Amino acid dipepetide frequency for Erwinia phage Midgardsormr38

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.886AlaAla: 12.886 ± 2.153
0.889AlaCys: 0.889 ± 0.244
5.459AlaAsp: 5.459 ± 0.658
7.173AlaGlu: 7.173 ± 0.973
3.301AlaPhe: 3.301 ± 0.604
6.792AlaGly: 6.792 ± 0.756
1.333AlaHis: 1.333 ± 0.274
6.792AlaIle: 6.792 ± 0.655
5.713AlaLys: 5.713 ± 0.718
7.618AlaLeu: 7.618 ± 0.912
3.936AlaMet: 3.936 ± 0.527
3.999AlaAsn: 3.999 ± 0.786
2.031AlaPro: 2.031 ± 0.344
4.444AlaGln: 4.444 ± 0.998
5.459AlaArg: 5.459 ± 0.843
5.84AlaSer: 5.84 ± 0.939
6.094AlaThr: 6.094 ± 0.927
6.221AlaVal: 6.221 ± 0.719
1.904AlaTrp: 1.904 ± 0.363
3.301AlaTyr: 3.301 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
0.762CysAla: 0.762 ± 0.232
0.381CysCys: 0.381 ± 0.165
1.079CysAsp: 1.079 ± 0.262
0.762CysGlu: 0.762 ± 0.265
0.254CysPhe: 0.254 ± 0.144
1.46CysGly: 1.46 ± 0.316
0.317CysHis: 0.317 ± 0.184
0.571CysIle: 0.571 ± 0.229
0.762CysLys: 0.762 ± 0.235
0.952CysLeu: 0.952 ± 0.292
0.127CysMet: 0.127 ± 0.098
0.508CysAsn: 0.508 ± 0.194
0.444CysPro: 0.444 ± 0.224
0.571CysGln: 0.571 ± 0.192
1.016CysArg: 1.016 ± 0.307
1.206CysSer: 1.206 ± 0.36
0.635CysThr: 0.635 ± 0.21
0.571CysVal: 0.571 ± 0.204
0.381CysTrp: 0.381 ± 0.166
0.381CysTyr: 0.381 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
5.078AspAla: 5.078 ± 0.616
1.079AspCys: 1.079 ± 0.305
3.301AspAsp: 3.301 ± 0.479
4.634AspGlu: 4.634 ± 0.623
2.539AspPhe: 2.539 ± 0.47
4.698AspGly: 4.698 ± 0.6
0.825AspHis: 0.825 ± 0.214
3.936AspIle: 3.936 ± 0.42
3.047AspLys: 3.047 ± 0.475
3.872AspLeu: 3.872 ± 0.529
1.333AspMet: 1.333 ± 0.359
1.904AspAsn: 1.904 ± 0.424
1.904AspPro: 1.904 ± 0.305
2.095AspGln: 2.095 ± 0.399
3.682AspArg: 3.682 ± 0.459
3.618AspSer: 3.618 ± 0.458
3.364AspThr: 3.364 ± 0.497
3.618AspVal: 3.618 ± 0.469
1.143AspTrp: 1.143 ± 0.308
2.095AspTyr: 2.095 ± 0.46
0.0AspXaa: 0.0 ± 0.0
Glu
6.158GluAla: 6.158 ± 1.026
1.143GluCys: 1.143 ± 0.347
2.031GluAsp: 2.031 ± 0.349
3.491GluGlu: 3.491 ± 0.52
3.301GluPhe: 3.301 ± 0.464
4.507GluGly: 4.507 ± 0.483
1.587GluHis: 1.587 ± 0.362
4.761GluIle: 4.761 ± 0.576
4.19GluLys: 4.19 ± 0.711
5.332GluLeu: 5.332 ± 0.713
2.349GluMet: 2.349 ± 0.418
2.603GluAsn: 2.603 ± 0.444
1.714GluPro: 1.714 ± 0.339
4.507GluGln: 4.507 ± 0.548
4.38GluArg: 4.38 ± 0.592
3.872GluSer: 3.872 ± 0.599
2.92GluThr: 2.92 ± 0.46
4.126GluVal: 4.126 ± 0.566
1.016GluTrp: 1.016 ± 0.268
1.65GluTyr: 1.65 ± 0.389
0.0GluXaa: 0.0 ± 0.0
Phe
2.539PheAla: 2.539 ± 0.383
0.571PheCys: 0.571 ± 0.219
2.349PheAsp: 2.349 ± 0.425
2.349PheGlu: 2.349 ± 0.459
0.698PhePhe: 0.698 ± 0.208
2.285PheGly: 2.285 ± 0.366
0.571PheHis: 0.571 ± 0.194
2.285PheIle: 2.285 ± 0.43
1.968PheLys: 1.968 ± 0.375
1.587PheLeu: 1.587 ± 0.388
0.762PheMet: 0.762 ± 0.201
1.777PheAsn: 1.777 ± 0.362
1.016PhePro: 1.016 ± 0.314
0.698PheGln: 0.698 ± 0.249
2.476PheArg: 2.476 ± 0.492
2.222PheSer: 2.222 ± 0.346
2.793PheThr: 2.793 ± 0.43
1.587PheVal: 1.587 ± 0.337
0.381PheTrp: 0.381 ± 0.16
1.397PheTyr: 1.397 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
6.602GlyAla: 6.602 ± 0.66
0.571GlyCys: 0.571 ± 0.232
3.682GlyAsp: 3.682 ± 0.479
5.078GlyGlu: 5.078 ± 0.637
3.237GlyPhe: 3.237 ± 0.404
5.078GlyGly: 5.078 ± 0.745
0.952GlyHis: 0.952 ± 0.259
4.317GlyIle: 4.317 ± 0.524
4.951GlyLys: 4.951 ± 0.593
6.348GlyLeu: 6.348 ± 0.647
2.222GlyMet: 2.222 ± 0.402
2.92GlyAsn: 2.92 ± 0.431
1.46GlyPro: 1.46 ± 0.236
2.412GlyGln: 2.412 ± 0.335
4.571GlyArg: 4.571 ± 0.497
4.951GlySer: 4.951 ± 0.68
3.428GlyThr: 3.428 ± 0.665
5.142GlyVal: 5.142 ± 0.558
1.016GlyTrp: 1.016 ± 0.243
2.73GlyTyr: 2.73 ± 0.438
0.0GlyXaa: 0.0 ± 0.0
His
1.333HisAla: 1.333 ± 0.275
0.444HisCys: 0.444 ± 0.154
1.016HisAsp: 1.016 ± 0.237
1.143HisGlu: 1.143 ± 0.34
0.825HisPhe: 0.825 ± 0.207
1.079HisGly: 1.079 ± 0.243
0.508HisHis: 0.508 ± 0.187
1.206HisIle: 1.206 ± 0.255
0.698HisLys: 0.698 ± 0.256
1.27HisLeu: 1.27 ± 0.303
0.317HisMet: 0.317 ± 0.154
0.381HisAsn: 0.381 ± 0.177
0.825HisPro: 0.825 ± 0.271
0.698HisGln: 0.698 ± 0.226
0.635HisArg: 0.635 ± 0.242
0.571HisSer: 0.571 ± 0.2
0.952HisThr: 0.952 ± 0.209
0.698HisVal: 0.698 ± 0.197
0.317HisTrp: 0.317 ± 0.14
0.825HisTyr: 0.825 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
6.094IleAla: 6.094 ± 0.691
0.698IleCys: 0.698 ± 0.282
4.698IleAsp: 4.698 ± 0.589
4.571IleGlu: 4.571 ± 0.596
1.206IlePhe: 1.206 ± 0.287
4.507IleGly: 4.507 ± 0.608
1.206IleHis: 1.206 ± 0.289
3.999IleIle: 3.999 ± 0.61
4.19IleLys: 4.19 ± 0.66
3.111IleLeu: 3.111 ± 0.395
1.46IleMet: 1.46 ± 0.265
3.047IleAsn: 3.047 ± 0.428
2.984IlePro: 2.984 ± 0.462
2.539IleGln: 2.539 ± 0.371
3.301IleArg: 3.301 ± 0.323
4.698IleSer: 4.698 ± 0.642
4.761IleThr: 4.761 ± 0.476
2.857IleVal: 2.857 ± 0.419
1.016IleTrp: 1.016 ± 0.256
1.587IleTyr: 1.587 ± 0.349
0.0IleXaa: 0.0 ± 0.0
Lys
5.459LysAla: 5.459 ± 0.612
0.698LysCys: 0.698 ± 0.25
3.174LysAsp: 3.174 ± 0.467
4.19LysGlu: 4.19 ± 0.681
1.714LysPhe: 1.714 ± 0.346
3.618LysGly: 3.618 ± 0.635
1.143LysHis: 1.143 ± 0.335
2.73LysIle: 2.73 ± 0.404
3.618LysLys: 3.618 ± 0.786
4.571LysLeu: 4.571 ± 0.767
1.841LysMet: 1.841 ± 0.406
2.603LysAsn: 2.603 ± 0.415
2.666LysPro: 2.666 ± 0.436
2.73LysGln: 2.73 ± 0.471
3.111LysArg: 3.111 ± 0.378
4.634LysSer: 4.634 ± 0.522
3.491LysThr: 3.491 ± 0.434
4.444LysVal: 4.444 ± 0.635
1.27LysTrp: 1.27 ± 0.24
1.777LysTyr: 1.777 ± 0.331
0.0LysXaa: 0.0 ± 0.0
Leu
8.76LeuAla: 8.76 ± 1.045
1.397LeuCys: 1.397 ± 0.29
4.253LeuAsp: 4.253 ± 0.537
4.126LeuGlu: 4.126 ± 0.536
2.158LeuPhe: 2.158 ± 0.32
4.19LeuGly: 4.19 ± 0.472
1.333LeuHis: 1.333 ± 0.335
4.951LeuIle: 4.951 ± 0.542
4.126LeuLys: 4.126 ± 0.54
5.84LeuLeu: 5.84 ± 0.834
2.031LeuMet: 2.031 ± 0.428
3.364LeuAsn: 3.364 ± 0.546
2.92LeuPro: 2.92 ± 0.438
2.793LeuGln: 2.793 ± 0.399
5.142LeuArg: 5.142 ± 0.738
4.698LeuSer: 4.698 ± 0.581
6.285LeuThr: 6.285 ± 0.671
4.444LeuVal: 4.444 ± 0.582
1.016LeuTrp: 1.016 ± 0.251
2.158LeuTyr: 2.158 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
2.857MetAla: 2.857 ± 0.36
0.19MetCys: 0.19 ± 0.117
1.587MetAsp: 1.587 ± 0.375
1.143MetGlu: 1.143 ± 0.309
0.508MetPhe: 0.508 ± 0.153
2.412MetGly: 2.412 ± 0.393
0.317MetHis: 0.317 ± 0.132
1.143MetIle: 1.143 ± 0.285
2.539MetLys: 2.539 ± 0.495
1.65MetLeu: 1.65 ± 0.37
1.016MetMet: 1.016 ± 0.254
1.841MetAsn: 1.841 ± 0.34
1.079MetPro: 1.079 ± 0.35
1.524MetGln: 1.524 ± 0.352
1.841MetArg: 1.841 ± 0.334
2.539MetSer: 2.539 ± 0.49
2.031MetThr: 2.031 ± 0.374
1.65MetVal: 1.65 ± 0.34
0.254MetTrp: 0.254 ± 0.111
0.762MetTyr: 0.762 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
4.063AsnAla: 4.063 ± 0.699
0.635AsnCys: 0.635 ± 0.194
2.412AsnAsp: 2.412 ± 0.405
2.412AsnGlu: 2.412 ± 0.338
1.016AsnPhe: 1.016 ± 0.223
3.745AsnGly: 3.745 ± 0.555
0.762AsnHis: 0.762 ± 0.21
2.857AsnIle: 2.857 ± 0.411
2.285AsnLys: 2.285 ± 0.296
2.92AsnLeu: 2.92 ± 0.495
0.889AsnMet: 0.889 ± 0.249
2.285AsnAsn: 2.285 ± 0.412
1.777AsnPro: 1.777 ± 0.363
2.031AsnGln: 2.031 ± 0.426
1.841AsnArg: 1.841 ± 0.358
3.491AsnSer: 3.491 ± 0.452
2.222AsnThr: 2.222 ± 0.402
2.031AsnVal: 2.031 ± 0.324
0.317AsnTrp: 0.317 ± 0.126
1.714AsnTyr: 1.714 ± 0.311
0.0AsnXaa: 0.0 ± 0.0
Pro
4.317ProAla: 4.317 ± 0.535
0.571ProCys: 0.571 ± 0.228
2.539ProAsp: 2.539 ± 0.411
2.222ProGlu: 2.222 ± 0.362
1.143ProPhe: 1.143 ± 0.263
2.158ProGly: 2.158 ± 0.378
0.317ProHis: 0.317 ± 0.145
2.158ProIle: 2.158 ± 0.306
2.031ProLys: 2.031 ± 0.498
2.349ProLeu: 2.349 ± 0.422
1.333ProMet: 1.333 ± 0.347
1.397ProAsn: 1.397 ± 0.337
1.079ProPro: 1.079 ± 0.283
1.143ProGln: 1.143 ± 0.265
1.777ProArg: 1.777 ± 0.4
2.222ProSer: 2.222 ± 0.44
2.031ProThr: 2.031 ± 0.359
2.603ProVal: 2.603 ± 0.392
0.381ProTrp: 0.381 ± 0.168
1.016ProTyr: 1.016 ± 0.253
0.0ProXaa: 0.0 ± 0.0
Gln
4.951GlnAla: 4.951 ± 0.903
0.571GlnCys: 0.571 ± 0.217
1.714GlnAsp: 1.714 ± 0.344
2.222GlnGlu: 2.222 ± 0.338
1.524GlnPhe: 1.524 ± 0.49
2.603GlnGly: 2.603 ± 0.423
0.762GlnHis: 0.762 ± 0.195
2.285GlnIle: 2.285 ± 0.388
2.031GlnLys: 2.031 ± 0.346
3.364GlnLeu: 3.364 ± 0.545
1.27GlnMet: 1.27 ± 0.309
1.714GlnAsn: 1.714 ± 0.327
1.46GlnPro: 1.46 ± 0.328
2.793GlnGln: 2.793 ± 0.755
2.158GlnArg: 2.158 ± 0.454
2.793GlnSer: 2.793 ± 0.651
1.777GlnThr: 1.777 ± 0.325
3.682GlnVal: 3.682 ± 0.489
0.635GlnTrp: 0.635 ± 0.197
1.714GlnTyr: 1.714 ± 0.388
0.0GlnXaa: 0.0 ± 0.0
Arg
5.396ArgAla: 5.396 ± 0.661
0.825ArgCys: 0.825 ± 0.245
4.063ArgAsp: 4.063 ± 0.445
5.078ArgGlu: 5.078 ± 0.659
1.143ArgPhe: 1.143 ± 0.299
3.999ArgGly: 3.999 ± 0.403
1.016ArgHis: 1.016 ± 0.247
3.999ArgIle: 3.999 ± 0.626
4.317ArgLys: 4.317 ± 0.683
5.142ArgLeu: 5.142 ± 0.492
1.777ArgMet: 1.777 ± 0.382
2.031ArgAsn: 2.031 ± 0.396
1.904ArgPro: 1.904 ± 0.33
2.666ArgGln: 2.666 ± 0.417
4.824ArgArg: 4.824 ± 0.598
3.237ArgSer: 3.237 ± 0.462
3.174ArgThr: 3.174 ± 0.447
2.984ArgVal: 2.984 ± 0.522
0.571ArgTrp: 0.571 ± 0.187
1.714ArgTyr: 1.714 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
6.729SerAla: 6.729 ± 0.842
0.825SerCys: 0.825 ± 0.247
4.698SerAsp: 4.698 ± 0.523
4.19SerGlu: 4.19 ± 0.458
1.904SerPhe: 1.904 ± 0.325
6.094SerGly: 6.094 ± 0.624
0.635SerHis: 0.635 ± 0.221
4.253SerIle: 4.253 ± 0.583
3.237SerLys: 3.237 ± 0.47
6.665SerLeu: 6.665 ± 0.668
2.412SerMet: 2.412 ± 0.442
2.095SerAsn: 2.095 ± 0.301
2.412SerPro: 2.412 ± 0.463
2.285SerGln: 2.285 ± 0.35
3.682SerArg: 3.682 ± 0.485
4.317SerSer: 4.317 ± 0.645
3.301SerThr: 3.301 ± 0.696
4.38SerVal: 4.38 ± 0.53
0.698SerTrp: 0.698 ± 0.221
2.412SerTyr: 2.412 ± 0.41
0.0SerXaa: 0.0 ± 0.0
Thr
7.11ThrAla: 7.11 ± 0.965
0.254ThrCys: 0.254 ± 0.129
3.618ThrAsp: 3.618 ± 0.569
3.999ThrGlu: 3.999 ± 0.513
2.412ThrPhe: 2.412 ± 0.429
5.967ThrGly: 5.967 ± 0.767
0.889ThrHis: 0.889 ± 0.218
3.745ThrIle: 3.745 ± 0.524
3.047ThrLys: 3.047 ± 0.466
4.317ThrLeu: 4.317 ± 0.514
1.27ThrMet: 1.27 ± 0.291
1.904ThrAsn: 1.904 ± 0.267
2.857ThrPro: 2.857 ± 0.498
2.412ThrGln: 2.412 ± 0.541
2.793ThrArg: 2.793 ± 0.382
3.809ThrSer: 3.809 ± 0.581
3.428ThrThr: 3.428 ± 0.628
4.38ThrVal: 4.38 ± 0.446
0.889ThrTrp: 0.889 ± 0.243
1.904ThrTyr: 1.904 ± 0.42
0.0ThrXaa: 0.0 ± 0.0
Val
6.348ValAla: 6.348 ± 0.607
0.825ValCys: 0.825 ± 0.225
3.745ValAsp: 3.745 ± 0.68
3.999ValGlu: 3.999 ± 0.505
2.349ValPhe: 2.349 ± 0.407
3.555ValGly: 3.555 ± 0.41
0.444ValHis: 0.444 ± 0.208
4.634ValIle: 4.634 ± 0.597
3.555ValLys: 3.555 ± 0.509
4.761ValLeu: 4.761 ± 0.501
1.27ValMet: 1.27 ± 0.247
3.555ValAsn: 3.555 ± 0.53
2.349ValPro: 2.349 ± 0.392
1.777ValGln: 1.777 ± 0.378
3.618ValArg: 3.618 ± 0.47
5.015ValSer: 5.015 ± 0.596
4.951ValThr: 4.951 ± 0.593
3.745ValVal: 3.745 ± 0.433
0.698ValTrp: 0.698 ± 0.22
1.524ValTyr: 1.524 ± 0.298
0.0ValXaa: 0.0 ± 0.0
Trp
1.333TrpAla: 1.333 ± 0.319
0.19TrpCys: 0.19 ± 0.105
0.571TrpAsp: 0.571 ± 0.17
0.698TrpGlu: 0.698 ± 0.242
0.254TrpPhe: 0.254 ± 0.164
0.381TrpGly: 0.381 ± 0.151
0.127TrpHis: 0.127 ± 0.103
0.571TrpIle: 0.571 ± 0.209
1.46TrpLys: 1.46 ± 0.287
1.524TrpLeu: 1.524 ± 0.389
0.254TrpMet: 0.254 ± 0.125
0.698TrpAsn: 0.698 ± 0.218
0.698TrpPro: 0.698 ± 0.223
0.762TrpGln: 0.762 ± 0.186
1.143TrpArg: 1.143 ± 0.259
1.079TrpSer: 1.079 ± 0.272
1.016TrpThr: 1.016 ± 0.32
1.079TrpVal: 1.079 ± 0.296
0.444TrpTrp: 0.444 ± 0.207
0.317TrpTyr: 0.317 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.412TyrAla: 2.412 ± 0.511
0.381TyrCys: 0.381 ± 0.146
1.777TyrAsp: 1.777 ± 0.355
2.158TyrGlu: 2.158 ± 0.259
0.825TyrPhe: 0.825 ± 0.231
2.539TyrGly: 2.539 ± 0.386
0.698TyrHis: 0.698 ± 0.192
1.333TyrIle: 1.333 ± 0.268
1.777TyrLys: 1.777 ± 0.361
2.793TyrLeu: 2.793 ± 0.515
0.952TyrMet: 0.952 ± 0.232
1.079TyrAsn: 1.079 ± 0.281
1.206TyrPro: 1.206 ± 0.284
1.143TyrGln: 1.143 ± 0.275
2.412TyrArg: 2.412 ± 0.346
2.412TyrSer: 2.412 ± 0.437
2.349TyrThr: 2.349 ± 0.414
2.476TyrVal: 2.476 ± 0.391
0.254TyrTrp: 0.254 ± 0.118
0.762TyrTyr: 0.762 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (15754 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski