Proteome-pI 2.0 - Proteome Isoelectric Point Database

Bergeriella denitrificans (Neisseria denitrificans)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2212 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A378UIF2|A0A378UIF2_BERDE Transcriptional regulator MraZ OS=Bergeriella denitrificans OX=494 GN=mraZ PE=3 SV=1

MM1 pKa = 6.82TTLFQPDD8 pKa = 4.38DD9 pKa = 4.51LNPNDD14 pKa = 4.23PAEE17 pKa = 4.1FLYY20 pKa = 10.37TLSAACFDD28 pKa = 3.81HH29 pKa = 7.03AAAIGDD35 pKa = 3.89EE36 pKa = 5.32DD37 pKa = 4.51LACLNDD43 pKa = 3.64EE44 pKa = 4.08QHH46 pKa = 7.05ALVAYY51 pKa = 9.72CYY53 pKa = 10.55LDD55 pKa = 3.37SQVQEE60 pKa = 4.19GGFVQLIAAGYY71 pKa = 9.2GEE73 pKa = 4.69YY74 pKa = 11.35VLMNPLADD82 pKa = 3.39SLRR85 pKa = 11.84RR86 pKa = 11.84WRR88 pKa = 11.84IKK90 pKa = 8.71PTPKK94 pKa = 10.54VLDD97 pKa = 3.7KK98 pKa = 11.5AKK100 pKa = 10.81ALYY103 pKa = 9.56QKK105 pKa = 10.9YY106 pKa = 10.02GGEE109 pKa = 3.98IEE111 pKa = 5.04ALAEE115 pKa = 4.02QEE117 pKa = 4.46TPVADD122 pKa = 3.49IRR124 pKa = 11.84AKK126 pKa = 10.42FADD129 pKa = 3.96FEE131 pKa = 4.43EE132 pKa = 5.69LDD134 pKa = 3.6GEE136 pKa = 4.55YY137 pKa = 10.97YY138 pKa = 10.41EE139 pKa = 5.98AADD142 pKa = 4.03EE143 pKa = 4.59DD144 pKa = 3.96MAAAVAYY151 pKa = 9.36VQANWARR158 pKa = 11.84FAEE161 pKa = 4.5IADD164 pKa = 3.69

Molecular weight: 18.2 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A378UHG6|A0A378UHG6_BERDE Argininosuccinate lyase OS=Bergeriella denitrificans OX=494 GN=argH PE=3 SV=1

MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2212	0	2212	668743	30	2648	302.3	33.2

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.671 ± 0.072	0.955 ± 0.018	5.298 ± 0.041	5.976 ± 0.057	3.917 ± 0.036	7.666 ± 0.062	2.191 ± 0.028	5.365 ± 0.039	4.841 ± 0.054	10.249 ± 0.076

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.553 ± 0.025	3.677 ± 0.047	4.357 ± 0.033	4.268 ± 0.041	5.56 ± 0.054	5.275 ± 0.034	5.141 ± 0.058	6.85 ± 0.048	1.305 ± 0.023	2.883 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski