Anaerobacillus alkaliphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anaerobacillus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4643 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q0VRJ2|A0A4Q0VRJ2_9BACI TlyA family RNA methyltransferase OS=Anaerobacillus alkaliphilus OX=1548597 GN=DS745_15010 PE=4 SV=1
MM1 pKa = 7.47TLLKK5 pKa = 10.41NINVNIMLAVVGIFAVTVFLFMLQSHH31 pKa = 6.34SVPVDD36 pKa = 3.44QVSEE40 pKa = 4.14EE41 pKa = 4.24VTVEE45 pKa = 4.01EE46 pKa = 4.84NGNEE50 pKa = 3.86ASKK53 pKa = 11.12SEE55 pKa = 4.04NGNHH59 pKa = 7.39DD60 pKa = 3.6EE61 pKa = 4.97TEE63 pKa = 4.3TNTNDD68 pKa = 3.33NGSEE72 pKa = 4.14EE73 pKa = 4.25NEE75 pKa = 4.55SNGSDD80 pKa = 3.03IEE82 pKa = 4.24EE83 pKa = 4.37ANEE86 pKa = 3.77NTNGQEE92 pKa = 4.2YY93 pKa = 10.4LWGLDD98 pKa = 3.09SADD101 pKa = 3.85RR102 pKa = 11.84VDD104 pKa = 4.48QSFYY108 pKa = 11.47DD109 pKa = 3.7CFVTNFGEE117 pKa = 4.3PVAFGRR123 pKa = 11.84YY124 pKa = 9.29LEE126 pKa = 4.54TKK128 pKa = 10.46DD129 pKa = 3.43GVSYY133 pKa = 10.86GLSEE137 pKa = 4.41EE138 pKa = 4.25EE139 pKa = 4.66VAFLQDD145 pKa = 3.5KK146 pKa = 9.77GIKK149 pKa = 9.53IIPIYY154 pKa = 10.8NHH156 pKa = 6.92FIDD159 pKa = 3.75GTTYY163 pKa = 10.5EE164 pKa = 4.6KK165 pKa = 10.79GVQEE169 pKa = 4.79AEE171 pKa = 4.01TAITYY176 pKa = 10.69AEE178 pKa = 4.68AIGVTEE184 pKa = 4.12GVYY187 pKa = 10.15IFANVEE193 pKa = 3.62PEE195 pKa = 4.05YY196 pKa = 10.59PIDD199 pKa = 4.72ADD201 pKa = 4.87FLLGWFEE208 pKa = 4.92TIQSSQYY215 pKa = 9.83KK216 pKa = 8.71PGVYY220 pKa = 10.25GIFGEE225 pKa = 4.59GRR227 pKa = 11.84EE228 pKa = 4.03LTAAFEE234 pKa = 4.18RR235 pKa = 11.84ALGNNEE241 pKa = 4.33AIGEE245 pKa = 4.15EE246 pKa = 4.18MAVWHH251 pKa = 6.38SSEE254 pKa = 4.28PYY256 pKa = 9.92IGITTKK262 pKa = 10.23EE263 pKa = 4.22NKK265 pKa = 9.81PEE267 pKa = 3.99FAPDD271 pKa = 3.12VPDD274 pKa = 4.37FVNVSIWQYY283 pKa = 11.28GIDD286 pKa = 5.05AEE288 pKa = 4.58TCNIDD293 pKa = 3.43TNLINSEE300 pKa = 4.11IMDD303 pKa = 4.39SLWW306 pKa = 4.04

Molecular weight: 34.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0VVH2|A0A4Q0VVH2_9BACI Uncharacterized protein OS=Anaerobacillus alkaliphilus OX=1548597 GN=DS745_06685 PE=4 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.45GFRR20 pKa = 11.84ARR22 pKa = 11.84MATANGRR29 pKa = 11.84KK30 pKa = 8.91VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.05GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4643

0

4643

1361674

15

3880

293.3

33.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.587 ± 0.046

0.716 ± 0.01

4.939 ± 0.024

7.554 ± 0.044

4.772 ± 0.028

6.729 ± 0.035

2.095 ± 0.016

7.993 ± 0.038

6.592 ± 0.034

9.994 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.019

4.596 ± 0.033

3.54 ± 0.021

3.755 ± 0.026

4.031 ± 0.025

5.907 ± 0.029

5.638 ± 0.029

7.334 ± 0.035

1.027 ± 0.014

3.602 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski