Amino acid dipepetide frequency for Bacillus virus Blastoid

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.982AlaAla: 1.982 ± 0.414
0.462AlaCys: 0.462 ± 0.187
3.171AlaAsp: 3.171 ± 0.56
3.633AlaGlu: 3.633 ± 0.525
3.765AlaPhe: 3.765 ± 0.485
4.822AlaGly: 4.822 ± 0.744
0.727AlaHis: 0.727 ± 0.207
5.087AlaIle: 5.087 ± 0.731
4.624AlaLys: 4.624 ± 0.628
5.549AlaLeu: 5.549 ± 0.849
1.718AlaMet: 1.718 ± 0.457
3.171AlaAsn: 3.171 ± 0.458
1.718AlaPro: 1.718 ± 0.411
2.048AlaGln: 2.048 ± 0.292
3.105AlaArg: 3.105 ± 0.529
3.897AlaSer: 3.897 ± 0.519
3.303AlaThr: 3.303 ± 0.518
4.822AlaVal: 4.822 ± 0.823
1.189AlaTrp: 1.189 ± 0.351
2.642AlaTyr: 2.642 ± 0.331
0.0AlaXaa: 0.0 ± 0.0
Cys
0.33CysAla: 0.33 ± 0.168
0.132CysCys: 0.132 ± 0.08
0.661CysAsp: 0.661 ± 0.202
0.396CysGlu: 0.396 ± 0.151
0.661CysPhe: 0.661 ± 0.225
0.991CysGly: 0.991 ± 0.261
0.595CysHis: 0.595 ± 0.233
0.198CysIle: 0.198 ± 0.106
1.057CysLys: 1.057 ± 0.266
0.859CysLeu: 0.859 ± 0.227
0.198CysMet: 0.198 ± 0.108
0.198CysAsn: 0.198 ± 0.108
0.33CysPro: 0.33 ± 0.13
0.264CysGln: 0.264 ± 0.154
0.396CysArg: 0.396 ± 0.176
0.661CysSer: 0.661 ± 0.189
0.595CysThr: 0.595 ± 0.226
0.198CysVal: 0.198 ± 0.111
0.066CysTrp: 0.066 ± 0.064
0.264CysTyr: 0.264 ± 0.134
0.0CysXaa: 0.0 ± 0.0
Asp
4.426AspAla: 4.426 ± 0.542
0.528AspCys: 0.528 ± 0.205
3.699AspAsp: 3.699 ± 0.54
3.633AspGlu: 3.633 ± 0.608
3.765AspPhe: 3.765 ± 0.587
4.162AspGly: 4.162 ± 0.458
1.585AspHis: 1.585 ± 0.336
4.294AspIle: 4.294 ± 0.546
4.888AspLys: 4.888 ± 0.727
5.879AspLeu: 5.879 ± 0.556
1.519AspMet: 1.519 ± 0.284
2.642AspAsn: 2.642 ± 0.456
3.039AspPro: 3.039 ± 0.411
2.048AspGln: 2.048 ± 0.297
3.567AspArg: 3.567 ± 0.416
3.039AspSer: 3.039 ± 0.426
2.642AspThr: 2.642 ± 0.394
3.831AspVal: 3.831 ± 0.396
0.727AspTrp: 0.727 ± 0.211
2.114AspTyr: 2.114 ± 0.396
0.0AspXaa: 0.0 ± 0.0
Glu
5.681GluAla: 5.681 ± 0.495
0.595GluCys: 0.595 ± 0.167
4.228GluAsp: 4.228 ± 0.583
8.456GluGlu: 8.456 ± 1.191
3.303GluPhe: 3.303 ± 0.421
4.624GluGly: 4.624 ± 0.447
1.85GluHis: 1.85 ± 0.348
4.162GluIle: 4.162 ± 0.511
5.549GluLys: 5.549 ± 0.717
8.191GluLeu: 8.191 ± 0.906
1.916GluMet: 1.916 ± 0.363
3.039GluAsn: 3.039 ± 0.506
1.784GluPro: 1.784 ± 0.424
3.964GluGln: 3.964 ± 0.699
3.501GluArg: 3.501 ± 0.471
4.096GluSer: 4.096 ± 0.528
3.699GluThr: 3.699 ± 0.421
5.351GluVal: 5.351 ± 0.633
0.925GluTrp: 0.925 ± 0.211
3.831GluTyr: 3.831 ± 0.633
0.0GluXaa: 0.0 ± 0.0
Phe
2.841PheAla: 2.841 ± 0.517
0.132PheCys: 0.132 ± 0.081
3.699PheAsp: 3.699 ± 0.563
4.03PheGlu: 4.03 ± 0.46
1.982PhePhe: 1.982 ± 0.371
2.708PheGly: 2.708 ± 0.345
0.925PheHis: 0.925 ± 0.258
2.576PheIle: 2.576 ± 0.465
3.699PheLys: 3.699 ± 0.478
3.105PheLeu: 3.105 ± 0.405
1.718PheMet: 1.718 ± 0.359
2.312PheAsn: 2.312 ± 0.296
1.585PhePro: 1.585 ± 0.316
1.189PheGln: 1.189 ± 0.228
1.651PheArg: 1.651 ± 0.321
2.51PheSer: 2.51 ± 0.425
3.633PheThr: 3.633 ± 0.517
2.444PheVal: 2.444 ± 0.374
0.396PheTrp: 0.396 ± 0.201
2.114PheTyr: 2.114 ± 0.375
0.0PheXaa: 0.0 ± 0.0
Gly
5.483GlyAla: 5.483 ± 1.011
0.925GlyCys: 0.925 ± 0.278
4.492GlyAsp: 4.492 ± 0.608
4.228GlyGlu: 4.228 ± 0.391
3.831GlyPhe: 3.831 ± 0.589
5.747GlyGly: 5.747 ± 0.925
1.453GlyHis: 1.453 ± 0.309
4.756GlyIle: 4.756 ± 0.62
5.747GlyLys: 5.747 ± 0.679
5.615GlyLeu: 5.615 ± 0.604
1.718GlyMet: 1.718 ± 0.451
3.567GlyAsn: 3.567 ± 0.79
0.132GlyPro: 0.132 ± 0.132
2.444GlyGln: 2.444 ± 0.446
2.907GlyArg: 2.907 ± 0.48
4.822GlySer: 4.822 ± 0.73
2.841GlyThr: 2.841 ± 0.396
4.228GlyVal: 4.228 ± 0.548
0.991GlyTrp: 0.991 ± 0.278
3.039GlyTyr: 3.039 ± 0.556
0.0GlyXaa: 0.0 ± 0.0
His
1.123HisAla: 1.123 ± 0.322
0.132HisCys: 0.132 ± 0.081
0.727HisAsp: 0.727 ± 0.215
1.189HisGlu: 1.189 ± 0.307
1.321HisPhe: 1.321 ± 0.308
1.85HisGly: 1.85 ± 0.433
0.661HisHis: 0.661 ± 0.189
1.519HisIle: 1.519 ± 0.279
1.651HisLys: 1.651 ± 0.403
1.982HisLeu: 1.982 ± 0.399
0.396HisMet: 0.396 ± 0.166
0.859HisAsn: 0.859 ± 0.218
1.255HisPro: 1.255 ± 0.266
0.595HisGln: 0.595 ± 0.214
1.123HisArg: 1.123 ± 0.224
1.255HisSer: 1.255 ± 0.265
1.387HisThr: 1.387 ± 0.32
1.057HisVal: 1.057 ± 0.222
0.396HisTrp: 0.396 ± 0.146
0.991HisTyr: 0.991 ± 0.287
0.0HisXaa: 0.0 ± 0.0
Ile
3.699IleAla: 3.699 ± 0.52
0.462IleCys: 0.462 ± 0.174
5.087IleAsp: 5.087 ± 0.482
5.483IleGlu: 5.483 ± 0.66
2.312IlePhe: 2.312 ± 0.53
3.964IleGly: 3.964 ± 0.615
1.321IleHis: 1.321 ± 0.24
3.964IleIle: 3.964 ± 0.454
5.945IleLys: 5.945 ± 0.718
3.964IleLeu: 3.964 ± 0.37
1.651IleMet: 1.651 ± 0.345
3.831IleAsn: 3.831 ± 0.473
2.51IlePro: 2.51 ± 0.392
2.114IleGln: 2.114 ± 0.294
3.105IleArg: 3.105 ± 0.429
3.831IleSer: 3.831 ± 0.69
4.03IleThr: 4.03 ± 0.461
4.822IleVal: 4.822 ± 0.52
0.396IleTrp: 0.396 ± 0.138
2.576IleTyr: 2.576 ± 0.471
0.0IleXaa: 0.0 ± 0.0
Lys
4.888LysAla: 4.888 ± 0.784
0.793LysCys: 0.793 ± 0.284
4.558LysAsp: 4.558 ± 0.69
8.588LysGlu: 8.588 ± 1.11
3.567LysPhe: 3.567 ± 0.573
4.756LysGly: 4.756 ± 0.776
2.51LysHis: 2.51 ± 0.415
4.294LysIle: 4.294 ± 0.493
7.266LysLys: 7.266 ± 0.884
6.936LysLeu: 6.936 ± 0.854
2.576LysMet: 2.576 ± 0.468
3.633LysAsn: 3.633 ± 0.367
3.171LysPro: 3.171 ± 0.402
4.294LysGln: 4.294 ± 0.666
3.039LysArg: 3.039 ± 0.441
4.624LysSer: 4.624 ± 0.603
3.831LysThr: 3.831 ± 0.591
4.624LysVal: 4.624 ± 0.707
0.396LysTrp: 0.396 ± 0.137
3.171LysTyr: 3.171 ± 0.435
0.0LysXaa: 0.0 ± 0.0
Leu
4.624LeuAla: 4.624 ± 0.563
0.661LeuCys: 0.661 ± 0.2
4.624LeuAsp: 4.624 ± 0.605
6.474LeuGlu: 6.474 ± 0.733
1.982LeuPhe: 1.982 ± 0.307
6.143LeuGly: 6.143 ± 0.679
1.982LeuHis: 1.982 ± 0.395
5.153LeuIle: 5.153 ± 0.671
7.729LeuLys: 7.729 ± 0.8
6.011LeuLeu: 6.011 ± 0.633
2.51LeuMet: 2.51 ± 0.443
3.964LeuAsn: 3.964 ± 0.486
3.237LeuPro: 3.237 ± 0.391
2.51LeuGln: 2.51 ± 0.442
3.699LeuArg: 3.699 ± 0.453
6.143LeuSer: 6.143 ± 0.531
5.615LeuThr: 5.615 ± 0.508
4.492LeuVal: 4.492 ± 0.538
0.991LeuTrp: 0.991 ± 0.205
2.708LeuTyr: 2.708 ± 0.496
0.0LeuXaa: 0.0 ± 0.0
Met
2.576MetAla: 2.576 ± 0.556
0.198MetCys: 0.198 ± 0.099
1.255MetAsp: 1.255 ± 0.267
1.784MetGlu: 1.784 ± 0.404
1.519MetPhe: 1.519 ± 0.293
1.585MetGly: 1.585 ± 0.402
0.793MetHis: 0.793 ± 0.255
1.321MetIle: 1.321 ± 0.256
2.51MetLys: 2.51 ± 0.393
2.378MetLeu: 2.378 ± 0.426
0.793MetMet: 0.793 ± 0.27
0.925MetAsn: 0.925 ± 0.238
0.859MetPro: 0.859 ± 0.236
0.661MetGln: 0.661 ± 0.182
1.189MetArg: 1.189 ± 0.216
3.105MetSer: 3.105 ± 0.429
1.585MetThr: 1.585 ± 0.314
1.321MetVal: 1.321 ± 0.282
0.264MetTrp: 0.264 ± 0.129
1.057MetTyr: 1.057 ± 0.353
0.0MetXaa: 0.0 ± 0.0
Asn
2.246AsnAla: 2.246 ± 0.459
0.462AsnCys: 0.462 ± 0.155
3.435AsnAsp: 3.435 ± 0.373
3.501AsnGlu: 3.501 ± 0.441
1.916AsnPhe: 1.916 ± 0.314
4.822AsnGly: 4.822 ± 0.657
0.991AsnHis: 0.991 ± 0.244
3.369AsnIle: 3.369 ± 0.523
3.699AsnLys: 3.699 ± 0.54
3.699AsnLeu: 3.699 ± 0.429
1.718AsnMet: 1.718 ± 0.349
3.105AsnAsn: 3.105 ± 0.476
1.718AsnPro: 1.718 ± 0.301
1.718AsnGln: 1.718 ± 0.379
2.246AsnArg: 2.246 ± 0.364
3.501AsnSer: 3.501 ± 0.637
2.973AsnThr: 2.973 ± 0.668
3.567AsnVal: 3.567 ± 0.436
0.925AsnTrp: 0.925 ± 0.298
1.321AsnTyr: 1.321 ± 0.301
0.0AsnXaa: 0.0 ± 0.0
Pro
2.312ProAla: 2.312 ± 0.41
0.264ProCys: 0.264 ± 0.122
2.18ProAsp: 2.18 ± 0.39
3.831ProGlu: 3.831 ± 0.625
1.85ProPhe: 1.85 ± 0.318
0.066ProGly: 0.066 ± 0.062
0.991ProHis: 0.991 ± 0.279
2.114ProIle: 2.114 ± 0.374
2.378ProLys: 2.378 ± 0.394
3.501ProLeu: 3.501 ± 0.445
0.925ProMet: 0.925 ± 0.275
1.784ProAsn: 1.784 ± 0.345
1.321ProPro: 1.321 ± 0.361
0.925ProGln: 0.925 ± 0.236
0.859ProArg: 0.859 ± 0.294
2.642ProSer: 2.642 ± 0.486
1.718ProThr: 1.718 ± 0.364
1.916ProVal: 1.916 ± 0.327
0.198ProTrp: 0.198 ± 0.088
0.793ProTyr: 0.793 ± 0.207
0.0ProXaa: 0.0 ± 0.0
Gln
2.18GlnAla: 2.18 ± 0.482
0.132GlnCys: 0.132 ± 0.077
1.85GlnAsp: 1.85 ± 0.406
3.303GlnGlu: 3.303 ± 0.491
1.718GlnPhe: 1.718 ± 0.384
2.246GlnGly: 2.246 ± 0.382
0.661GlnHis: 0.661 ± 0.26
2.642GlnIle: 2.642 ± 0.381
3.171GlnLys: 3.171 ± 0.483
2.774GlnLeu: 2.774 ± 0.441
1.057GlnMet: 1.057 ± 0.202
1.453GlnAsn: 1.453 ± 0.287
1.189GlnPro: 1.189 ± 0.23
0.925GlnGln: 0.925 ± 0.206
1.718GlnArg: 1.718 ± 0.38
2.048GlnSer: 2.048 ± 0.379
2.246GlnThr: 2.246 ± 0.481
2.444GlnVal: 2.444 ± 0.363
0.462GlnTrp: 0.462 ± 0.145
1.453GlnTyr: 1.453 ± 0.365
0.0GlnXaa: 0.0 ± 0.0
Arg
3.237ArgAla: 3.237 ± 0.452
0.396ArgCys: 0.396 ± 0.163
1.916ArgAsp: 1.916 ± 0.375
3.897ArgGlu: 3.897 ± 0.603
2.18ArgPhe: 2.18 ± 0.386
2.51ArgGly: 2.51 ± 0.477
0.793ArgHis: 0.793 ± 0.227
2.576ArgIle: 2.576 ± 0.337
4.096ArgLys: 4.096 ± 0.523
2.642ArgLeu: 2.642 ± 0.47
0.925ArgMet: 0.925 ± 0.255
3.105ArgAsn: 3.105 ± 0.536
1.123ArgPro: 1.123 ± 0.279
1.982ArgGln: 1.982 ± 0.341
2.576ArgArg: 2.576 ± 0.39
1.916ArgSer: 1.916 ± 0.389
2.708ArgThr: 2.708 ± 0.528
2.907ArgVal: 2.907 ± 0.438
0.396ArgTrp: 0.396 ± 0.175
1.784ArgTyr: 1.784 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
3.699SerAla: 3.699 ± 0.654
0.595SerCys: 0.595 ± 0.228
4.888SerAsp: 4.888 ± 0.644
4.228SerGlu: 4.228 ± 0.56
2.841SerPhe: 2.841 ± 0.346
4.954SerGly: 4.954 ± 0.877
1.321SerHis: 1.321 ± 0.249
4.822SerIle: 4.822 ± 0.613
5.417SerLys: 5.417 ± 0.551
4.954SerLeu: 4.954 ± 0.661
1.519SerMet: 1.519 ± 0.321
3.964SerAsn: 3.964 ± 0.619
1.85SerPro: 1.85 ± 0.399
2.444SerGln: 2.444 ± 0.356
2.18SerArg: 2.18 ± 0.342
4.03SerSer: 4.03 ± 0.528
3.171SerThr: 3.171 ± 0.611
3.237SerVal: 3.237 ± 0.473
0.859SerTrp: 0.859 ± 0.251
1.982SerTyr: 1.982 ± 0.359
0.0SerXaa: 0.0 ± 0.0
Thr
3.633ThrAla: 3.633 ± 0.494
0.661ThrCys: 0.661 ± 0.259
3.435ThrAsp: 3.435 ± 0.439
4.162ThrGlu: 4.162 ± 0.603
2.51ThrPhe: 2.51 ± 0.393
5.02ThrGly: 5.02 ± 0.713
0.264ThrHis: 0.264 ± 0.135
4.162ThrIle: 4.162 ± 0.472
3.237ThrLys: 3.237 ± 0.452
4.096ThrLeu: 4.096 ± 0.557
1.651ThrMet: 1.651 ± 0.362
3.567ThrAsn: 3.567 ± 0.482
3.171ThrPro: 3.171 ± 0.486
1.585ThrGln: 1.585 ± 0.31
1.916ThrArg: 1.916 ± 0.388
3.633ThrSer: 3.633 ± 0.845
3.237ThrThr: 3.237 ± 0.493
4.822ThrVal: 4.822 ± 0.534
0.727ThrTrp: 0.727 ± 0.295
2.444ThrTyr: 2.444 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
4.36ValAla: 4.36 ± 0.608
0.925ValCys: 0.925 ± 0.291
4.426ValAsp: 4.426 ± 0.552
3.831ValGlu: 3.831 ± 0.539
2.708ValPhe: 2.708 ± 0.463
3.633ValGly: 3.633 ± 0.628
1.123ValHis: 1.123 ± 0.249
4.558ValIle: 4.558 ± 0.557
5.285ValLys: 5.285 ± 0.59
4.954ValLeu: 4.954 ± 0.63
1.718ValMet: 1.718 ± 0.32
2.973ValAsn: 2.973 ± 0.454
1.916ValPro: 1.916 ± 0.313
2.378ValGln: 2.378 ± 0.435
2.576ValArg: 2.576 ± 0.485
3.567ValSer: 3.567 ± 0.53
4.888ValThr: 4.888 ± 0.675
3.964ValVal: 3.964 ± 0.564
0.595ValTrp: 0.595 ± 0.211
2.841ValTyr: 2.841 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
0.264TrpAla: 0.264 ± 0.127
0.33TrpCys: 0.33 ± 0.154
0.661TrpAsp: 0.661 ± 0.187
0.595TrpGlu: 0.595 ± 0.292
0.462TrpPhe: 0.462 ± 0.167
0.727TrpGly: 0.727 ± 0.223
0.132TrpHis: 0.132 ± 0.084
0.661TrpIle: 0.661 ± 0.19
0.727TrpLys: 0.727 ± 0.203
0.991TrpLeu: 0.991 ± 0.288
0.396TrpMet: 0.396 ± 0.146
0.925TrpAsn: 0.925 ± 0.257
0.0TrpPro: 0.0 ± 0.0
0.264TrpGln: 0.264 ± 0.163
0.661TrpArg: 0.661 ± 0.244
1.321TrpSer: 1.321 ± 0.375
0.727TrpThr: 0.727 ± 0.245
0.727TrpVal: 0.727 ± 0.286
0.198TrpTrp: 0.198 ± 0.127
0.595TrpTyr: 0.595 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.85TyrAla: 1.85 ± 0.33
0.264TyrCys: 0.264 ± 0.123
3.039TyrAsp: 3.039 ± 0.531
3.501TyrGlu: 3.501 ± 0.559
1.057TyrPhe: 1.057 ± 0.258
3.633TyrGly: 3.633 ± 0.436
0.793TyrHis: 0.793 ± 0.221
2.841TyrIle: 2.841 ± 0.42
2.841TyrLys: 2.841 ± 0.469
3.237TyrLeu: 3.237 ± 0.488
0.991TyrMet: 0.991 ± 0.255
1.916TyrAsn: 1.916 ± 0.34
0.661TyrPro: 0.661 ± 0.239
1.321TyrGln: 1.321 ± 0.317
1.651TyrArg: 1.651 ± 0.367
2.312TyrSer: 2.312 ± 0.338
3.105TyrThr: 3.105 ± 0.547
2.444TyrVal: 2.444 ± 0.519
0.264TyrTrp: 0.264 ± 0.114
1.519TyrTyr: 1.519 ± 0.344
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (15139 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski