Amino acid dipepetide frequency for Nocardioides sp. S-1144

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.721AlaAla: 20.721 ± 0.211
0.987AlaCys: 0.987 ± 0.032
8.858AlaAsp: 8.858 ± 0.082
7.93AlaGlu: 7.93 ± 0.089
3.43AlaPhe: 3.43 ± 0.063
13.597AlaGly: 13.597 ± 0.116
2.793AlaHis: 2.793 ± 0.057
3.927AlaIle: 3.927 ± 0.072
2.043AlaLys: 2.043 ± 0.05
13.805AlaLeu: 13.805 ± 0.155
2.56AlaMet: 2.56 ± 0.043
1.858AlaAsn: 1.858 ± 0.04
6.956AlaPro: 6.956 ± 0.082
3.177AlaGln: 3.177 ± 0.053
10.21AlaArg: 10.21 ± 0.093
6.169AlaSer: 6.169 ± 0.08
8.146AlaThr: 8.146 ± 0.084
12.891AlaVal: 12.891 ± 0.142
2.029AlaTrp: 2.029 ± 0.046
2.241AlaTyr: 2.241 ± 0.043
0.0AlaXaa: 0.0 ± 0.0
Cys
0.867CysAla: 0.867 ± 0.025
0.084CysCys: 0.084 ± 0.008
0.483CysAsp: 0.483 ± 0.019
0.358CysGlu: 0.358 ± 0.015
0.19CysPhe: 0.19 ± 0.011
0.843CysGly: 0.843 ± 0.027
0.182CysHis: 0.182 ± 0.012
0.153CysIle: 0.153 ± 0.01
0.067CysLys: 0.067 ± 0.008
0.684CysLeu: 0.684 ± 0.022
0.098CysMet: 0.098 ± 0.009
0.131CysAsn: 0.131 ± 0.011
0.435CysPro: 0.435 ± 0.017
0.179CysGln: 0.179 ± 0.01
0.563CysArg: 0.563 ± 0.022
0.414CysSer: 0.414 ± 0.018
0.457CysThr: 0.457 ± 0.021
0.635CysVal: 0.635 ± 0.023
0.102CysTrp: 0.102 ± 0.01
0.152CysTyr: 0.152 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.785AspAla: 8.785 ± 0.085
0.334AspCys: 0.334 ± 0.017
5.629AspAsp: 5.629 ± 0.084
4.347AspGlu: 4.347 ± 0.067
1.571AspPhe: 1.571 ± 0.041
7.008AspGly: 7.008 ± 0.1
1.589AspHis: 1.589 ± 0.038
1.541AspIle: 1.541 ± 0.036
0.94AspLys: 0.94 ± 0.032
7.953AspLeu: 7.953 ± 0.092
0.72AspMet: 0.72 ± 0.02
0.927AspAsn: 0.927 ± 0.028
4.979AspPro: 4.979 ± 0.065
1.778AspGln: 1.778 ± 0.038
5.283AspArg: 5.283 ± 0.064
2.301AspSer: 2.301 ± 0.045
2.997AspThr: 2.997 ± 0.049
7.268AspVal: 7.268 ± 0.083
0.929AspTrp: 0.929 ± 0.029
1.112AspTyr: 1.112 ± 0.03
0.0AspXaa: 0.0 ± 0.0
Glu
7.122GluAla: 7.122 ± 0.082
0.322GluCys: 0.322 ± 0.016
3.008GluAsp: 3.008 ± 0.054
3.234GluGlu: 3.234 ± 0.059
1.34GluPhe: 1.34 ± 0.033
4.188GluGly: 4.188 ± 0.054
1.703GluHis: 1.703 ± 0.041
2.113GluIle: 2.113 ± 0.046
1.122GluLys: 1.122 ± 0.038
6.077GluLeu: 6.077 ± 0.063
0.915GluMet: 0.915 ± 0.028
0.856GluAsn: 0.856 ± 0.028
3.457GluPro: 3.457 ± 0.055
2.201GluGln: 2.201 ± 0.042
5.235GluArg: 5.235 ± 0.068
2.519GluSer: 2.519 ± 0.042
2.899GluThr: 2.899 ± 0.054
5.76GluVal: 5.76 ± 0.075
0.722GluTrp: 0.722 ± 0.025
0.873GluTyr: 0.873 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
3.425PheAla: 3.425 ± 0.055
0.239PheCys: 0.239 ± 0.013
2.057PheAsp: 2.057 ± 0.042
1.532PheGlu: 1.532 ± 0.038
0.787PhePhe: 0.787 ± 0.027
2.896PheGly: 2.896 ± 0.057
0.561PheHis: 0.561 ± 0.022
0.649PheIle: 0.649 ± 0.023
0.404PheLys: 0.404 ± 0.018
2.566PheLeu: 2.566 ± 0.055
0.356PheMet: 0.356 ± 0.016
0.513PheAsn: 0.513 ± 0.019
1.215PhePro: 1.215 ± 0.032
0.621PheGln: 0.621 ± 0.021
1.718PheArg: 1.718 ± 0.037
1.329PheSer: 1.329 ± 0.026
1.784PheThr: 1.784 ± 0.038
2.649PheVal: 2.649 ± 0.048
0.41PheTrp: 0.41 ± 0.018
0.562PheTyr: 0.562 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
11.422GlyAla: 11.422 ± 0.112
0.808GlyCys: 0.808 ± 0.025
5.952GlyAsp: 5.952 ± 0.08
5.039GlyGlu: 5.039 ± 0.065
2.868GlyPhe: 2.868 ± 0.047
8.91GlyGly: 8.91 ± 0.107
2.128GlyHis: 2.128 ± 0.044
3.278GlyIle: 3.278 ± 0.05
1.762GlyLys: 1.762 ± 0.042
9.726GlyLeu: 9.726 ± 0.088
1.783GlyMet: 1.783 ± 0.035
1.602GlyAsn: 1.602 ± 0.043
5.115GlyPro: 5.115 ± 0.068
2.408GlyGln: 2.408 ± 0.051
7.989GlyArg: 7.989 ± 0.081
5.541GlySer: 5.541 ± 0.068
6.293GlyThr: 6.293 ± 0.077
8.675GlyVal: 8.675 ± 0.09
1.724GlyTrp: 1.724 ± 0.036
2.197GlyTyr: 2.197 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.668HisAla: 2.668 ± 0.052
0.178HisCys: 0.178 ± 0.011
1.8HisAsp: 1.8 ± 0.041
1.335HisGlu: 1.335 ± 0.034
0.491HisPhe: 0.491 ± 0.02
2.311HisGly: 2.311 ± 0.044
0.728HisHis: 0.728 ± 0.023
0.365HisIle: 0.365 ± 0.017
0.229HisLys: 0.229 ± 0.012
2.528HisLeu: 2.528 ± 0.044
0.274HisMet: 0.274 ± 0.013
0.294HisAsn: 0.294 ± 0.015
1.618HisPro: 1.618 ± 0.033
0.642HisGln: 0.642 ± 0.024
1.997HisArg: 1.997 ± 0.041
0.735HisSer: 0.735 ± 0.025
1.109HisThr: 1.109 ± 0.032
2.245HisVal: 2.245 ± 0.045
0.299HisTrp: 0.299 ± 0.014
0.425HisTyr: 0.425 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
4.226IleAla: 4.226 ± 0.081
0.246IleCys: 0.246 ± 0.014
2.252IleAsp: 2.252 ± 0.046
1.938IleGlu: 1.938 ± 0.043
0.712IlePhe: 0.712 ± 0.026
3.305IleGly: 3.305 ± 0.054
0.512IleHis: 0.512 ± 0.021
0.905IleIle: 0.905 ± 0.031
0.624IleLys: 0.624 ± 0.022
2.314IleLeu: 2.314 ± 0.046
0.376IleMet: 0.376 ± 0.021
0.659IleAsn: 0.659 ± 0.027
1.475IlePro: 1.475 ± 0.037
0.675IleGln: 0.675 ± 0.022
1.871IleArg: 1.871 ± 0.041
1.577IleSer: 1.577 ± 0.038
2.079IleThr: 2.079 ± 0.041
2.681IleVal: 2.681 ± 0.045
0.32IleTrp: 0.32 ± 0.014
0.524IleTyr: 0.524 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
2.054LysAla: 2.054 ± 0.054
0.065LysCys: 0.065 ± 0.009
0.949LysAsp: 0.949 ± 0.029
0.836LysGlu: 0.836 ± 0.034
0.396LysPhe: 0.396 ± 0.016
1.272LysGly: 1.272 ± 0.039
0.386LysHis: 0.386 ± 0.017
0.733LysIle: 0.733 ± 0.026
0.587LysLys: 0.587 ± 0.03
1.409LysLeu: 1.409 ± 0.038
0.322LysMet: 0.322 ± 0.017
0.384LysAsn: 0.384 ± 0.017
0.981LysPro: 0.981 ± 0.03
0.6LysGln: 0.6 ± 0.021
1.131LysArg: 1.131 ± 0.035
0.879LysSer: 0.879 ± 0.031
0.98LysThr: 0.98 ± 0.032
1.681LysVal: 1.681 ± 0.046
0.165LysTrp: 0.165 ± 0.012
0.325LysTyr: 0.325 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
15.543LeuAla: 15.543 ± 0.13
0.695LeuCys: 0.695 ± 0.024
8.026LeuAsp: 8.026 ± 0.089
5.396LeuGlu: 5.396 ± 0.066
2.291LeuPhe: 2.291 ± 0.047
10.196LeuGly: 10.196 ± 0.094
2.107LeuHis: 2.107 ± 0.049
2.239LeuIle: 2.239 ± 0.054
1.467LeuLys: 1.467 ± 0.037
10.939LeuLeu: 10.939 ± 0.14
1.441LeuMet: 1.441 ± 0.034
1.453LeuAsn: 1.453 ± 0.04
5.742LeuPro: 5.742 ± 0.058
2.274LeuGln: 2.274 ± 0.041
8.116LeuArg: 8.116 ± 0.087
4.867LeuSer: 4.867 ± 0.059
6.489LeuThr: 6.489 ± 0.084
11.478LeuVal: 11.478 ± 0.139
1.128LeuTrp: 1.128 ± 0.032
1.479LeuTyr: 1.479 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
2.059MetAla: 2.059 ± 0.042
0.121MetCys: 0.121 ± 0.01
0.793MetAsp: 0.793 ± 0.023
0.652MetGlu: 0.652 ± 0.02
0.43MetPhe: 0.43 ± 0.022
1.245MetGly: 1.245 ± 0.033
0.3MetHis: 0.3 ± 0.017
0.586MetIle: 0.586 ± 0.023
0.375MetLys: 0.375 ± 0.018
1.679MetLeu: 1.679 ± 0.036
0.295MetMet: 0.295 ± 0.014
0.353MetAsn: 0.353 ± 0.017
1.083MetPro: 1.083 ± 0.026
0.39MetGln: 0.39 ± 0.017
1.357MetArg: 1.357 ± 0.035
1.359MetSer: 1.359 ± 0.031
1.643MetThr: 1.643 ± 0.033
1.417MetVal: 1.417 ± 0.033
0.177MetTrp: 0.177 ± 0.012
0.234MetTyr: 0.234 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.001AsnAla: 2.001 ± 0.047
0.118AsnCys: 0.118 ± 0.01
0.985AsnAsp: 0.985 ± 0.027
0.707AsnGlu: 0.707 ± 0.024
0.43AsnPhe: 0.43 ± 0.018
1.544AsnGly: 1.544 ± 0.04
0.342AsnHis: 0.342 ± 0.015
0.573AsnIle: 0.573 ± 0.025
0.315AsnLys: 0.315 ± 0.016
1.735AsnLeu: 1.735 ± 0.038
0.24AsnMet: 0.24 ± 0.014
0.391AsnAsn: 0.391 ± 0.022
1.317AsnPro: 1.317 ± 0.043
0.46AsnGln: 0.46 ± 0.019
1.163AsnArg: 1.163 ± 0.031
0.679AsnSer: 0.679 ± 0.023
0.93AsnThr: 0.93 ± 0.031
1.42AsnVal: 1.42 ± 0.038
0.201AsnTrp: 0.201 ± 0.013
0.343AsnTyr: 0.343 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
8.258ProAla: 8.258 ± 0.094
0.297ProCys: 0.297 ± 0.015
4.959ProAsp: 4.959 ± 0.067
3.642ProGlu: 3.642 ± 0.056
1.466ProPhe: 1.466 ± 0.031
6.374ProGly: 6.374 ± 0.075
1.248ProHis: 1.248 ± 0.035
1.436ProIle: 1.436 ± 0.036
0.743ProLys: 0.743 ± 0.032
4.918ProLeu: 4.918 ± 0.061
0.981ProMet: 0.981 ± 0.026
0.739ProAsn: 0.739 ± 0.027
3.366ProPro: 3.366 ± 0.067
1.338ProGln: 1.338 ± 0.038
4.073ProArg: 4.073 ± 0.063
3.224ProSer: 3.224 ± 0.056
3.924ProThr: 3.924 ± 0.072
5.607ProVal: 5.607 ± 0.067
0.927ProTrp: 0.927 ± 0.028
1.042ProTyr: 1.042 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
3.285GlnAla: 3.285 ± 0.054
0.15GlnCys: 0.15 ± 0.011
1.326GlnAsp: 1.326 ± 0.034
1.247GlnGlu: 1.247 ± 0.031
0.614GlnPhe: 0.614 ± 0.025
2.055GlnGly: 2.055 ± 0.042
0.688GlnHis: 0.688 ± 0.023
0.822GlnIle: 0.822 ± 0.027
0.46GlnLys: 0.46 ± 0.019
2.741GlnLeu: 2.741 ± 0.046
0.45GlnMet: 0.45 ± 0.021
0.425GlnAsn: 0.425 ± 0.019
1.564GlnPro: 1.564 ± 0.041
1.075GlnGln: 1.075 ± 0.032
2.375GlnArg: 2.375 ± 0.045
1.128GlnSer: 1.128 ± 0.032
1.305GlnThr: 1.305 ± 0.03
2.873GlnVal: 2.873 ± 0.047
0.416GlnTrp: 0.416 ± 0.018
0.441GlnTyr: 0.441 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
9.899ArgAla: 9.899 ± 0.112
0.563ArgCys: 0.563 ± 0.022
4.803ArgAsp: 4.803 ± 0.07
4.31ArgGlu: 4.31 ± 0.063
2.25ArgPhe: 2.25 ± 0.04
6.169ArgGly: 6.169 ± 0.077
1.989ArgHis: 1.989 ± 0.04
2.75ArgIle: 2.75 ± 0.049
1.242ArgLys: 1.242 ± 0.037
8.601ArgLeu: 8.601 ± 0.088
1.668ArgMet: 1.668 ± 0.039
1.16ArgAsn: 1.16 ± 0.032
4.853ArgPro: 4.853 ± 0.075
2.041ArgGln: 2.041 ± 0.043
8.096ArgArg: 8.096 ± 0.107
4.354ArgSer: 4.354 ± 0.059
5.1ArgThr: 5.1 ± 0.06
6.953ArgVal: 6.953 ± 0.087
1.424ArgTrp: 1.424 ± 0.036
1.517ArgTyr: 1.517 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.108SerAla: 6.108 ± 0.069
0.375SerCys: 0.375 ± 0.02
2.923SerAsp: 2.923 ± 0.055
2.386SerGlu: 2.386 ± 0.046
1.665SerPhe: 1.665 ± 0.043
5.507SerGly: 5.507 ± 0.064
1.018SerHis: 1.018 ± 0.028
1.678SerIle: 1.678 ± 0.041
0.81SerLys: 0.81 ± 0.028
4.863SerLeu: 4.863 ± 0.065
1.146SerMet: 1.146 ± 0.027
0.866SerAsn: 0.866 ± 0.03
2.976SerPro: 2.976 ± 0.05
1.151SerGln: 1.151 ± 0.032
3.735SerArg: 3.735 ± 0.062
3.091SerSer: 3.091 ± 0.052
3.429SerThr: 3.429 ± 0.054
4.389SerVal: 4.389 ± 0.065
0.905SerTrp: 0.905 ± 0.027
1.257SerTyr: 1.257 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
7.817ThrAla: 7.817 ± 0.098
0.481ThrCys: 0.481 ± 0.02
3.955ThrAsp: 3.955 ± 0.057
2.992ThrGlu: 2.992 ± 0.05
1.877ThrPhe: 1.877 ± 0.036
6.409ThrGly: 6.409 ± 0.08
1.298ThrHis: 1.298 ± 0.029
2.126ThrIle: 2.126 ± 0.04
0.952ThrLys: 0.952 ± 0.029
5.942ThrLeu: 5.942 ± 0.074
1.069ThrMet: 1.069 ± 0.03
1.036ThrAsn: 1.036 ± 0.029
4.113ThrPro: 4.113 ± 0.06
1.359ThrGln: 1.359 ± 0.037
4.26ThrArg: 4.26 ± 0.056
3.685ThrSer: 3.685 ± 0.06
4.687ThrThr: 4.687 ± 0.069
5.967ThrVal: 5.967 ± 0.083
1.078ThrTrp: 1.078 ± 0.03
1.444ThrTyr: 1.444 ± 0.038
0.0ThrXaa: 0.0 ± 0.0
Val
14.02ValAla: 14.02 ± 0.144
0.708ValCys: 0.708 ± 0.025
6.871ValAsp: 6.871 ± 0.077
6.165ValGlu: 6.165 ± 0.073
2.364ValPhe: 2.364 ± 0.046
8.585ValGly: 8.585 ± 0.105
2.042ValHis: 2.042 ± 0.043
2.572ValIle: 2.572 ± 0.052
1.453ValLys: 1.453 ± 0.044
11.161ValLeu: 11.161 ± 0.113
1.4ValMet: 1.4 ± 0.035
1.603ValAsn: 1.603 ± 0.037
5.586ValPro: 5.586 ± 0.071
2.084ValGln: 2.084 ± 0.039
7.733ValArg: 7.733 ± 0.086
4.559ValSer: 4.559 ± 0.057
6.292ValThr: 6.292 ± 0.09
12.618ValVal: 12.618 ± 0.153
1.132ValTrp: 1.132 ± 0.03
1.417ValTyr: 1.417 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.618TrpAla: 1.618 ± 0.037
0.15TrpCys: 0.15 ± 0.01
0.873TrpAsp: 0.873 ± 0.025
0.645TrpGlu: 0.645 ± 0.023
0.525TrpPhe: 0.525 ± 0.022
1.062TrpGly: 1.062 ± 0.025
0.334TrpHis: 0.334 ± 0.017
0.472TrpIle: 0.472 ± 0.018
0.256TrpLys: 0.256 ± 0.014
1.667TrpLeu: 1.667 ± 0.037
0.271TrpMet: 0.271 ± 0.013
0.331TrpAsn: 0.331 ± 0.015
0.758TrpPro: 0.758 ± 0.026
0.481TrpGln: 0.481 ± 0.02
1.288TrpArg: 1.288 ± 0.03
0.984TrpSer: 0.984 ± 0.027
0.996TrpThr: 0.996 ± 0.03
1.336TrpVal: 1.336 ± 0.033
0.376TrpTrp: 0.376 ± 0.019
0.313TrpTyr: 0.313 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.431TyrAla: 2.431 ± 0.038
0.158TyrCys: 0.158 ± 0.009
1.489TyrAsp: 1.489 ± 0.037
0.977TyrGlu: 0.977 ± 0.028
0.577TyrPhe: 0.577 ± 0.022
1.743TyrGly: 1.743 ± 0.041
0.322TyrHis: 0.322 ± 0.017
0.354TyrIle: 0.354 ± 0.016
0.281TyrLys: 0.281 ± 0.016
2.059TyrLeu: 2.059 ± 0.037
0.186TyrMet: 0.186 ± 0.01
0.31TyrAsn: 0.31 ± 0.017
0.972TyrPro: 0.972 ± 0.031
0.512TyrGln: 0.512 ± 0.02
1.546TyrArg: 1.546 ± 0.04
0.883TyrSer: 0.883 ± 0.028
1.006TyrThr: 1.006 ± 0.031
1.822TyrVal: 1.822 ± 0.04
0.271TyrTrp: 0.271 ± 0.016
0.391TyrTyr: 0.391 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4010 proteins (1318656 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski