bacterium 2013Arg42i

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1563 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4M1B7|A0A0S4M1B7_9BACT 50S ribosomal protein L27 OS=bacterium 2013Arg42i OX=1561003 GN=rpmA PE=3 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84VMGSSFSDD10 pKa = 3.45SVEE13 pKa = 3.9VFSAVRR19 pKa = 11.84DD20 pKa = 3.65DD21 pKa = 4.53HH22 pKa = 6.69EE23 pKa = 4.87QITSCGYY30 pKa = 9.52GANEE34 pKa = 3.95VVDD37 pKa = 6.12DD38 pKa = 4.63IFQGSDD44 pKa = 3.0VSSYY48 pKa = 11.33SLSGDD53 pKa = 3.07SSLRR57 pKa = 11.84EE58 pKa = 4.12SGCLSNNMFLIGFFFLVALYY78 pKa = 10.08ILVVVCLTIQSFSFDD93 pKa = 3.34NIAAVVIEE101 pKa = 4.57LGLFVGVIAAPMVMCLSTQCNGTNGRR127 pKa = 11.84LSIMSSSTDD136 pKa = 3.49LQPSVDD142 pKa = 4.27LQHH145 pKa = 7.22IDD147 pKa = 4.23ADD149 pKa = 4.46CDD151 pKa = 3.66DD152 pKa = 4.45TGNDD156 pKa = 2.7IDD158 pKa = 5.23ALVASISDD166 pKa = 3.69CLHH169 pKa = 6.94RR170 pKa = 11.84IEE172 pKa = 6.05DD173 pKa = 3.85SCGWANDD180 pKa = 2.9IVAYY184 pKa = 10.25GDD186 pKa = 3.48NSVDD190 pKa = 3.43LEE192 pKa = 4.29NYY194 pKa = 8.35VLHH197 pKa = 5.82MTNCLNRR204 pKa = 11.84MEE206 pKa = 4.26TRR208 pKa = 11.84ADD210 pKa = 3.46RR211 pKa = 11.84LLFFDD216 pKa = 4.27SEE218 pKa = 4.82EE219 pKa = 3.9ILTMINRR226 pKa = 11.84LDD228 pKa = 3.93LPQFPSNSLMALLTSDD244 pKa = 3.68KK245 pKa = 11.36

Molecular weight: 26.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4M6P0|A0A0S4M6P0_9BACT Uncharacterized protein OS=bacterium 2013Arg42i OX=1561003 GN=Ark11_0997 PE=4 SV=1
MM1 pKa = 7.6ALAKK5 pKa = 9.78TPHH8 pKa = 6.68LMTNMVMVRR17 pKa = 11.84TFVGVRR23 pKa = 11.84ALNTRR28 pKa = 11.84GLRR31 pKa = 11.84VIKK34 pKa = 10.56INVWGLL40 pKa = 3.13

Molecular weight: 4.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1563

0

1563

586317

26

2091

375.1

42.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.287 ± 0.076

1.87 ± 0.03

5.958 ± 0.051

5.939 ± 0.064

4.644 ± 0.046

5.422 ± 0.079

2.119 ± 0.026

8.129 ± 0.076

6.558 ± 0.099

9.579 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.306 ± 0.027

5.321 ± 0.072

3.503 ± 0.043

2.605 ± 0.043

5.064 ± 0.052

9.777 ± 0.075

4.704 ± 0.05

6.843 ± 0.077

0.831 ± 0.023

3.542 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski