Amino acid dipepetide frequency for Escherichia phage slur01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.435AlaAla: 10.435 ± 1.5
0.973AlaCys: 0.973 ± 0.234
4.271AlaAsp: 4.271 ± 0.596
6.055AlaGlu: 6.055 ± 0.769
3.298AlaPhe: 3.298 ± 0.637
5.353AlaGly: 5.353 ± 0.599
1.46AlaHis: 1.46 ± 0.227
4.92AlaIle: 4.92 ± 0.626
6.326AlaLys: 6.326 ± 0.697
7.137AlaLeu: 7.137 ± 0.638
3.136AlaMet: 3.136 ± 0.546
3.568AlaAsn: 3.568 ± 0.49
3.785AlaPro: 3.785 ± 0.769
4.92AlaGln: 4.92 ± 0.912
4.109AlaArg: 4.109 ± 0.579
5.028AlaSer: 5.028 ± 0.731
6.055AlaThr: 6.055 ± 0.863
5.407AlaVal: 5.407 ± 0.515
1.406AlaTrp: 1.406 ± 0.237
2.541AlaTyr: 2.541 ± 0.401
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.192
0.487CysCys: 0.487 ± 0.211
1.081CysAsp: 1.081 ± 0.264
1.027CysGlu: 1.027 ± 0.273
0.433CysPhe: 0.433 ± 0.145
1.298CysGly: 1.298 ± 0.364
0.595CysHis: 0.595 ± 0.2
0.919CysIle: 0.919 ± 0.211
0.811CysLys: 0.811 ± 0.236
0.919CysLeu: 0.919 ± 0.257
0.216CysMet: 0.216 ± 0.109
0.811CysAsn: 0.811 ± 0.225
0.703CysPro: 0.703 ± 0.188
0.541CysGln: 0.541 ± 0.18
0.649CysArg: 0.649 ± 0.191
0.541CysSer: 0.541 ± 0.198
0.541CysThr: 0.541 ± 0.17
1.027CysVal: 1.027 ± 0.244
0.27CysTrp: 0.27 ± 0.136
0.649CysTyr: 0.649 ± 0.216
0.0CysXaa: 0.0 ± 0.0
Asp
5.515AspAla: 5.515 ± 0.517
0.703AspCys: 0.703 ± 0.203
2.703AspAsp: 2.703 ± 0.497
4.001AspGlu: 4.001 ± 0.384
2.271AspPhe: 2.271 ± 0.356
4.704AspGly: 4.704 ± 0.551
1.406AspHis: 1.406 ± 0.31
3.947AspIle: 3.947 ± 0.491
3.731AspLys: 3.731 ± 0.501
4.109AspLeu: 4.109 ± 0.422
1.244AspMet: 1.244 ± 0.231
2.595AspAsn: 2.595 ± 0.407
2.487AspPro: 2.487 ± 0.353
1.73AspGln: 1.73 ± 0.3
2.433AspArg: 2.433 ± 0.397
2.92AspSer: 2.92 ± 0.376
2.811AspThr: 2.811 ± 0.437
3.785AspVal: 3.785 ± 0.321
1.081AspTrp: 1.081 ± 0.211
2.217AspTyr: 2.217 ± 0.377
0.0AspXaa: 0.0 ± 0.0
Glu
7.083GluAla: 7.083 ± 0.662
0.919GluCys: 0.919 ± 0.272
3.947GluAsp: 3.947 ± 0.527
4.325GluGlu: 4.325 ± 0.6
2.163GluPhe: 2.163 ± 0.309
4.271GluGly: 4.271 ± 0.477
1.135GluHis: 1.135 ± 0.365
4.217GluIle: 4.217 ± 0.569
3.46GluLys: 3.46 ± 0.516
5.353GluLeu: 5.353 ± 0.583
2.163GluMet: 2.163 ± 0.359
2.974GluAsn: 2.974 ± 0.452
2.271GluPro: 2.271 ± 0.469
3.568GluGln: 3.568 ± 0.454
3.893GluArg: 3.893 ± 0.453
2.757GluSer: 2.757 ± 0.358
3.244GluThr: 3.244 ± 0.498
3.893GluVal: 3.893 ± 0.412
1.189GluTrp: 1.189 ± 0.248
2.054GluTyr: 2.054 ± 0.371
0.0GluXaa: 0.0 ± 0.0
Phe
3.082PheAla: 3.082 ± 0.401
0.541PheCys: 0.541 ± 0.211
2.325PheAsp: 2.325 ± 0.395
2.0PheGlu: 2.0 ± 0.311
1.244PhePhe: 1.244 ± 0.218
2.379PheGly: 2.379 ± 0.437
0.757PheHis: 0.757 ± 0.189
2.271PheIle: 2.271 ± 0.426
2.487PheLys: 2.487 ± 0.33
2.487PheLeu: 2.487 ± 0.364
1.081PheMet: 1.081 ± 0.269
2.109PheAsn: 2.109 ± 0.302
1.46PhePro: 1.46 ± 0.335
0.973PheGln: 0.973 ± 0.257
1.838PheArg: 1.838 ± 0.293
2.109PheSer: 2.109 ± 0.33
2.054PheThr: 2.054 ± 0.357
2.92PheVal: 2.92 ± 0.466
0.433PheTrp: 0.433 ± 0.134
0.973PheTyr: 0.973 ± 0.295
0.0PheXaa: 0.0 ± 0.0
Gly
5.839GlyAla: 5.839 ± 0.757
1.406GlyCys: 1.406 ± 0.3
3.352GlyAsp: 3.352 ± 0.367
4.596GlyGlu: 4.596 ± 0.466
3.298GlyPhe: 3.298 ± 0.576
4.866GlyGly: 4.866 ± 0.668
1.027GlyHis: 1.027 ± 0.282
3.568GlyIle: 3.568 ± 0.442
4.812GlyLys: 4.812 ± 0.486
5.569GlyLeu: 5.569 ± 0.561
2.054GlyMet: 2.054 ± 0.39
3.514GlyAsn: 3.514 ± 0.586
2.163GlyPro: 2.163 ± 0.279
2.703GlyGln: 2.703 ± 0.377
3.514GlyArg: 3.514 ± 0.389
4.487GlySer: 4.487 ± 0.51
4.866GlyThr: 4.866 ± 0.801
6.163GlyVal: 6.163 ± 0.549
1.298GlyTrp: 1.298 ± 0.226
3.19GlyTyr: 3.19 ± 0.372
0.0GlyXaa: 0.0 ± 0.0
His
1.46HisAla: 1.46 ± 0.278
0.324HisCys: 0.324 ± 0.121
0.919HisAsp: 0.919 ± 0.205
1.244HisGlu: 1.244 ± 0.34
0.757HisPhe: 0.757 ± 0.248
1.568HisGly: 1.568 ± 0.313
0.595HisHis: 0.595 ± 0.178
0.973HisIle: 0.973 ± 0.208
0.865HisLys: 0.865 ± 0.197
1.298HisLeu: 1.298 ± 0.273
0.541HisMet: 0.541 ± 0.175
1.027HisAsn: 1.027 ± 0.355
0.811HisPro: 0.811 ± 0.26
0.919HisGln: 0.919 ± 0.23
0.703HisArg: 0.703 ± 0.201
1.027HisSer: 1.027 ± 0.261
1.135HisThr: 1.135 ± 0.232
1.298HisVal: 1.298 ± 0.26
0.378HisTrp: 0.378 ± 0.156
0.649HisTyr: 0.649 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
5.731IleAla: 5.731 ± 0.566
0.865IleCys: 0.865 ± 0.233
3.785IleAsp: 3.785 ± 0.478
4.001IleGlu: 4.001 ± 0.499
1.622IlePhe: 1.622 ± 0.317
3.622IleGly: 3.622 ± 0.4
0.919IleHis: 0.919 ± 0.221
3.785IleIle: 3.785 ± 0.439
3.406IleLys: 3.406 ± 0.528
3.352IleLeu: 3.352 ± 0.379
1.784IleMet: 1.784 ± 0.34
3.947IleAsn: 3.947 ± 0.491
2.757IlePro: 2.757 ± 0.299
1.676IleGln: 1.676 ± 0.294
3.406IleArg: 3.406 ± 0.419
3.514IleSer: 3.514 ± 0.376
3.244IleThr: 3.244 ± 0.407
4.542IleVal: 4.542 ± 0.58
0.703IleTrp: 0.703 ± 0.195
1.73IleTyr: 1.73 ± 0.285
0.0IleXaa: 0.0 ± 0.0
Lys
5.947LysAla: 5.947 ± 0.785
0.703LysCys: 0.703 ± 0.228
4.325LysAsp: 4.325 ± 0.521
4.65LysGlu: 4.65 ± 0.624
2.865LysPhe: 2.865 ± 0.432
4.271LysGly: 4.271 ± 0.492
1.406LysHis: 1.406 ± 0.268
3.893LysIle: 3.893 ± 0.448
3.839LysLys: 3.839 ± 0.521
4.758LysLeu: 4.758 ± 0.617
1.46LysMet: 1.46 ± 0.366
2.811LysAsn: 2.811 ± 0.391
2.703LysPro: 2.703 ± 0.388
2.109LysGln: 2.109 ± 0.384
3.028LysArg: 3.028 ± 0.437
3.136LysSer: 3.136 ± 0.395
3.406LysThr: 3.406 ± 0.431
4.704LysVal: 4.704 ± 0.522
0.811LysTrp: 0.811 ± 0.251
2.433LysTyr: 2.433 ± 0.385
0.0LysXaa: 0.0 ± 0.0
Leu
6.218LeuAla: 6.218 ± 0.892
1.081LeuCys: 1.081 ± 0.249
4.596LeuAsp: 4.596 ± 0.47
4.596LeuGlu: 4.596 ± 0.541
1.892LeuPhe: 1.892 ± 0.345
5.028LeuGly: 5.028 ± 0.521
1.568LeuHis: 1.568 ± 0.297
4.271LeuIle: 4.271 ± 0.589
4.487LeuLys: 4.487 ± 0.474
4.325LeuLeu: 4.325 ± 0.487
1.892LeuMet: 1.892 ± 0.322
5.461LeuAsn: 5.461 ± 0.669
4.217LeuPro: 4.217 ± 0.6
3.298LeuGln: 3.298 ± 0.418
4.596LeuArg: 4.596 ± 0.453
4.596LeuSer: 4.596 ± 0.457
5.082LeuThr: 5.082 ± 0.493
4.163LeuVal: 4.163 ± 0.44
0.649LeuTrp: 0.649 ± 0.196
2.325LeuTyr: 2.325 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.325MetAla: 2.325 ± 0.398
0.378MetCys: 0.378 ± 0.141
1.081MetAsp: 1.081 ± 0.224
2.054MetGlu: 2.054 ± 0.385
0.649MetPhe: 0.649 ± 0.15
2.379MetGly: 2.379 ± 0.455
0.433MetHis: 0.433 ± 0.165
1.568MetIle: 1.568 ± 0.3
2.325MetLys: 2.325 ± 0.344
2.379MetLeu: 2.379 ± 0.379
0.595MetMet: 0.595 ± 0.17
0.973MetAsn: 0.973 ± 0.21
1.135MetPro: 1.135 ± 0.217
1.46MetGln: 1.46 ± 0.358
1.73MetArg: 1.73 ± 0.35
2.271MetSer: 2.271 ± 0.389
1.892MetThr: 1.892 ± 0.332
1.298MetVal: 1.298 ± 0.208
0.216MetTrp: 0.216 ± 0.114
1.135MetTyr: 1.135 ± 0.245
0.0MetXaa: 0.0 ± 0.0
Asn
3.785AsnAla: 3.785 ± 0.567
0.703AsnCys: 0.703 ± 0.233
2.974AsnAsp: 2.974 ± 0.341
2.865AsnGlu: 2.865 ± 0.381
1.892AsnPhe: 1.892 ± 0.299
5.461AsnGly: 5.461 ± 0.561
1.298AsnHis: 1.298 ± 0.29
2.487AsnIle: 2.487 ± 0.38
3.298AsnLys: 3.298 ± 0.501
3.676AsnLeu: 3.676 ± 0.446
1.298AsnMet: 1.298 ± 0.252
3.028AsnAsn: 3.028 ± 0.492
2.487AsnPro: 2.487 ± 0.445
1.406AsnGln: 1.406 ± 0.216
2.487AsnArg: 2.487 ± 0.395
1.73AsnSer: 1.73 ± 0.305
3.028AsnThr: 3.028 ± 0.385
3.947AsnVal: 3.947 ± 0.533
1.027AsnTrp: 1.027 ± 0.219
1.676AsnTyr: 1.676 ± 0.326
0.0AsnXaa: 0.0 ± 0.0
Pro
3.352ProAla: 3.352 ± 0.596
0.433ProCys: 0.433 ± 0.15
2.595ProAsp: 2.595 ± 0.524
3.676ProGlu: 3.676 ± 0.502
1.622ProPhe: 1.622 ± 0.39
2.92ProGly: 2.92 ± 0.34
0.757ProHis: 0.757 ± 0.2
1.676ProIle: 1.676 ± 0.279
2.379ProLys: 2.379 ± 0.412
2.92ProLeu: 2.92 ± 0.478
1.838ProMet: 1.838 ± 0.358
1.838ProAsn: 1.838 ± 0.36
1.784ProPro: 1.784 ± 0.375
1.838ProGln: 1.838 ± 0.463
1.568ProArg: 1.568 ± 0.337
3.19ProSer: 3.19 ± 0.439
2.109ProThr: 2.109 ± 0.327
3.406ProVal: 3.406 ± 0.679
0.865ProTrp: 0.865 ± 0.326
1.46ProTyr: 1.46 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
4.109GlnAla: 4.109 ± 0.808
0.487GlnCys: 0.487 ± 0.145
2.649GlnAsp: 2.649 ± 0.391
2.487GlnGlu: 2.487 ± 0.403
1.244GlnPhe: 1.244 ± 0.262
3.136GlnGly: 3.136 ± 0.401
0.703GlnHis: 0.703 ± 0.182
2.433GlnIle: 2.433 ± 0.345
2.325GlnLys: 2.325 ± 0.454
3.785GlnLeu: 3.785 ± 0.42
1.298GlnMet: 1.298 ± 0.29
1.568GlnAsn: 1.568 ± 0.275
2.109GlnPro: 2.109 ± 0.78
3.406GlnGln: 3.406 ± 1.489
2.541GlnArg: 2.541 ± 0.377
1.946GlnSer: 1.946 ± 0.377
2.379GlnThr: 2.379 ± 0.367
2.757GlnVal: 2.757 ± 0.357
0.757GlnTrp: 0.757 ± 0.188
1.081GlnTyr: 1.081 ± 0.313
0.0GlnXaa: 0.0 ± 0.0
Arg
3.514ArgAla: 3.514 ± 0.47
0.595ArgCys: 0.595 ± 0.184
2.649ArgAsp: 2.649 ± 0.413
3.028ArgGlu: 3.028 ± 0.529
1.622ArgPhe: 1.622 ± 0.277
3.622ArgGly: 3.622 ± 0.368
0.811ArgHis: 0.811 ± 0.222
3.19ArgIle: 3.19 ± 0.39
3.244ArgLys: 3.244 ± 0.393
4.433ArgLeu: 4.433 ± 0.431
1.568ArgMet: 1.568 ± 0.313
2.163ArgAsn: 2.163 ± 0.327
2.217ArgPro: 2.217 ± 0.392
1.946ArgGln: 1.946 ± 0.28
2.379ArgArg: 2.379 ± 0.363
2.487ArgSer: 2.487 ± 0.383
2.811ArgThr: 2.811 ± 0.342
3.947ArgVal: 3.947 ± 0.387
1.244ArgTrp: 1.244 ± 0.295
2.325ArgTyr: 2.325 ± 0.341
0.0ArgXaa: 0.0 ± 0.0
Ser
5.407SerAla: 5.407 ± 0.583
0.865SerCys: 0.865 ± 0.237
2.595SerAsp: 2.595 ± 0.393
3.514SerGlu: 3.514 ± 0.457
2.325SerPhe: 2.325 ± 0.372
5.028SerGly: 5.028 ± 0.651
0.757SerHis: 0.757 ± 0.183
2.811SerIle: 2.811 ± 0.425
3.298SerLys: 3.298 ± 0.497
4.217SerLeu: 4.217 ± 0.594
1.514SerMet: 1.514 ± 0.303
2.703SerAsn: 2.703 ± 0.369
1.784SerPro: 1.784 ± 0.259
2.271SerGln: 2.271 ± 0.473
2.649SerArg: 2.649 ± 0.407
2.865SerSer: 2.865 ± 0.377
3.028SerThr: 3.028 ± 0.374
3.893SerVal: 3.893 ± 0.427
0.919SerTrp: 0.919 ± 0.285
2.271SerTyr: 2.271 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
5.461ThrAla: 5.461 ± 0.887
0.811ThrCys: 0.811 ± 0.237
3.136ThrAsp: 3.136 ± 0.375
2.92ThrGlu: 2.92 ± 0.346
2.379ThrPhe: 2.379 ± 0.397
4.704ThrGly: 4.704 ± 0.56
0.811ThrHis: 0.811 ± 0.254
4.109ThrIle: 4.109 ± 0.599
3.298ThrLys: 3.298 ± 0.423
4.325ThrLeu: 4.325 ± 0.438
1.892ThrMet: 1.892 ± 0.31
3.082ThrAsn: 3.082 ± 0.405
2.649ThrPro: 2.649 ± 0.44
2.811ThrGln: 2.811 ± 0.347
2.271ThrArg: 2.271 ± 0.336
3.947ThrSer: 3.947 ± 0.652
4.001ThrThr: 4.001 ± 0.676
4.704ThrVal: 4.704 ± 0.773
1.135ThrTrp: 1.135 ± 0.272
2.0ThrTyr: 2.0 ± 0.429
0.0ThrXaa: 0.0 ± 0.0
Val
5.785ValAla: 5.785 ± 0.607
1.189ValCys: 1.189 ± 0.256
3.947ValAsp: 3.947 ± 0.403
4.92ValGlu: 4.92 ± 0.595
2.271ValPhe: 2.271 ± 0.408
3.731ValGly: 3.731 ± 0.437
1.244ValHis: 1.244 ± 0.216
4.704ValIle: 4.704 ± 0.511
5.244ValLys: 5.244 ± 0.49
5.082ValLeu: 5.082 ± 0.499
1.568ValMet: 1.568 ± 0.295
3.676ValAsn: 3.676 ± 0.401
2.595ValPro: 2.595 ± 0.366
3.46ValGln: 3.46 ± 0.505
3.082ValArg: 3.082 ± 0.491
3.244ValSer: 3.244 ± 0.367
5.677ValThr: 5.677 ± 0.591
5.677ValVal: 5.677 ± 0.701
0.703ValTrp: 0.703 ± 0.191
3.19ValTyr: 3.19 ± 0.439
0.0ValXaa: 0.0 ± 0.0
Trp
1.189TrpAla: 1.189 ± 0.26
0.216TrpCys: 0.216 ± 0.107
1.189TrpAsp: 1.189 ± 0.229
0.757TrpGlu: 0.757 ± 0.255
0.487TrpPhe: 0.487 ± 0.142
1.027TrpGly: 1.027 ± 0.246
0.162TrpHis: 0.162 ± 0.079
0.541TrpIle: 0.541 ± 0.165
1.298TrpLys: 1.298 ± 0.309
1.514TrpLeu: 1.514 ± 0.227
0.433TrpMet: 0.433 ± 0.134
0.703TrpAsn: 0.703 ± 0.19
0.649TrpPro: 0.649 ± 0.18
0.865TrpGln: 0.865 ± 0.235
0.973TrpArg: 0.973 ± 0.217
0.757TrpSer: 0.757 ± 0.224
1.027TrpThr: 1.027 ± 0.264
0.973TrpVal: 0.973 ± 0.214
0.324TrpTrp: 0.324 ± 0.119
0.703TrpTyr: 0.703 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.298TyrAla: 3.298 ± 0.437
0.487TyrCys: 0.487 ± 0.175
2.271TyrAsp: 2.271 ± 0.353
2.163TyrGlu: 2.163 ± 0.378
1.189TyrPhe: 1.189 ± 0.241
2.92TyrGly: 2.92 ± 0.333
0.649TyrHis: 0.649 ± 0.19
2.109TyrIle: 2.109 ± 0.318
2.325TyrLys: 2.325 ± 0.311
2.703TyrLeu: 2.703 ± 0.372
0.649TyrMet: 0.649 ± 0.198
2.0TyrAsn: 2.0 ± 0.281
1.46TyrPro: 1.46 ± 0.339
1.298TyrGln: 1.298 ± 0.26
1.946TyrArg: 1.946 ± 0.349
2.217TyrSer: 2.217 ± 0.304
2.054TyrThr: 2.054 ± 0.355
2.487TyrVal: 2.487 ± 0.354
0.378TyrTrp: 0.378 ± 0.155
1.514TyrTyr: 1.514 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 90 proteins (18497 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski