Amino acid dipepetide frequency for candidate division TM6 bacterium Zodletone_IIa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.982AlaAla: 6.982 ± 0.616
0.714AlaCys: 0.714 ± 0.164
3.669AlaAsp: 3.669 ± 0.312
4.157AlaGlu: 4.157 ± 0.377
4.092AlaPhe: 4.092 ± 0.351
5.326AlaGly: 5.326 ± 0.46
1.137AlaHis: 1.137 ± 0.193
6.202AlaIle: 6.202 ± 0.459
5.066AlaLys: 5.066 ± 0.457
7.339AlaLeu: 7.339 ± 0.512
1.786AlaMet: 1.786 ± 0.253
4.059AlaAsn: 4.059 ± 0.355
2.046AlaPro: 2.046 ± 0.257
2.825AlaGln: 2.825 ± 0.288
2.63AlaArg: 2.63 ± 0.291
4.092AlaSer: 4.092 ± 0.404
4.254AlaThr: 4.254 ± 0.412
5.098AlaVal: 5.098 ± 0.477
0.714AlaTrp: 0.714 ± 0.147
2.695AlaTyr: 2.695 ± 0.267
0.0AlaXaa: 0.0 ± 0.0
Cys
0.747CysAla: 0.747 ± 0.176
0.357CysCys: 0.357 ± 0.105
0.487CysAsp: 0.487 ± 0.125
0.292CysGlu: 0.292 ± 0.086
0.617CysPhe: 0.617 ± 0.169
0.585CysGly: 0.585 ± 0.133
0.13CysHis: 0.13 ± 0.065
0.812CysIle: 0.812 ± 0.163
0.585CysLys: 0.585 ± 0.102
1.072CysLeu: 1.072 ± 0.181
0.195CysMet: 0.195 ± 0.072
0.357CysAsn: 0.357 ± 0.1
0.617CysPro: 0.617 ± 0.145
0.325CysGln: 0.325 ± 0.093
0.455CysArg: 0.455 ± 0.114
0.682CysSer: 0.682 ± 0.143
0.52CysThr: 0.52 ± 0.156
0.682CysVal: 0.682 ± 0.146
0.065CysTrp: 0.065 ± 0.05
0.422CysTyr: 0.422 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
3.897AspAla: 3.897 ± 0.383
0.487AspCys: 0.487 ± 0.124
2.371AspAsp: 2.371 ± 0.339
2.825AspGlu: 2.825 ± 0.303
2.793AspPhe: 2.793 ± 0.242
3.669AspGly: 3.669 ± 0.459
1.169AspHis: 1.169 ± 0.194
3.377AspIle: 3.377 ± 0.346
3.345AspLys: 3.345 ± 0.35
4.189AspLeu: 4.189 ± 0.377
0.974AspMet: 0.974 ± 0.158
1.948AspAsn: 1.948 ± 0.256
1.494AspPro: 1.494 ± 0.216
1.851AspGln: 1.851 ± 0.24
1.754AspArg: 1.754 ± 0.242
2.923AspSer: 2.923 ± 0.36
2.565AspThr: 2.565 ± 0.318
3.182AspVal: 3.182 ± 0.295
0.649AspTrp: 0.649 ± 0.145
2.468AspTyr: 2.468 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
3.767GluAla: 3.767 ± 0.341
0.227GluCys: 0.227 ± 0.068
2.435GluAsp: 2.435 ± 0.325
2.89GluGlu: 2.89 ± 0.4
1.883GluPhe: 1.883 ± 0.304
3.247GluGly: 3.247 ± 0.339
1.201GluHis: 1.201 ± 0.178
4.286GluIle: 4.286 ± 0.439
5.163GluLys: 5.163 ± 0.488
5.455GluLeu: 5.455 ± 0.496
1.818GluMet: 1.818 ± 0.264
2.825GluAsn: 2.825 ± 0.312
1.689GluPro: 1.689 ± 0.239
2.63GluGln: 2.63 ± 0.303
2.176GluArg: 2.176 ± 0.279
2.728GluSer: 2.728 ± 0.268
2.793GluThr: 2.793 ± 0.309
3.604GluVal: 3.604 ± 0.372
0.714GluTrp: 0.714 ± 0.141
2.111GluTyr: 2.111 ± 0.28
0.0GluXaa: 0.0 ± 0.0
Phe
2.987PheAla: 2.987 ± 0.316
0.585PheCys: 0.585 ± 0.148
2.728PheAsp: 2.728 ± 0.299
1.883PheGlu: 1.883 ± 0.263
2.728PhePhe: 2.728 ± 0.375
2.663PheGly: 2.663 ± 0.3
1.299PheHis: 1.299 ± 0.189
4.384PheIle: 4.384 ± 0.477
2.955PheLys: 2.955 ± 0.317
6.137PheLeu: 6.137 ± 0.454
1.104PheMet: 1.104 ± 0.187
2.858PheAsn: 2.858 ± 0.311
2.241PhePro: 2.241 ± 0.285
1.429PheGln: 1.429 ± 0.2
1.851PheArg: 1.851 ± 0.268
4.059PheSer: 4.059 ± 0.365
3.15PheThr: 3.15 ± 0.349
3.215PheVal: 3.215 ± 0.322
0.649PheTrp: 0.649 ± 0.135
1.981PheTyr: 1.981 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
4.351GlyAla: 4.351 ± 0.4
0.649GlyCys: 0.649 ± 0.127
2.143GlyAsp: 2.143 ± 0.249
2.987GlyGlu: 2.987 ± 0.302
3.345GlyPhe: 3.345 ± 0.399
4.092GlyGly: 4.092 ± 0.475
1.299GlyHis: 1.299 ± 0.212
5.813GlyIle: 5.813 ± 0.498
5.813GlyLys: 5.813 ± 0.506
6.495GlyLeu: 6.495 ± 0.344
2.013GlyMet: 2.013 ± 0.219
4.059GlyAsn: 4.059 ± 0.324
1.981GlyPro: 1.981 ± 0.25
2.111GlyGln: 2.111 ± 0.274
2.598GlyArg: 2.598 ± 0.292
3.215GlySer: 3.215 ± 0.371
3.637GlyThr: 3.637 ± 0.388
4.676GlyVal: 4.676 ± 0.434
0.682GlyTrp: 0.682 ± 0.149
2.793GlyTyr: 2.793 ± 0.308
0.0GlyXaa: 0.0 ± 0.0
His
1.559HisAla: 1.559 ± 0.222
0.13HisCys: 0.13 ± 0.065
0.812HisAsp: 0.812 ± 0.165
1.072HisGlu: 1.072 ± 0.191
1.169HisPhe: 1.169 ± 0.217
1.364HisGly: 1.364 ± 0.202
0.617HisHis: 0.617 ± 0.139
1.526HisIle: 1.526 ± 0.219
1.039HisLys: 1.039 ± 0.197
2.5HisLeu: 2.5 ± 0.323
0.585HisMet: 0.585 ± 0.151
1.039HisAsn: 1.039 ± 0.217
1.169HisPro: 1.169 ± 0.217
1.072HisGln: 1.072 ± 0.172
1.007HisArg: 1.007 ± 0.165
1.169HisSer: 1.169 ± 0.206
1.299HisThr: 1.299 ± 0.196
1.072HisVal: 1.072 ± 0.182
0.422HisTrp: 0.422 ± 0.112
1.039HisTyr: 1.039 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
6.365IleAla: 6.365 ± 0.517
0.812IleCys: 0.812 ± 0.172
4.579IleAsp: 4.579 ± 0.38
4.124IleGlu: 4.124 ± 0.409
3.929IlePhe: 3.929 ± 0.46
4.254IleGly: 4.254 ± 0.435
1.786IleHis: 1.786 ± 0.237
7.079IleIle: 7.079 ± 0.648
4.741IleLys: 4.741 ± 0.367
7.534IleLeu: 7.534 ± 0.5
1.754IleMet: 1.754 ± 0.284
4.611IleAsn: 4.611 ± 0.354
4.157IlePro: 4.157 ± 0.365
3.442IleGln: 3.442 ± 0.312
2.955IleArg: 2.955 ± 0.323
4.449IleSer: 4.449 ± 0.336
4.416IleThr: 4.416 ± 0.334
5.39IleVal: 5.39 ± 0.413
0.617IleTrp: 0.617 ± 0.166
2.987IleTyr: 2.987 ± 0.42
0.0IleXaa: 0.0 ± 0.0
Lys
5.326LysAla: 5.326 ± 0.469
0.52LysCys: 0.52 ± 0.159
4.449LysAsp: 4.449 ± 0.443
5.001LysGlu: 5.001 ± 0.462
2.143LysPhe: 2.143 ± 0.25
4.514LysGly: 4.514 ± 0.405
1.364LysHis: 1.364 ± 0.187
6.007LysIle: 6.007 ± 0.446
6.462LysLys: 6.462 ± 0.639
5.943LysLeu: 5.943 ± 0.468
2.468LysMet: 2.468 ± 0.298
3.897LysAsn: 3.897 ± 0.364
3.02LysPro: 3.02 ± 0.335
3.117LysGln: 3.117 ± 0.341
2.923LysArg: 2.923 ± 0.331
3.767LysSer: 3.767 ± 0.362
3.637LysThr: 3.637 ± 0.346
4.059LysVal: 4.059 ± 0.367
0.844LysTrp: 0.844 ± 0.178
1.851LysTyr: 1.851 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
7.664LeuAla: 7.664 ± 0.505
0.909LeuCys: 0.909 ± 0.167
3.864LeuAsp: 3.864 ± 0.386
4.416LeuGlu: 4.416 ± 0.401
5.65LeuPhe: 5.65 ± 0.487
6.072LeuGly: 6.072 ± 0.54
1.883LeuHis: 1.883 ± 0.251
7.826LeuIle: 7.826 ± 0.557
7.209LeuLys: 7.209 ± 0.534
11.43LeuLeu: 11.43 ± 0.726
2.89LeuMet: 2.89 ± 0.291
4.157LeuAsn: 4.157 ± 0.43
5.001LeuPro: 5.001 ± 0.39
5.39LeuGln: 5.39 ± 0.43
3.215LeuArg: 3.215 ± 0.302
7.112LeuSer: 7.112 ± 0.565
5.39LeuThr: 5.39 ± 0.416
6.3LeuVal: 6.3 ± 0.448
1.429LeuTrp: 1.429 ± 0.25
3.247LeuTyr: 3.247 ± 0.331
0.0LeuXaa: 0.0 ± 0.0
Met
2.468MetAla: 2.468 ± 0.293
0.162MetCys: 0.162 ± 0.069
0.974MetAsp: 0.974 ± 0.178
1.266MetGlu: 1.266 ± 0.203
1.169MetPhe: 1.169 ± 0.243
2.176MetGly: 2.176 ± 0.277
0.812MetHis: 0.812 ± 0.163
1.948MetIle: 1.948 ± 0.277
2.111MetLys: 2.111 ± 0.264
2.338MetLeu: 2.338 ± 0.251
0.585MetMet: 0.585 ± 0.136
1.754MetAsn: 1.754 ± 0.256
1.299MetPro: 1.299 ± 0.212
0.909MetGln: 0.909 ± 0.171
0.974MetArg: 0.974 ± 0.162
1.559MetSer: 1.559 ± 0.197
1.266MetThr: 1.266 ± 0.208
1.559MetVal: 1.559 ± 0.229
0.13MetTrp: 0.13 ± 0.059
0.649MetTyr: 0.649 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
2.923AsnAla: 2.923 ± 0.302
0.487AsnCys: 0.487 ± 0.139
2.435AsnAsp: 2.435 ± 0.288
3.117AsnGlu: 3.117 ± 0.352
2.371AsnPhe: 2.371 ± 0.252
3.767AsnGly: 3.767 ± 0.351
1.331AsnHis: 1.331 ± 0.189
4.059AsnIle: 4.059 ± 0.411
3.442AsnLys: 3.442 ± 0.338
4.741AsnLeu: 4.741 ± 0.426
1.461AsnMet: 1.461 ± 0.21
3.247AsnAsn: 3.247 ± 0.328
2.046AsnPro: 2.046 ± 0.288
1.916AsnGln: 1.916 ± 0.244
1.559AsnArg: 1.559 ± 0.182
3.572AsnSer: 3.572 ± 0.355
2.371AsnThr: 2.371 ± 0.301
2.793AsnVal: 2.793 ± 0.341
0.682AsnTrp: 0.682 ± 0.161
2.078AsnTyr: 2.078 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
3.41ProAla: 3.41 ± 0.379
0.455ProCys: 0.455 ± 0.121
2.5ProAsp: 2.5 ± 0.253
2.565ProGlu: 2.565 ± 0.326
2.533ProPhe: 2.533 ± 0.324
3.182ProGly: 3.182 ± 0.334
0.747ProHis: 0.747 ± 0.144
2.76ProIle: 2.76 ± 0.272
2.241ProLys: 2.241 ± 0.262
4.806ProLeu: 4.806 ± 0.31
0.877ProMet: 0.877 ± 0.163
1.461ProAsn: 1.461 ± 0.197
1.299ProPro: 1.299 ± 0.247
1.624ProGln: 1.624 ± 0.186
1.169ProArg: 1.169 ± 0.179
2.371ProSer: 2.371 ± 0.264
2.046ProThr: 2.046 ± 0.23
3.929ProVal: 3.929 ± 0.349
0.292ProTrp: 0.292 ± 0.115
1.201ProTyr: 1.201 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
2.825GlnAla: 2.825 ± 0.259
0.39GlnCys: 0.39 ± 0.106
1.526GlnAsp: 1.526 ± 0.21
2.63GlnGlu: 2.63 ± 0.286
2.306GlnPhe: 2.306 ± 0.213
1.883GlnGly: 1.883 ± 0.248
0.844GlnHis: 0.844 ± 0.16
3.15GlnIle: 3.15 ± 0.349
2.793GlnLys: 2.793 ± 0.346
4.221GlnLeu: 4.221 ± 0.364
1.007GlnMet: 1.007 ± 0.168
1.883GlnAsn: 1.883 ± 0.244
1.624GlnPro: 1.624 ± 0.241
2.793GlnGln: 2.793 ± 0.364
1.754GlnArg: 1.754 ± 0.241
2.371GlnSer: 2.371 ± 0.267
2.533GlnThr: 2.533 ± 0.311
2.306GlnVal: 2.306 ± 0.277
0.455GlnTrp: 0.455 ± 0.135
1.721GlnTyr: 1.721 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
2.273ArgAla: 2.273 ± 0.284
0.195ArgCys: 0.195 ± 0.077
1.364ArgAsp: 1.364 ± 0.233
2.598ArgGlu: 2.598 ± 0.328
2.338ArgPhe: 2.338 ± 0.284
2.241ArgGly: 2.241 ± 0.273
0.649ArgHis: 0.649 ± 0.141
3.215ArgIle: 3.215 ± 0.327
2.89ArgLys: 2.89 ± 0.298
3.572ArgLeu: 3.572 ± 0.373
1.104ArgMet: 1.104 ± 0.217
1.559ArgAsn: 1.559 ± 0.216
1.396ArgPro: 1.396 ± 0.241
1.883ArgGln: 1.883 ± 0.218
1.851ArgArg: 1.851 ± 0.281
2.078ArgSer: 2.078 ± 0.257
1.689ArgThr: 1.689 ± 0.232
2.111ArgVal: 2.111 ± 0.284
0.779ArgTrp: 0.779 ± 0.158
1.624ArgTyr: 1.624 ± 0.223
0.0ArgXaa: 0.0 ± 0.0
Ser
4.741SerAla: 4.741 ± 0.41
0.39SerCys: 0.39 ± 0.138
2.143SerAsp: 2.143 ± 0.307
2.858SerGlu: 2.858 ± 0.318
3.345SerPhe: 3.345 ± 0.399
4.286SerGly: 4.286 ± 0.408
1.494SerHis: 1.494 ± 0.219
4.968SerIle: 4.968 ± 0.396
3.962SerLys: 3.962 ± 0.329
6.397SerLeu: 6.397 ± 0.462
1.461SerMet: 1.461 ± 0.207
2.435SerAsn: 2.435 ± 0.313
2.176SerPro: 2.176 ± 0.232
1.916SerGln: 1.916 ± 0.253
2.371SerArg: 2.371 ± 0.335
3.994SerSer: 3.994 ± 0.423
3.312SerThr: 3.312 ± 0.337
4.027SerVal: 4.027 ± 0.368
0.812SerTrp: 0.812 ± 0.177
2.046SerTyr: 2.046 ± 0.239
0.0SerXaa: 0.0 ± 0.0
Thr
4.319ThrAla: 4.319 ± 0.392
0.714ThrCys: 0.714 ± 0.165
2.825ThrAsp: 2.825 ± 0.319
2.858ThrGlu: 2.858 ± 0.309
2.403ThrPhe: 2.403 ± 0.269
4.481ThrGly: 4.481 ± 0.31
1.331ThrHis: 1.331 ± 0.216
4.611ThrIle: 4.611 ± 0.318
2.987ThrLys: 2.987 ± 0.302
5.196ThrLeu: 5.196 ± 0.405
0.974ThrMet: 0.974 ± 0.143
2.663ThrAsn: 2.663 ± 0.316
2.89ThrPro: 2.89 ± 0.238
1.266ThrGln: 1.266 ± 0.164
1.754ThrArg: 1.754 ± 0.223
2.76ThrSer: 2.76 ± 0.281
3.052ThrThr: 3.052 ± 0.287
3.215ThrVal: 3.215 ± 0.26
0.617ThrTrp: 0.617 ± 0.131
2.176ThrTyr: 2.176 ± 0.221
0.0ThrXaa: 0.0 ± 0.0
Val
5.001ValAla: 5.001 ± 0.389
1.201ValCys: 1.201 ± 0.194
3.442ValAsp: 3.442 ± 0.341
3.41ValGlu: 3.41 ± 0.348
2.663ValPhe: 2.663 ± 0.248
3.767ValGly: 3.767 ± 0.341
1.396ValHis: 1.396 ± 0.207
5.196ValIle: 5.196 ± 0.45
4.676ValLys: 4.676 ± 0.45
6.56ValLeu: 6.56 ± 0.495
2.013ValMet: 2.013 ± 0.255
2.793ValAsn: 2.793 ± 0.268
3.215ValPro: 3.215 ± 0.301
2.306ValGln: 2.306 ± 0.262
2.468ValArg: 2.468 ± 0.305
3.734ValSer: 3.734 ± 0.351
3.085ValThr: 3.085 ± 0.288
4.968ValVal: 4.968 ± 0.559
0.877ValTrp: 0.877 ± 0.198
2.111ValTyr: 2.111 ± 0.273
0.0ValXaa: 0.0 ± 0.0
Trp
0.747TrpAla: 0.747 ± 0.169
0.065TrpCys: 0.065 ± 0.046
0.552TrpAsp: 0.552 ± 0.157
0.552TrpGlu: 0.552 ± 0.117
0.909TrpPhe: 0.909 ± 0.154
0.812TrpGly: 0.812 ± 0.145
0.39TrpHis: 0.39 ± 0.111
0.942TrpIle: 0.942 ± 0.155
1.039TrpLys: 1.039 ± 0.185
1.494TrpLeu: 1.494 ± 0.205
0.227TrpMet: 0.227 ± 0.074
0.357TrpAsn: 0.357 ± 0.103
0.325TrpPro: 0.325 ± 0.101
0.552TrpGln: 0.552 ± 0.111
0.357TrpArg: 0.357 ± 0.098
0.585TrpSer: 0.585 ± 0.149
0.455TrpThr: 0.455 ± 0.124
0.909TrpVal: 0.909 ± 0.176
0.292TrpTrp: 0.292 ± 0.103
0.812TrpTyr: 0.812 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.533TyrAla: 2.533 ± 0.223
0.552TyrCys: 0.552 ± 0.131
2.63TyrAsp: 2.63 ± 0.307
2.143TyrGlu: 2.143 ± 0.262
2.5TyrPhe: 2.5 ± 0.308
2.371TyrGly: 2.371 ± 0.269
0.779TyrHis: 0.779 ± 0.175
1.818TyrIle: 1.818 ± 0.228
2.793TyrLys: 2.793 ± 0.292
3.637TyrLeu: 3.637 ± 0.346
0.779TyrMet: 0.779 ± 0.152
2.435TyrAsn: 2.435 ± 0.293
1.786TyrPro: 1.786 ± 0.261
1.656TyrGln: 1.656 ± 0.268
1.624TyrArg: 1.624 ± 0.239
1.916TyrSer: 1.916 ± 0.253
1.624TyrThr: 1.624 ± 0.239
1.786TyrVal: 1.786 ± 0.224
0.649TyrTrp: 0.649 ± 0.125
2.013TyrTyr: 2.013 ± 0.272
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 174 proteins (30796 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski