Ruegeria phage 45A6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X9HW69|A0A1X9HW69_9CAUD Uncharacterized protein OS=Ruegeria phage 45A6 OX=1851646 PE=4 SV=1
MM1 pKa = 7.51GKK3 pKa = 9.84KK4 pKa = 10.0DD5 pKa = 4.09DD6 pKa = 5.47LIAKK10 pKa = 8.17ATEE13 pKa = 4.04LGIEE17 pKa = 4.2LDD19 pKa = 3.84STEE22 pKa = 4.42TIADD26 pKa = 4.26LEE28 pKa = 4.37AKK30 pKa = 9.97IAAHH34 pKa = 6.52EE35 pKa = 4.06ATADD39 pKa = 3.75GEE41 pKa = 4.74GEE43 pKa = 4.17SDD45 pKa = 4.16VQPEE49 pKa = 4.01AADD52 pKa = 3.57AGATSEE58 pKa = 4.38PDD60 pKa = 3.12EE61 pKa = 4.15QAEE64 pKa = 4.41DD65 pKa = 3.48EE66 pKa = 4.47GDD68 pKa = 3.97AEE70 pKa = 5.29GGTADD75 pKa = 3.77TATVPVGSSAWALAALASGEE95 pKa = 4.03RR96 pKa = 11.84VMRR99 pKa = 11.84EE100 pKa = 3.04IWTDD104 pKa = 3.2NHH106 pKa = 6.66HH107 pKa = 7.31LDD109 pKa = 4.1PAHH112 pKa = 6.36IRR114 pKa = 11.84AGIRR118 pKa = 11.84LTFEE122 pKa = 4.9DD123 pKa = 4.73VFANDD128 pKa = 3.35WKK130 pKa = 11.27VVV132 pKa = 3.26

Molecular weight: 13.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X9HXA5|A0A1X9HXA5_9CAUD Uncharacterized protein OS=Ruegeria phage 45A6 OX=1851646 PE=4 SV=1
MM1 pKa = 7.28TRR3 pKa = 11.84PLANITRR10 pKa = 11.84EE11 pKa = 3.99ALEE14 pKa = 4.25PLWLRR19 pKa = 11.84HH20 pKa = 6.53DD21 pKa = 3.78IPTEE25 pKa = 4.53RR26 pKa = 11.84IASALGVTRR35 pKa = 11.84QGLSYY40 pKa = 10.42KK41 pKa = 10.28ARR43 pKa = 11.84SLGLPSRR50 pKa = 11.84EE51 pKa = 4.1KK52 pKa = 10.16NRR54 pKa = 11.84KK55 pKa = 9.14RR56 pKa = 11.84LVDD59 pKa = 4.36DD60 pKa = 3.91DD61 pKa = 4.22TFTRR65 pKa = 11.84MWMAGVSSTEE75 pKa = 3.89MAEE78 pKa = 4.2HH79 pKa = 6.58FWYY82 pKa = 10.13SCRR85 pKa = 11.84SAITARR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84LLKK96 pKa = 10.74LPARR100 pKa = 11.84TRR102 pKa = 11.84GRR104 pKa = 11.84GGKK107 pKa = 8.76NAGGWLEE114 pKa = 4.59TITLAQFAEE123 pKa = 4.29MEE125 pKa = 4.51LARR128 pKa = 11.84KK129 pKa = 8.44MKK131 pKa = 10.43EE132 pKa = 3.43RR133 pKa = 11.84DD134 pKa = 3.13

Molecular weight: 15.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

17102

41

3912

285.0

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.923 ± 0.576

0.719 ± 0.181

7.064 ± 0.326

6.859 ± 0.36

3.216 ± 0.12

8.824 ± 0.3

1.959 ± 0.273

4.438 ± 0.2

4.257 ± 0.324

7.52 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.631 ± 0.163

2.929 ± 0.222

5.42 ± 0.213

3.947 ± 0.251

7.613 ± 0.309

5.339 ± 0.412

5.596 ± 0.253

5.935 ± 0.266

1.643 ± 0.2

2.169 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski