Haloquadratum sp. J07HQX50

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Haloquadratum; unclassified Haloquadratum

Average proteome isoelectric point is 5.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U1PZW5|U1PZW5_9EURY Spermidine/putrescine-binding periplasmic protein OS=Haloquadratum sp. J07HQX50 OX=1238426 GN=J07HQX50_02174 PE=4 SV=1
MM1 pKa = 7.76TDD3 pKa = 3.61DD4 pKa = 4.65SSPSSDD10 pKa = 4.53EE11 pKa = 3.76EE12 pKa = 3.9LTYY15 pKa = 11.33AKK17 pKa = 10.54AGVDD21 pKa = 3.08IDD23 pKa = 4.05ASEE26 pKa = 4.3SATDD30 pKa = 3.84ALLQAVGEE38 pKa = 4.52TPGDD42 pKa = 3.58YY43 pKa = 10.89AGLIDD48 pKa = 5.7IGNQYY53 pKa = 10.86LAVTTDD59 pKa = 3.45GVGTKK64 pKa = 10.53LLIAEE69 pKa = 5.67AIAEE73 pKa = 4.14YY74 pKa = 9.31STIGIDD80 pKa = 3.56CVAMNVNDD88 pKa = 4.12LVAEE92 pKa = 4.65GVRR95 pKa = 11.84PIGFVDD101 pKa = 3.97YY102 pKa = 10.83LAVDD106 pKa = 3.94NPDD109 pKa = 3.4EE110 pKa = 5.85DD111 pKa = 3.52IASQIGVGLQSGADD125 pKa = 3.41RR126 pKa = 11.84ADD128 pKa = 2.91ISLIGGEE135 pKa = 4.16TAVMPEE141 pKa = 4.27VINGFDD147 pKa = 4.1LAGTCVGLAPKK158 pKa = 10.28SDD160 pKa = 3.64VLTQTAQPGDD170 pKa = 3.88IIAGFPSSGIHH181 pKa = 6.89SNGLTLARR189 pKa = 11.84KK190 pKa = 9.52AIQNQFSYY198 pKa = 10.26TDD200 pKa = 3.6QYY202 pKa = 11.6PEE204 pKa = 3.83SQYY207 pKa = 11.77NSIAEE212 pKa = 4.2ALLEE216 pKa = 3.88PTKK219 pKa = 10.43IYY221 pKa = 9.19THH223 pKa = 6.53LLEE226 pKa = 5.5PIYY229 pKa = 9.67EE230 pKa = 4.36TGVRR234 pKa = 11.84TAAHH238 pKa = 5.58ITGGGWTNIEE248 pKa = 3.67RR249 pKa = 11.84MGEE252 pKa = 4.06NTYY255 pKa = 10.42HH256 pKa = 6.41IHH258 pKa = 7.64DD259 pKa = 4.97PLSVPSIFEE268 pKa = 4.94LIQEE272 pKa = 4.62SGNIDD277 pKa = 3.65DD278 pKa = 4.55RR279 pKa = 11.84EE280 pKa = 4.15MYY282 pKa = 9.29RR283 pKa = 11.84TFNMGIGFVCAGEE296 pKa = 4.05PSVIDD301 pKa = 3.55SLVAEE306 pKa = 4.61TDD308 pKa = 3.64GEE310 pKa = 4.68VIGEE314 pKa = 4.12VDD316 pKa = 3.06VGTGVKK322 pKa = 9.26IDD324 pKa = 3.62GMVLL328 pKa = 3.2

Molecular weight: 34.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U1Q140|U1Q140_9EURY 16S rRNA aminocarboxypropyltransferase OS=Haloquadratum sp. J07HQX50 OX=1238426 GN=J07HQX50_02621 PE=3 SV=1
MM1 pKa = 7.13QPLRR5 pKa = 11.84AYY7 pKa = 9.77SWRR10 pKa = 11.84EE11 pKa = 3.24TKK13 pKa = 9.99IYY15 pKa = 10.2YY16 pKa = 10.23DD17 pKa = 3.57RR18 pKa = 11.84FLSEE22 pKa = 4.63SRR24 pKa = 11.84PHH26 pKa = 6.88AQGQLNCISPGLTRR40 pKa = 11.84LQSIMCRR47 pKa = 11.84ALPKK51 pKa = 9.66FTGIFGWSDD60 pKa = 3.04SSTLSTATRR69 pKa = 11.84NSTALRR75 pKa = 11.84TKK77 pKa = 8.99TCEE80 pKa = 4.05SRR82 pKa = 11.84PTSQRR87 pKa = 11.84TACRR91 pKa = 11.84QLGRR95 pKa = 11.84WLLKK99 pKa = 10.14HH100 pKa = 5.93SRR102 pKa = 11.84RR103 pKa = 11.84SCASKK108 pKa = 10.36KK109 pKa = 10.76GKK111 pKa = 10.34LSANSRR117 pKa = 11.84FEE119 pKa = 4.41GLLSCMTTVTFNNDD133 pKa = 2.56HH134 pKa = 6.79CALSATDD141 pKa = 3.26QRR143 pKa = 11.84VRR145 pKa = 11.84AEE147 pKa = 3.69YY148 pKa = 10.58VRR150 pKa = 11.84RR151 pKa = 11.84HH152 pKa = 6.23DD153 pKa = 3.55SDD155 pKa = 3.77VVRR158 pKa = 11.84NRR160 pKa = 11.84LEE162 pKa = 3.64QSS164 pKa = 2.95

Molecular weight: 18.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2865

0

2865

653713

24

2413

228.2

24.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.141 ± 0.052

0.905 ± 0.017

6.763 ± 0.055

7.604 ± 0.061

3.359 ± 0.031

7.46 ± 0.059

2.237 ± 0.026

5.859 ± 0.041

2.411 ± 0.028

8.587 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.938 ± 0.022

3.189 ± 0.033

4.437 ± 0.032

3.729 ± 0.029

6.131 ± 0.05

7.192 ± 0.05

7.224 ± 0.05

7.872 ± 0.047

1.064 ± 0.018

2.742 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski