Bacillus virus Glittering

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PX72|U5PX72_9CAUD Uncharacterized protein OS=Bacillus virus Glittering OX=1406784 GN=Glittering_5 PE=4 SV=1
MM1 pKa = 8.18DD2 pKa = 4.03EE3 pKa = 4.36QKK5 pKa = 11.19DD6 pKa = 4.22GLWCTACQTNCCDD19 pKa = 3.26EE20 pKa = 4.14HH21 pKa = 6.69FKK23 pKa = 10.91PFSIDD28 pKa = 5.29CLACWEE34 pKa = 4.63DD35 pKa = 3.62KK36 pKa = 11.03CDD38 pKa = 3.99GNHH41 pKa = 6.1EE42 pKa = 4.25EE43 pKa = 4.5EE44 pKa = 4.37NN45 pKa = 3.7

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PTX5|U5PTX5_9CAUD Uncharacterized protein OS=Bacillus virus Glittering OX=1406784 GN=Glittering_24 PE=4 SV=1
MM1 pKa = 7.21AQVTVNFNGLDD12 pKa = 3.44ARR14 pKa = 11.84LKK16 pKa = 10.68QIAKK20 pKa = 8.69EE21 pKa = 4.15CQDD24 pKa = 2.78FRR26 pKa = 11.84LPLRR30 pKa = 11.84QSSVYY35 pKa = 10.79LEE37 pKa = 4.35GSMSRR42 pKa = 11.84RR43 pKa = 11.84FSQGGGSKK51 pKa = 10.65GKK53 pKa = 9.22WKK55 pKa = 10.46SLSPATIKK63 pKa = 10.43RR64 pKa = 11.84HH65 pKa = 4.35PHH67 pKa = 5.89RR68 pKa = 11.84SGGTPLNDD76 pKa = 3.33TGRR79 pKa = 11.84LRR81 pKa = 11.84SSVSSGAVKK90 pKa = 10.45QFTPKK95 pKa = 10.5RR96 pKa = 11.84LTYY99 pKa = 10.62SIGSNVKK106 pKa = 9.14YY107 pKa = 10.82ASVHH111 pKa = 5.19NFGSGRR117 pKa = 11.84IPRR120 pKa = 11.84RR121 pKa = 11.84EE122 pKa = 3.66FMYY125 pKa = 10.46TDD127 pKa = 3.29SKK129 pKa = 11.46DD130 pKa = 3.31EE131 pKa = 4.1RR132 pKa = 11.84EE133 pKa = 4.19INKK136 pKa = 8.83VFADD140 pKa = 4.04YY141 pKa = 10.5IRR143 pKa = 11.84RR144 pKa = 11.84IAQQ147 pKa = 3.09

Molecular weight: 16.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14801

41

1030

192.2

21.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.256 ± 0.388

0.932 ± 0.166

6.283 ± 0.301

7.925 ± 0.402

4.5 ± 0.183

6.723 ± 0.309

2.135 ± 0.183

6.831 ± 0.18

7.932 ± 0.436

7.851 ± 0.367

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.19

4.966 ± 0.334

3.189 ± 0.153

3.689 ± 0.154

4.52 ± 0.262

6.263 ± 0.331

5.858 ± 0.307

6.398 ± 0.232

1.115 ± 0.101

3.878 ± 0.234
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski