Poecile atricapillus GI tract-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus chickad1; Chickadee associated gemycircularvirus 1

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K1P922|A0A0K1P922_9VIRU Putative capsid protein OS=Poecile atricapillus GI tract-associated gemycircularvirus OX=1699094 PE=4 SV=1
MM1 pKa = 7.39LGDD4 pKa = 3.52VGVLIVRR11 pKa = 11.84PVSCVLLYY19 pKa = 10.86NISFQQDD26 pKa = 3.36SEE28 pKa = 4.56DD29 pKa = 3.27TFMPSIHH36 pKa = 6.46FCAQHH41 pKa = 6.27FLLTYY46 pKa = 10.3AHH48 pKa = 6.86SEE50 pKa = 4.25GNDD53 pKa = 3.3VKK55 pKa = 11.21PEE57 pKa = 4.19LDD59 pKa = 3.07PFRR62 pKa = 11.84IVEE65 pKa = 4.06VLGALGAEE73 pKa = 4.39CIVARR78 pKa = 11.84EE79 pKa = 4.11YY80 pKa = 11.55YY81 pKa = 6.81PTTVGFHH88 pKa = 5.84LHH90 pKa = 5.73VFCSFEE96 pKa = 3.72RR97 pKa = 11.84RR98 pKa = 11.84FRR100 pKa = 11.84SRR102 pKa = 11.84KK103 pKa = 8.9IDD105 pKa = 3.35VFDD108 pKa = 3.49VDD110 pKa = 5.11GYY112 pKa = 11.15HH113 pKa = 7.21PNAEE117 pKa = 4.19PSRR120 pKa = 11.84KK121 pKa = 9.03NALGGYY127 pKa = 9.94DD128 pKa = 3.68YY129 pKa = 10.81AIKK132 pKa = 10.7DD133 pKa = 3.82GEE135 pKa = 4.57VVAGGLEE142 pKa = 4.15RR143 pKa = 11.84PSGVSGRR150 pKa = 11.84RR151 pKa = 11.84GPNRR155 pKa = 11.84SADD158 pKa = 3.07EE159 pKa = 3.96WAEE162 pKa = 3.85ITASEE167 pKa = 4.26TVEE170 pKa = 4.19EE171 pKa = 4.78FWRR174 pKa = 11.84LCEE177 pKa = 3.95EE178 pKa = 5.05LDD180 pKa = 4.39PKK182 pKa = 11.25SLVCNFPALLRR193 pKa = 11.84FSEE196 pKa = 4.01WRR198 pKa = 11.84FRR200 pKa = 11.84PVEE203 pKa = 3.99IPYY206 pKa = 8.14ATPDD210 pKa = 3.48GVFDD214 pKa = 4.17LAGYY218 pKa = 8.96PDD220 pKa = 3.83LADD223 pKa = 3.15WRR225 pKa = 11.84DD226 pKa = 3.3NVLFGGGSGEE236 pKa = 5.32SPFLWAEE243 pKa = 3.62QTKK246 pKa = 10.27IVV248 pKa = 3.77

Molecular weight: 27.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K1P922|A0A0K1P922_9VIRU Putative capsid protein OS=Poecile atricapillus GI tract-associated gemycircularvirus OX=1699094 PE=4 SV=1
MM1 pKa = 7.02PRR3 pKa = 11.84KK4 pKa = 9.45YY5 pKa = 10.47LSRR8 pKa = 11.84YY9 pKa = 7.23RR10 pKa = 11.84PKK12 pKa = 10.72YY13 pKa = 8.16SGAYY17 pKa = 8.66RR18 pKa = 11.84GRR20 pKa = 11.84RR21 pKa = 11.84PVRR24 pKa = 11.84AKK26 pKa = 10.19ASPRR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84SYY34 pKa = 10.18GRR36 pKa = 11.84KK37 pKa = 8.97RR38 pKa = 11.84YY39 pKa = 9.45GGKK42 pKa = 6.68TTSKK46 pKa = 10.63KK47 pKa = 10.44RR48 pKa = 11.84ILNLTSIKK56 pKa = 10.55KK57 pKa = 9.7RR58 pKa = 11.84DD59 pKa = 3.49TMLAYY64 pKa = 9.58YY65 pKa = 10.48QNASTVNPQVGGYY78 pKa = 6.49TLQGTGGGNGGPATMLMWCATARR101 pKa = 11.84DD102 pKa = 3.86NTVAMGGSLGNRR114 pKa = 11.84FTPSTRR120 pKa = 11.84TATTCYY126 pKa = 9.76MRR128 pKa = 11.84GLSEE132 pKa = 4.69NIEE135 pKa = 4.12LSSNSGVPWQWRR147 pKa = 11.84RR148 pKa = 11.84ICFTFRR154 pKa = 11.84DD155 pKa = 4.17PASLSTVQSFYY166 pKa = 11.7NEE168 pKa = 4.15GTSGFQRR175 pKa = 11.84LLYY178 pKa = 10.26NLTSGSSPDD187 pKa = 3.52NVNIANVRR195 pKa = 11.84GLLFRR200 pKa = 11.84GEE202 pKa = 4.82LGQDD206 pKa = 2.61WASYY210 pKa = 8.31LTAPTDD216 pKa = 3.47NSRR219 pKa = 11.84VTIKK223 pKa = 9.75MDD225 pKa = 3.05KK226 pKa = 10.06TRR228 pKa = 11.84TISSGNQSGRR238 pKa = 11.84MVYY241 pKa = 10.57QKK243 pKa = 9.47MWMPMNKK250 pKa = 9.51NLVYY254 pKa = 10.83DD255 pKa = 4.2DD256 pKa = 5.05DD257 pKa = 4.91EE258 pKa = 6.32NGGSEE263 pKa = 3.98TARR266 pKa = 11.84FFSVGGKK273 pKa = 10.28AGMGDD278 pKa = 4.25YY279 pKa = 10.75YY280 pKa = 11.5VVDD283 pKa = 3.99IFQAGAGAVSSDD295 pKa = 3.08TLLFNPQATLYY306 pKa = 8.79WHH308 pKa = 6.98EE309 pKa = 4.28KK310 pKa = 9.3

Molecular weight: 34.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

558

248

310

279.0

31.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.631 ± 0.15

1.613 ± 0.541

5.376 ± 0.991

5.018 ± 2.313

4.839 ± 1.352

9.857 ± 0.931

1.254 ± 0.781

3.226 ± 0.541

3.763 ± 0.901

7.527 ± 0.631

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.509 ± 1.142

4.659 ± 1.232

5.197 ± 0.841

2.688 ± 0.721

8.065 ± 0.811

8.065 ± 1.082

6.272 ± 2.043

6.631 ± 1.502

1.971 ± 0.03

4.839 ± 0.811
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski