Proteome-pI 2.0 - Proteome Isoelectric Point Database

Picobiliphyte sp. MS584-5 nanovirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Mulpavirales; Nanoviridae; Nanovirus; unclassified Nanovirus

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|F8RWS5|F8RWS5_9VIRU Rep protein OS=Picobiliphyte sp. MS584-5 nanovirus OX=1046893 PE=4 SV=1

MM1 pKa = 8.18DD2 pKa = 6.39DD3 pKa = 4.01DD4 pKa = 3.85RR5 pKa = 11.84ANRR8 pKa = 11.84AEE10 pKa = 4.11EE11 pKa = 4.12SGEE14 pKa = 4.09GNTVLSPPKK23 pKa = 10.15QGTQHH28 pKa = 5.95TFWCFTLNNYY38 pKa = 7.34TAEE41 pKa = 4.2QIEE44 pKa = 4.23HH45 pKa = 6.72LEE47 pKa = 3.94LVFKK51 pKa = 10.65HH52 pKa = 4.78EE53 pKa = 4.58CKK55 pKa = 9.75WYY57 pKa = 10.39LFQEE61 pKa = 4.52EE62 pKa = 4.46VGEE65 pKa = 4.37KK66 pKa = 10.42CGTPHH71 pKa = 6.97LQGQICLKK79 pKa = 9.15TRR81 pKa = 11.84MRR83 pKa = 11.84MTEE86 pKa = 4.22LKK88 pKa = 10.27RR89 pKa = 11.84IDD91 pKa = 3.76MKK93 pKa = 10.94ISWRR97 pKa = 11.84ATKK100 pKa = 10.44SCTASLEE107 pKa = 4.2YY108 pKa = 10.26CKK110 pKa = 10.59KK111 pKa = 10.72KK112 pKa = 9.26EE113 pKa = 3.98TSTGKK118 pKa = 9.91IYY120 pKa = 10.47YY121 pKa = 9.76HH122 pKa = 6.95GMDD125 pKa = 3.86VPDD128 pKa = 3.65QVDD131 pKa = 3.52LEE133 pKa = 4.52EE134 pKa = 4.86PYY136 pKa = 10.86GWQLDD141 pKa = 3.71ILNVLKK147 pKa = 10.61DD148 pKa = 3.62KK149 pKa = 10.03PHH151 pKa = 5.8NRR153 pKa = 11.84IIHH156 pKa = 6.06WYY158 pKa = 8.0WEE160 pKa = 3.94PTGGKK165 pKa = 10.22GKK167 pKa = 9.0TSLSKK172 pKa = 10.85YY173 pKa = 10.29LVVKK177 pKa = 10.47HH178 pKa = 6.48DD179 pKa = 4.06ALMLTGKK186 pKa = 10.44SNDD189 pKa = 3.53MYY191 pKa = 11.61HH192 pKa = 6.79MISKK196 pKa = 9.88FPKK199 pKa = 8.85KK200 pKa = 9.82RR201 pKa = 11.84KK202 pKa = 9.65LFIVDD207 pKa = 4.4CPRR210 pKa = 11.84SQQDD214 pKa = 3.68YY215 pKa = 9.67INYY218 pKa = 9.58GAIEE222 pKa = 4.07QIKK225 pKa = 10.52NGLIFSGKK233 pKa = 9.84YY234 pKa = 9.51EE235 pKa = 4.11GAQLVFNAPHH245 pKa = 6.23VIVFANEE252 pKa = 3.8PPDD255 pKa = 3.62ISKK258 pKa = 10.23MSLDD262 pKa = 2.93RR263 pKa = 11.84WNVVRR268 pKa = 11.84IAA270 pKa = 3.84

Molecular weight: 31.36 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|F8RWS5|F8RWS5_9VIRU Rep protein OS=Picobiliphyte sp. MS584-5 nanovirus OX=1046893 PE=4 SV=1

MM1 pKa = 8.18DD2 pKa = 6.39DD3 pKa = 4.01DD4 pKa = 3.85RR5 pKa = 11.84ANRR8 pKa = 11.84AEE10 pKa = 4.11EE11 pKa = 4.12SGEE14 pKa = 4.09GNTVLSPPKK23 pKa = 10.15QGTQHH28 pKa = 5.95TFWCFTLNNYY38 pKa = 7.34TAEE41 pKa = 4.2QIEE44 pKa = 4.23HH45 pKa = 6.72LEE47 pKa = 3.94LVFKK51 pKa = 10.65HH52 pKa = 4.78EE53 pKa = 4.58CKK55 pKa = 9.75WYY57 pKa = 10.39LFQEE61 pKa = 4.52EE62 pKa = 4.46VGEE65 pKa = 4.37KK66 pKa = 10.42CGTPHH71 pKa = 6.97LQGQICLKK79 pKa = 9.15TRR81 pKa = 11.84MRR83 pKa = 11.84MTEE86 pKa = 4.22LKK88 pKa = 10.27RR89 pKa = 11.84IDD91 pKa = 3.76MKK93 pKa = 10.94ISWRR97 pKa = 11.84ATKK100 pKa = 10.44SCTASLEE107 pKa = 4.2YY108 pKa = 10.26CKK110 pKa = 10.59KK111 pKa = 10.72KK112 pKa = 9.26EE113 pKa = 3.98TSTGKK118 pKa = 9.91IYY120 pKa = 10.47YY121 pKa = 9.76HH122 pKa = 6.95GMDD125 pKa = 3.86VPDD128 pKa = 3.65QVDD131 pKa = 3.52LEE133 pKa = 4.52EE134 pKa = 4.86PYY136 pKa = 10.86GWQLDD141 pKa = 3.71ILNVLKK147 pKa = 10.61DD148 pKa = 3.62KK149 pKa = 10.03PHH151 pKa = 5.8NRR153 pKa = 11.84IIHH156 pKa = 6.06WYY158 pKa = 8.0WEE160 pKa = 3.94PTGGKK165 pKa = 10.22GKK167 pKa = 9.0TSLSKK172 pKa = 10.85YY173 pKa = 10.29LVVKK177 pKa = 10.47HH178 pKa = 6.48DD179 pKa = 4.06ALMLTGKK186 pKa = 10.44SNDD189 pKa = 3.53MYY191 pKa = 11.61HH192 pKa = 6.79MISKK196 pKa = 9.88FPKK199 pKa = 8.85KK200 pKa = 9.82RR201 pKa = 11.84KK202 pKa = 9.65LFIVDD207 pKa = 4.4CPRR210 pKa = 11.84SQQDD214 pKa = 3.68YY215 pKa = 9.67INYY218 pKa = 9.58GAIEE222 pKa = 4.07QIKK225 pKa = 10.52NGLIFSGKK233 pKa = 9.84YY234 pKa = 9.51EE235 pKa = 4.11GAQLVFNAPHH245 pKa = 6.23VIVFANEE252 pKa = 3.8PPDD255 pKa = 3.62ISKK258 pKa = 10.23MSLDD262 pKa = 2.93RR263 pKa = 11.84WNVVRR268 pKa = 11.84IAA270 pKa = 3.84

Molecular weight: 31.36 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	270	270	270	270.0	31.36

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
4.074 ± 0.0	2.593 ± 0.0	5.556 ± 0.0	7.037 ± 0.0	3.333 ± 0.0	6.296 ± 0.0	3.704 ± 0.0	6.296 ± 0.0	9.63 ± 0.0	7.778 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
3.333 ± 0.0	4.444 ± 0.0	4.444 ± 0.0	4.444 ± 0.0	4.074 ± 0.0	5.185 ± 0.0	5.556 ± 0.0	5.185 ± 0.0	2.593 ± 0.0	4.444 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski