Amino acid dipepetide frequency for Ralstonia phage Firinga

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.888AlaAla: 18.888 ± 2.027
0.911AlaCys: 0.911 ± 0.27
5.799AlaAsp: 5.799 ± 0.84
6.959AlaGlu: 6.959 ± 1.112
3.811AlaPhe: 3.811 ± 0.485
9.693AlaGly: 9.693 ± 1.072
1.491AlaHis: 1.491 ± 0.35
5.882AlaIle: 5.882 ± 0.726
5.716AlaLys: 5.716 ± 0.846
9.858AlaLeu: 9.858 ± 0.916
3.645AlaMet: 3.645 ± 0.583
6.13AlaAsn: 6.13 ± 0.855
5.799AlaPro: 5.799 ± 0.817
8.284AlaGln: 8.284 ± 1.585
6.627AlaArg: 6.627 ± 0.728
7.207AlaSer: 7.207 ± 0.837
8.201AlaThr: 8.201 ± 0.817
7.29AlaVal: 7.29 ± 0.779
1.905AlaTrp: 1.905 ± 0.371
3.811AlaTyr: 3.811 ± 0.534
0.0AlaXaa: 0.0 ± 0.0
Cys
0.746CysAla: 0.746 ± 0.264
0.0CysCys: 0.0 ± 0.0
0.414CysAsp: 0.414 ± 0.202
0.828CysGlu: 0.828 ± 0.318
0.0CysPhe: 0.0 ± 0.0
0.58CysGly: 0.58 ± 0.25
0.083CysHis: 0.083 ± 0.093
0.166CysIle: 0.166 ± 0.108
0.497CysLys: 0.497 ± 0.194
0.911CysLeu: 0.911 ± 0.257
0.166CysMet: 0.166 ± 0.108
0.663CysAsn: 0.663 ± 0.328
0.331CysPro: 0.331 ± 0.191
0.249CysGln: 0.249 ± 0.131
0.746CysArg: 0.746 ± 0.292
0.663CysSer: 0.663 ± 0.278
0.497CysThr: 0.497 ± 0.268
0.746CysVal: 0.746 ± 0.265
0.331CysTrp: 0.331 ± 0.153
0.414CysTyr: 0.414 ± 0.265
0.0CysXaa: 0.0 ± 0.0
Asp
6.71AspAla: 6.71 ± 1.072
0.911AspCys: 0.911 ± 0.309
4.142AspAsp: 4.142 ± 0.791
3.811AspGlu: 3.811 ± 0.714
1.988AspPhe: 1.988 ± 0.541
4.556AspGly: 4.556 ± 0.634
0.828AspHis: 0.828 ± 0.245
2.402AspIle: 2.402 ± 0.521
1.74AspLys: 1.74 ± 0.367
4.474AspLeu: 4.474 ± 0.738
1.16AspMet: 1.16 ± 0.346
2.32AspAsn: 2.32 ± 0.557
2.734AspPro: 2.734 ± 0.397
2.32AspGln: 2.32 ± 0.511
2.568AspArg: 2.568 ± 0.416
2.651AspSer: 2.651 ± 0.411
3.148AspThr: 3.148 ± 0.469
3.065AspVal: 3.065 ± 0.512
1.16AspTrp: 1.16 ± 0.217
1.243AspTyr: 1.243 ± 0.389
0.0AspXaa: 0.0 ± 0.0
Glu
5.633GluAla: 5.633 ± 0.862
0.497GluCys: 0.497 ± 0.171
2.485GluAsp: 2.485 ± 0.422
2.9GluGlu: 2.9 ± 0.652
1.243GluPhe: 1.243 ± 0.297
2.982GluGly: 2.982 ± 0.465
1.077GluHis: 1.077 ± 0.362
3.314GluIle: 3.314 ± 0.602
2.32GluLys: 2.32 ± 0.55
4.308GluLeu: 4.308 ± 0.576
1.657GluMet: 1.657 ± 0.403
1.74GluAsn: 1.74 ± 0.381
1.657GluPro: 1.657 ± 0.35
2.9GluGln: 2.9 ± 0.607
4.556GluArg: 4.556 ± 0.726
2.982GluSer: 2.982 ± 0.584
3.231GluThr: 3.231 ± 0.511
2.237GluVal: 2.237 ± 0.458
0.746GluTrp: 0.746 ± 0.299
1.325GluTyr: 1.325 ± 0.371
0.0GluXaa: 0.0 ± 0.0
Phe
3.065PheAla: 3.065 ± 0.512
0.083PheCys: 0.083 ± 0.069
2.32PheAsp: 2.32 ± 0.363
1.905PheGlu: 1.905 ± 0.46
0.746PhePhe: 0.746 ± 0.266
3.314PheGly: 3.314 ± 0.512
0.414PheHis: 0.414 ± 0.168
0.911PheIle: 0.911 ± 0.287
1.491PheLys: 1.491 ± 0.339
1.657PheLeu: 1.657 ± 0.335
0.497PheMet: 0.497 ± 0.147
1.74PheAsn: 1.74 ± 0.463
1.16PhePro: 1.16 ± 0.331
1.491PheGln: 1.491 ± 0.34
1.491PheArg: 1.491 ± 0.436
1.823PheSer: 1.823 ± 0.419
1.823PheThr: 1.823 ± 0.428
1.905PheVal: 1.905 ± 0.422
0.249PheTrp: 0.249 ± 0.164
0.58PheTyr: 0.58 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
11.018GlyAla: 11.018 ± 1.301
0.331GlyCys: 0.331 ± 0.174
4.142GlyAsp: 4.142 ± 0.64
3.562GlyGlu: 3.562 ± 0.459
2.071GlyPhe: 2.071 ± 0.415
7.29GlyGly: 7.29 ± 1.152
1.077GlyHis: 1.077 ± 0.259
3.065GlyIle: 3.065 ± 0.518
4.059GlyLys: 4.059 ± 0.775
6.959GlyLeu: 6.959 ± 0.721
1.823GlyMet: 1.823 ± 0.451
4.722GlyAsn: 4.722 ± 0.778
2.651GlyPro: 2.651 ± 0.452
4.391GlyGln: 4.391 ± 0.848
4.142GlyArg: 4.142 ± 0.568
5.633GlySer: 5.633 ± 0.835
6.876GlyThr: 6.876 ± 1.508
5.55GlyVal: 5.55 ± 0.774
1.243GlyTrp: 1.243 ± 0.357
2.9GlyTyr: 2.9 ± 0.406
0.0GlyXaa: 0.0 ± 0.0
His
1.408HisAla: 1.408 ± 0.313
0.083HisCys: 0.083 ± 0.08
0.746HisAsp: 0.746 ± 0.208
0.994HisGlu: 0.994 ± 0.35
0.414HisPhe: 0.414 ± 0.149
1.408HisGly: 1.408 ± 0.255
0.249HisHis: 0.249 ± 0.218
0.911HisIle: 0.911 ± 0.251
0.249HisLys: 0.249 ± 0.146
1.243HisLeu: 1.243 ± 0.346
0.911HisMet: 0.911 ± 0.248
0.249HisAsn: 0.249 ± 0.131
0.663HisPro: 0.663 ± 0.252
0.911HisGln: 0.911 ± 0.242
0.828HisArg: 0.828 ± 0.265
0.746HisSer: 0.746 ± 0.32
0.58HisThr: 0.58 ± 0.229
1.574HisVal: 1.574 ± 0.372
0.331HisTrp: 0.331 ± 0.208
0.331HisTyr: 0.331 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
7.456IleAla: 7.456 ± 0.618
0.746IleCys: 0.746 ± 0.288
3.148IleAsp: 3.148 ± 0.382
2.9IleGlu: 2.9 ± 0.465
1.574IlePhe: 1.574 ± 0.434
4.391IleGly: 4.391 ± 0.761
0.746IleHis: 0.746 ± 0.253
2.154IleIle: 2.154 ± 0.49
2.402IleLys: 2.402 ± 0.489
2.568IleLeu: 2.568 ± 0.445
0.828IleMet: 0.828 ± 0.23
1.905IleAsn: 1.905 ± 0.459
2.154IlePro: 2.154 ± 0.367
2.154IleGln: 2.154 ± 0.51
2.485IleArg: 2.485 ± 0.46
3.314IleSer: 3.314 ± 0.443
3.314IleThr: 3.314 ± 0.684
3.397IleVal: 3.397 ± 0.714
0.663IleTrp: 0.663 ± 0.286
1.243IleTyr: 1.243 ± 0.302
0.0IleXaa: 0.0 ± 0.0
Lys
4.474LysAla: 4.474 ± 0.697
0.497LysCys: 0.497 ± 0.203
2.237LysAsp: 2.237 ± 0.468
2.071LysGlu: 2.071 ± 0.596
1.16LysPhe: 1.16 ± 0.251
2.32LysGly: 2.32 ± 0.403
0.663LysHis: 0.663 ± 0.227
1.408LysIle: 1.408 ± 0.355
1.077LysLys: 1.077 ± 0.255
4.142LysLeu: 4.142 ± 0.612
1.077LysMet: 1.077 ± 0.275
1.574LysAsn: 1.574 ± 0.389
2.568LysPro: 2.568 ± 0.529
2.651LysGln: 2.651 ± 0.417
2.651LysArg: 2.651 ± 0.487
2.402LysSer: 2.402 ± 0.629
1.823LysThr: 1.823 ± 0.352
1.905LysVal: 1.905 ± 0.381
0.497LysTrp: 0.497 ± 0.182
0.663LysTyr: 0.663 ± 0.24
0.0LysXaa: 0.0 ± 0.0
Leu
8.781LeuAla: 8.781 ± 0.946
0.58LeuCys: 0.58 ± 0.218
3.976LeuAsp: 3.976 ± 0.651
3.314LeuGlu: 3.314 ± 0.678
1.74LeuPhe: 1.74 ± 0.39
7.622LeuGly: 7.622 ± 0.737
0.994LeuHis: 0.994 ± 0.286
3.397LeuIle: 3.397 ± 0.426
2.32LeuLys: 2.32 ± 0.355
5.219LeuLeu: 5.219 ± 0.722
2.154LeuMet: 2.154 ± 0.332
4.142LeuAsn: 4.142 ± 0.607
2.237LeuPro: 2.237 ± 0.455
4.971LeuGln: 4.971 ± 0.667
4.971LeuArg: 4.971 ± 0.811
5.799LeuSer: 5.799 ± 0.735
5.136LeuThr: 5.136 ± 0.668
4.142LeuVal: 4.142 ± 0.626
0.911LeuTrp: 0.911 ± 0.325
2.485LeuTyr: 2.485 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
2.817MetAla: 2.817 ± 0.41
0.331MetCys: 0.331 ± 0.157
1.408MetAsp: 1.408 ± 0.303
1.325MetGlu: 1.325 ± 0.25
0.58MetPhe: 0.58 ± 0.236
1.657MetGly: 1.657 ± 0.288
0.249MetHis: 0.249 ± 0.131
0.663MetIle: 0.663 ± 0.236
0.828MetLys: 0.828 ± 0.283
1.988MetLeu: 1.988 ± 0.345
0.166MetMet: 0.166 ± 0.126
0.911MetAsn: 0.911 ± 0.273
1.74MetPro: 1.74 ± 0.411
1.574MetGln: 1.574 ± 0.359
1.823MetArg: 1.823 ± 0.378
2.154MetSer: 2.154 ± 0.297
2.651MetThr: 2.651 ± 0.477
1.408MetVal: 1.408 ± 0.333
0.083MetTrp: 0.083 ± 0.082
0.331MetTyr: 0.331 ± 0.175
0.0MetXaa: 0.0 ± 0.0
Asn
7.125AsnAla: 7.125 ± 0.88
0.166AsnCys: 0.166 ± 0.16
2.071AsnAsp: 2.071 ± 0.384
1.657AsnGlu: 1.657 ± 0.306
1.077AsnPhe: 1.077 ± 0.336
5.053AsnGly: 5.053 ± 0.888
0.497AsnHis: 0.497 ± 0.248
2.237AsnIle: 2.237 ± 0.471
1.325AsnLys: 1.325 ± 0.36
2.9AsnLeu: 2.9 ± 0.619
0.828AsnMet: 0.828 ± 0.264
1.657AsnAsn: 1.657 ± 0.397
3.314AsnPro: 3.314 ± 0.624
3.148AsnGln: 3.148 ± 0.451
1.905AsnArg: 1.905 ± 0.408
3.479AsnSer: 3.479 ± 0.584
2.9AsnThr: 2.9 ± 0.554
2.817AsnVal: 2.817 ± 0.535
0.414AsnTrp: 0.414 ± 0.207
0.911AsnTyr: 0.911 ± 0.267
0.0AsnXaa: 0.0 ± 0.0
Pro
6.793ProAla: 6.793 ± 1.069
0.497ProCys: 0.497 ± 0.225
2.982ProAsp: 2.982 ± 0.6
2.568ProGlu: 2.568 ± 0.548
1.16ProPhe: 1.16 ± 0.306
3.148ProGly: 3.148 ± 0.685
0.746ProHis: 0.746 ± 0.348
2.817ProIle: 2.817 ± 0.476
1.657ProLys: 1.657 ± 0.427
2.982ProLeu: 2.982 ± 0.517
0.994ProMet: 0.994 ± 0.271
2.237ProAsn: 2.237 ± 0.388
1.408ProPro: 1.408 ± 0.218
2.402ProGln: 2.402 ± 0.471
2.071ProArg: 2.071 ± 0.459
3.728ProSer: 3.728 ± 0.634
3.065ProThr: 3.065 ± 0.677
2.9ProVal: 2.9 ± 0.425
0.58ProTrp: 0.58 ± 0.187
1.408ProTyr: 1.408 ± 0.322
0.0ProXaa: 0.0 ± 0.0
Gln
6.545GlnAla: 6.545 ± 1.143
0.911GlnCys: 0.911 ± 0.265
2.402GlnAsp: 2.402 ± 0.433
2.154GlnGlu: 2.154 ± 0.549
2.485GlnPhe: 2.485 ± 0.375
3.562GlnGly: 3.562 ± 0.599
1.16GlnHis: 1.16 ± 0.359
3.645GlnIle: 3.645 ± 0.409
1.408GlnLys: 1.408 ± 0.339
4.556GlnLeu: 4.556 ± 0.68
1.657GlnMet: 1.657 ± 0.344
2.071GlnAsn: 2.071 ± 0.518
3.231GlnPro: 3.231 ± 0.494
5.55GlnGln: 5.55 ± 1.287
3.397GlnArg: 3.397 ± 0.667
3.728GlnSer: 3.728 ± 0.654
3.562GlnThr: 3.562 ± 0.577
2.651GlnVal: 2.651 ± 0.451
0.911GlnTrp: 0.911 ± 0.216
1.574GlnTyr: 1.574 ± 0.379
0.0GlnXaa: 0.0 ± 0.0
Arg
5.882ArgAla: 5.882 ± 0.524
0.58ArgCys: 0.58 ± 0.181
3.148ArgAsp: 3.148 ± 0.595
3.231ArgGlu: 3.231 ± 0.795
1.657ArgPhe: 1.657 ± 0.314
3.231ArgGly: 3.231 ± 0.441
0.994ArgHis: 0.994 ± 0.381
3.479ArgIle: 3.479 ± 0.473
2.485ArgLys: 2.485 ± 0.484
4.059ArgLeu: 4.059 ± 0.603
1.657ArgMet: 1.657 ± 0.322
2.568ArgAsn: 2.568 ± 0.499
1.988ArgPro: 1.988 ± 0.398
3.065ArgGln: 3.065 ± 0.529
3.976ArgArg: 3.976 ± 0.82
3.231ArgSer: 3.231 ± 0.569
3.645ArgThr: 3.645 ± 0.367
5.219ArgVal: 5.219 ± 0.585
1.325ArgTrp: 1.325 ± 0.481
1.325ArgTyr: 1.325 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
10.024SerAla: 10.024 ± 1.277
0.249SerCys: 0.249 ± 0.153
2.982SerAsp: 2.982 ± 0.439
2.651SerGlu: 2.651 ± 0.546
1.74SerPhe: 1.74 ± 0.321
6.71SerGly: 6.71 ± 0.978
0.911SerHis: 0.911 ± 0.286
3.562SerIle: 3.562 ± 0.546
1.905SerLys: 1.905 ± 0.389
3.976SerLeu: 3.976 ± 0.701
1.823SerMet: 1.823 ± 0.319
2.9SerAsn: 2.9 ± 0.492
2.651SerPro: 2.651 ± 0.653
3.397SerGln: 3.397 ± 0.758
3.231SerArg: 3.231 ± 0.46
5.799SerSer: 5.799 ± 0.814
4.474SerThr: 4.474 ± 0.541
5.385SerVal: 5.385 ± 0.677
0.414SerTrp: 0.414 ± 0.163
1.823SerTyr: 1.823 ± 0.517
0.0SerXaa: 0.0 ± 0.0
Thr
8.45ThrAla: 8.45 ± 1.012
0.663ThrCys: 0.663 ± 0.248
3.148ThrAsp: 3.148 ± 0.554
2.568ThrGlu: 2.568 ± 0.416
1.74ThrPhe: 1.74 ± 0.437
7.456ThrGly: 7.456 ± 0.943
0.911ThrHis: 0.911 ± 0.235
4.225ThrIle: 4.225 ± 0.864
2.237ThrLys: 2.237 ± 0.483
5.385ThrLeu: 5.385 ± 0.782
1.491ThrMet: 1.491 ± 0.357
2.9ThrAsn: 2.9 ± 0.521
4.722ThrPro: 4.722 ± 0.584
3.148ThrGln: 3.148 ± 0.54
3.231ThrArg: 3.231 ± 0.399
3.479ThrSer: 3.479 ± 0.555
5.882ThrThr: 5.882 ± 0.865
5.385ThrVal: 5.385 ± 0.863
0.497ThrTrp: 0.497 ± 0.214
1.657ThrTyr: 1.657 ± 0.458
0.0ThrXaa: 0.0 ± 0.0
Val
8.119ValAla: 8.119 ± 0.911
0.414ValCys: 0.414 ± 0.178
3.314ValAsp: 3.314 ± 0.606
2.9ValGlu: 2.9 ± 0.671
2.237ValPhe: 2.237 ± 0.425
5.965ValGly: 5.965 ± 0.733
0.746ValHis: 0.746 ± 0.281
3.148ValIle: 3.148 ± 0.564
2.734ValLys: 2.734 ± 0.426
4.805ValLeu: 4.805 ± 0.511
1.325ValMet: 1.325 ± 0.36
3.148ValAsn: 3.148 ± 0.651
3.479ValPro: 3.479 ± 0.472
3.065ValGln: 3.065 ± 0.422
2.9ValArg: 2.9 ± 0.604
5.136ValSer: 5.136 ± 0.913
5.053ValThr: 5.053 ± 0.739
3.811ValVal: 3.811 ± 0.591
0.249ValTrp: 0.249 ± 0.157
1.491ValTyr: 1.491 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
0.994TrpAla: 0.994 ± 0.295
0.249TrpCys: 0.249 ± 0.158
0.994TrpAsp: 0.994 ± 0.357
0.497TrpGlu: 0.497 ± 0.266
0.746TrpPhe: 0.746 ± 0.261
0.828TrpGly: 0.828 ± 0.32
0.414TrpHis: 0.414 ± 0.201
1.077TrpIle: 1.077 ± 0.317
0.663TrpLys: 0.663 ± 0.321
1.243TrpLeu: 1.243 ± 0.34
0.249TrpMet: 0.249 ± 0.138
0.497TrpAsn: 0.497 ± 0.179
0.331TrpPro: 0.331 ± 0.211
0.497TrpGln: 0.497 ± 0.214
1.077TrpArg: 1.077 ± 0.342
0.663TrpSer: 0.663 ± 0.237
0.828TrpThr: 0.828 ± 0.267
0.911TrpVal: 0.911 ± 0.275
0.414TrpTrp: 0.414 ± 0.184
0.414TrpTyr: 0.414 ± 0.162
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.065TyrAla: 3.065 ± 0.504
0.331TyrCys: 0.331 ± 0.147
2.071TyrAsp: 2.071 ± 0.321
0.994TyrGlu: 0.994 ± 0.251
0.497TyrPhe: 0.497 ± 0.223
1.74TyrGly: 1.74 ± 0.328
0.58TyrHis: 0.58 ± 0.216
1.077TyrIle: 1.077 ± 0.284
0.746TyrLys: 0.746 ± 0.192
1.657TyrLeu: 1.657 ± 0.399
0.497TyrMet: 0.497 ± 0.153
1.408TyrAsn: 1.408 ± 0.396
1.325TyrPro: 1.325 ± 0.389
0.994TyrGln: 0.994 ± 0.291
2.071TyrArg: 2.071 ± 0.4
1.988TyrSer: 1.988 ± 0.412
2.485TyrThr: 2.485 ± 0.618
1.74TyrVal: 1.74 ± 0.37
0.663TyrTrp: 0.663 ± 0.254
0.497TyrTyr: 0.497 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12072 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski