Coleophoma cylindrospora

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Dermateaceae; Coleophoma

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3D8RH41|A0A3D8RH41_9HELO Uncharacterized protein OS=Coleophoma cylindrospora OX=1849047 GN=BP6252_07173 PE=4 SV=1
MM1 pKa = 7.42ATPTTADD8 pKa = 2.86EE9 pKa = 4.06WHH11 pKa = 6.72TLDD14 pKa = 3.81SHH16 pKa = 6.94SPTRR20 pKa = 11.84NVATCPIDD28 pKa = 4.51DD29 pKa = 4.64LNEE32 pKa = 4.29NPTSAHH38 pKa = 5.96EE39 pKa = 4.21SQPAEE44 pKa = 4.31HH45 pKa = 6.52YY46 pKa = 10.23RR47 pKa = 11.84STMSNGNGVTHH58 pKa = 7.22RR59 pKa = 11.84FIDD62 pKa = 3.45QPSPHH67 pKa = 6.32EE68 pKa = 3.77QHH70 pKa = 6.86ASSEE74 pKa = 4.47CAYY77 pKa = 10.31MMSPYY82 pKa = 10.13TDD84 pKa = 5.33DD85 pKa = 3.72ISLEE89 pKa = 4.05EE90 pKa = 4.88DD91 pKa = 3.55LGQQSDD97 pKa = 4.19EE98 pKa = 4.31EE99 pKa = 4.14AVEE102 pKa = 4.08PGEE105 pKa = 4.65PLRR108 pKa = 11.84AWLTCMFISLADD120 pKa = 3.96PNGDD124 pKa = 3.57GIMISASQSSSSSLDD139 pKa = 3.36VEE141 pKa = 4.8LSDD144 pKa = 5.56SAGQHH149 pKa = 6.62PDD151 pKa = 3.12ATDD154 pKa = 3.61DD155 pKa = 4.36SAMSDD160 pKa = 3.62LNRR163 pKa = 11.84ATDD166 pKa = 3.56TTEE169 pKa = 4.02FPLMGIGSQFGASYY183 pKa = 10.28FIEE186 pKa = 4.43EE187 pKa = 4.17

Molecular weight: 20.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3D8RN20|A0A3D8RN20_9HELO Uncharacterized protein OS=Coleophoma cylindrospora OX=1849047 GN=BP6252_06333 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 9.98CAQAILQLVCILSFLYY18 pKa = 10.73NDD20 pKa = 3.17AHH22 pKa = 6.24AQVTVYY28 pKa = 9.55LTEE31 pKa = 4.31TTTATAPPVTVTIKK45 pKa = 9.81EE46 pKa = 4.4TGSCSWPTLMSNSITPSISTSNPTRR71 pKa = 11.84SSTPSLTSRR80 pKa = 11.84PFASSTIPPNSVQSTGTSLRR100 pKa = 11.84NSNSASPTSEE110 pKa = 3.91SLTNPSSLVHH120 pKa = 5.3STGTSLQTSNSASPVSEE137 pKa = 3.97SSTNPSSPVQSTGTSLRR154 pKa = 11.84ISNSASPASGSSTNPLSPVQPTGTSLRR181 pKa = 11.84TSNLASPASGSSSNPSSLVTRR202 pKa = 11.84TSLRR206 pKa = 11.84NSKK209 pKa = 10.1SASPVSGSSTNPSSLVQSTGTGLQTSNSASPASEE243 pKa = 3.94SLSNPSIPVQSTGTSLRR260 pKa = 11.84NSNSGSPTTGGSSNPSSLVTRR281 pKa = 11.84TSLQTSNSASLVSGSSTNPSSPVQSTRR308 pKa = 11.84TSLRR312 pKa = 11.84NSNSASPASGSSTNPSSPVQSPGTSQSTGTGQSPGTSQSPGTSQSPGTSQSPGTSLRR369 pKa = 11.84TSISPTPVSGSLTGTPLTSPGQTRR393 pKa = 11.84IISTPTNPSSSRR405 pKa = 11.84GPSSPATISTISGLSSRR422 pKa = 11.84SAQPSSIVSSQPPTRR437 pKa = 11.84PLTTSSSIKK446 pKa = 9.51TSSTKK451 pKa = 10.35TIIRR455 pKa = 11.84EE456 pKa = 4.16TKK458 pKa = 9.85RR459 pKa = 11.84PQSSASTVRR468 pKa = 11.84TSLLTTQPQLSSRR481 pKa = 11.84SSQISSSVSLTPSQLSSSGTRR502 pKa = 11.84TGSLPTQSQTGSSVSSILSQLSSSGTRR529 pKa = 11.84TGSIPTQSQTGSSVSSILSQLSSSGTRR556 pKa = 11.84TGSLPTQSQTGSSVSPILSQISSSGTRR583 pKa = 11.84TGSLPTQSQTGSSVSPISSQILRR606 pKa = 11.84SSVGTSSLPLQSQASSRR623 pKa = 11.84LSQTASSVPSTLSQISSDD641 pKa = 3.38TSTGSLPTQSQTGASASSILPQPSSSSIGTSSLPLQSQASSRR683 pKa = 11.84LAQTASSASLSTSGLQATSLPTGSQFSSGATSRR716 pKa = 11.84STAAAASSQSLISISSSAAQSNSLQATSSLTEE748 pKa = 4.4SPTSSPFTISPEE760 pKa = 4.17DD761 pKa = 3.4ASGASMSRR769 pKa = 11.84FSLSRR774 pKa = 11.84PGTSQTSAAPSGASTIPSSIGVSTLSSGLPSTTGSSIISMQGTSQSSSLNTQSPSRR830 pKa = 11.84ISTSEE835 pKa = 3.84TSILTPTAPGLSSTVTPALSQSSKK859 pKa = 10.76LGSTSSVSSAGGRR872 pKa = 11.84AGALGAVRR880 pKa = 11.84PGVVGATISRR890 pKa = 11.84ASTSPTTTLPTTTGSSGPSNSGSSLSSVPILSQPPSSIPMTATSNLLSPSTTLISTPSNSPLATPSTPLSSIPSIRR966 pKa = 11.84QSSTPGTGLSSVTIPGQSTTPGTLSPEE993 pKa = 4.19LSTTPGTPLSSTPIPGQSTTPGTPLSSALISGQSTTPGTPLSSALISGQSTTPGKK1048 pKa = 9.3PLSSTPIPGQSSTPGVTTPGLSTTPGTRR1076 pKa = 11.84PASTPIPGQSTIPGTPSPGQSTTHH1100 pKa = 5.73GTPNPGQPTTPGTPTPGQSTTPGNPTPGLSTTLGTASPGQSSTPGTQSPGMFVTPGTRR1158 pKa = 11.84PASTPVPGQSTTQGTPNPGQSTEE1181 pKa = 4.46PGTPSPGLSSSPSTPNSGQSTTPGTILASNPIPGQSTTQGTTLSSTPISGQSTTSGTPLFSTPILGQSSTPGAPSPGLSTTPGTRR1266 pKa = 11.84PASTPIPGQSTAPGTTNPGQSTTPGTPLSSTPIPGQSSAPGASSPGLSTTIPGQSSTPGAPSPGLSSTPGTLNPGQSTAPGTTNPGQSTTPGRR1359 pKa = 11.84PLSSTPIPGQSSTPGAPSPGLSTTPGTRR1387 pKa = 11.84LASTPTPGQSTTPGTPSSGQSTTLGTQLSSTPTPGQSTIPGTTLSSVPSASRR1439 pKa = 11.84STTSGAALTSTPGTAEE1455 pKa = 4.35LSTPSQSSLLPSGASPSLQASPSAISTSSVQSASASASTLSGASTPLSGSTGSSHH1510 pKa = 5.55STSVQPATSVSSFLTTFKK1528 pKa = 11.02SSTTSSPSATSTCVDD1543 pKa = 3.2RR1544 pKa = 11.84LVVNGGFEE1552 pKa = 4.21TGALSPWSQPVTLEE1566 pKa = 3.62VSAFFGPINSPTLCRR1581 pKa = 11.84TGQYY1585 pKa = 10.82CLGNGLYY1592 pKa = 10.42DD1593 pKa = 3.93GSNIMFKK1600 pKa = 10.57QSVQLCSNEE1609 pKa = 4.1SFRR1612 pKa = 11.84ASAWARR1618 pKa = 11.84ADD1620 pKa = 3.51TGDD1623 pKa = 3.71SVCYY1627 pKa = 10.08FEE1629 pKa = 6.37VCIQSPKK1636 pKa = 9.31HH1637 pKa = 5.8CSGPIIVDD1645 pKa = 3.8TTWTEE1650 pKa = 3.57ASFTFSGAPFSPKK1663 pKa = 10.2ADD1665 pKa = 3.37LEE1667 pKa = 4.69VYY1669 pKa = 8.81TGCIGDD1675 pKa = 4.44DD1676 pKa = 3.48STSFEE1681 pKa = 4.55IFIDD1685 pKa = 4.69DD1686 pKa = 4.27IAIQQVV1692 pKa = 2.92

Molecular weight: 165.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14176

0

14176

7204061

49

14673

508.2

56.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.66 ± 0.019

1.262 ± 0.009

5.448 ± 0.015

6.022 ± 0.021

3.846 ± 0.011

6.945 ± 0.02

2.252 ± 0.009

5.326 ± 0.016

4.875 ± 0.018

8.961 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.235 ± 0.008

3.818 ± 0.011

5.69 ± 0.019

3.927 ± 0.013

5.458 ± 0.019

8.459 ± 0.031

6.258 ± 0.023

6.151 ± 0.014

1.513 ± 0.008

2.896 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski