1.216
0.541ArgAsn: 0.541 ± 0.47
1.081ArgPro: 1.081 ± 0.708
1.081ArgGln: 1.081 ± 0.776
1.081ArgArg: 1.081 ± 0.661
2.703ArgSer: 2.703 ± 0.601
2.162ArgThr: 2.162 ± 0.989
1.622ArgVal: 1.622 ± 1.015
0.541ArgTrp: 0.541 ± 0.468
2.162ArgTyr: 2.162 ± 1.019
0.0ArgXaa: 0.0 ± 0.0
Ser
5.946SerAla: 5.946 ± 1.398
0.541SerCys: 0.541 ± 0.468
4.324SerAsp: 4.324 ± 1.914
4.324SerGlu: 4.324 ± 1.07
3.784SerPhe: 3.784 ± 0.837
9.189SerGly: 9.189 ± 2.114
0.0SerHis: 0.0 ± 0.0
4.865SerIle: 4.865 ± 1.442
1.622SerLys: 1.622 ± 0.83
8.649SerLeu: 8.649 ± 2.455
1.622SerMet: 1.622 ± 0.723
5.405SerAsn: 5.405 ± 1.547
4.324SerPro: 4.324 ± 1.202
1.081SerGln: 1.081 ± 0.857
5.405SerArg: 5.405 ± 0.957
10.27SerSer: 10.27 ± 3.689
5.405SerThr: 5.405 ± 1.941
5.405SerVal: 5.405 ± 0.897
0.541SerTrp: 0.541 ± 0.468
2.703SerTyr: 2.703 ± 1.235
0.0SerXaa: 0.0 ± 0.0
Thr
3.784ThrAla: 3.784 ± 1.334
2.703ThrCys: 2.703 ± 1.622
2.162ThrAsp: 2.162 ± 0.949
1.622ThrGlu: 1.622 ± 0.86
1.081ThrPhe: 1.081 ± 0.692
8.649ThrGly: 8.649 ± 2.781
1.081ThrHis: 1.081 ± 0.709
3.243ThrIle: 3.243 ± 1.259
2.703ThrLys: 2.703 ± 1.266
3.784ThrLeu: 3.784 ± 0.864
2.162ThrMet: 2.162 ± 0.907
2.162ThrAsn: 2.162 ± 0.949
4.865ThrPro: 4.865 ± 1.017
3.243ThrGln: 3.243 ± 1.184
1.081ThrArg: 1.081 ± 0.708
3.784ThrSer: 3.784 ± 1.068
4.324ThrThr: 4.324 ± 1.217
2.162ThrVal: 2.162 ± 1.576
0.541ThrTrp: 0.541 ± 0.429
2.703ThrTyr: 2.703 ± 1.047
0.0ThrXaa: 0.0 ± 0.0
Val
3.243ValAla: 3.243 ± 1.324
1.081ValCys: 1.081 ± 0.544
2.162ValAsp: 2.162 ± 1.04
0.541ValGlu: 0.541 ± 0.47
5.405ValPhe: 5.405 ± 1.887
4.865ValGly: 4.865 ± 2.065
0.0ValHis: 0.0 ± 0.0
7.568ValIle: 7.568 ± 1.445
2.703ValLys: 2.703 ± 0.876
5.405ValLeu: 5.405 ± 1.58
1.622ValMet: 1.622 ± 0.678
1.622ValAsn: 1.622 ± 1.208
2.703ValPro: 2.703 ± 0.945
0.541ValGln: 0.541 ± 0.581
3.243ValArg: 3.243 ± 1.956
5.405ValSer: 5.405 ± 1.355
4.324ValThr: 4.324 ± 1.584
3.784ValVal: 3.784 ± 1.731
0.0ValTrp: 0.0 ± 0.0
1.081ValTyr: 1.081 ± 1.306
0.0ValXaa: 0.0 ± 0.0
Trp
1.622TrpAla: 1.622 ± 0.858
0.541TrpCys: 0.541 ± 0.437
0.541TrpAsp: 0.541 ± 0.437
0.0TrpGlu: 0.0 ± 0.0
0.541TrpPhe: 0.541 ± 0.468
0.541TrpGly: 0.541 ± 0.437
0.0TrpHis: 0.0 ± 0.0
0.0TrpIle: 0.0 ± 0.0
0.0TrpLys: 0.0 ± 0.0
0.541TrpLeu: 0.541 ± 0.468
0.541TrpMet: 0.541 ± 0.581
0.541TrpAsn: 0.541 ± 0.468
0.541TrpPro: 0.541 ± 0.429
1.081TrpGln: 1.081 ± 0.619
1.081TrpArg: 1.081 ± 0.935
0.0TrpSer: 0.0 ± 0.0
1.081TrpThr: 1.081 ± 0.52
0.0TrpVal: 0.0 ± 0.0
0.541TrpTrp: 0.541 ± 0.468
0.0TrpTyr: 0.0 ± 0.0
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.784TyrAla: 3.784 ± 0.82
0.541TyrCys: 0.541 ± 0.429
2.162TyrAsp: 2.162 ± 1.4
3.243TyrGlu: 3.243 ± 0.903
3.784TyrPhe: 3.784 ± 1.101
1.622TyrGly: 1.622 ± 0.745
0.0TyrHis: 0.0 ± 0.0
1.622TyrIle: 1.622 ± 0.678
4.324TyrLys: 4.324 ± 0.928
3.784TyrLeu: 3.784 ± 1.871
0.541TyrMet: 0.541 ± 0.429
2.162TyrAsn: 2.162 ± 0.632
0.541TyrPro: 0.541 ± 0.437
0.0TyrGln: 0.0 ± 0.0
1.081TyrArg: 1.081 ± 0.495
3.784TyrSer: 3.784 ± 0.863
2.703TyrThr: 2.703 ± 1.376
1.081TyrVal: 1.081 ± 0.746
0.541TyrTrp: 0.541 ± 0.468
1.622TyrTyr: 1.622 ± 1.001
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 11 proteins (1851 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski