Clavibacter phage CN1A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cinunavirus; Clavibacter virus CN1A

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PX26|U5PX26_9CAUD Uncharacterized protein OS=Clavibacter phage CN1A OX=1406793 GN=CN1A_28 PE=4 SV=1
MM1 pKa = 7.76QEE3 pKa = 3.67INTTLEE9 pKa = 3.53VDD11 pKa = 3.0NWGSEE16 pKa = 4.17VEE18 pKa = 4.19PVEE21 pKa = 5.07SEE23 pKa = 4.02SCGRR27 pKa = 11.84KK28 pKa = 8.03WLRR31 pKa = 11.84LEE33 pKa = 4.72NPDD36 pKa = 3.88HH37 pKa = 7.02VVDD40 pKa = 6.1LGDD43 pKa = 3.67EE44 pKa = 4.53DD45 pKa = 4.22GTLLIHH51 pKa = 7.01TYY53 pKa = 10.84TNVTHH58 pKa = 6.85EE59 pKa = 4.44GDD61 pKa = 3.72DD62 pKa = 4.1LEE64 pKa = 4.95VIGYY68 pKa = 7.64MSVYY72 pKa = 10.4SPEE75 pKa = 4.32AGQKK79 pKa = 10.17IIDD82 pKa = 4.37HH83 pKa = 6.48IKK85 pKa = 10.53EE86 pKa = 4.13VMGLL90 pKa = 3.53

Molecular weight: 10.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PXJ9|U5PXJ9_9CAUD HNH endonuclease OS=Clavibacter phage CN1A OX=1406793 GN=CN1A_74 PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 9.74IGRR6 pKa = 11.84KK7 pKa = 6.06TRR9 pKa = 11.84HH10 pKa = 5.11MLGRR14 pKa = 11.84PPIPLKK20 pKa = 10.83DD21 pKa = 3.69RR22 pKa = 11.84FWAKK26 pKa = 7.66VTKK29 pKa = 10.22TDD31 pKa = 4.22TCWLWTASKK40 pKa = 10.65NSKK43 pKa = 10.14GYY45 pKa = 10.94GRR47 pKa = 11.84IKK49 pKa = 9.86VGKK52 pKa = 9.39RR53 pKa = 11.84YY54 pKa = 9.32VAAHH58 pKa = 5.67RR59 pKa = 11.84VAYY62 pKa = 9.75QMEE65 pKa = 4.55VGPIPEE71 pKa = 4.66GLQLDD76 pKa = 4.0HH77 pKa = 6.87VKK79 pKa = 10.8ARR81 pKa = 11.84GYY83 pKa = 10.04VNRR86 pKa = 11.84HH87 pKa = 4.69CVKK90 pKa = 9.77PAHH93 pKa = 6.55LEE95 pKa = 3.92PVTARR100 pKa = 11.84VNIARR105 pKa = 11.84GNGIASQNARR115 pKa = 11.84KK116 pKa = 6.68THH118 pKa = 6.05CLRR121 pKa = 11.84DD122 pKa = 3.55HH123 pKa = 7.0EE124 pKa = 4.54FTKK127 pKa = 10.88EE128 pKa = 3.54NTYY131 pKa = 10.91LIGTRR136 pKa = 11.84RR137 pKa = 11.84QCKK140 pKa = 7.11TCARR144 pKa = 11.84LRR146 pKa = 11.84ASKK149 pKa = 10.75KK150 pKa = 8.86EE151 pKa = 3.88APKK154 pKa = 10.83

Molecular weight: 17.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16702

26

1436

214.1

23.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.675 ± 0.551

0.479 ± 0.107

6.376 ± 0.286

5.933 ± 0.338

3.413 ± 0.146

8.448 ± 0.291

1.658 ± 0.164

4.993 ± 0.171

4.7 ± 0.219

7.897 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.173

4.011 ± 0.15

4.365 ± 0.25

3.491 ± 0.198

6.161 ± 0.255

5.874 ± 0.273

7.041 ± 0.449

7.478 ± 0.288

1.808 ± 0.144

2.91 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski