Salegentibacter mishustinae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Salegentibacter

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q9ZFK8|A0A0Q9ZFK8_9FLAO DNA-directed RNA polymerase subunit beta OS=Salegentibacter mishustinae OX=270918 GN=rpoB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.53NIFKK6 pKa = 10.35TSLAVLSLVFFSACSNDD23 pKa = 4.09DD24 pKa = 3.53YY25 pKa = 11.57DD26 pKa = 5.15IGDD29 pKa = 3.39ITTPSNLSVTAEE41 pKa = 3.92VVGQTEE47 pKa = 4.28EE48 pKa = 4.12MPNGDD53 pKa = 2.83GSGAVSFTASADD65 pKa = 3.26NAMTYY70 pKa = 10.58KK71 pKa = 10.77FIYY74 pKa = 9.96GDD76 pKa = 3.53GFEE79 pKa = 4.54EE80 pKa = 4.82VSASGEE86 pKa = 4.27TTHH89 pKa = 6.77SFNEE93 pKa = 4.17NGVNDD98 pKa = 3.7YY99 pKa = 9.27TVTVVASGTGGASSNMTTTVTVFSDD124 pKa = 4.34FSDD127 pKa = 4.4PEE129 pKa = 4.37TKK131 pKa = 10.25EE132 pKa = 3.91LLTGGSSKK140 pKa = 8.27TWYY143 pKa = 9.79VAASQPAHH151 pKa = 6.62LGVGPSSGEE160 pKa = 3.98GFTAPIYY167 pKa = 9.79YY168 pKa = 10.31AAAPFEE174 pKa = 4.25KK175 pKa = 10.44AGADD179 pKa = 3.5VSSCFYY185 pKa = 9.46TDD187 pKa = 3.64EE188 pKa = 4.28MTFSLNEE195 pKa = 3.76NDD197 pKa = 4.05NIVYY201 pKa = 10.39NYY203 pKa = 10.04NNNGLTFVNVAYY215 pKa = 9.53TGDD218 pKa = 3.51FGGDD222 pKa = 3.4GSEE225 pKa = 4.3DD226 pKa = 3.32QCLDD230 pKa = 3.49FGNTGDD236 pKa = 4.63FSASLSPSTSGLPEE250 pKa = 4.7DD251 pKa = 4.07ATTGTVINIAGGGTMSYY268 pKa = 10.9YY269 pKa = 10.62VGSSSYY275 pKa = 10.59EE276 pKa = 3.84VLNITPTTMDD286 pKa = 3.0VRR288 pKa = 11.84VIPGNDD294 pKa = 3.45PALAWYY300 pKa = 10.15LKK302 pKa = 9.38FTTSQGGEE310 pKa = 3.95EE311 pKa = 4.14EE312 pKa = 4.87EE313 pKa = 4.29EE314 pKa = 4.44FEE316 pKa = 4.76SQFNTEE322 pKa = 3.41IWSDD326 pKa = 3.78EE327 pKa = 4.03FDD329 pKa = 3.78GDD331 pKa = 5.66ALNTDD336 pKa = 2.71NWNYY340 pKa = 7.77EE341 pKa = 4.08TGNGSNGWGNNEE353 pKa = 3.45AQYY356 pKa = 9.69YY357 pKa = 9.02TDD359 pKa = 4.96AEE361 pKa = 4.77DD362 pKa = 3.59NVKK365 pKa = 10.2VEE367 pKa = 4.21NGNLVITAKK376 pKa = 10.54RR377 pKa = 11.84EE378 pKa = 4.12AEE380 pKa = 4.06SGFDD384 pKa = 3.41FTSARR389 pKa = 11.84ITTKK393 pKa = 10.92DD394 pKa = 2.85KK395 pKa = 11.51FEE397 pKa = 4.15FTFGRR402 pKa = 11.84VEE404 pKa = 4.06VRR406 pKa = 11.84AKK408 pKa = 10.65LPAGGGTWPAIWMLGAAFPEE428 pKa = 4.61EE429 pKa = 4.24SWPGVGEE436 pKa = 3.84MDD438 pKa = 3.18IMEE441 pKa = 4.59FVGNDD446 pKa = 3.34PDD448 pKa = 4.82RR449 pKa = 11.84ISSALHH455 pKa = 5.49FPGNSGGNAIVGDD468 pKa = 4.17TEE470 pKa = 4.34VSDD473 pKa = 3.91VTSEE477 pKa = 3.56FHH479 pKa = 6.9IYY481 pKa = 8.66EE482 pKa = 4.34VEE484 pKa = 3.76WTAEE488 pKa = 4.13KK489 pKa = 9.5ITFLMDD495 pKa = 3.67GVPHH499 pKa = 7.7LEE501 pKa = 3.84FDD503 pKa = 4.53NNDD506 pKa = 3.27STPFQEE512 pKa = 5.63DD513 pKa = 3.37FFIILNVAMGGTLGGNIADD532 pKa = 4.73DD533 pKa = 4.09FQEE536 pKa = 4.24SSMEE540 pKa = 3.64VDD542 pKa = 3.49YY543 pKa = 11.86VKK545 pKa = 10.74VFQEE549 pKa = 3.96

Molecular weight: 59.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q9ZA26|A0A0Q9ZA26_9FLAO Phosphoribosylpyrophosphate synthetase OS=Salegentibacter mishustinae OX=270918 GN=APR42_15290 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.2RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.04VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.34KK41 pKa = 10.36ISVSSEE47 pKa = 3.42NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3284

0

3284

1108007

38

3537

337.4

38.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.469 ± 0.04

0.653 ± 0.015

5.538 ± 0.038

7.841 ± 0.043

5.285 ± 0.036

6.461 ± 0.042

1.712 ± 0.021

7.669 ± 0.037

7.764 ± 0.057

9.371 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.026

5.919 ± 0.046

3.48 ± 0.026

3.426 ± 0.025

3.708 ± 0.03

6.364 ± 0.029

5.217 ± 0.041

5.921 ± 0.032

1.056 ± 0.015

3.937 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski