Tortoise microvirus 26

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W708|A0A4P8W708_9VIRU DNA pilot protein OS=Tortoise microvirus 26 OX=2583128 PE=4 SV=1
MM1 pKa = 7.62NKK3 pKa = 9.92RR4 pKa = 11.84IYY6 pKa = 10.45VIYY9 pKa = 9.16NTDD12 pKa = 3.37EE13 pKa = 4.19KK14 pKa = 11.26DD15 pKa = 3.71SNVFSARR22 pKa = 11.84STDD25 pKa = 3.37EE26 pKa = 4.14AFDD29 pKa = 3.66IAFQTIFDD37 pKa = 4.06TEE39 pKa = 4.12EE40 pKa = 3.6FDD42 pKa = 5.63EE43 pKa = 4.77FFNDD47 pKa = 3.19NGDD50 pKa = 3.66IVVTKK55 pKa = 10.61ANKK58 pKa = 10.2AYY60 pKa = 10.36FFEE63 pKa = 4.81CLKK66 pKa = 9.81GWKK69 pKa = 9.13MYY71 pKa = 10.73DD72 pKa = 3.22YY73 pKa = 11.45GKK75 pKa = 10.78YY76 pKa = 10.58DD77 pKa = 3.16SDD79 pKa = 3.68KK80 pKa = 11.21VLFSLTEE87 pKa = 3.85HH88 pKa = 7.16PNPIDD93 pKa = 3.38FNEE96 pKa = 4.03YY97 pKa = 10.86LKK99 pKa = 10.52ILQDD103 pKa = 3.58KK104 pKa = 10.66VVGLL108 pKa = 4.05

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9I5|A0A4P8W9I5_9VIRU Replication initiation protein OS=Tortoise microvirus 26 OX=2583128 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 10.04CSYY5 pKa = 10.5PFTVKK10 pKa = 10.6QNDD13 pKa = 2.84IHH15 pKa = 7.05GNNIYY20 pKa = 10.57QDD22 pKa = 3.95VACKK26 pKa = 10.16KK27 pKa = 10.37CYY29 pKa = 9.83SCRR32 pKa = 11.84KK33 pKa = 9.63SISQEE38 pKa = 3.09WFIRR42 pKa = 11.84LRR44 pKa = 11.84NEE46 pKa = 3.76LHH48 pKa = 5.25YY49 pKa = 10.72HH50 pKa = 4.88EE51 pKa = 5.99HH52 pKa = 6.33NSFVTLTYY60 pKa = 10.54SDD62 pKa = 3.76EE63 pKa = 4.33HH64 pKa = 7.76LPSDD68 pKa = 4.07FSISKK73 pKa = 10.4KK74 pKa = 10.4DD75 pKa = 3.26LQLFFKK81 pKa = 10.71RR82 pKa = 11.84LRR84 pKa = 11.84KK85 pKa = 9.32RR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.39SKK90 pKa = 10.78LKK92 pKa = 10.05YY93 pKa = 9.12FACGEE98 pKa = 4.29YY99 pKa = 10.64GSQSYY104 pKa = 9.43RR105 pKa = 11.84PHH107 pKa = 5.41YY108 pKa = 10.2HH109 pKa = 7.08LILFGVGLYY118 pKa = 10.41SEE120 pKa = 5.35DD121 pKa = 3.47KK122 pKa = 11.1LDD124 pKa = 5.48IIEE127 pKa = 4.21SWPYY131 pKa = 11.19ADD133 pKa = 3.67WSVPSILKK141 pKa = 10.54NSFGLVEE148 pKa = 4.84DD149 pKa = 4.04KK150 pKa = 10.91SIKK153 pKa = 9.49YY154 pKa = 8.64VCNYY158 pKa = 9.12VNKK161 pKa = 10.33SLNDD165 pKa = 3.61LEE167 pKa = 4.44MKK169 pKa = 10.39IIYY172 pKa = 9.15QDD174 pKa = 3.6NNMTPPFRR182 pKa = 11.84IMSHH186 pKa = 5.8GLGLLYY192 pKa = 10.31MEE194 pKa = 5.41DD195 pKa = 3.95NKK197 pKa = 11.05TKK199 pKa = 10.85LIDD202 pKa = 3.58EE203 pKa = 4.15QVYY206 pKa = 8.46RR207 pKa = 11.84HH208 pKa = 5.8QGKK211 pKa = 9.77EE212 pKa = 3.82YY213 pKa = 10.21NLPYY217 pKa = 10.53YY218 pKa = 10.12YY219 pKa = 10.31RR220 pKa = 11.84NKK222 pKa = 10.59LNIGIKK228 pKa = 9.43TSPKK232 pKa = 10.01FQDD235 pKa = 2.96KK236 pKa = 11.03VIDD239 pKa = 3.79YY240 pKa = 8.66RR241 pKa = 11.84AKK243 pKa = 9.94QIKK246 pKa = 9.8EE247 pKa = 3.47ITGIDD252 pKa = 3.5AEE254 pKa = 4.77GEE256 pKa = 3.87ALEE259 pKa = 4.15YY260 pKa = 11.26LEE262 pKa = 5.58LDD264 pKa = 3.19SFVRR268 pKa = 11.84VRR270 pKa = 11.84KK271 pKa = 8.8HH272 pKa = 4.53QRR274 pKa = 11.84SDD276 pKa = 3.08QRR278 pKa = 11.84EE279 pKa = 3.78RR280 pKa = 11.84SLKK283 pKa = 10.51AKK285 pKa = 10.28QSLFNKK291 pKa = 10.42DD292 pKa = 2.88KK293 pKa = 10.97FF294 pKa = 3.83

Molecular weight: 35.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1332

108

520

266.4

30.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.33 ± 1.224

0.976 ± 0.401

6.532 ± 0.641

5.405 ± 0.517

5.48 ± 0.712

6.006 ± 1.033

1.727 ± 0.608

5.931 ± 0.302

6.532 ± 1.418

8.108 ± 0.559

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.327 ± 0.275

6.006 ± 0.463

5.18 ± 0.905

5.856 ± 0.905

5.18 ± 0.428

7.808 ± 0.685

4.429 ± 1.071

4.429 ± 0.478

1.351 ± 0.446

5.405 ± 0.678
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski