Clostridium phage phiCD119 (strain Clostridium difficile/United States/Govind/2006) (Bacteriophage phiCD119)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Lubbockvirus; Clostridium virus phiCD119

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q24LI8|Q24LI8_BPPCD Uncharacterized protein OS=Clostridium phage phiCD119 (strain Clostridium difficile/United States/Govind/2006) OX=1283341 PE=4 SV=1
MM1 pKa = 7.37TEE3 pKa = 3.98ADD5 pKa = 4.29ILALTYY11 pKa = 9.75FCKK14 pKa = 8.47MTIRR18 pKa = 11.84RR19 pKa = 11.84CVSIKK24 pKa = 11.01NEE26 pKa = 4.21DD27 pKa = 3.39TGVTYY32 pKa = 10.67FNEE35 pKa = 4.05NVVIAEE41 pKa = 4.53DD42 pKa = 4.48VPCGLNGNIPNVIDD56 pKa = 3.59TDD58 pKa = 3.57ITNSISVFEE67 pKa = 4.51LYY69 pKa = 10.24CRR71 pKa = 11.84PEE73 pKa = 4.29IDD75 pKa = 4.13LQVGDD80 pKa = 4.57ILDD83 pKa = 3.55ITLEE87 pKa = 4.16NGNVEE92 pKa = 4.16TFIASKK98 pKa = 9.57PFPYY102 pKa = 10.04SSHH105 pKa = 6.25LQVNLTLKK113 pKa = 10.13EE114 pKa = 4.08RR115 pKa = 11.84YY116 pKa = 9.07

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q24LD8|Q24LD8_BPPCD Uncharacterized protein OS=Clostridium phage phiCD119 (strain Clostridium difficile/United States/Govind/2006) OX=1283341 PE=3 SV=1
MM1 pKa = 7.66PGVTNRR7 pKa = 11.84NCDD10 pKa = 3.22NSGVLVNAVLTPDD23 pKa = 3.77RR24 pKa = 11.84VNKK27 pKa = 9.29RR28 pKa = 11.84QNGRR32 pKa = 11.84RR33 pKa = 11.84IKK35 pKa = 10.59EE36 pKa = 3.93SGEE39 pKa = 4.16TMFTLTAQDD48 pKa = 2.81KK49 pKa = 11.14HH50 pKa = 7.34GILKK54 pKa = 10.77NGDD57 pKa = 2.95IRR59 pKa = 11.84RR60 pKa = 11.84LTPKK64 pKa = 10.13EE65 pKa = 3.94CFRR68 pKa = 11.84LQGFPDD74 pKa = 3.53KK75 pKa = 10.72YY76 pKa = 9.66YY77 pKa = 10.41EE78 pKa = 4.21RR79 pKa = 11.84AASVCSDD86 pKa = 3.19SQLYY90 pKa = 8.57KK91 pKa = 10.15QAGNAVTANVVYY103 pKa = 10.16EE104 pKa = 3.71IAKK107 pKa = 10.5RR108 pKa = 11.84MGG110 pKa = 3.05

Molecular weight: 12.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

13692

57

944

173.3

19.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.696 ± 0.474

1.578 ± 0.154

5.697 ± 0.329

8.596 ± 0.465

4.156 ± 0.196

5.565 ± 0.514

1.161 ± 0.139

8.625 ± 0.254

10.963 ± 0.416

8.523 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.264 ± 0.143

6.895 ± 0.262

2.023 ± 0.134

2.892 ± 0.186

3.812 ± 0.268

5.748 ± 0.228

5.843 ± 0.362

5.762 ± 0.223

0.964 ± 0.13

4.236 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski