Callorhinus ursinus (Northern fur seal)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Eutheria; Boreoeutheria; Laurasiatheria; Carnivora;

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35454 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q7P7A4|A0A3Q7P7A4_CALUR rho-related BTB domain-containing protein 2 isoform X1 OS=Callorhinus ursinus OX=34884 GN=RHOBTB2 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 4.22VAEE11 pKa = 4.71KK12 pKa = 9.75WCSNTPFEE20 pKa = 5.73LIATEE25 pKa = 3.96EE26 pKa = 4.32TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.39YY73 pKa = 10.64ISSCGEE79 pKa = 3.84KK80 pKa = 9.5TLHH83 pKa = 6.14EE84 pKa = 4.06VLEE87 pKa = 4.46RR88 pKa = 11.84VFKK91 pKa = 10.68SFRR94 pKa = 11.84PLLGLPDD101 pKa = 4.83ADD103 pKa = 3.93DD104 pKa = 4.59DD105 pKa = 4.62AFEE108 pKa = 4.77EE109 pKa = 4.52YY110 pKa = 10.51SADD113 pKa = 3.68VEE115 pKa = 4.38EE116 pKa = 5.09EE117 pKa = 4.2EE118 pKa = 5.29PEE120 pKa = 4.13ADD122 pKa = 3.66HH123 pKa = 6.65PQMGVSQQQ131 pKa = 3.0

Molecular weight: 14.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q7QAR5|A0A3Q7QAR5_CALUR Dynein light chain roadblock OS=Callorhinus ursinus OX=34884 GN=LOC112832641 PE=3 SV=1
MM1 pKa = 7.87PSHH4 pKa = 5.63KK5 pKa = 9.28TFRR8 pKa = 11.84IKK10 pKa = 10.59RR11 pKa = 11.84FRR13 pKa = 11.84AKK15 pKa = 9.84KK16 pKa = 9.36QKK18 pKa = 8.85QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84MKK30 pKa = 9.89TGNKK34 pKa = 8.61IRR36 pKa = 11.84YY37 pKa = 7.09NSKK40 pKa = 8.14RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.03WRR45 pKa = 11.84RR46 pKa = 11.84IKK48 pKa = 10.84LGLL51 pKa = 3.67

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20337

15117

35454

22677905

31

34376

639.6

71.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.05 ± 0.014

2.141 ± 0.009

4.796 ± 0.009

7.231 ± 0.017

3.53 ± 0.009

6.527 ± 0.016

2.592 ± 0.007

4.233 ± 0.011

5.778 ± 0.016

9.899 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.005

3.554 ± 0.009

6.448 ± 0.02

4.888 ± 0.012

5.786 ± 0.014

8.498 ± 0.016

5.257 ± 0.01

5.937 ± 0.01

1.167 ± 0.004

2.549 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski