Amino acid dipepetide frequency for Bacillus phage CampHawk

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.962AlaAla: 1.962 ± 0.258
0.535AlaCys: 0.535 ± 0.103
3.262AlaAsp: 3.262 ± 0.317
3.899AlaGlu: 3.899 ± 0.296
2.396AlaPhe: 2.396 ± 0.275
3.695AlaGly: 3.695 ± 0.359
0.79AlaHis: 0.79 ± 0.128
3.848AlaIle: 3.848 ± 0.302
4.867AlaLys: 4.867 ± 0.322
5.122AlaLeu: 5.122 ± 0.361
1.58AlaMet: 1.58 ± 0.23
2.625AlaAsn: 2.625 ± 0.294
1.835AlaPro: 1.835 ± 0.256
1.962AlaGln: 1.962 ± 0.26
2.497AlaArg: 2.497 ± 0.258
3.007AlaSer: 3.007 ± 0.301
2.829AlaThr: 2.829 ± 0.344
4.358AlaVal: 4.358 ± 0.356
0.561AlaTrp: 0.561 ± 0.103
2.345AlaTyr: 2.345 ± 0.24
0.0AlaXaa: 0.0 ± 0.0
Cys
0.535CysAla: 0.535 ± 0.123
0.076CysCys: 0.076 ± 0.043
0.408CysAsp: 0.408 ± 0.122
0.841CysGlu: 0.841 ± 0.161
0.229CysPhe: 0.229 ± 0.069
0.637CysGly: 0.637 ± 0.146
0.229CysHis: 0.229 ± 0.079
0.561CysIle: 0.561 ± 0.116
1.045CysLys: 1.045 ± 0.166
0.663CysLeu: 0.663 ± 0.149
0.255CysMet: 0.255 ± 0.073
0.484CysAsn: 0.484 ± 0.126
0.306CysPro: 0.306 ± 0.109
0.484CysGln: 0.484 ± 0.104
0.408CysArg: 0.408 ± 0.102
0.714CysSer: 0.714 ± 0.121
0.688CysThr: 0.688 ± 0.176
0.637CysVal: 0.637 ± 0.129
0.127CysTrp: 0.127 ± 0.055
0.484CysTyr: 0.484 ± 0.086
0.0CysXaa: 0.0 ± 0.0
Asp
3.338AspAla: 3.338 ± 0.293
0.866AspCys: 0.866 ± 0.144
3.899AspAsp: 3.899 ± 0.337
4.434AspGlu: 4.434 ± 0.454
2.676AspPhe: 2.676 ± 0.261
4.511AspGly: 4.511 ± 0.391
0.892AspHis: 0.892 ± 0.157
4.969AspIle: 4.969 ± 0.434
4.995AspLys: 4.995 ± 0.39
7.059AspLeu: 7.059 ± 0.522
2.013AspMet: 2.013 ± 0.241
3.721AspAsn: 3.721 ± 0.331
2.676AspPro: 2.676 ± 0.286
2.064AspGln: 2.064 ± 0.224
2.88AspArg: 2.88 ± 0.265
4.154AspSer: 4.154 ± 0.311
4.307AspThr: 4.307 ± 0.316
4.383AspVal: 4.383 ± 0.337
0.739AspTrp: 0.739 ± 0.134
2.523AspTyr: 2.523 ± 0.287
0.0AspXaa: 0.0 ± 0.0
Glu
4.358GluAla: 4.358 ± 0.333
0.688GluCys: 0.688 ± 0.12
7.288GluAsp: 7.288 ± 0.577
8.384GluGlu: 8.384 ± 0.831
2.956GluPhe: 2.956 ± 0.247
6.167GluGly: 6.167 ± 0.397
1.376GluHis: 1.376 ± 0.19
4.842GluIle: 4.842 ± 0.36
6.779GluLys: 6.779 ± 0.562
6.346GluLeu: 6.346 ± 0.489
2.141GluMet: 2.141 ± 0.216
3.644GluAsn: 3.644 ± 0.289
2.217GluPro: 2.217 ± 0.259
2.625GluGln: 2.625 ± 0.284
3.109GluArg: 3.109 ± 0.311
4.23GluSer: 4.23 ± 0.364
3.236GluThr: 3.236 ± 0.311
6.014GluVal: 6.014 ± 0.443
1.045GluTrp: 1.045 ± 0.133
3.313GluTyr: 3.313 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
1.478PheAla: 1.478 ± 0.187
0.204PheCys: 0.204 ± 0.069
2.294PheAsp: 2.294 ± 0.214
2.727PheGlu: 2.727 ± 0.249
1.172PhePhe: 1.172 ± 0.177
2.192PheGly: 2.192 ± 0.32
0.841PheHis: 0.841 ± 0.142
2.166PheIle: 2.166 ± 0.237
2.905PheLys: 2.905 ± 0.338
3.033PheLeu: 3.033 ± 0.277
1.249PheMet: 1.249 ± 0.198
1.962PheAsn: 1.962 ± 0.208
1.147PhePro: 1.147 ± 0.202
0.994PheGln: 0.994 ± 0.176
1.988PheArg: 1.988 ± 0.188
2.625PheSer: 2.625 ± 0.286
2.217PheThr: 2.217 ± 0.279
2.09PheVal: 2.09 ± 0.202
0.408PheTrp: 0.408 ± 0.108
1.453PheTyr: 1.453 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
3.925GlyAla: 3.925 ± 0.433
0.79GlyCys: 0.79 ± 0.143
4.409GlyAsp: 4.409 ± 0.392
5.25GlyGlu: 5.25 ± 0.344
2.446GlyPhe: 2.446 ± 0.273
4.867GlyGly: 4.867 ± 0.523
0.79GlyHis: 0.79 ± 0.147
4.689GlyIle: 4.689 ± 0.444
4.613GlyLys: 4.613 ± 0.342
5.479GlyLeu: 5.479 ± 0.426
1.758GlyMet: 1.758 ± 0.237
3.033GlyAsn: 3.033 ± 0.398
1.019GlyPro: 1.019 ± 0.193
1.784GlyGln: 1.784 ± 0.205
3.033GlyArg: 3.033 ± 0.301
4.562GlySer: 4.562 ± 0.388
4.409GlyThr: 4.409 ± 0.338
5.938GlyVal: 5.938 ± 0.455
0.79GlyTrp: 0.79 ± 0.135
3.695GlyTyr: 3.695 ± 0.319
0.0GlyXaa: 0.0 ± 0.0
His
0.815HisAla: 0.815 ± 0.156
0.178HisCys: 0.178 ± 0.061
1.121HisAsp: 1.121 ± 0.174
0.994HisGlu: 0.994 ± 0.181
0.688HisPhe: 0.688 ± 0.113
1.223HisGly: 1.223 ± 0.204
0.484HisHis: 0.484 ± 0.102
1.274HisIle: 1.274 ± 0.174
1.631HisLys: 1.631 ± 0.259
1.478HisLeu: 1.478 ± 0.194
0.561HisMet: 0.561 ± 0.109
0.841HisAsn: 0.841 ± 0.137
0.841HisPro: 0.841 ± 0.153
0.306HisGln: 0.306 ± 0.087
0.994HisArg: 0.994 ± 0.15
1.249HisSer: 1.249 ± 0.173
1.223HisThr: 1.223 ± 0.172
1.325HisVal: 1.325 ± 0.17
0.331HisTrp: 0.331 ± 0.095
0.994HisTyr: 0.994 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
3.389IleAla: 3.389 ± 0.305
0.612IleCys: 0.612 ± 0.129
4.613IleAsp: 4.613 ± 0.323
5.454IleGlu: 5.454 ± 0.405
1.809IlePhe: 1.809 ± 0.248
4.103IleGly: 4.103 ± 0.32
1.453IleHis: 1.453 ± 0.192
4.205IleIle: 4.205 ± 0.435
5.759IleLys: 5.759 ± 0.308
4.867IleLeu: 4.867 ± 0.405
1.555IleMet: 1.555 ± 0.2
3.517IleAsn: 3.517 ± 0.276
2.548IlePro: 2.548 ± 0.246
2.396IleGln: 2.396 ± 0.25
2.931IleArg: 2.931 ± 0.288
3.721IleSer: 3.721 ± 0.448
4.154IleThr: 4.154 ± 0.369
3.95IleVal: 3.95 ± 0.292
0.306IleTrp: 0.306 ± 0.084
2.37IleTyr: 2.37 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
5.148LysAla: 5.148 ± 0.431
0.612LysCys: 0.612 ± 0.143
5.759LysAsp: 5.759 ± 0.499
7.441LysGlu: 7.441 ± 0.609
2.446LysPhe: 2.446 ± 0.255
5.25LysGly: 5.25 ± 0.37
1.937LysHis: 1.937 ± 0.271
4.23LysIle: 4.23 ± 0.318
7.773LysLys: 7.773 ± 0.634
5.505LysLeu: 5.505 ± 0.379
2.472LysMet: 2.472 ± 0.228
3.542LysAsn: 3.542 ± 0.291
2.905LysPro: 2.905 ± 0.281
2.497LysGln: 2.497 ± 0.29
3.848LysArg: 3.848 ± 0.337
5.199LysSer: 5.199 ± 0.442
3.874LysThr: 3.874 ± 0.278
5.912LysVal: 5.912 ± 0.419
1.045LysTrp: 1.045 ± 0.169
3.899LysTyr: 3.899 ± 0.321
0.0LysXaa: 0.0 ± 0.0
Leu
4.511LeuAla: 4.511 ± 0.403
0.815LeuCys: 0.815 ± 0.156
6.142LeuAsp: 6.142 ± 0.417
8.053LeuGlu: 8.053 ± 0.531
2.88LeuPhe: 2.88 ± 0.214
5.097LeuGly: 5.097 ± 0.331
1.427LeuHis: 1.427 ± 0.174
4.23LeuIle: 4.23 ± 0.358
6.269LeuLys: 6.269 ± 0.459
6.269LeuLeu: 6.269 ± 0.508
2.115LeuMet: 2.115 ± 0.237
3.593LeuAsn: 3.593 ± 0.294
2.88LeuPro: 2.88 ± 0.3
3.364LeuGln: 3.364 ± 0.335
3.95LeuArg: 3.95 ± 0.326
6.065LeuSer: 6.065 ± 0.431
4.536LeuThr: 4.536 ± 0.336
4.867LeuVal: 4.867 ± 0.33
0.943LeuTrp: 0.943 ± 0.143
3.16LeuTyr: 3.16 ± 0.294
0.0LeuXaa: 0.0 ± 0.0
Met
2.217MetAla: 2.217 ± 0.251
0.255MetCys: 0.255 ± 0.083
2.115MetAsp: 2.115 ± 0.236
2.319MetGlu: 2.319 ± 0.291
0.765MetPhe: 0.765 ± 0.14
1.656MetGly: 1.656 ± 0.236
0.459MetHis: 0.459 ± 0.092
1.504MetIle: 1.504 ± 0.186
2.345MetLys: 2.345 ± 0.268
1.86MetLeu: 1.86 ± 0.197
0.688MetMet: 0.688 ± 0.117
1.376MetAsn: 1.376 ± 0.22
0.815MetPro: 0.815 ± 0.132
0.815MetGln: 0.815 ± 0.14
1.172MetArg: 1.172 ± 0.169
2.013MetSer: 2.013 ± 0.182
1.529MetThr: 1.529 ± 0.204
1.809MetVal: 1.809 ± 0.236
0.331MetTrp: 0.331 ± 0.082
1.172MetTyr: 1.172 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
2.37AsnAla: 2.37 ± 0.263
0.663AsnCys: 0.663 ± 0.158
2.319AsnAsp: 2.319 ± 0.272
2.752AsnGlu: 2.752 ± 0.259
1.962AsnPhe: 1.962 ± 0.234
3.695AsnGly: 3.695 ± 0.363
0.994AsnHis: 0.994 ± 0.191
3.593AsnIle: 3.593 ± 0.304
4.205AsnLys: 4.205 ± 0.309
3.95AsnLeu: 3.95 ± 0.313
1.223AsnMet: 1.223 ± 0.193
2.982AsnAsn: 2.982 ± 0.386
2.37AsnPro: 2.37 ± 0.245
1.555AsnGln: 1.555 ± 0.219
2.294AsnArg: 2.294 ± 0.264
3.415AsnSer: 3.415 ± 0.253
3.619AsnThr: 3.619 ± 0.514
3.211AsnVal: 3.211 ± 0.339
0.51AsnTrp: 0.51 ± 0.103
1.809AsnTyr: 1.809 ± 0.2
0.0AsnXaa: 0.0 ± 0.0
Pro
1.529ProAla: 1.529 ± 0.215
0.306ProCys: 0.306 ± 0.087
2.446ProAsp: 2.446 ± 0.255
3.186ProGlu: 3.186 ± 0.235
1.325ProPhe: 1.325 ± 0.195
1.758ProGly: 1.758 ± 0.163
0.433ProHis: 0.433 ± 0.093
2.141ProIle: 2.141 ± 0.197
2.905ProLys: 2.905 ± 0.295
2.243ProLeu: 2.243 ± 0.243
0.637ProMet: 0.637 ± 0.13
1.606ProAsn: 1.606 ± 0.231
0.943ProPro: 0.943 ± 0.172
1.019ProGln: 1.019 ± 0.179
1.402ProArg: 1.402 ± 0.189
2.421ProSer: 2.421 ± 0.26
2.472ProThr: 2.472 ± 0.244
2.778ProVal: 2.778 ± 0.292
0.331ProTrp: 0.331 ± 0.095
1.656ProTyr: 1.656 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
1.988GlnAla: 1.988 ± 0.23
0.357GlnCys: 0.357 ± 0.089
1.988GlnAsp: 1.988 ± 0.18
2.523GlnGlu: 2.523 ± 0.373
0.943GlnPhe: 0.943 ± 0.201
1.937GlnGly: 1.937 ± 0.195
0.765GlnHis: 0.765 ± 0.152
2.243GlnIle: 2.243 ± 0.24
2.446GlnLys: 2.446 ± 0.259
3.135GlnLeu: 3.135 ± 0.255
0.994GlnMet: 0.994 ± 0.147
1.453GlnAsn: 1.453 ± 0.202
0.866GlnPro: 0.866 ± 0.131
1.121GlnGln: 1.121 ± 0.21
1.504GlnArg: 1.504 ± 0.186
2.115GlnSer: 2.115 ± 0.219
1.886GlnThr: 1.886 ± 0.25
2.548GlnVal: 2.548 ± 0.208
0.408GlnTrp: 0.408 ± 0.095
1.962GlnTyr: 1.962 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
2.396ArgAla: 2.396 ± 0.259
0.408ArgCys: 0.408 ± 0.099
2.625ArgAsp: 2.625 ± 0.237
3.058ArgGlu: 3.058 ± 0.299
1.682ArgPhe: 1.682 ± 0.21
2.956ArgGly: 2.956 ± 0.284
0.612ArgHis: 0.612 ± 0.157
3.109ArgIle: 3.109 ± 0.222
4.128ArgLys: 4.128 ± 0.319
3.899ArgLeu: 3.899 ± 0.325
1.606ArgMet: 1.606 ± 0.245
2.497ArgAsn: 2.497 ± 0.233
1.376ArgPro: 1.376 ± 0.203
1.835ArgGln: 1.835 ± 0.194
2.676ArgArg: 2.676 ± 0.237
2.65ArgSer: 2.65 ± 0.294
2.396ArgThr: 2.396 ± 0.206
3.542ArgVal: 3.542 ± 0.274
0.637ArgTrp: 0.637 ± 0.138
1.682ArgTyr: 1.682 ± 0.201
0.0ArgXaa: 0.0 ± 0.0
Ser
3.287SerAla: 3.287 ± 0.295
0.306SerCys: 0.306 ± 0.097
4.154SerAsp: 4.154 ± 0.331
4.715SerGlu: 4.715 ± 0.375
2.497SerPhe: 2.497 ± 0.262
4.715SerGly: 4.715 ± 0.363
1.121SerHis: 1.121 ± 0.163
4.154SerIle: 4.154 ± 0.299
5.25SerLys: 5.25 ± 0.399
5.377SerLeu: 5.377 ± 0.456
1.86SerMet: 1.86 ± 0.225
3.16SerAsn: 3.16 ± 0.324
1.962SerPro: 1.962 ± 0.243
2.166SerGln: 2.166 ± 0.298
3.364SerArg: 3.364 ± 0.31
3.848SerSer: 3.848 ± 0.419
4.307SerThr: 4.307 ± 0.355
4.791SerVal: 4.791 ± 0.412
0.688SerTrp: 0.688 ± 0.141
3.007SerTyr: 3.007 ± 0.29
0.0SerXaa: 0.0 ± 0.0
Thr
4.027ThrAla: 4.027 ± 0.358
0.586ThrCys: 0.586 ± 0.12
3.874ThrAsp: 3.874 ± 0.299
4.74ThrGlu: 4.74 ± 0.381
2.421ThrPhe: 2.421 ± 0.233
4.817ThrGly: 4.817 ± 0.435
1.172ThrHis: 1.172 ± 0.2
4.307ThrIle: 4.307 ± 0.338
4.103ThrLys: 4.103 ± 0.393
4.409ThrLeu: 4.409 ± 0.334
1.147ThrMet: 1.147 ± 0.161
2.803ThrAsn: 2.803 ± 0.305
2.548ThrPro: 2.548 ± 0.269
2.192ThrGln: 2.192 ± 0.264
2.039ThrArg: 2.039 ± 0.276
3.593ThrSer: 3.593 ± 0.378
3.466ThrThr: 3.466 ± 0.353
4.358ThrVal: 4.358 ± 0.406
0.765ThrTrp: 0.765 ± 0.138
2.65ThrTyr: 2.65 ± 0.266
0.0ThrXaa: 0.0 ± 0.0
Val
4.307ValAla: 4.307 ± 0.346
0.765ValCys: 0.765 ± 0.125
4.791ValAsp: 4.791 ± 0.407
6.04ValGlu: 6.04 ± 0.44
1.707ValPhe: 1.707 ± 0.189
4.307ValGly: 4.307 ± 0.347
1.478ValHis: 1.478 ± 0.183
4.128ValIle: 4.128 ± 0.337
5.352ValLys: 5.352 ± 0.395
5.632ValLeu: 5.632 ± 0.326
1.682ValMet: 1.682 ± 0.18
3.262ValAsn: 3.262 ± 0.272
2.905ValPro: 2.905 ± 0.299
2.421ValGln: 2.421 ± 0.208
3.058ValArg: 3.058 ± 0.346
5.632ValSer: 5.632 ± 0.406
5.657ValThr: 5.657 ± 0.462
5.836ValVal: 5.836 ± 0.47
0.535ValTrp: 0.535 ± 0.108
3.236ValTyr: 3.236 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
0.637TrpAla: 0.637 ± 0.123
0.306TrpCys: 0.306 ± 0.095
0.815TrpAsp: 0.815 ± 0.152
0.968TrpGlu: 0.968 ± 0.155
0.459TrpPhe: 0.459 ± 0.121
0.968TrpGly: 0.968 ± 0.142
0.255TrpHis: 0.255 ± 0.09
0.637TrpIle: 0.637 ± 0.119
0.714TrpLys: 0.714 ± 0.12
0.943TrpLeu: 0.943 ± 0.148
0.382TrpMet: 0.382 ± 0.104
0.637TrpAsn: 0.637 ± 0.124
0.0TrpPro: 0.0 ± 0.0
0.306TrpGln: 0.306 ± 0.091
0.459TrpArg: 0.459 ± 0.091
0.612TrpSer: 0.612 ± 0.128
0.306TrpThr: 0.306 ± 0.081
1.121TrpVal: 1.121 ± 0.152
0.255TrpTrp: 0.255 ± 0.079
0.357TrpTyr: 0.357 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.758TyrAla: 1.758 ± 0.236
0.484TyrCys: 0.484 ± 0.094
2.778TyrAsp: 2.778 ± 0.252
2.982TyrGlu: 2.982 ± 0.269
1.682TyrPhe: 1.682 ± 0.205
2.727TyrGly: 2.727 ± 0.272
1.045TyrHis: 1.045 ± 0.149
2.88TyrIle: 2.88 ± 0.266
3.211TyrLys: 3.211 ± 0.282
3.899TyrLeu: 3.899 ± 0.298
1.274TyrMet: 1.274 ± 0.193
2.752TyrAsn: 2.752 ± 0.234
1.376TyrPro: 1.376 ± 0.195
1.274TyrGln: 1.274 ± 0.162
2.166TyrArg: 2.166 ± 0.266
2.905TyrSer: 2.905 ± 0.262
2.905TyrThr: 2.905 ± 0.243
3.262TyrVal: 3.262 ± 0.34
0.382TyrTrp: 0.382 ± 0.098
1.656TyrTyr: 1.656 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 201 proteins (39241 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski