Pseudomonas phage KNP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Ghunavirus; Pseudomonas virus KNP

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JRZ7|A0A1W6JRZ7_9CAUD Endonuclease I OS=Pseudomonas phage KNP OX=2783802 GN=KNP_020 PE=4 SV=1
MM1 pKa = 7.79RR2 pKa = 11.84SYY4 pKa = 11.02EE5 pKa = 4.16ATLEE9 pKa = 4.15TDD11 pKa = 4.33DD12 pKa = 4.81EE13 pKa = 4.33LAAINDD19 pKa = 3.49MLAAIGEE26 pKa = 4.66SPVSSLEE33 pKa = 3.75GDD35 pKa = 3.85PNADD39 pKa = 2.75VANARR44 pKa = 11.84RR45 pKa = 11.84ILNQVNRR52 pKa = 11.84EE53 pKa = 4.03VQSRR57 pKa = 11.84GWTFNIEE64 pKa = 3.87EE65 pKa = 4.53GAVLSPDD72 pKa = 3.5SFSGLIEE79 pKa = 4.01YY80 pKa = 10.61LSDD83 pKa = 3.37YY84 pKa = 11.19LRR86 pKa = 11.84ITTSGGTVYY95 pKa = 10.65VNRR98 pKa = 11.84GGYY101 pKa = 9.92VYY103 pKa = 10.76DD104 pKa = 4.48RR105 pKa = 11.84STKK108 pKa = 9.41TDD110 pKa = 2.9VYY112 pKa = 10.74TNDD115 pKa = 2.79ITVDD119 pKa = 4.39LIRR122 pKa = 11.84FKK124 pKa = 10.5TFSEE128 pKa = 4.23MPEE131 pKa = 4.1CFRR134 pKa = 11.84SYY136 pKa = 10.38IVAKK140 pKa = 10.26ASRR143 pKa = 11.84RR144 pKa = 11.84FNIRR148 pKa = 11.84FFGAGEE154 pKa = 4.11IEE156 pKa = 4.68GSLQEE161 pKa = 4.25QEE163 pKa = 4.11SEE165 pKa = 3.75AWQQCQEE172 pKa = 4.14YY173 pKa = 9.05EE174 pKa = 3.73LDD176 pKa = 3.55YY177 pKa = 11.63GGFNMIDD184 pKa = 3.33GDD186 pKa = 4.42SYY188 pKa = 11.67VGGIASRR195 pKa = 3.65

Molecular weight: 21.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JRZ2|A0A1W6JRZ2_9CAUD Scaffolding protein OS=Pseudomonas phage KNP OX=2783802 GN=KNP_035 PE=4 SV=1
MM1 pKa = 7.64FGRR4 pKa = 11.84NFEE7 pKa = 4.15KK8 pKa = 8.76TTRR11 pKa = 11.84TNARR15 pKa = 11.84RR16 pKa = 11.84SFEE19 pKa = 4.23EE20 pKa = 3.84IEE22 pKa = 4.09QVRR25 pKa = 11.84SKK27 pKa = 10.61KK28 pKa = 10.34GKK30 pKa = 9.2RR31 pKa = 11.84NKK33 pKa = 8.04QARR36 pKa = 11.84GGRR39 pKa = 11.84QEE41 pKa = 3.76WSEE44 pKa = 4.03EE45 pKa = 3.88

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12659

45

1392

253.2

28.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.843 ± 0.452

0.964 ± 0.172

6.367 ± 0.254

6.62 ± 0.395

3.713 ± 0.207

7.986 ± 0.322

1.935 ± 0.206

5.079 ± 0.217

5.751 ± 0.32

8.002 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.544 ± 0.171

4.084 ± 0.348

3.918 ± 0.211

4.59 ± 0.31

5.743 ± 0.206

6.249 ± 0.415

5.459 ± 0.286

6.525 ± 0.316

1.454 ± 0.146

3.176 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski