Amino acid dipepetide frequency for Lachnospiraceae bacterium C7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.62AlaAla: 4.62 ± 0.095	0.87AlaCys: 0.87 ± 0.036	3.731AlaAsp: 3.731 ± 0.068	4.108AlaGlu: 4.108 ± 0.075	2.644AlaPhe: 2.644 ± 0.06	4.547AlaGly: 4.547 ± 0.086	0.907AlaHis: 0.907 ± 0.033	5.797AlaIle: 5.797 ± 0.095	5.971AlaLys: 5.971 ± 0.08	5.655AlaLeu: 5.655 ± 0.086	2.082AlaMet: 2.082 ± 0.053	3.26AlaAsn: 3.26 ± 0.075	1.632AlaPro: 1.632 ± 0.052	1.946AlaGln: 1.946 ± 0.053	2.294AlaArg: 2.294 ± 0.056	3.892AlaSer: 3.892 ± 0.068	3.658AlaThr: 3.658 ± 0.076	4.753AlaVal: 4.753 ± 0.081	0.429AlaTrp: 0.429 ± 0.025	2.429AlaTyr: 2.429 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.779CysAla: 0.779 ± 0.038	0.213CysCys: 0.213 ± 0.017	0.837CysAsp: 0.837 ± 0.032	0.78CysGlu: 0.78 ± 0.029	0.589CysPhe: 0.589 ± 0.031	1.274CysGly: 1.274 ± 0.038	0.242CysHis: 0.242 ± 0.019	1.077CysIle: 1.077 ± 0.036	0.955CysLys: 0.955 ± 0.039	0.977CysLeu: 0.977 ± 0.035	0.368CysMet: 0.368 ± 0.021	0.682CysAsn: 0.682 ± 0.033	0.465CysPro: 0.465 ± 0.025	0.353CysGln: 0.353 ± 0.02	0.421CysArg: 0.421 ± 0.022	0.76CysSer: 0.76 ± 0.037	0.659CysThr: 0.659 ± 0.027	0.901CysVal: 0.901 ± 0.038	0.087CysTrp: 0.087 ± 0.01	0.55CysTyr: 0.55 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.816AspAla: 3.816 ± 0.08	0.692AspCys: 0.692 ± 0.03	3.644AspAsp: 3.644 ± 0.085	5.466AspGlu: 5.466 ± 0.093	3.038AspPhe: 3.038 ± 0.064	3.891AspGly: 3.891 ± 0.08	0.796AspHis: 0.796 ± 0.034	5.367AspIle: 5.367 ± 0.089	5.121AspLys: 5.121 ± 0.088	4.762AspLeu: 4.762 ± 0.091	1.802AspMet: 1.802 ± 0.048	3.092AspAsn: 3.092 ± 0.079	1.552AspPro: 1.552 ± 0.043	1.218AspGln: 1.218 ± 0.035	1.961AspArg: 1.961 ± 0.063	3.566AspSer: 3.566 ± 0.079	3.044AspThr: 3.044 ± 0.066	4.371AspVal: 4.371 ± 0.08	0.453AspTrp: 0.453 ± 0.027	3.14AspTyr: 3.14 ± 0.072	0.0AspXaa: 0.0 ± 0.0
Glu	4.802GluAla: 4.802 ± 0.091	0.818GluCys: 0.818 ± 0.031	4.432GluAsp: 4.432 ± 0.077	6.719GluGlu: 6.719 ± 0.134	2.94GluPhe: 2.94 ± 0.074	3.948GluGly: 3.948 ± 0.086	1.153GluHis: 1.153 ± 0.039	6.341GluIle: 6.341 ± 0.109	8.245GluLys: 8.245 ± 0.127	6.267GluLeu: 6.267 ± 0.109	2.259GluMet: 2.259 ± 0.062	5.136GluAsn: 5.136 ± 0.09	1.469GluPro: 1.469 ± 0.051	2.371GluGln: 2.371 ± 0.057	2.582GluArg: 2.582 ± 0.064	3.65GluSer: 3.65 ± 0.082	3.555GluThr: 3.555 ± 0.074	4.858GluVal: 4.858 ± 0.085	0.515GluTrp: 0.515 ± 0.024	3.261GluTyr: 3.261 ± 0.07	0.0GluXaa: 0.0 ± 0.0
Phe	2.794PheAla: 2.794 ± 0.059	0.65PheCys: 0.65 ± 0.03	2.822PheAsp: 2.822 ± 0.06	3.02PheGlu: 3.02 ± 0.067	2.042PhePhe: 2.042 ± 0.063	2.801PheGly: 2.801 ± 0.069	0.625PheHis: 0.625 ± 0.028	3.266PheIle: 3.266 ± 0.077	3.226PheLys: 3.226 ± 0.061	3.6PheLeu: 3.6 ± 0.073	1.263PheMet: 1.263 ± 0.037	2.159PheAsn: 2.159 ± 0.053	1.199PhePro: 1.199 ± 0.039	1.012PheGln: 1.012 ± 0.038	1.374PheArg: 1.374 ± 0.038	2.991PheSer: 2.991 ± 0.066	2.238PheThr: 2.238 ± 0.06	3.07PheVal: 3.07 ± 0.067	0.363PheTrp: 0.363 ± 0.022	1.812PheTyr: 1.812 ± 0.051	0.001PheXaa: 0.001 ± 0.001
Gly	4.321GlyAla: 4.321 ± 0.091	0.982GlyCys: 0.982 ± 0.038	3.409GlyAsp: 3.409 ± 0.061	4.087GlyGlu: 4.087 ± 0.07	2.959GlyPhe: 2.959 ± 0.06	3.991GlyGly: 3.991 ± 0.091	1.14GlyHis: 1.14 ± 0.039	5.744GlyIle: 5.744 ± 0.089	5.908GlyLys: 5.908 ± 0.091	5.039GlyLeu: 5.039 ± 0.084	2.036GlyMet: 2.036 ± 0.047	3.401GlyAsn: 3.401 ± 0.076	1.161GlyPro: 1.161 ± 0.045	1.876GlyGln: 1.876 ± 0.055	2.306GlyArg: 2.306 ± 0.054	3.593GlySer: 3.593 ± 0.074	3.593GlyThr: 3.593 ± 0.075	4.546GlyVal: 4.546 ± 0.082	0.52GlyTrp: 0.52 ± 0.028	3.128GlyTyr: 3.128 ± 0.064	0.0GlyXaa: 0.0 ± 0.0
His	0.825HisAla: 0.825 ± 0.036	0.261HisCys: 0.261 ± 0.021	0.919HisAsp: 0.919 ± 0.039	0.963HisGlu: 0.963 ± 0.037	0.809HisPhe: 0.809 ± 0.033	1.146HisGly: 1.146 ± 0.042	0.367HisHis: 0.367 ± 0.025	1.269HisIle: 1.269 ± 0.04	1.09HisLys: 1.09 ± 0.041	1.297HisLeu: 1.297 ± 0.041	0.477HisMet: 0.477 ± 0.025	0.897HisAsn: 0.897 ± 0.035	0.658HisPro: 0.658 ± 0.032	0.448HisGln: 0.448 ± 0.021	0.601HisArg: 0.601 ± 0.03	0.876HisSer: 0.876 ± 0.031	0.772HisThr: 0.772 ± 0.029	1.0HisVal: 1.0 ± 0.037	0.103HisTrp: 0.103 ± 0.013	0.721HisTyr: 0.721 ± 0.031	0.0HisXaa: 0.0 ± 0.0
Ile	5.75IleAla: 5.75 ± 0.097	1.274IleCys: 1.274 ± 0.045	5.092IleAsp: 5.092 ± 0.089	5.85IleGlu: 5.85 ± 0.097	3.535IlePhe: 3.535 ± 0.084	5.102IleGly: 5.102 ± 0.089	1.255IleHis: 1.255 ± 0.045	6.656IleIle: 6.656 ± 0.114	6.552IleLys: 6.552 ± 0.096	7.098IleLeu: 7.098 ± 0.129	2.234IleMet: 2.234 ± 0.057	4.336IleAsn: 4.336 ± 0.081	2.885IlePro: 2.885 ± 0.068	2.153IleGln: 2.153 ± 0.052	2.876IleArg: 2.876 ± 0.068	5.703IleSer: 5.703 ± 0.096	4.39IleThr: 4.39 ± 0.076	5.786IleVal: 5.786 ± 0.103	0.533IleTrp: 0.533 ± 0.026	3.229IleTyr: 3.229 ± 0.074	0.0IleXaa: 0.0 ± 0.0
Lys	5.682LysAla: 5.682 ± 0.085	0.831LysCys: 0.831 ± 0.031	5.588LysAsp: 5.588 ± 0.096	8.38LysGlu: 8.38 ± 0.138	2.969LysPhe: 2.969 ± 0.061	4.604LysGly: 4.604 ± 0.069	1.291LysHis: 1.291 ± 0.045	7.195LysIle: 7.195 ± 0.108	9.434LysLys: 9.434 ± 0.144	6.697LysLeu: 6.697 ± 0.095	2.612LysMet: 2.612 ± 0.058	6.093LysAsn: 6.093 ± 0.106	1.998LysPro: 1.998 ± 0.056	2.563LysGln: 2.563 ± 0.058	3.077LysArg: 3.077 ± 0.063	4.55LysSer: 4.55 ± 0.084	4.475LysThr: 4.475 ± 0.085	5.909LysVal: 5.909 ± 0.092	0.645LysTrp: 0.645 ± 0.032	4.058LysTyr: 4.058 ± 0.078	0.0LysXaa: 0.0 ± 0.0
Leu	5.599LeuAla: 5.599 ± 0.084	1.166LeuCys: 1.166 ± 0.041	5.412LeuAsp: 5.412 ± 0.094	6.315LeuGlu: 6.315 ± 0.121	3.463LeuPhe: 3.463 ± 0.087	5.404LeuGly: 5.404 ± 0.089	1.22LeuHis: 1.22 ± 0.043	6.059LeuIle: 6.059 ± 0.108	7.113LeuLys: 7.113 ± 0.104	6.851LeuLeu: 6.851 ± 0.122	2.188LeuMet: 2.188 ± 0.056	4.525LeuAsn: 4.525 ± 0.077	2.656LeuPro: 2.656 ± 0.059	2.393LeuGln: 2.393 ± 0.057	2.85LeuArg: 2.85 ± 0.059	5.698LeuSer: 5.698 ± 0.105	4.362LeuThr: 4.362 ± 0.067	5.395LeuVal: 5.395 ± 0.084	0.551LeuTrp: 0.551 ± 0.027	3.117LeuTyr: 3.117 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.117MetAla: 2.117 ± 0.059	0.391MetCys: 0.391 ± 0.02	1.82MetAsp: 1.82 ± 0.042	2.114MetGlu: 2.114 ± 0.055	1.069MetPhe: 1.069 ± 0.038	1.811MetGly: 1.811 ± 0.046	0.467MetHis: 0.467 ± 0.024	2.193MetIle: 2.193 ± 0.058	2.572MetLys: 2.572 ± 0.056	2.509MetLeu: 2.509 ± 0.064	0.806MetMet: 0.806 ± 0.035	1.627MetAsn: 1.627 ± 0.042	1.08MetPro: 1.08 ± 0.035	0.956MetGln: 0.956 ± 0.033	1.098MetArg: 1.098 ± 0.036	1.833MetSer: 1.833 ± 0.048	1.555MetThr: 1.555 ± 0.043	1.809MetVal: 1.809 ± 0.048	0.201MetTrp: 0.201 ± 0.017	1.072MetTyr: 1.072 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.415AsnAla: 3.415 ± 0.071	0.697AsnCys: 0.697 ± 0.036	3.168AsnAsp: 3.168 ± 0.062	3.907AsnGlu: 3.907 ± 0.073	2.252AsnPhe: 2.252 ± 0.058	4.015AsnGly: 4.015 ± 0.087	0.934AsnHis: 0.934 ± 0.037	4.802AsnIle: 4.802 ± 0.081	4.674AsnLys: 4.674 ± 0.105	4.537AsnLeu: 4.537 ± 0.081	1.691AsnMet: 1.691 ± 0.048	3.487AsnAsn: 3.487 ± 0.114	2.088AsnPro: 2.088 ± 0.061	1.795AsnGln: 1.795 ± 0.063	2.025AsnArg: 2.025 ± 0.06	3.497AsnSer: 3.497 ± 0.091	2.901AsnThr: 2.901 ± 0.063	3.838AsnVal: 3.838 ± 0.068	0.418AsnTrp: 0.418 ± 0.021	2.558AsnTyr: 2.558 ± 0.07	0.0AsnXaa: 0.0 ± 0.0
Pro	1.702ProAla: 1.702 ± 0.052	0.358ProCys: 0.358 ± 0.019	1.741ProAsp: 1.741 ± 0.049	2.426ProGlu: 2.426 ± 0.062	1.302ProPhe: 1.302 ± 0.044	1.729ProGly: 1.729 ± 0.051	0.481ProHis: 0.481 ± 0.025	2.353ProIle: 2.353 ± 0.064	2.163ProLys: 2.163 ± 0.053	2.194ProLeu: 2.194 ± 0.048	0.784ProMet: 0.784 ± 0.033	1.49ProAsn: 1.49 ± 0.053	0.497ProPro: 0.497 ± 0.028	0.902ProGln: 0.902 ± 0.037	0.842ProArg: 0.842 ± 0.033	1.633ProSer: 1.633 ± 0.044	1.599ProThr: 1.599 ± 0.047	2.178ProVal: 2.178 ± 0.062	0.258ProTrp: 0.258 ± 0.018	1.271ProTyr: 1.271 ± 0.037	0.001ProXaa: 0.001 ± 0.001
Gln	1.827GlnAla: 1.827 ± 0.05	0.296GlnCys: 0.296 ± 0.02	1.459GlnAsp: 1.459 ± 0.044	2.233GlnGlu: 2.233 ± 0.058	1.153GlnPhe: 1.153 ± 0.037	1.763GlnGly: 1.763 ± 0.053	0.467GlnHis: 0.467 ± 0.022	2.407GlnIle: 2.407 ± 0.057	2.84GlnLys: 2.84 ± 0.064	2.564GlnLeu: 2.564 ± 0.062	1.001GlnMet: 1.001 ± 0.04	1.687GlnAsn: 1.687 ± 0.046	0.737GlnPro: 0.737 ± 0.034	1.123GlnGln: 1.123 ± 0.053	1.114GlnArg: 1.114 ± 0.041	1.578GlnSer: 1.578 ± 0.047	1.464GlnThr: 1.464 ± 0.048	1.821GlnVal: 1.821 ± 0.048	0.249GlnTrp: 0.249 ± 0.018	1.248GlnTyr: 1.248 ± 0.042	0.0GlnXaa: 0.0 ± 0.0
Arg	2.037ArgAla: 2.037 ± 0.051	0.425ArgCys: 0.425 ± 0.026	1.994ArgAsp: 1.994 ± 0.049	2.86ArgGlu: 2.86 ± 0.072	1.534ArgPhe: 1.534 ± 0.045	1.963ArgGly: 1.963 ± 0.054	0.627ArgHis: 0.627 ± 0.028	2.991ArgIle: 2.991 ± 0.066	3.433ArgLys: 3.433 ± 0.066	2.967ArgLeu: 2.967 ± 0.067	1.135ArgMet: 1.135 ± 0.037	1.975ArgAsn: 1.975 ± 0.058	0.98ArgPro: 0.98 ± 0.035	1.255ArgGln: 1.255 ± 0.04	1.615ArgArg: 1.615 ± 0.052	1.616ArgSer: 1.616 ± 0.046	1.729ArgThr: 1.729 ± 0.042	2.215ArgVal: 2.215 ± 0.064	0.266ArgTrp: 0.266 ± 0.018	1.561ArgTyr: 1.561 ± 0.048	0.0ArgXaa: 0.0 ± 0.0
Ser	3.882SerAla: 3.882 ± 0.074	0.654SerCys: 0.654 ± 0.033	3.784SerAsp: 3.784 ± 0.077	4.115SerGlu: 4.115 ± 0.071	2.567SerPhe: 2.567 ± 0.058	4.141SerGly: 4.141 ± 0.072	0.926SerHis: 0.926 ± 0.038	4.815SerIle: 4.815 ± 0.089	5.402SerLys: 5.402 ± 0.091	4.959SerLeu: 4.959 ± 0.09	1.717SerMet: 1.717 ± 0.048	3.518SerAsn: 3.518 ± 0.085	1.452SerPro: 1.452 ± 0.041	1.964SerGln: 1.964 ± 0.05	2.18SerArg: 2.18 ± 0.05	4.013SerSer: 4.013 ± 0.099	3.206SerThr: 3.206 ± 0.075	4.188SerVal: 4.188 ± 0.079	0.426SerTrp: 0.426 ± 0.023	2.694SerTyr: 2.694 ± 0.062	0.001SerXaa: 0.001 ± 0.001
Thr	3.475ThrAla: 3.475 ± 0.079	0.601ThrCys: 0.601 ± 0.027	3.107ThrAsp: 3.107 ± 0.063	3.426ThrGlu: 3.426 ± 0.073	2.263ThrPhe: 2.263 ± 0.053	3.843ThrGly: 3.843 ± 0.081	0.815ThrHis: 0.815 ± 0.034	4.534ThrIle: 4.534 ± 0.088	4.249ThrLys: 4.249 ± 0.076	4.505ThrLeu: 4.505 ± 0.081	1.418ThrMet: 1.418 ± 0.044	2.718ThrAsn: 2.718 ± 0.065	1.907ThrPro: 1.907 ± 0.048	1.442ThrGln: 1.442 ± 0.046	1.793ThrArg: 1.793 ± 0.045	3.312ThrSer: 3.312 ± 0.065	3.179ThrThr: 3.179 ± 0.079	3.951ThrVal: 3.951 ± 0.077	0.415ThrTrp: 0.415 ± 0.02	2.026ThrTyr: 2.026 ± 0.041	0.0ThrXaa: 0.0 ± 0.0
Val	4.882ValAla: 4.882 ± 0.088	1.08ValCys: 1.08 ± 0.037	4.422ValAsp: 4.422 ± 0.079	4.986ValGlu: 4.986 ± 0.087	2.913ValPhe: 2.913 ± 0.065	4.432ValGly: 4.432 ± 0.084	0.946ValHis: 0.946 ± 0.038	5.555ValIle: 5.555 ± 0.082	5.623ValLys: 5.623 ± 0.082	5.823ValLeu: 5.823 ± 0.097	1.828ValMet: 1.828 ± 0.049	3.505ValAsn: 3.505 ± 0.066	2.071ValPro: 2.071 ± 0.05	1.69ValGln: 1.69 ± 0.045	2.25ValArg: 2.25 ± 0.046	4.644ValSer: 4.644 ± 0.079	3.974ValThr: 3.974 ± 0.079	5.236ValVal: 5.236 ± 0.105	0.475ValTrp: 0.475 ± 0.024	2.755ValTyr: 2.755 ± 0.068	0.0ValXaa: 0.0 ± 0.0
Trp	0.388TrpAla: 0.388 ± 0.026	0.106TrpCys: 0.106 ± 0.012	0.45TrpAsp: 0.45 ± 0.029	0.444TrpGlu: 0.444 ± 0.022	0.334TrpPhe: 0.334 ± 0.022	0.51TrpGly: 0.51 ± 0.025	0.147TrpHis: 0.147 ± 0.014	0.594TrpIle: 0.594 ± 0.031	0.628TrpLys: 0.628 ± 0.026	0.658TrpLeu: 0.658 ± 0.033	0.236TrpMet: 0.236 ± 0.02	0.486TrpAsn: 0.486 ± 0.025	0.185TrpPro: 0.185 ± 0.017	0.291TrpGln: 0.291 ± 0.022	0.25TrpArg: 0.25 ± 0.018	0.413TrpSer: 0.413 ± 0.024	0.337TrpThr: 0.337 ± 0.02	0.429TrpVal: 0.429 ± 0.024	0.074TrpTrp: 0.074 ± 0.011	0.321TrpTyr: 0.321 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.515TyrAla: 2.515 ± 0.066	0.604TyrCys: 0.604 ± 0.027	2.962TyrAsp: 2.962 ± 0.062	3.119TyrGlu: 3.119 ± 0.065	1.956TyrPhe: 1.956 ± 0.06	2.823TyrGly: 2.823 ± 0.072	0.692TyrHis: 0.692 ± 0.032	3.275TyrIle: 3.275 ± 0.066	3.453TyrLys: 3.453 ± 0.068	3.374TyrLeu: 3.374 ± 0.065	1.161TyrMet: 1.161 ± 0.04	2.622TyrAsn: 2.622 ± 0.069	1.232TyrPro: 1.232 ± 0.043	1.282TyrGln: 1.282 ± 0.043	1.667TyrArg: 1.667 ± 0.051	2.788TyrSer: 2.788 ± 0.069	2.288TyrThr: 2.288 ± 0.051	2.838TyrVal: 2.838 ± 0.052	0.301TyrTrp: 0.301 ± 0.018	2.021TyrTyr: 2.021 ± 0.059	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.002	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.01XaaXaa: 0.01 ± 0.005

Statistics based on 2493 proteins (815008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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