Rhizoctonia fumigata mycovirus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6Q4A5|A0A0F6Q4A5_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia fumigata mycovirus OX=1592828 PE=4 SV=1
MM1 pKa = 7.67DD2 pKa = 4.7HH3 pKa = 7.13ARR5 pKa = 11.84DD6 pKa = 3.49FDD8 pKa = 4.73VISSNATNPLKK19 pKa = 10.58RR20 pKa = 11.84SRR22 pKa = 11.84EE23 pKa = 3.94EE24 pKa = 3.98ADD26 pKa = 3.39LGTAGEE32 pKa = 4.5APWRR36 pKa = 11.84TPVNGATINGVFRR49 pKa = 11.84IRR51 pKa = 11.84KK52 pKa = 9.16FDD54 pKa = 3.38VDD56 pKa = 4.0YY57 pKa = 11.63LNGPVADD64 pKa = 4.21AEE66 pKa = 4.58VVIAGLPRR74 pKa = 11.84VKK76 pKa = 10.31EE77 pKa = 4.15VVPEE81 pKa = 4.12SCDD84 pKa = 3.11ALRR87 pKa = 11.84GMKK90 pKa = 9.74VLNDD94 pKa = 3.64ADD96 pKa = 4.86HH97 pKa = 6.5ITTAVSSEE105 pKa = 4.22SLSGTGNGGTRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84NSRR121 pKa = 11.84RR122 pKa = 11.84SRR124 pKa = 11.84KK125 pKa = 9.9NKK127 pKa = 9.7VKK129 pKa = 8.44TTAKK133 pKa = 10.37SGGSLDD139 pKa = 4.27DD140 pKa = 3.86LKK142 pKa = 11.68EE143 pKa = 4.07MFKK146 pKa = 10.76HH147 pKa = 5.1ITVHH151 pKa = 6.61DD152 pKa = 4.01AQVRR156 pKa = 11.84LGKK159 pKa = 10.24FHH161 pKa = 7.8DD162 pKa = 5.16EE163 pKa = 4.06ITFTPADD170 pKa = 3.95PAVLGYY176 pKa = 9.93LQSPEE181 pKa = 4.05GEE183 pKa = 4.01IPGFDD188 pKa = 3.33PSEE191 pKa = 4.22YY192 pKa = 10.69CFMEE196 pKa = 4.4ASGGVQMRR204 pKa = 11.84HH205 pKa = 5.06LKK207 pKa = 9.94MYY209 pKa = 10.92DD210 pKa = 3.35EE211 pKa = 4.93DD212 pKa = 4.22TSLGPDD218 pKa = 4.05FSLLEE223 pKa = 4.42DD224 pKa = 4.02PEE226 pKa = 4.75AGRR229 pKa = 11.84RR230 pKa = 11.84ALEE233 pKa = 3.7HH234 pKa = 7.02AIDD237 pKa = 4.0HH238 pKa = 5.69VQDD241 pKa = 4.66LLRR244 pKa = 11.84LPNKK248 pKa = 9.94LPRR251 pKa = 11.84VDD253 pKa = 5.57RR254 pKa = 11.84IPLEE258 pKa = 4.13GIRR261 pKa = 11.84YY262 pKa = 8.43DD263 pKa = 3.4KK264 pKa = 11.24SKK266 pKa = 11.07SCGAHH271 pKa = 5.28YY272 pKa = 10.41RR273 pKa = 11.84LQGFKK278 pKa = 10.11TRR280 pKa = 11.84GDD282 pKa = 3.26VWEE285 pKa = 4.11VAMFEE290 pKa = 4.39AKK292 pKa = 10.11NALVKK297 pKa = 10.65LWDD300 pKa = 3.81GEE302 pKa = 4.58YY303 pKa = 10.7VEE305 pKa = 5.04PRR307 pKa = 11.84PTRR310 pKa = 11.84MGGRR314 pKa = 11.84GKK316 pKa = 9.96LVKK319 pKa = 9.9MSQEE323 pKa = 3.68RR324 pKa = 11.84AVAEE328 pKa = 4.53GVAKK332 pKa = 10.47GRR334 pKa = 11.84AIHH337 pKa = 5.52MTDD340 pKa = 3.04TRR342 pKa = 11.84DD343 pKa = 3.67HH344 pKa = 6.97IILGLSEE351 pKa = 4.55QPLNDD356 pKa = 2.71AWKK359 pKa = 9.41PDD361 pKa = 3.64NFPISVGRR369 pKa = 11.84GWFHH373 pKa = 7.0GDD375 pKa = 2.53ATRR378 pKa = 11.84FVRR381 pKa = 11.84KK382 pKa = 8.79HH383 pKa = 5.75AGASRR388 pKa = 11.84IYY390 pKa = 10.84CFDD393 pKa = 3.39AEE395 pKa = 4.73KK396 pKa = 10.25FDD398 pKa = 4.21SSIMPWLIHH407 pKa = 5.77IAVTIMRR414 pKa = 11.84EE415 pKa = 3.69QFMEE419 pKa = 4.32GLRR422 pKa = 11.84HH423 pKa = 6.15DD424 pKa = 5.21ADD426 pKa = 4.11MYY428 pKa = 8.37WQFVEE433 pKa = 4.82EE434 pKa = 4.47SLLHH438 pKa = 7.06SMVFRR443 pKa = 11.84DD444 pKa = 4.73DD445 pKa = 3.68GVLFEE450 pKa = 5.35KK451 pKa = 11.12YY452 pKa = 9.68HH453 pKa = 5.45GTSSGHH459 pKa = 4.98NHH461 pKa = 6.0NSLAQSIVTCILASFNVFYY480 pKa = 10.88KK481 pKa = 10.69NRR483 pKa = 11.84EE484 pKa = 3.93LPVAVIKK491 pKa = 11.12ANFTIEE497 pKa = 4.3GLGDD501 pKa = 3.95DD502 pKa = 5.39NITCEE507 pKa = 4.17TDD509 pKa = 3.13VLADD513 pKa = 3.76EE514 pKa = 4.66TCEE517 pKa = 3.87EE518 pKa = 4.04RR519 pKa = 11.84GMRR522 pKa = 11.84TWNVFGVSWLGEE534 pKa = 3.77KK535 pKa = 10.34SFQTNTLCQPVVDD548 pKa = 3.81EE549 pKa = 5.23AEE551 pKa = 3.9WDD553 pKa = 3.7EE554 pKa = 4.26EE555 pKa = 4.68GMFGSAQYY563 pKa = 10.27LGKK566 pKa = 10.42FFRR569 pKa = 11.84EE570 pKa = 4.41DD571 pKa = 3.36VLEE574 pKa = 4.35VPSLGQVKK582 pKa = 9.16VVTPYY587 pKa = 10.04RR588 pKa = 11.84PQVEE592 pKa = 4.37TVLRR596 pKa = 11.84LLYY599 pKa = 10.16PEE601 pKa = 5.19GIMRR605 pKa = 11.84GDD607 pKa = 3.02AHH609 pKa = 7.63GDD611 pKa = 3.35FEE613 pKa = 5.22EE614 pKa = 4.48VFEE617 pKa = 4.25RR618 pKa = 11.84ARR620 pKa = 11.84GEE622 pKa = 3.99RR623 pKa = 11.84FAGHH627 pKa = 6.82ILDD630 pKa = 4.32GSGNPRR636 pKa = 11.84TRR638 pKa = 11.84AWLDD642 pKa = 3.9GYY644 pKa = 9.39WQFCHH649 pKa = 7.27DD650 pKa = 4.15NLLVIEE656 pKa = 4.39LTGHH660 pKa = 6.28HH661 pKa = 6.14SLEE664 pKa = 4.06KK665 pKa = 10.13RR666 pKa = 11.84LARR669 pKa = 11.84LGVEE673 pKa = 3.93IDD675 pKa = 3.42VDD677 pKa = 3.81DD678 pKa = 4.67AVYY681 pKa = 11.13DD682 pKa = 4.25DD683 pKa = 5.4GTAGWLRR690 pKa = 11.84LVNRR694 pKa = 11.84RR695 pKa = 11.84RR696 pKa = 11.84DD697 pKa = 3.87GNWEE701 pKa = 3.7FLQIVV706 pKa = 3.79

Molecular weight: 79.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6Q4A5|A0A0F6Q4A5_9VIRU Putative RNA-dependent RNA polymerase OS=Rhizoctonia fumigata mycovirus OX=1592828 PE=4 SV=1
MM1 pKa = 7.27SGSMEE6 pKa = 4.39DD7 pKa = 4.46FEE9 pKa = 4.85ATLSRR14 pKa = 11.84VMGRR18 pKa = 11.84IGNVTNAPEE27 pKa = 3.76RR28 pKa = 11.84AEE30 pKa = 4.3TIEE33 pKa = 4.15EE34 pKa = 4.07AASQPRR40 pKa = 11.84APATTSVGVAKK51 pKa = 10.73EE52 pKa = 4.0EE53 pKa = 4.3DD54 pKa = 3.78SLPLVRR60 pKa = 11.84EE61 pKa = 3.97DD62 pKa = 3.38MTLKK66 pKa = 10.35EE67 pKa = 4.17LRR69 pKa = 11.84EE70 pKa = 3.96VMEE73 pKa = 4.52KK74 pKa = 10.13YY75 pKa = 10.68GKK77 pKa = 9.58VVEE80 pKa = 4.63RR81 pKa = 11.84GAYY84 pKa = 9.4LYY86 pKa = 10.28EE87 pKa = 3.96WTGGKK92 pKa = 9.13AAKK95 pKa = 10.48LNTLDD100 pKa = 3.74PKK102 pKa = 10.47EE103 pKa = 4.15LGRR106 pKa = 11.84EE107 pKa = 3.68RR108 pKa = 11.84MTSQEE113 pKa = 3.68RR114 pKa = 11.84RR115 pKa = 11.84ALDD118 pKa = 2.81WWEE121 pKa = 3.84EE122 pKa = 3.93KK123 pKa = 10.65GKK125 pKa = 10.88KK126 pKa = 9.72NEE128 pKa = 4.26FDD130 pKa = 3.12WTVCRR135 pKa = 11.84EE136 pKa = 4.1SGKK139 pKa = 10.28VPAGKK144 pKa = 9.3IGKK147 pKa = 9.71YY148 pKa = 9.43NDD150 pKa = 3.49EE151 pKa = 4.2LSAMRR156 pKa = 11.84WMYY159 pKa = 10.35QNKK162 pKa = 9.53ALTMEE167 pKa = 3.68NWFWLRR173 pKa = 11.84KK174 pKa = 7.74HH175 pKa = 6.25HH176 pKa = 6.07EE177 pKa = 4.3ARR179 pKa = 11.84SHH181 pKa = 5.26VVWALVKK188 pKa = 10.01IVKK191 pKa = 9.64AAQDD195 pKa = 3.6VTNGMDD201 pKa = 3.01TSDD204 pKa = 3.56ALGVAEE210 pKa = 4.41YY211 pKa = 8.74QTANKK216 pKa = 9.16IISIASTNVKK226 pKa = 9.03TFMEE230 pKa = 4.48QIHH233 pKa = 6.34RR234 pKa = 11.84LLRR237 pKa = 11.84PIDD240 pKa = 3.68EE241 pKa = 4.58GLNLVVARR249 pKa = 11.84KK250 pKa = 9.1IAMGEE255 pKa = 3.81RR256 pKa = 11.84LGIKK260 pKa = 8.39QKK262 pKa = 10.93KK263 pKa = 9.07VGTANRR269 pKa = 11.84QAAMFGEE276 pKa = 4.48EE277 pKa = 3.81AVGRR281 pKa = 11.84VTKK284 pKa = 10.47PSDD287 pKa = 3.36VPRR290 pKa = 11.84GTKK293 pKa = 8.45RR294 pKa = 11.84TRR296 pKa = 11.84DD297 pKa = 3.24EE298 pKa = 4.15TTTIDD303 pKa = 3.71ALRR306 pKa = 11.84KK307 pKa = 9.44LKK309 pKa = 10.65LSRR312 pKa = 11.84KK313 pKa = 8.04EE314 pKa = 4.03VVRR317 pKa = 11.84EE318 pKa = 3.83PAPEE322 pKa = 3.98NPPPVGRR329 pKa = 11.84PLVPQTMGDD338 pKa = 4.78DD339 pKa = 4.02GPEE342 pKa = 3.77WAPASPEE349 pKa = 4.03RR350 pKa = 11.84TDD352 pKa = 3.4VPLVKK357 pKa = 10.16SDD359 pKa = 3.66LTSAEE364 pKa = 3.92QKK366 pKa = 10.24IALDD370 pKa = 3.45WLKK373 pKa = 10.89KK374 pKa = 10.66GEE376 pKa = 4.26APTGAMGMAQEE387 pKa = 4.25WGVVAVRR394 pKa = 11.84SDD396 pKa = 3.43LLTRR400 pKa = 11.84AEE402 pKa = 4.23VKK404 pKa = 10.31KK405 pKa = 10.77GQGGKK410 pKa = 10.46LSLSVPKK417 pKa = 10.56GATRR421 pKa = 11.84AEE423 pKa = 4.37SVALASITMMMFRR436 pKa = 11.84GFLKK440 pKa = 10.37EE441 pKa = 4.75DD442 pKa = 3.49EE443 pKa = 4.4VPYY446 pKa = 10.22LLKK449 pKa = 10.33WADD452 pKa = 3.11LWGGLMRR459 pKa = 11.84YY460 pKa = 9.8AFGLTKK466 pKa = 10.46EE467 pKa = 4.31GVEE470 pKa = 4.24LLVQEE475 pKa = 4.47AMSAGEE481 pKa = 4.2VMGEE485 pKa = 3.86

Molecular weight: 53.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1191

485

706

595.5

66.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.144 ± 1.045

0.84 ± 0.378

6.465 ± 1.15

8.564 ± 0.795

3.442 ± 1.069

8.564 ± 0.189

2.351 ± 0.91

3.694 ± 0.482

5.877 ± 1.29

8.312 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.359 ± 0.702

3.191 ± 0.304

4.366 ± 0.224

2.519 ± 0.096

7.473 ± 0.276

5.206 ± 0.153

5.458 ± 0.557

7.893 ± 0.089

2.267 ± 0.246

2.015 ± 0.218
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski