Sinorhizobium phage phi2LM21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291AUJ0|A0A291AUJ0_9CAUD Uncharacterized protein OS=Sinorhizobium phage phi2LM21 OX=2029632 GN=phi2LM21_p06 PE=4 SV=1
MM1 pKa = 7.94ADD3 pKa = 3.76LFGNSQMEE11 pKa = 4.76AATKK15 pKa = 8.85WCPTVWLDD23 pKa = 3.78EE24 pKa = 4.24VAGILVADD32 pKa = 4.72PADD35 pKa = 4.06CVHH38 pKa = 6.97GDD40 pKa = 3.84GNGGWILLDD49 pKa = 3.52EE50 pKa = 4.79DD51 pKa = 4.8AEE53 pKa = 4.16NDD55 pKa = 4.17RR56 pKa = 11.84LDD58 pKa = 4.1GCCMQVHH65 pKa = 7.36PGQIVSFCANRR76 pKa = 11.84MYY78 pKa = 10.88GYY80 pKa = 10.72FEE82 pKa = 6.11LIVYY86 pKa = 10.0DD87 pKa = 5.16DD88 pKa = 3.9RR89 pKa = 11.84TFVCAPYY96 pKa = 9.78PADD99 pKa = 3.44ATHH102 pKa = 6.85FAHH105 pKa = 7.46AEE107 pKa = 4.01EE108 pKa = 5.16ADD110 pKa = 3.81SEE112 pKa = 4.99TMFDD116 pKa = 3.94SVAEE120 pKa = 4.02LVAQGDD126 pKa = 4.11PEE128 pKa = 4.75GFGSAPLPAGPHH140 pKa = 7.29LINIWHH146 pKa = 7.02WSDD149 pKa = 3.92EE150 pKa = 3.85IAFRR154 pKa = 11.84FEE156 pKa = 4.31VEE158 pKa = 3.83DD159 pKa = 3.86GKK161 pKa = 10.31PKK163 pKa = 10.45FVRR166 pKa = 11.84CAGANN171 pKa = 3.33

Molecular weight: 18.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291AUL2|A0A291AUL2_9CAUD Putative C-5 cytosine-specific DNA methylase OS=Sinorhizobium phage phi2LM21 OX=2029632 GN=phi2LM21_p23 PE=4 SV=1
MM1 pKa = 7.63IDD3 pKa = 3.09AHH5 pKa = 7.2IKK7 pKa = 9.96FDD9 pKa = 3.71LSGFEE14 pKa = 3.66RR15 pKa = 11.84SLRR18 pKa = 11.84DD19 pKa = 3.26IEE21 pKa = 5.23RR22 pKa = 11.84KK23 pKa = 8.37QLPYY27 pKa = 10.81AIILTLNEE35 pKa = 3.92TAKK38 pKa = 10.65GGRR41 pKa = 11.84LEE43 pKa = 4.32VQRR46 pKa = 11.84EE47 pKa = 3.75MDD49 pKa = 3.39RR50 pKa = 11.84VFDD53 pKa = 4.68RR54 pKa = 11.84PTPYY58 pKa = 10.78AKK60 pKa = 9.96RR61 pKa = 11.84GVVFDD66 pKa = 3.66RR67 pKa = 11.84ATRR70 pKa = 11.84QNLQAAVVVTGDD82 pKa = 3.45RR83 pKa = 11.84TKK85 pKa = 11.26GGLPATAFLGPQIEE99 pKa = 4.61GGMRR103 pKa = 11.84SHH105 pKa = 7.5KK106 pKa = 10.62AFEE109 pKa = 4.08RR110 pKa = 11.84QLIDD114 pKa = 3.48RR115 pKa = 11.84NLMKK119 pKa = 10.46RR120 pKa = 11.84NEE122 pKa = 4.16VAVPAKK128 pKa = 10.1RR129 pKa = 11.84APLDD133 pKa = 3.27RR134 pKa = 11.84YY135 pKa = 11.39GNMTQGFLNRR145 pKa = 11.84VMADD149 pKa = 3.11LQIDD153 pKa = 3.86YY154 pKa = 10.08RR155 pKa = 11.84GAGATRR161 pKa = 11.84VRR163 pKa = 11.84SDD165 pKa = 3.66KK166 pKa = 10.82SVKK169 pKa = 9.97RR170 pKa = 11.84NKK172 pKa = 9.74NYY174 pKa = 10.35KK175 pKa = 8.61NARR178 pKa = 11.84FFAAKK183 pKa = 10.03RR184 pKa = 11.84PGHH187 pKa = 7.21LYY189 pKa = 9.77PGVWQRR195 pKa = 11.84DD196 pKa = 3.67PTTQAIFPVILFVSQSSYY214 pKa = 10.43RR215 pKa = 11.84VRR217 pKa = 11.84LKK219 pKa = 10.61LRR221 pKa = 11.84EE222 pKa = 4.04VVEE225 pKa = 4.77SYY227 pKa = 8.55VTANIHH233 pKa = 5.69DD234 pKa = 4.03HH235 pKa = 5.97FAAAFEE241 pKa = 4.33RR242 pKa = 11.84AVRR245 pKa = 11.84TARR248 pKa = 3.47

Molecular weight: 28.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14586

36

876

211.4

23.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.333 ± 0.401

1.118 ± 0.124

6.239 ± 0.17

6.506 ± 0.318

3.736 ± 0.208

8.248 ± 0.303

1.817 ± 0.152

5.053 ± 0.158

4.607 ± 0.256

8.138 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.153

3.236 ± 0.227

4.984 ± 0.247

3.709 ± 0.2

7.398 ± 0.254

5.629 ± 0.182

5.073 ± 0.212

6.63 ± 0.226

1.666 ± 0.166

2.509 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski