Nocardioides psychrotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Nocardioidaceae; Nocardioides

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4410 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3ETW6|A0A1I3ETW6_9ACTN Glycine cleavage system T protein (Aminomethyltransferase) OS=Nocardioides psychrotolerans OX=1005945 GN=SAMN05216561_10462 PE=3 SV=1
MM1 pKa = 7.88KK2 pKa = 10.1LRR4 pKa = 11.84KK5 pKa = 9.49ASAITGSLATLPLVLSACGGGDD27 pKa = 2.79IAAEE31 pKa = 4.12EE32 pKa = 4.72KK33 pKa = 10.79EE34 pKa = 4.35NEE36 pKa = 4.37SQVAAAGDD44 pKa = 3.97CGEE47 pKa = 5.26LNMAVNPWVGFAADD61 pKa = 3.98AYY63 pKa = 10.61VVGHH67 pKa = 6.62LAEE70 pKa = 4.63TEE72 pKa = 4.13LGCTVNYY79 pKa = 10.25KK80 pKa = 9.93DD81 pKa = 4.83LKK83 pKa = 11.26EE84 pKa = 4.01EE85 pKa = 4.24VSWQGFGTGEE95 pKa = 3.93VDD97 pKa = 4.81VVIEE101 pKa = 4.31DD102 pKa = 3.39WGHH105 pKa = 6.55PDD107 pKa = 3.72LEE109 pKa = 4.21KK110 pKa = 10.87LYY112 pKa = 11.08FEE114 pKa = 5.18DD115 pKa = 4.24QGGDD119 pKa = 3.28GSAVEE124 pKa = 4.62MGPTGNVGIIGWYY137 pKa = 7.4VAPWMAEE144 pKa = 4.11EE145 pKa = 4.73YY146 pKa = 10.45PDD148 pKa = 3.36ITDD151 pKa = 3.48WEE153 pKa = 4.37NLNDD157 pKa = 3.8YY158 pKa = 11.19ADD160 pKa = 3.57MFEE163 pKa = 4.36TPEE166 pKa = 4.28SGGKK170 pKa = 9.66GQFLGADD177 pKa = 3.55PSYY180 pKa = 11.13VQFDD184 pKa = 3.69EE185 pKa = 6.34AIVANLGLDD194 pKa = 3.67FQVVFSGSEE203 pKa = 3.75AASIAAFEE211 pKa = 4.09KK212 pKa = 11.09AEE214 pKa = 4.1ANKK217 pKa = 9.58TPLIGYY223 pKa = 8.72FYY225 pKa = 9.88EE226 pKa = 4.53PQWFMSEE233 pKa = 4.13VPLVKK238 pKa = 10.64VNLPEE243 pKa = 4.16FTDD246 pKa = 4.18GCQDD250 pKa = 3.2VAQDD254 pKa = 3.72VACDD258 pKa = 3.73YY259 pKa = 11.4PEE261 pKa = 4.16TQLQKK266 pKa = 10.49IVATEE271 pKa = 4.01FAEE274 pKa = 4.48SGSPAVDD281 pKa = 3.3LVSNFQWTNDD291 pKa = 3.73DD292 pKa = 3.48QNLVAKK298 pKa = 9.25YY299 pKa = 9.88ISVDD303 pKa = 3.11GMSAEE308 pKa = 4.09EE309 pKa = 4.77AAATWVEE316 pKa = 4.08EE317 pKa = 4.18NPDD320 pKa = 3.53KK321 pKa = 11.53VEE323 pKa = 3.76AWMSS327 pKa = 3.4

Molecular weight: 35.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3MAZ9|A0A1I3MAZ9_9ACTN Uncharacterized protein OS=Nocardioides psychrotolerans OX=1005945 GN=SAMN05216561_11534 PE=4 SV=1
MM1 pKa = 7.6GFTFNRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 10.12RR10 pKa = 11.84LGRR13 pKa = 11.84NTLNVSKK20 pKa = 9.51TGASLSRR27 pKa = 11.84RR28 pKa = 11.84AGPVTLSTRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84GTVRR43 pKa = 11.84LGKK46 pKa = 10.68GLGFRR51 pKa = 11.84FKK53 pKa = 11.05LL54 pKa = 3.68

Molecular weight: 6.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4410

0

4410

1380600

29

2059

313.1

33.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.719 ± 0.045

0.762 ± 0.01

6.585 ± 0.031

5.718 ± 0.038

2.837 ± 0.02

9.149 ± 0.035

2.227 ± 0.02

3.588 ± 0.025

1.959 ± 0.027

10.28 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.9 ± 0.014

1.764 ± 0.019

5.571 ± 0.027

2.847 ± 0.018

7.412 ± 0.038

5.517 ± 0.025

6.318 ± 0.024

9.433 ± 0.039

1.489 ± 0.016

1.925 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski