Bacillus virus Riggi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5PW67|U5PW67_9CAUD Uncharacterized protein OS=Bacillus virus Riggi OX=1406788 GN=Riggi_7 PE=4 SV=1
MM1 pKa = 6.93MVRR4 pKa = 11.84MFANYY9 pKa = 10.05EE10 pKa = 4.05NIEE13 pKa = 4.18MNNDD17 pKa = 3.01VLIVSNKK24 pKa = 9.42KK25 pKa = 10.59DD26 pKa = 3.55YY27 pKa = 11.2FEE29 pKa = 5.96PNTFSSYY36 pKa = 9.32PVPVIVDD43 pKa = 3.52HH44 pKa = 7.01EE45 pKa = 4.38CLQFHH50 pKa = 7.15HH51 pKa = 7.09YY52 pKa = 10.48DD53 pKa = 3.66KK54 pKa = 11.34QDD56 pKa = 3.19DD57 pKa = 3.97TMLYY61 pKa = 7.99TVCSEE66 pKa = 5.25DD67 pKa = 3.38IDD69 pKa = 4.75SIYY72 pKa = 11.36

Molecular weight: 8.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5PZV8|U5PZV8_9CAUD Uncharacterized protein OS=Bacillus virus Riggi OX=1406788 GN=Riggi_25 PE=4 SV=1
MM1 pKa = 7.48SNVNMMISVDD11 pKa = 3.85LNNVPEE17 pKa = 4.64HH18 pKa = 6.57LAQAAKK24 pKa = 10.06KK25 pKa = 10.28VSDD28 pKa = 3.76FKK30 pKa = 11.72VPLRR34 pKa = 11.84QSAVYY39 pKa = 9.16MEE41 pKa = 4.55GSISRR46 pKa = 11.84RR47 pKa = 11.84FSSGGGSQGKK57 pKa = 7.77WKK59 pKa = 10.04RR60 pKa = 11.84LSPATIRR67 pKa = 11.84RR68 pKa = 11.84HH69 pKa = 4.26PHH71 pKa = 5.88RR72 pKa = 11.84SGGTPLSDD80 pKa = 3.1TGRR83 pKa = 11.84LKK85 pKa = 10.92QSVTSGAVKK94 pKa = 10.14QYY96 pKa = 10.82SPKK99 pKa = 9.68QLRR102 pKa = 11.84YY103 pKa = 7.94TLGANVKK110 pKa = 8.54YY111 pKa = 10.9AKK113 pKa = 9.79VHH115 pKa = 5.39NFGGGRR121 pKa = 11.84IPARR125 pKa = 11.84PFMYY129 pKa = 10.04TDD131 pKa = 2.97AKK133 pKa = 10.86DD134 pKa = 3.36RR135 pKa = 11.84QEE137 pKa = 3.84VVKK140 pKa = 10.76IFTEE144 pKa = 4.43FLRR147 pKa = 11.84GVANQQ152 pKa = 3.21

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

15050

30

1026

192.9

21.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.867 ± 0.378

0.963 ± 0.137

6.173 ± 0.29

7.894 ± 0.533

4.571 ± 0.187

6.671 ± 0.434

2.179 ± 0.181

6.678 ± 0.202

8.312 ± 0.499

7.967 ± 0.339

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.184

5.043 ± 0.277

3.316 ± 0.164

3.648 ± 0.177

4.452 ± 0.212

6.259 ± 0.37

5.973 ± 0.395

6.14 ± 0.241

1.136 ± 0.115

3.847 ± 0.251
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski