Psychrobacillus insolitus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W7MGV4|A0A2W7MGV4_9BACI Uncharacterized protein OS=Psychrobacillus insolitus OX=1461 GN=C7437_10372 PE=4 SV=1
MM1 pKa = 7.34SASKK5 pKa = 10.72NSSKK9 pKa = 10.92NFLITGVLVCLFTLGGIGYY28 pKa = 8.53GFKK31 pKa = 10.72EE32 pKa = 4.2SFFAKK37 pKa = 10.49DD38 pKa = 2.94EE39 pKa = 4.91AIPVIEE45 pKa = 4.58EE46 pKa = 4.14LKK48 pKa = 9.87TLNKK52 pKa = 8.86QAPVAEE58 pKa = 4.34IEE60 pKa = 4.43TKK62 pKa = 10.38KK63 pKa = 10.87VAIQQKK69 pKa = 10.46DD70 pKa = 3.26KK71 pKa = 11.04TKK73 pKa = 10.58VIQEE77 pKa = 4.12TMPKK81 pKa = 9.81VFTILTEE88 pKa = 4.07VGQGSGFLYY97 pKa = 10.3EE98 pKa = 3.82QGGYY102 pKa = 9.7IVTNAHH108 pKa = 5.02VVAGYY113 pKa = 9.24TDD115 pKa = 3.65VIVRR119 pKa = 11.84NSAGKK124 pKa = 10.06DD125 pKa = 3.14SSAKK129 pKa = 10.5VIGISDD135 pKa = 3.73EE136 pKa = 4.32SDD138 pKa = 3.16VALLISAEE146 pKa = 4.1YY147 pKa = 10.92AKK149 pKa = 10.81VNPLEE154 pKa = 4.39TEE156 pKa = 4.07LEE158 pKa = 4.03EE159 pKa = 5.08SIVGTEE165 pKa = 4.17VIALGSPQGFEE176 pKa = 3.85NSASIGYY183 pKa = 8.03LTGVNRR189 pKa = 11.84DD190 pKa = 3.0IEE192 pKa = 4.54FGFLYY197 pKa = 10.29EE198 pKa = 4.64HH199 pKa = 7.69LYY201 pKa = 10.81QIDD204 pKa = 3.69AQIDD208 pKa = 3.73EE209 pKa = 4.88GSSGGPLIDD218 pKa = 3.89AKK220 pKa = 9.45TGKK223 pKa = 10.66VIGINSLLFKK233 pKa = 10.6EE234 pKa = 4.02NDD236 pKa = 3.24VFGFSIPMNTVTSILDD252 pKa = 3.41NWISSPMNEE261 pKa = 4.21KK262 pKa = 10.31QVTSLFGVYY271 pKa = 9.79EE272 pKa = 4.79DD273 pKa = 3.83YY274 pKa = 10.66TYY276 pKa = 11.56SDD278 pKa = 4.06EE279 pKa = 4.59NVSEE283 pKa = 3.96DD284 pKa = 3.32TYY286 pKa = 11.42YY287 pKa = 10.97EE288 pKa = 3.72EE289 pKa = 5.03DD290 pKa = 3.64AYY292 pKa = 11.26SEE294 pKa = 4.09EE295 pKa = 4.94SIPEE299 pKa = 4.07DD300 pKa = 3.47TSDD303 pKa = 4.57EE304 pKa = 3.92EE305 pKa = 4.72DD306 pKa = 3.58ANSDD310 pKa = 3.64EE311 pKa = 4.1IASYY315 pKa = 10.78EE316 pKa = 4.12EE317 pKa = 4.38EE318 pKa = 4.5SVEE321 pKa = 5.75DD322 pKa = 3.68DD323 pKa = 3.32WNDD326 pKa = 3.04NSFEE330 pKa = 4.18EE331 pKa = 4.52EE332 pKa = 4.16SLSNFIISFRR342 pKa = 11.84GYY344 pKa = 11.11YY345 pKa = 10.25EE346 pKa = 3.79MALADD351 pKa = 4.95EE352 pKa = 4.77DD353 pKa = 4.78FSWIEE358 pKa = 4.06DD359 pKa = 3.49MLGPGSIAYY368 pKa = 9.13IDD370 pKa = 3.89LEE372 pKa = 4.43KK373 pKa = 10.93YY374 pKa = 10.53VYY376 pKa = 10.11DD377 pKa = 3.55VSGQGVIFDD386 pKa = 4.22FTSNTVTAVEE396 pKa = 4.36IFDD399 pKa = 5.49DD400 pKa = 4.08YY401 pKa = 12.14ALVSTNEE408 pKa = 3.93EE409 pKa = 3.73FDD411 pKa = 3.56FTNAAGEE418 pKa = 4.33YY419 pKa = 9.94EE420 pKa = 4.49SYY422 pKa = 11.27NRR424 pKa = 11.84DD425 pKa = 2.9KK426 pKa = 10.65EE427 pKa = 4.32YY428 pKa = 10.57TVMIDD433 pKa = 3.97SEE435 pKa = 4.78GNYY438 pKa = 10.1QITDD442 pKa = 3.09IFIYY446 pKa = 10.19EE447 pKa = 3.95

Molecular weight: 49.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W7N0U0|A0A2W7N0U0_9BACI Uncharacterized protein OS=Psychrobacillus insolitus OX=1461 GN=C7437_105172 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNTRR10 pKa = 11.84KK11 pKa = 9.67RR12 pKa = 11.84AKK14 pKa = 9.79NHH16 pKa = 4.64GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.52NGRR28 pKa = 11.84RR29 pKa = 11.84IIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.73VLSAA44 pKa = 4.05

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3267

0

3267

919246

27

1756

281.4

31.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.042

0.645 ± 0.013

5.005 ± 0.033

7.666 ± 0.056

4.591 ± 0.039

6.712 ± 0.039

1.986 ± 0.021

8.297 ± 0.039

6.809 ± 0.039

9.752 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.801 ± 0.022

4.458 ± 0.033

3.433 ± 0.025

3.699 ± 0.033

3.752 ± 0.033

6.131 ± 0.029

5.696 ± 0.026

7.236 ± 0.035

0.939 ± 0.015

3.385 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski