Amino acid dipepetide frequency for Pelomonas sp. Root1444

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.931AlaAla: 20.931 ± 0.176	1.248AlaCys: 1.248 ± 0.03	7.104AlaAsp: 7.104 ± 0.074	7.541AlaGlu: 7.541 ± 0.075	4.091AlaPhe: 4.091 ± 0.051	12.143AlaGly: 12.143 ± 0.11	2.698AlaHis: 2.698 ± 0.04	4.589AlaIle: 4.589 ± 0.056	3.916AlaLys: 3.916 ± 0.064	16.732AlaLeu: 16.732 ± 0.158	3.345AlaMet: 3.345 ± 0.049	2.936AlaAsn: 2.936 ± 0.058	6.888AlaPro: 6.888 ± 0.087	6.063AlaGln: 6.063 ± 0.079	9.749AlaArg: 9.749 ± 0.094	6.915AlaSer: 6.915 ± 0.078	6.608AlaThr: 6.608 ± 0.08	9.524AlaVal: 9.524 ± 0.085	2.387AlaTrp: 2.387 ± 0.049	2.516AlaTyr: 2.516 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	1.085CysAla: 1.085 ± 0.028	0.103CysCys: 0.103 ± 0.008	0.51CysAsp: 0.51 ± 0.018	0.46CysGlu: 0.46 ± 0.019	0.288CysPhe: 0.288 ± 0.015	0.9CysGly: 0.9 ± 0.027	0.229CysHis: 0.229 ± 0.012	0.339CysIle: 0.339 ± 0.016	0.208CysLys: 0.208 ± 0.01	0.745CysLeu: 0.745 ± 0.025	0.171CysMet: 0.171 ± 0.01	0.215CysAsn: 0.215 ± 0.011	0.446CysPro: 0.446 ± 0.015	0.222CysGln: 0.222 ± 0.009	0.55CysArg: 0.55 ± 0.017	0.466CysSer: 0.466 ± 0.016	0.476CysThr: 0.476 ± 0.017	0.619CysVal: 0.619 ± 0.019	0.129CysTrp: 0.129 ± 0.01	0.165CysTyr: 0.165 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.95AspAla: 7.95 ± 0.072	0.441AspCys: 0.441 ± 0.018	2.916AspAsp: 2.916 ± 0.047	3.133AspGlu: 3.133 ± 0.047	2.027AspPhe: 2.027 ± 0.038	5.387AspGly: 5.387 ± 0.08	1.085AspHis: 1.085 ± 0.03	2.256AspIle: 2.256 ± 0.035	1.674AspLys: 1.674 ± 0.037	5.361AspLeu: 5.361 ± 0.07	1.034AspMet: 1.034 ± 0.023	1.231AspAsn: 1.231 ± 0.039	2.979AspPro: 2.979 ± 0.045	1.528AspGln: 1.528 ± 0.032	3.428AspArg: 3.428 ± 0.043	2.214AspSer: 2.214 ± 0.043	2.512AspThr: 2.512 ± 0.068	4.006AspVal: 4.006 ± 0.049	1.139AspTrp: 1.139 ± 0.028	1.315AspTyr: 1.315 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	7.355GluAla: 7.355 ± 0.085	0.313GluCys: 0.313 ± 0.015	2.096GluAsp: 2.096 ± 0.042	2.069GluGlu: 2.069 ± 0.04	1.699GluPhe: 1.699 ± 0.032	3.818GluGly: 3.818 ± 0.047	1.239GluHis: 1.239 ± 0.028	2.319GluIle: 2.319 ± 0.045	1.49GluLys: 1.49 ± 0.032	6.359GluLeu: 6.359 ± 0.072	1.102GluMet: 1.102 ± 0.027	1.07GluAsn: 1.07 ± 0.033	2.824GluPro: 2.824 ± 0.048	2.517GluGln: 2.517 ± 0.039	4.871GluArg: 4.871 ± 0.056	2.174GluSer: 2.174 ± 0.036	2.317GluThr: 2.317 ± 0.041	3.848GluVal: 3.848 ± 0.059	0.702GluTrp: 0.702 ± 0.021	0.861GluTyr: 0.861 ± 0.027	0.0GluXaa: 0.0 ± 0.0
Phe	4.222PheAla: 4.222 ± 0.059	0.327PheCys: 0.327 ± 0.014	2.587PheAsp: 2.587 ± 0.037	2.06PheGlu: 2.06 ± 0.034	1.244PhePhe: 1.244 ± 0.033	3.392PheGly: 3.392 ± 0.047	0.733PheHis: 0.733 ± 0.02	1.431PheIle: 1.431 ± 0.033	1.213PheLys: 1.213 ± 0.03	2.64PheLeu: 2.64 ± 0.044	0.728PheMet: 0.728 ± 0.021	1.115PheAsn: 1.115 ± 0.027	1.353PhePro: 1.353 ± 0.03	0.989PheGln: 0.989 ± 0.025	1.743PheArg: 1.743 ± 0.037	2.019PheSer: 2.019 ± 0.03	1.926PheThr: 1.926 ± 0.036	2.579PheVal: 2.579 ± 0.044	0.52PheTrp: 0.52 ± 0.017	0.804PheTyr: 0.804 ± 0.024	0.0PheXaa: 0.0 ± 0.0
Gly	9.945GlyAla: 9.945 ± 0.102	0.89GlyCys: 0.89 ± 0.024	4.311GlyAsp: 4.311 ± 0.075	4.642GlyGlu: 4.642 ± 0.05	3.393GlyPhe: 3.393 ± 0.049	7.627GlyGly: 7.627 ± 0.117	2.039GlyHis: 2.039 ± 0.039	3.561GlyIle: 3.561 ± 0.051	2.979GlyLys: 2.979 ± 0.055	9.899GlyLeu: 9.899 ± 0.084	2.098GlyMet: 2.098 ± 0.041	2.216GlyAsn: 2.216 ± 0.081	3.303GlyPro: 3.303 ± 0.053	3.678GlyGln: 3.678 ± 0.044	6.346GlyArg: 6.346 ± 0.067	4.766GlySer: 4.766 ± 0.069	4.429GlyThr: 4.429 ± 0.075	6.571GlyVal: 6.571 ± 0.07	1.751GlyTrp: 1.751 ± 0.034	2.136GlyTyr: 2.136 ± 0.033	0.002GlyXaa: 0.002 ± 0.001
His	2.966HisAla: 2.966 ± 0.052	0.269HisCys: 0.269 ± 0.013	1.206HisAsp: 1.206 ± 0.026	1.192HisGlu: 1.192 ± 0.024	0.868HisPhe: 0.868 ± 0.024	2.152HisGly: 2.152 ± 0.038	0.64HisHis: 0.64 ± 0.021	0.777HisIle: 0.777 ± 0.023	0.51HisLys: 0.51 ± 0.017	2.24HisLeu: 2.24 ± 0.04	0.402HisMet: 0.402 ± 0.016	0.485HisAsn: 0.485 ± 0.019	1.385HisPro: 1.385 ± 0.029	0.756HisGln: 0.756 ± 0.019	1.531HisArg: 1.531 ± 0.031	0.902HisSer: 0.902 ± 0.022	0.902HisThr: 0.902 ± 0.023	1.575HisVal: 1.575 ± 0.032	0.473HisTrp: 0.473 ± 0.017	0.578HisTyr: 0.578 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.757IleAla: 5.757 ± 0.079	0.346IleCys: 0.346 ± 0.014	2.758IleAsp: 2.758 ± 0.041	2.726IleGlu: 2.726 ± 0.043	1.017IlePhe: 1.017 ± 0.025	3.804IleGly: 3.804 ± 0.058	0.75IleHis: 0.75 ± 0.02	1.038IleIle: 1.038 ± 0.028	1.338IleLys: 1.338 ± 0.03	2.617IleLeu: 2.617 ± 0.048	0.531IleMet: 0.531 ± 0.02	1.222IleAsn: 1.222 ± 0.031	1.713IlePro: 1.713 ± 0.038	1.206IleGln: 1.206 ± 0.027	2.221IleArg: 2.221 ± 0.04	2.042IleSer: 2.042 ± 0.032	2.238IleThr: 2.238 ± 0.041	3.081IleVal: 3.081 ± 0.046	0.437IleTrp: 0.437 ± 0.016	0.812IleTyr: 0.812 ± 0.019	0.0IleXaa: 0.0 ± 0.0
Lys	4.201LysAla: 4.201 ± 0.062	0.129LysCys: 0.129 ± 0.01	1.52LysAsp: 1.52 ± 0.035	1.254LysGlu: 1.254 ± 0.032	0.883LysPhe: 0.883 ± 0.025	2.269LysGly: 2.269 ± 0.041	0.623LysHis: 0.623 ± 0.019	1.098LysIle: 1.098 ± 0.031	1.14LysLys: 1.14 ± 0.036	3.601LysLeu: 3.601 ± 0.062	0.631LysMet: 0.631 ± 0.021	0.786LysAsn: 0.786 ± 0.023	2.303LysPro: 2.303 ± 0.042	1.069LysGln: 1.069 ± 0.028	2.253LysArg: 2.253 ± 0.036	1.509LysSer: 1.509 ± 0.034	1.75LysThr: 1.75 ± 0.037	2.258LysVal: 2.258 ± 0.044	0.309LysTrp: 0.309 ± 0.014	0.546LysTyr: 0.546 ± 0.021	0.0LysXaa: 0.0 ± 0.0
Leu	17.081LeuAla: 17.081 ± 0.131	0.916LeuCys: 0.916 ± 0.025	6.255LeuAsp: 6.255 ± 0.066	4.635LeuGlu: 4.635 ± 0.065	3.269LeuPhe: 3.269 ± 0.052	8.986LeuGly: 8.986 ± 0.082	2.351LeuHis: 2.351 ± 0.048	4.05LeuIle: 4.05 ± 0.045	3.751LeuLys: 3.751 ± 0.052	12.254LeuLeu: 12.254 ± 0.143	2.494LeuMet: 2.494 ± 0.036	2.816LeuAsn: 2.816 ± 0.042	6.789LeuPro: 6.789 ± 0.079	4.542LeuGln: 4.542 ± 0.05	8.554LeuArg: 8.554 ± 0.089	6.091LeuSer: 6.091 ± 0.068	5.785LeuThr: 5.785 ± 0.07	7.748LeuVal: 7.748 ± 0.077	1.541LeuTrp: 1.541 ± 0.035	1.969LeuTyr: 1.969 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.918MetAla: 2.918 ± 0.045	0.135MetCys: 0.135 ± 0.009	1.032MetAsp: 1.032 ± 0.026	0.813MetGlu: 0.813 ± 0.022	0.616MetPhe: 0.616 ± 0.019	1.648MetGly: 1.648 ± 0.032	0.477MetHis: 0.477 ± 0.017	0.73MetIle: 0.73 ± 0.019	0.951MetLys: 0.951 ± 0.024	2.505MetLeu: 2.505 ± 0.044	0.429MetMet: 0.429 ± 0.018	0.79MetAsn: 0.79 ± 0.021	1.46MetPro: 1.46 ± 0.029	0.974MetGln: 0.974 ± 0.023	1.658MetArg: 1.658 ± 0.031	1.45MetSer: 1.45 ± 0.028	1.44MetThr: 1.44 ± 0.03	1.448MetVal: 1.448 ± 0.032	0.196MetTrp: 0.196 ± 0.009	0.307MetTyr: 0.307 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	3.278AsnAla: 3.278 ± 0.054	0.235AsnCys: 0.235 ± 0.012	1.335AsnAsp: 1.335 ± 0.043	1.138AsnGlu: 1.138 ± 0.028	0.919AsnPhe: 0.919 ± 0.024	2.347AsnGly: 2.347 ± 0.056	0.509AsnHis: 0.509 ± 0.017	1.02AsnIle: 1.02 ± 0.03	0.723AsnLys: 0.723 ± 0.025	2.658AsnLeu: 2.658 ± 0.054	0.461AsnMet: 0.461 ± 0.017	0.76AsnAsn: 0.76 ± 0.03	1.717AsnPro: 1.717 ± 0.032	0.88AsnGln: 0.88 ± 0.028	1.591AsnArg: 1.591 ± 0.035	1.17AsnSer: 1.17 ± 0.029	1.352AsnThr: 1.352 ± 0.035	1.842AsnVal: 1.842 ± 0.046	0.432AsnTrp: 0.432 ± 0.016	0.683AsnTyr: 0.683 ± 0.023	0.0AsnXaa: 0.0 ± 0.0
Pro	8.133ProAla: 8.133 ± 0.094	0.346ProCys: 0.346 ± 0.014	3.333ProAsp: 3.333 ± 0.045	3.343ProGlu: 3.343 ± 0.052	1.733ProPhe: 1.733 ± 0.029	5.145ProGly: 5.145 ± 0.065	1.08ProHis: 1.08 ± 0.029	1.629ProIle: 1.629 ± 0.036	1.467ProLys: 1.467 ± 0.032	5.762ProLeu: 5.762 ± 0.063	1.24ProMet: 1.24 ± 0.027	1.265ProAsn: 1.265 ± 0.031	3.105ProPro: 3.105 ± 0.058	2.188ProGln: 2.188 ± 0.04	3.328ProArg: 3.328 ± 0.055	2.625ProSer: 2.625 ± 0.043	2.692ProThr: 2.692 ± 0.041	4.186ProVal: 4.186 ± 0.05	0.857ProTrp: 0.857 ± 0.025	1.03ProTyr: 1.03 ± 0.024	0.0ProXaa: 0.0 ± 0.0
Gln	6.305GlnAla: 6.305 ± 0.067	0.252GlnCys: 0.252 ± 0.013	1.658GlnAsp: 1.658 ± 0.031	1.334GlnGlu: 1.334 ± 0.03	1.248GlnPhe: 1.248 ± 0.026	3.187GlnGly: 3.187 ± 0.046	0.916GlnHis: 0.916 ± 0.024	1.566GlnIle: 1.566 ± 0.04	0.882GlnLys: 0.882 ± 0.024	4.704GlnLeu: 4.704 ± 0.054	0.891GlnMet: 0.891 ± 0.023	0.723GlnAsn: 0.723 ± 0.024	2.602GlnPro: 2.602 ± 0.041	1.93GlnGln: 1.93 ± 0.038	3.851GlnArg: 3.851 ± 0.066	1.73GlnSer: 1.73 ± 0.031	1.833GlnThr: 1.833 ± 0.034	3.087GlnVal: 3.087 ± 0.055	0.645GlnTrp: 0.645 ± 0.021	0.676GlnTyr: 0.676 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	8.546ArgAla: 8.546 ± 0.081	0.596ArgCys: 0.596 ± 0.021	3.847ArgAsp: 3.847 ± 0.05	4.461ArgGlu: 4.461 ± 0.064	2.903ArgPhe: 2.903 ± 0.044	5.124ArgGly: 5.124 ± 0.06	1.977ArgHis: 1.977 ± 0.036	3.293ArgIle: 3.293 ± 0.041	1.786ArgLys: 1.786 ± 0.031	8.902ArgLeu: 8.902 ± 0.094	1.797ArgMet: 1.797 ± 0.033	1.702ArgAsn: 1.702 ± 0.03	3.523ArgPro: 3.523 ± 0.047	3.053ArgGln: 3.053 ± 0.047	5.705ArgArg: 5.705 ± 0.068	3.531ArgSer: 3.531 ± 0.043	3.027ArgThr: 3.027 ± 0.039	5.444ArgVal: 5.444 ± 0.065	1.479ArgTrp: 1.479 ± 0.036	1.792ArgTyr: 1.792 ± 0.034	0.001ArgXaa: 0.001 ± 0.001
Ser	6.455SerAla: 6.455 ± 0.074	0.409SerCys: 0.409 ± 0.014	2.445SerAsp: 2.445 ± 0.044	2.233SerGlu: 2.233 ± 0.034	2.023SerPhe: 2.023 ± 0.039	5.172SerGly: 5.172 ± 0.077	1.093SerHis: 1.093 ± 0.023	1.977SerIle: 1.977 ± 0.036	1.384SerLys: 1.384 ± 0.033	5.655SerLeu: 5.655 ± 0.061	1.127SerMet: 1.127 ± 0.025	1.318SerAsn: 1.318 ± 0.038	2.796SerPro: 2.796 ± 0.045	1.818SerGln: 1.818 ± 0.029	3.444SerArg: 3.444 ± 0.046	2.773SerSer: 2.773 ± 0.055	2.857SerThr: 2.857 ± 0.059	3.676SerVal: 3.676 ± 0.044	0.87SerTrp: 0.87 ± 0.025	1.254SerTyr: 1.254 ± 0.031	0.001SerXaa: 0.001 ± 0.001
Thr	6.634ThrAla: 6.634 ± 0.074	0.336ThrCys: 0.336 ± 0.015	2.497ThrAsp: 2.497 ± 0.04	2.314ThrGlu: 2.314 ± 0.041	1.449ThrPhe: 1.449 ± 0.029	4.76ThrGly: 4.76 ± 0.088	1.055ThrHis: 1.055 ± 0.025	1.661ThrIle: 1.661 ± 0.03	1.177ThrLys: 1.177 ± 0.029	6.662ThrLeu: 6.662 ± 0.079	0.954ThrMet: 0.954 ± 0.025	1.236ThrAsn: 1.236 ± 0.036	3.614ThrPro: 3.614 ± 0.048	1.945ThrGln: 1.945 ± 0.034	3.393ThrArg: 3.393 ± 0.047	2.518ThrSer: 2.518 ± 0.049	2.892ThrThr: 2.892 ± 0.058	4.121ThrVal: 4.121 ± 0.059	0.746ThrTrp: 0.746 ± 0.025	1.056ThrTyr: 1.056 ± 0.031	0.001ThrXaa: 0.001 ± 0.001
Val	9.676ValAla: 9.676 ± 0.089	0.666ValCys: 0.666 ± 0.02	4.347ValAsp: 4.347 ± 0.054	4.048ValGlu: 4.048 ± 0.057	2.569ValPhe: 2.569 ± 0.043	5.745ValGly: 5.745 ± 0.06	1.532ValHis: 1.532 ± 0.029	3.065ValIle: 3.065 ± 0.052	2.468ValLys: 2.468 ± 0.044	8.098ValLeu: 8.098 ± 0.082	1.756ValMet: 1.756 ± 0.035	2.169ValAsn: 2.169 ± 0.039	3.801ValPro: 3.801 ± 0.047	2.972ValGln: 2.972 ± 0.048	4.957ValArg: 4.957 ± 0.06	3.929ValSer: 3.929 ± 0.052	3.971ValThr: 3.971 ± 0.053	5.975ValVal: 5.975 ± 0.081	1.048ValTrp: 1.048 ± 0.025	1.515ValTyr: 1.515 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.837TrpAla: 1.837 ± 0.033	0.174TrpCys: 0.174 ± 0.008	0.698TrpAsp: 0.698 ± 0.024	0.546TrpGlu: 0.546 ± 0.019	0.569TrpPhe: 0.569 ± 0.019	1.096TrpGly: 1.096 ± 0.026	0.364TrpHis: 0.364 ± 0.017	0.578TrpIle: 0.578 ± 0.022	0.475TrpLys: 0.475 ± 0.021	2.283TrpLeu: 2.283 ± 0.044	0.441TrpMet: 0.441 ± 0.015	0.426TrpAsn: 0.426 ± 0.017	0.889TrpPro: 0.889 ± 0.024	0.922TrpGln: 0.922 ± 0.026	1.592TrpArg: 1.592 ± 0.035	0.84TrpSer: 0.84 ± 0.027	0.87TrpThr: 0.87 ± 0.025	1.161TrpVal: 1.161 ± 0.028	0.352TrpTrp: 0.352 ± 0.015	0.303TrpTyr: 0.303 ± 0.013	0.001TrpXaa: 0.001 ± 0.0
Tyr	2.651TyrAla: 2.651 ± 0.041	0.197TyrCys: 0.197 ± 0.011	1.158TyrAsp: 1.158 ± 0.028	1.102TyrGlu: 1.102 ± 0.025	0.817TyrPhe: 0.817 ± 0.024	1.877TyrGly: 1.877 ± 0.032	0.419TyrHis: 0.419 ± 0.015	0.675TyrIle: 0.675 ± 0.021	0.6TyrLys: 0.6 ± 0.025	2.199TyrLeu: 2.199 ± 0.038	0.342TyrMet: 0.342 ± 0.013	0.571TyrAsn: 0.571 ± 0.021	1.0TyrPro: 1.0 ± 0.027	0.767TyrGln: 0.767 ± 0.021	1.724TyrArg: 1.724 ± 0.033	1.116TyrSer: 1.116 ± 0.03	1.146TyrThr: 1.146 ± 0.027	1.555TyrVal: 1.555 ± 0.03	0.401TyrTrp: 0.401 ± 0.015	0.547TyrTyr: 0.547 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 5103 proteins (1742311 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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