Amino acid dipepetide frequency for Pseudohongiella spirulinae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.968AlaAla: 10.968 ± 0.158	1.085AlaCys: 1.085 ± 0.031	6.171AlaAsp: 6.171 ± 0.086	6.329AlaGlu: 6.329 ± 0.092	3.449AlaPhe: 3.449 ± 0.063	8.234AlaGly: 8.234 ± 0.115	1.824AlaHis: 1.824 ± 0.045	5.415AlaIle: 5.415 ± 0.081	2.37AlaLys: 2.37 ± 0.064	11.233AlaLeu: 11.233 ± 0.135	2.908AlaMet: 2.908 ± 0.047	3.1AlaAsn: 3.1 ± 0.054	3.726AlaPro: 3.726 ± 0.068	4.347AlaGln: 4.347 ± 0.062	6.216AlaArg: 6.216 ± 0.083	5.87AlaSer: 5.87 ± 0.08	4.579AlaThr: 4.579 ± 0.077	7.068AlaVal: 7.068 ± 0.092	1.283AlaTrp: 1.283 ± 0.035	2.377AlaTyr: 2.377 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.881CysAla: 0.881 ± 0.033	0.129CysCys: 0.129 ± 0.011	0.543CysAsp: 0.543 ± 0.025	0.514CysGlu: 0.514 ± 0.026	0.386CysPhe: 0.386 ± 0.018	0.816CysGly: 0.816 ± 0.033	0.331CysHis: 0.331 ± 0.016	0.466CysIle: 0.466 ± 0.021	0.26CysLys: 0.26 ± 0.017	1.056CysLeu: 1.056 ± 0.039	0.194CysMet: 0.194 ± 0.012	0.246CysAsn: 0.246 ± 0.015	0.448CysPro: 0.448 ± 0.023	0.47CysGln: 0.47 ± 0.021	0.636CysArg: 0.636 ± 0.026	0.589CysSer: 0.589 ± 0.027	0.428CysThr: 0.428 ± 0.023	0.577CysVal: 0.577 ± 0.025	0.114CysTrp: 0.114 ± 0.012	0.274CysTyr: 0.274 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	5.485AspAla: 5.485 ± 0.082	0.56AspCys: 0.56 ± 0.022	3.414AspAsp: 3.414 ± 0.068	3.786AspGlu: 3.786 ± 0.07	2.48AspPhe: 2.48 ± 0.051	4.425AspGly: 4.425 ± 0.063	1.242AspHis: 1.242 ± 0.045	3.815AspIle: 3.815 ± 0.07	2.067AspLys: 2.067 ± 0.056	5.651AspLeu: 5.651 ± 0.079	1.59AspMet: 1.59 ± 0.043	2.306AspAsn: 2.306 ± 0.051	2.824AspPro: 2.824 ± 0.06	2.558AspGln: 2.558 ± 0.048	3.382AspArg: 3.382 ± 0.056	3.577AspSer: 3.577 ± 0.065	2.909AspThr: 2.909 ± 0.058	4.15AspVal: 4.15 ± 0.071	0.964AspTrp: 0.964 ± 0.031	1.951AspTyr: 1.951 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.209GluAla: 5.209 ± 0.08	0.401GluCys: 0.401 ± 0.02	2.711GluAsp: 2.711 ± 0.051	3.34GluGlu: 3.34 ± 0.062	2.339GluPhe: 2.339 ± 0.049	3.45GluGly: 3.45 ± 0.055	1.483GluHis: 1.483 ± 0.038	3.505GluIle: 3.505 ± 0.061	2.219GluLys: 2.219 ± 0.056	6.98GluLeu: 6.98 ± 0.103	1.463GluMet: 1.463 ± 0.035	2.108GluAsn: 2.108 ± 0.046	2.535GluPro: 2.535 ± 0.077	3.879GluGln: 3.879 ± 0.079	4.196GluArg: 4.196 ± 0.073	3.596GluSer: 3.596 ± 0.056	3.038GluThr: 3.038 ± 0.06	3.806GluVal: 3.806 ± 0.065	0.716GluTrp: 0.716 ± 0.028	1.447GluTyr: 1.447 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	3.573PheAla: 3.573 ± 0.063	0.388PheCys: 0.388 ± 0.018	2.652PheAsp: 2.652 ± 0.056	2.239PheGlu: 2.239 ± 0.047	1.494PhePhe: 1.494 ± 0.046	3.098PheGly: 3.098 ± 0.065	0.738PheHis: 0.738 ± 0.026	2.032PheIle: 2.032 ± 0.05	1.059PheLys: 1.059 ± 0.032	3.353PheLeu: 3.353 ± 0.068	1.027PheMet: 1.027 ± 0.036	1.575PheAsn: 1.575 ± 0.046	1.496PhePro: 1.496 ± 0.043	1.243PheGln: 1.243 ± 0.033	2.249PheArg: 2.249 ± 0.047	2.997PheSer: 2.997 ± 0.054	2.039PheThr: 2.039 ± 0.048	2.758PheVal: 2.758 ± 0.069	0.533PheTrp: 0.533 ± 0.024	1.171PheTyr: 1.171 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	6.364GlyAla: 6.364 ± 0.085	0.741GlyCys: 0.741 ± 0.029	4.161GlyAsp: 4.161 ± 0.07	4.381GlyGlu: 4.381 ± 0.07	3.277GlyPhe: 3.277 ± 0.06	5.809GlyGly: 5.809 ± 0.135	1.681GlyHis: 1.681 ± 0.045	4.591GlyIle: 4.591 ± 0.071	2.837GlyLys: 2.837 ± 0.058	7.939GlyLeu: 7.939 ± 0.091	2.198GlyMet: 2.198 ± 0.049	2.757GlyAsn: 2.757 ± 0.067	2.402GlyPro: 2.402 ± 0.055	3.414GlyGln: 3.414 ± 0.055	4.677GlyArg: 4.677 ± 0.072	4.855GlySer: 4.855 ± 0.084	3.875GlyThr: 3.875 ± 0.09	5.448GlyVal: 5.448 ± 0.08	1.174GlyTrp: 1.174 ± 0.041	2.25GlyTyr: 2.25 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.834HisAla: 1.834 ± 0.047	0.268HisCys: 0.268 ± 0.018	1.214HisAsp: 1.214 ± 0.048	1.179HisGlu: 1.179 ± 0.03	0.933HisPhe: 0.933 ± 0.035	1.671HisGly: 1.671 ± 0.047	0.644HisHis: 0.644 ± 0.028	1.21HisIle: 1.21 ± 0.034	0.756HisLys: 0.756 ± 0.029	2.12HisLeu: 2.12 ± 0.046	0.487HisMet: 0.487 ± 0.02	0.796HisAsn: 0.796 ± 0.031	1.311HisPro: 1.311 ± 0.041	1.001HisGln: 1.001 ± 0.032	1.362HisArg: 1.362 ± 0.037	1.322HisSer: 1.322 ± 0.036	1.122HisThr: 1.122 ± 0.037	1.344HisVal: 1.344 ± 0.044	0.377HisTrp: 0.377 ± 0.018	0.795HisTyr: 0.795 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	5.939IleAla: 5.939 ± 0.079	0.638IleCys: 0.638 ± 0.024	3.976IleAsp: 3.976 ± 0.064	3.673IleGlu: 3.673 ± 0.061	1.796IlePhe: 1.796 ± 0.047	4.247IleGly: 4.247 ± 0.067	1.154IleHis: 1.154 ± 0.036	2.962IleIle: 2.962 ± 0.067	2.072IleLys: 2.072 ± 0.054	4.799IleLeu: 4.799 ± 0.08	1.307IleMet: 1.307 ± 0.045	2.642IleAsn: 2.642 ± 0.054	2.63IlePro: 2.63 ± 0.05	1.837IleGln: 1.837 ± 0.043	3.693IleArg: 3.693 ± 0.061	4.127IleSer: 4.127 ± 0.071	3.143IleThr: 3.143 ± 0.059	3.968IleVal: 3.968 ± 0.069	0.603IleTrp: 0.603 ± 0.026	1.459IleTyr: 1.459 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	3.206LysAla: 3.206 ± 0.076	0.205LysCys: 0.205 ± 0.016	1.705LysAsp: 1.705 ± 0.042	1.663LysGlu: 1.663 ± 0.054	0.881LysPhe: 0.881 ± 0.031	2.027LysGly: 2.027 ± 0.044	0.707LysHis: 0.707 ± 0.027	1.766LysIle: 1.766 ± 0.051	1.446LysLys: 1.446 ± 0.06	3.264LysLeu: 3.264 ± 0.063	0.768LysMet: 0.768 ± 0.026	1.164LysAsn: 1.164 ± 0.032	1.606LysPro: 1.606 ± 0.046	1.533LysGln: 1.533 ± 0.039	1.991LysArg: 1.991 ± 0.047	1.983LysSer: 1.983 ± 0.052	1.86LysThr: 1.86 ± 0.051	2.303LysVal: 2.303 ± 0.052	0.321LysTrp: 0.321 ± 0.015	0.718LysTyr: 0.718 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	11.383LeuAla: 11.383 ± 0.139	1.038LeuCys: 1.038 ± 0.033	6.12LeuAsp: 6.12 ± 0.092	5.689LeuGlu: 5.689 ± 0.099	3.733LeuPhe: 3.733 ± 0.068	7.415LeuGly: 7.415 ± 0.085	2.196LeuHis: 2.196 ± 0.057	6.091LeuIle: 6.091 ± 0.091	3.528LeuLys: 3.528 ± 0.066	11.514LeuLeu: 11.514 ± 0.161	2.817LeuMet: 2.817 ± 0.058	3.989LeuAsn: 3.989 ± 0.07	5.347LeuPro: 5.347 ± 0.069	5.227LeuGln: 5.227 ± 0.091	6.541LeuArg: 6.541 ± 0.088	7.682LeuSer: 7.682 ± 0.096	5.819LeuThr: 5.819 ± 0.072	7.074LeuVal: 7.074 ± 0.11	1.187LeuTrp: 1.187 ± 0.035	2.603LeuTyr: 2.603 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.81MetAla: 2.81 ± 0.041	0.184MetCys: 0.184 ± 0.015	1.272MetAsp: 1.272 ± 0.038	1.241MetGlu: 1.241 ± 0.037	0.845MetPhe: 0.845 ± 0.03	1.75MetGly: 1.75 ± 0.052	0.533MetHis: 0.533 ± 0.023	1.538MetIle: 1.538 ± 0.046	0.946MetLys: 0.946 ± 0.029	2.933MetLeu: 2.933 ± 0.061	0.721MetMet: 0.721 ± 0.029	1.037MetAsn: 1.037 ± 0.031	1.407MetPro: 1.407 ± 0.036	1.361MetGln: 1.361 ± 0.037	1.627MetArg: 1.627 ± 0.038	2.038MetSer: 2.038 ± 0.039	1.73MetThr: 1.73 ± 0.045	1.599MetVal: 1.599 ± 0.038	0.212MetTrp: 0.212 ± 0.014	0.482MetTyr: 0.482 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	3.316AsnAla: 3.316 ± 0.061	0.352AsnCys: 0.352 ± 0.018	1.964AsnAsp: 1.964 ± 0.047	1.898AsnGlu: 1.898 ± 0.044	1.29AsnPhe: 1.29 ± 0.042	2.75AsnGly: 2.75 ± 0.065	0.731AsnHis: 0.731 ± 0.03	2.177AsnIle: 2.177 ± 0.045	1.12AsnLys: 1.12 ± 0.036	3.55AsnLeu: 3.55 ± 0.069	0.947AsnMet: 0.947 ± 0.032	1.531AsnAsn: 1.531 ± 0.043	2.218AsnPro: 2.218 ± 0.052	1.575AsnGln: 1.575 ± 0.042	2.281AsnArg: 2.281 ± 0.05	2.135AsnSer: 2.135 ± 0.054	2.054AsnThr: 2.054 ± 0.044	2.276AsnVal: 2.276 ± 0.046	0.595AsnTrp: 0.595 ± 0.023	1.129AsnTyr: 1.129 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	4.938ProAla: 4.938 ± 0.069	0.327ProCys: 0.327 ± 0.019	3.774ProAsp: 3.774 ± 0.068	3.626ProGlu: 3.626 ± 0.089	1.668ProPhe: 1.668 ± 0.042	4.094ProGly: 4.094 ± 0.071	0.879ProHis: 0.879 ± 0.032	1.969ProIle: 1.969 ± 0.044	0.977ProLys: 0.977 ± 0.032	4.477ProLeu: 4.477 ± 0.066	1.171ProMet: 1.171 ± 0.03	1.254ProAsn: 1.254 ± 0.031	1.899ProPro: 1.899 ± 0.055	1.916ProGln: 1.916 ± 0.051	2.187ProArg: 2.187 ± 0.049	2.445ProSer: 2.445 ± 0.046	2.014ProThr: 2.014 ± 0.054	4.064ProVal: 4.064 ± 0.068	0.658ProTrp: 0.658 ± 0.024	1.121ProTyr: 1.121 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	5.183GlnAla: 5.183 ± 0.074	0.387GlnCys: 0.387 ± 0.02	2.246GlnAsp: 2.246 ± 0.047	2.283GlnGlu: 2.283 ± 0.057	1.711GlnPhe: 1.711 ± 0.046	3.123GlnGly: 3.123 ± 0.055	1.11GlnHis: 1.11 ± 0.032	2.655GlnIle: 2.655 ± 0.046	1.355GlnLys: 1.355 ± 0.043	5.231GlnLeu: 5.231 ± 0.083	1.23GlnMet: 1.23 ± 0.039	1.425GlnAsn: 1.425 ± 0.037	2.099GlnPro: 2.099 ± 0.045	3.382GlnGln: 3.382 ± 0.095	3.399GlnArg: 3.399 ± 0.069	3.124GlnSer: 3.124 ± 0.063	2.379GlnThr: 2.379 ± 0.054	3.225GlnVal: 3.225 ± 0.059	0.738GlnTrp: 0.738 ± 0.029	1.143GlnTyr: 1.143 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	5.315ArgAla: 5.315 ± 0.078	0.535ArgCys: 0.535 ± 0.025	3.723ArgAsp: 3.723 ± 0.068	3.952ArgGlu: 3.952 ± 0.069	2.701ArgPhe: 2.701 ± 0.051	3.941ArgGly: 3.941 ± 0.063	1.688ArgHis: 1.688 ± 0.041	3.879ArgIle: 3.879 ± 0.058	2.04ArgLys: 2.04 ± 0.043	7.267ArgLeu: 7.267 ± 0.099	1.679ArgMet: 1.679 ± 0.045	2.149ArgAsn: 2.149 ± 0.048	2.683ArgPro: 2.683 ± 0.048	3.669ArgGln: 3.669 ± 0.068	4.194ArgArg: 4.194 ± 0.078	3.702ArgSer: 3.702 ± 0.061	2.86ArgThr: 2.86 ± 0.053	4.197ArgVal: 4.197 ± 0.063	0.939ArgTrp: 0.939 ± 0.028	2.119ArgTyr: 2.119 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	6.702SerAla: 6.702 ± 0.086	0.541SerCys: 0.541 ± 0.024	4.006SerAsp: 4.006 ± 0.067	3.639SerGlu: 3.639 ± 0.066	2.372SerPhe: 2.372 ± 0.057	5.813SerGly: 5.813 ± 0.087	1.348SerHis: 1.348 ± 0.039	3.48SerIle: 3.48 ± 0.057	1.777SerLys: 1.777 ± 0.049	6.943SerLeu: 6.943 ± 0.085	1.65SerMet: 1.65 ± 0.041	2.18SerAsn: 2.18 ± 0.053	2.814SerPro: 2.814 ± 0.049	2.786SerGln: 2.786 ± 0.055	4.192SerArg: 4.192 ± 0.059	4.053SerSer: 4.053 ± 0.078	3.098SerThr: 3.098 ± 0.073	4.647SerVal: 4.647 ± 0.071	0.856SerTrp: 0.856 ± 0.033	1.672SerTyr: 1.672 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	5.217ThrAla: 5.217 ± 0.077	0.38ThrCys: 0.38 ± 0.023	3.051ThrAsp: 3.051 ± 0.057	3.033ThrGlu: 3.033 ± 0.054	1.783ThrPhe: 1.783 ± 0.049	4.774ThrGly: 4.774 ± 0.087	1.085ThrHis: 1.085 ± 0.037	2.761ThrIle: 2.761 ± 0.057	0.993ThrLys: 0.993 ± 0.036	6.287ThrLeu: 6.287 ± 0.077	1.098ThrMet: 1.098 ± 0.034	1.512ThrAsn: 1.512 ± 0.048	2.893ThrPro: 2.893 ± 0.061	2.049ThrGln: 2.049 ± 0.046	3.181ThrArg: 3.181 ± 0.055	2.97ThrSer: 2.97 ± 0.059	2.641ThrThr: 2.641 ± 0.066	4.067ThrVal: 4.067 ± 0.069	0.617ThrTrp: 0.617 ± 0.024	1.313ThrTyr: 1.313 ± 0.038	0.0ThrXaa: 0.0 ± 0.0
Val	6.844ValAla: 6.844 ± 0.103	0.764ValCys: 0.764 ± 0.03	4.189ValAsp: 4.189 ± 0.061	3.851ValGlu: 3.851 ± 0.064	2.824ValPhe: 2.824 ± 0.056	4.488ValGly: 4.488 ± 0.074	1.385ValHis: 1.385 ± 0.039	4.35ValIle: 4.35 ± 0.072	2.235ValLys: 2.235 ± 0.051	7.625ValLeu: 7.625 ± 0.107	2.023ValMet: 2.023 ± 0.054	2.708ValAsn: 2.708 ± 0.055	3.203ValPro: 3.203 ± 0.061	2.653ValGln: 2.653 ± 0.054	4.13ValArg: 4.13 ± 0.063	5.027ValSer: 5.027 ± 0.072	4.134ValThr: 4.134 ± 0.068	5.215ValVal: 5.215 ± 0.098	0.851ValTrp: 0.851 ± 0.032	1.892ValTyr: 1.892 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.953TrpAla: 0.953 ± 0.031	0.121TrpCys: 0.121 ± 0.011	0.654TrpAsp: 0.654 ± 0.026	0.559TrpGlu: 0.559 ± 0.026	0.547TrpPhe: 0.547 ± 0.025	0.804TrpGly: 0.804 ± 0.031	0.377TrpHis: 0.377 ± 0.02	0.697TrpIle: 0.697 ± 0.027	0.316TrpLys: 0.316 ± 0.016	1.833TrpLeu: 1.833 ± 0.05	0.398TrpMet: 0.398 ± 0.02	0.457TrpAsn: 0.457 ± 0.024	0.631TrpPro: 0.631 ± 0.028	1.058TrpGln: 1.058 ± 0.037	1.025TrpArg: 1.025 ± 0.034	0.83TrpSer: 0.83 ± 0.033	0.61TrpThr: 0.61 ± 0.025	0.9TrpVal: 0.9 ± 0.031	0.199TrpTrp: 0.199 ± 0.014	0.391TrpTyr: 0.391 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.434TyrAla: 2.434 ± 0.043	0.313TyrCys: 0.313 ± 0.018	1.589TyrAsp: 1.589 ± 0.039	1.546TyrGlu: 1.546 ± 0.047	1.204TyrPhe: 1.204 ± 0.038	2.106TyrGly: 2.106 ± 0.05	0.609TyrHis: 0.609 ± 0.025	1.308TyrIle: 1.308 ± 0.036	0.746TyrLys: 0.746 ± 0.027	2.939TyrLeu: 2.939 ± 0.061	0.587TyrMet: 0.587 ± 0.025	0.954TyrAsn: 0.954 ± 0.039	1.285TyrPro: 1.285 ± 0.036	1.469TyrGln: 1.469 ± 0.034	2.073TyrArg: 2.073 ± 0.046	1.646TyrSer: 1.646 ± 0.038	1.372TyrThr: 1.372 ± 0.038	1.705TyrVal: 1.705 ± 0.045	0.423TyrTrp: 0.423 ± 0.023	0.87TyrTyr: 0.87 ± 0.033	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2984 proteins (1019366 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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