Marinimicrobium koreense

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Marinimicrobium

Average proteome isoelectric point is 5.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3203 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1P249|A0A3N1P249_9GAMM Flavin-dependent dehydrogenase OS=Marinimicrobium koreense OX=306545 GN=EDC38_2304 PE=4 SV=1
MM1 pKa = 7.7KK2 pKa = 10.19YY3 pKa = 9.57IASAVLAAALLAPAYY18 pKa = 9.62GAVAAAPSNLALDD31 pKa = 4.41KK32 pKa = 10.48PVSASSSQNRR42 pKa = 11.84NRR44 pKa = 11.84DD45 pKa = 2.85AEE47 pKa = 4.12FAVDD51 pKa = 3.86GNVGTRR57 pKa = 11.84WSSDD61 pKa = 3.11FSDD64 pKa = 4.23GNWIIVDD71 pKa = 4.32LEE73 pKa = 4.23SVYY76 pKa = 10.82DD77 pKa = 3.64ISSVILQWEE86 pKa = 4.04TAYY89 pKa = 10.05PEE91 pKa = 4.14QYY93 pKa = 10.61NLEE96 pKa = 4.1VSLDD100 pKa = 3.54GSNWEE105 pKa = 4.18TVSAVTSSNGGVDD118 pKa = 3.49SFAMTRR124 pKa = 11.84SARR127 pKa = 11.84YY128 pKa = 9.73VRR130 pKa = 11.84VEE132 pKa = 3.94SVKK135 pKa = 10.57RR136 pKa = 11.84ATKK139 pKa = 9.52WGVSLWEE146 pKa = 3.95LEE148 pKa = 4.54VYY150 pKa = 10.22GVEE153 pKa = 4.37PEE155 pKa = 4.52PEE157 pKa = 4.32PEE159 pKa = 4.35PEE161 pKa = 4.37PEE163 pKa = 4.45PEE165 pKa = 4.62PEE167 pKa = 4.25TEE169 pKa = 4.35PEE171 pKa = 4.12STTDD175 pKa = 3.67SEE177 pKa = 4.77TTSGDD182 pKa = 3.52GANLALNQTMFASSSEE198 pKa = 4.04NSSRR202 pKa = 11.84LPEE205 pKa = 4.04EE206 pKa = 4.97AVDD209 pKa = 3.68GDD211 pKa = 4.26MNSRR215 pKa = 11.84WASEE219 pKa = 3.98YY220 pKa = 11.51GEE222 pKa = 4.48DD223 pKa = 3.34QWIAVDD229 pKa = 5.43LGATYY234 pKa = 10.88DD235 pKa = 3.82LSSLILRR242 pKa = 11.84WEE244 pKa = 4.08VAHH247 pKa = 7.22AEE249 pKa = 4.24DD250 pKa = 5.01YY251 pKa = 10.89DD252 pKa = 3.99IQVSADD258 pKa = 3.35ANDD261 pKa = 3.42WQTAQSVTNSDD272 pKa = 3.01GGEE275 pKa = 4.03DD276 pKa = 3.8VVEE279 pKa = 4.67LSATGRR285 pKa = 11.84YY286 pKa = 7.77VRR288 pKa = 11.84IQSVRR293 pKa = 11.84RR294 pKa = 11.84ATQWGISLWEE304 pKa = 4.01LEE306 pKa = 4.59VYY308 pKa = 10.2GAEE311 pKa = 4.34PYY313 pKa = 9.86VAPEE317 pKa = 3.9PQLVSEE323 pKa = 4.92GRR325 pKa = 11.84PATASSTEE333 pKa = 3.79RR334 pKa = 11.84DD335 pKa = 3.21AYY337 pKa = 10.72APEE340 pKa = 3.89NAFDD344 pKa = 4.32GSGSTRR350 pKa = 11.84WASVYY355 pKa = 11.07GDD357 pKa = 3.94TAWLQVDD364 pKa = 4.56LGEE367 pKa = 4.21AHH369 pKa = 6.92QITGINLDD377 pKa = 3.23WEE379 pKa = 4.51TAYY382 pKa = 10.95SSEE385 pKa = 4.03YY386 pKa = 10.25QIQTSYY392 pKa = 11.5DD393 pKa = 3.58GQSWTTVEE401 pKa = 5.16HH402 pKa = 6.61IVDD405 pKa = 4.02GNGGTDD411 pKa = 3.32EE412 pKa = 4.85LVVDD416 pKa = 3.26GHH418 pKa = 5.56GRR420 pKa = 11.84YY421 pKa = 9.29VRR423 pKa = 11.84MMGLARR429 pKa = 11.84ATSWGHH435 pKa = 5.82SLWEE439 pKa = 4.07MQVFGYY445 pKa = 9.52PADD448 pKa = 4.18SADD451 pKa = 3.89SPSDD455 pKa = 3.51GTDD458 pKa = 2.7GSTDD462 pKa = 3.6DD463 pKa = 4.07GTGSEE468 pKa = 4.72TDD470 pKa = 3.35TGSDD474 pKa = 3.38TDD476 pKa = 3.64TGSDD480 pKa = 4.18DD481 pKa = 3.92GSTGDD486 pKa = 3.5GSTGGEE492 pKa = 3.83EE493 pKa = 4.13STEE496 pKa = 4.05EE497 pKa = 4.03EE498 pKa = 4.68TVDD501 pKa = 3.37TTAPSAPGSLNDD513 pKa = 3.78TAVGTSYY520 pKa = 9.48VTLSWSEE527 pKa = 4.05STDD530 pKa = 3.24NEE532 pKa = 3.91AVMSYY537 pKa = 9.57EE538 pKa = 4.08VYY540 pKa = 10.51RR541 pKa = 11.84DD542 pKa = 3.35GARR545 pKa = 11.84ITEE548 pKa = 4.37LLAPEE553 pKa = 4.48TSYY556 pKa = 11.7TDD558 pKa = 3.17TGLSSGTTYY567 pKa = 10.45QYY569 pKa = 11.18SVRR572 pKa = 11.84AGDD575 pKa = 3.97AAGNWSEE582 pKa = 3.98NSTTLVVTTNSEE594 pKa = 4.12QVVGDD599 pKa = 3.79GVVLEE604 pKa = 4.63WTTPDD609 pKa = 3.05QRR611 pKa = 11.84EE612 pKa = 3.99NGSYY616 pKa = 11.1LEE618 pKa = 4.15LNEE621 pKa = 3.8IGGYY625 pKa = 7.67EE626 pKa = 3.78IRR628 pKa = 11.84YY629 pKa = 7.61QQGADD634 pKa = 3.56GEE636 pKa = 4.71SQSVVVNDD644 pKa = 4.9AYY646 pKa = 10.42ATSHH650 pKa = 6.05EE651 pKa = 4.82LNLEE655 pKa = 4.11DD656 pKa = 5.56GIYY659 pKa = 9.79EE660 pKa = 3.75FSIAVFDD667 pKa = 4.17VNGLYY672 pKa = 10.53SQFVPIDD679 pKa = 3.64PVPP682 pKa = 3.5

Molecular weight: 73.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1P4B2|A0A3N1P4B2_9GAMM Carbohydrate binding protein with CBM6 domain OS=Marinimicrobium koreense OX=306545 GN=EDC38_0261 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.7ALAVV44 pKa = 3.49

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3203

0

3203

1151955

34

4537

359.6

39.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.665 ± 0.048

0.877 ± 0.013

6.172 ± 0.037

6.781 ± 0.037

3.688 ± 0.029

7.737 ± 0.045

2.295 ± 0.025

4.797 ± 0.029

3.277 ± 0.036

10.644 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.021

3.344 ± 0.031

4.802 ± 0.03

4.357 ± 0.03

6.505 ± 0.045

6.033 ± 0.04

5.239 ± 0.035

7.067 ± 0.036

1.55 ± 0.022

2.907 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski