Tinamus guttatus (White-throated tinamou)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099ZKJ5|A0A099ZKJ5_TINGU Protein MAK16 (Fragment) OS=Tinamus guttatus OX=94827 GN=N309_12231 PE=4 SV=1
SS1 pKa = 6.83RR2 pKa = 11.84LSWLDD7 pKa = 3.18NSGLGFSLDD16 pKa = 3.61YY17 pKa = 9.22PTISLHH23 pKa = 6.36AVSRR27 pKa = 11.84DD28 pKa = 3.07LNAYY32 pKa = 7.9PWEE35 pKa = 4.19HH36 pKa = 6.86LYY38 pKa = 11.74VMVNAKK44 pKa = 10.37FEE46 pKa = 4.14EE47 pKa = 4.88DD48 pKa = 3.52NAKK51 pKa = 9.13EE52 pKa = 3.9APMAEE57 pKa = 4.15GEE59 pKa = 4.26EE60 pKa = 4.27EE61 pKa = 4.29EE62 pKa = 4.35EE63 pKa = 5.91AEE65 pKa = 5.56DD66 pKa = 5.18SDD68 pKa = 6.46DD69 pKa = 3.65DD70 pKa = 4.13TEE72 pKa = 5.23PIAEE76 pKa = 4.12FRR78 pKa = 11.84FVPSDD83 pKa = 3.36KK84 pKa = 10.88SALEE88 pKa = 3.9AMFSAMCEE96 pKa = 4.27CQALHH101 pKa = 7.39PDD103 pKa = 4.25PEE105 pKa = 6.28DD106 pKa = 3.73EE107 pKa = 6.39DD108 pKa = 4.23SDD110 pKa = 3.72NDD112 pKa = 4.0YY113 pKa = 11.37EE114 pKa = 4.59GEE116 pKa = 4.16EE117 pKa = 4.41YY118 pKa = 10.56DD119 pKa = 4.87VEE121 pKa = 4.22AHH123 pKa = 6.63EE124 pKa = 5.44LGQGDD129 pKa = 3.65IPSFYY134 pKa = 9.93TYY136 pKa = 11.03EE137 pKa = 4.17EE138 pKa = 4.58GLSHH142 pKa = 6.9LTAEE146 pKa = 4.71GQATLEE152 pKa = 4.11RR153 pKa = 11.84LEE155 pKa = 4.74GMLAQSVSSQYY166 pKa = 11.66NMAGVRR172 pKa = 11.84TEE174 pKa = 3.7DD175 pKa = 3.58SIRR178 pKa = 11.84EE179 pKa = 4.1FEE181 pKa = 5.41DD182 pKa = 3.49GMEE185 pKa = 4.06VDD187 pKa = 5.01LAPAVAGQFEE197 pKa = 4.53DD198 pKa = 5.38AEE200 pKa = 4.32VDD202 pKa = 3.59HH203 pKa = 7.08

Molecular weight: 22.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099Z308|A0A099Z308_TINGU Eukaryotic translation initiation factor 3 subunit D (Fragment) OS=Tinamus guttatus OX=94827 GN=N309_04024 PE=3 SV=1
TT1 pKa = 6.86QATQRR6 pKa = 11.84TQATQRR12 pKa = 11.84TQATQRR18 pKa = 11.84TQATQRR24 pKa = 11.84TQATQRR30 pKa = 11.84TQATQRR36 pKa = 11.84TQATQRR42 pKa = 11.84TQATQLTQATQRR54 pKa = 11.84TQATQLTQATQRR66 pKa = 11.84TQATQRR72 pKa = 11.84TQATQRR78 pKa = 11.84TQATQRR84 pKa = 11.84TQATQRR90 pKa = 11.84TQATQRR96 pKa = 11.84TT97 pKa = 4.22

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13377

0

13377

5354997

33

8744

400.3

45.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.55 ± 0.019

2.258 ± 0.017

5.019 ± 0.014

7.209 ± 0.029

3.955 ± 0.015

5.844 ± 0.026

2.573 ± 0.01

4.98 ± 0.015

6.481 ± 0.027

9.816 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.009

4.102 ± 0.014

5.204 ± 0.024

4.573 ± 0.018

5.349 ± 0.018

8.062 ± 0.03

5.274 ± 0.016

6.318 ± 0.018

1.2 ± 0.007

2.999 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski