Tortoise microvirus 1

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W915|A0A4P8W915_9VIRU Replication initiation protein OS=Tortoise microvirus 1 OX=2583098 PE=4 SV=1
MM1 pKa = 7.0SQNIFDD7 pKa = 4.12ATFDD11 pKa = 3.85ANNQINVNSFDD22 pKa = 3.46WSHH25 pKa = 5.82VNNLTTSFGRR35 pKa = 11.84VTPVFCEE42 pKa = 3.92LVPAKK47 pKa = 10.42GSLRR51 pKa = 11.84INPEE55 pKa = 3.61FGLEE59 pKa = 3.97LMPMVFPVQTRR70 pKa = 11.84MYY72 pKa = 11.01ARR74 pKa = 11.84LNFFKK79 pKa = 10.48VTLRR83 pKa = 11.84SMWEE87 pKa = 4.14DD88 pKa = 3.36YY89 pKa = 11.61SDD91 pKa = 4.78FISNFRR97 pKa = 11.84DD98 pKa = 3.77DD99 pKa = 5.34LEE101 pKa = 4.37EE102 pKa = 4.61PYY104 pKa = 10.41ILPDD108 pKa = 3.71FVRR111 pKa = 11.84FAKK114 pKa = 9.32MCKK117 pKa = 9.11TGTLGDD123 pKa = 4.0YY124 pKa = 10.89LGLPTFGTNISQSVTIPDD142 pKa = 3.68SPCTVNSRR150 pKa = 11.84NFTTSVEE157 pKa = 4.22VNAAMASIRR166 pKa = 11.84QDD168 pKa = 3.09ATPAGLICANHH179 pKa = 6.37FNTDD183 pKa = 2.84GKK185 pKa = 10.11GTVIFKK191 pKa = 6.96TQRR194 pKa = 11.84SATQIPQSVSSVGINIGIVGSSADD218 pKa = 3.44AFVGCRR224 pKa = 11.84GYY226 pKa = 10.76LVHH229 pKa = 7.22YY230 pKa = 9.92GDD232 pKa = 5.52DD233 pKa = 3.35GTYY236 pKa = 9.62YY237 pKa = 10.5SDD239 pKa = 4.14YY240 pKa = 9.15PVEE243 pKa = 4.39VIKK246 pKa = 11.13SDD248 pKa = 3.73DD249 pKa = 3.66GVVGVVISSIDD260 pKa = 3.05IGSFSNALGGLDD272 pKa = 4.27LVLNAPNSANPIEE285 pKa = 4.37FTGMQYY291 pKa = 11.14NVVVSYY297 pKa = 8.34YY298 pKa = 9.66TLQSDD303 pKa = 3.99VSYY306 pKa = 10.43TKK308 pKa = 11.02YY309 pKa = 10.34PFATEE314 pKa = 3.53EE315 pKa = 4.06HH316 pKa = 6.82LNFPRR321 pKa = 11.84LLAYY325 pKa = 10.09RR326 pKa = 11.84FRR328 pKa = 11.84AYY330 pKa = 10.2EE331 pKa = 4.04SVYY334 pKa = 9.54NAYY337 pKa = 10.51YY338 pKa = 10.44RR339 pKa = 11.84DD340 pKa = 3.2IRR342 pKa = 11.84NNPFVINGRR351 pKa = 11.84PVYY354 pKa = 10.54NKK356 pKa = 9.03WLPNMKK362 pKa = 10.16GGADD366 pKa = 3.25TTLYY370 pKa = 10.46EE371 pKa = 3.95LHH373 pKa = 5.6QCNWEE378 pKa = 3.97KK379 pKa = 11.33DD380 pKa = 3.84FLTTAVPNPQQGTNPPLVGLTVGDD404 pKa = 3.96VVTRR408 pKa = 11.84TSDD411 pKa = 2.99GTLQVQKK418 pKa = 9.47QTVLVDD424 pKa = 3.41EE425 pKa = 5.81DD426 pKa = 3.95GAKK429 pKa = 10.61YY430 pKa = 10.13GISYY434 pKa = 10.01KK435 pKa = 10.42VSEE438 pKa = 5.18DD439 pKa = 3.46GQSLVGVDD447 pKa = 3.92YY448 pKa = 11.39DD449 pKa = 3.9PVSDD453 pKa = 4.28KK454 pKa = 11.43TPITQINSYY463 pKa = 11.33AEE465 pKa = 4.17LASLATEE472 pKa = 3.97QGSGFTIEE480 pKa = 3.8TLRR483 pKa = 11.84YY484 pKa = 8.15VNAYY488 pKa = 10.02QKK490 pKa = 10.64FLEE493 pKa = 4.19LNMRR497 pKa = 11.84KK498 pKa = 9.68GFSYY502 pKa = 9.93KK503 pKa = 10.37QIMQGRR509 pKa = 11.84WDD511 pKa = 3.19INIRR515 pKa = 11.84FDD517 pKa = 3.83EE518 pKa = 4.39LLMPEE523 pKa = 4.69FIGGVSRR530 pKa = 11.84EE531 pKa = 3.73LSMRR535 pKa = 11.84TVEE538 pKa = 4.11QTVDD542 pKa = 3.26QQTEE546 pKa = 3.88ISQGQYY552 pKa = 11.21AEE554 pKa = 4.34ALGSKK559 pKa = 8.88TGIAGVYY566 pKa = 10.3GYY568 pKa = 11.41GNGNIEE574 pKa = 4.2VFCDD578 pKa = 3.47EE579 pKa = 4.08EE580 pKa = 5.21SIIIGLLTVTPVPVYY595 pKa = 9.03TQLMPKK601 pKa = 10.27DD602 pKa = 3.52FLYY605 pKa = 11.18NGLLDD610 pKa = 4.32HH611 pKa = 6.36YY612 pKa = 10.57QPEE615 pKa = 4.21FDD617 pKa = 5.15RR618 pKa = 11.84IGFQPITYY626 pKa = 10.11KK627 pKa = 10.3EE628 pKa = 4.25VCPMNIPAYY637 pKa = 10.41DD638 pKa = 3.61DD639 pKa = 3.78TNQLEE644 pKa = 4.38QTFGYY649 pKa = 8.8QRR651 pKa = 11.84PWYY654 pKa = 9.05EE655 pKa = 3.53YY656 pKa = 8.47VAKK659 pKa = 10.41YY660 pKa = 10.67DD661 pKa = 3.64SAHH664 pKa = 5.62GLFRR668 pKa = 11.84TSMKK672 pKa = 10.31NFIMSRR678 pKa = 11.84VFRR681 pKa = 11.84GLPQLGQEE689 pKa = 4.52FLLVDD694 pKa = 4.51PNTTNQVFAVTEE706 pKa = 4.01YY707 pKa = 10.13SDD709 pKa = 4.98KK710 pKa = 10.98IFGYY714 pKa = 10.82VKK716 pKa = 10.25FNATARR722 pKa = 11.84LPISRR727 pKa = 11.84VAIPRR732 pKa = 11.84LDD734 pKa = 3.18

Molecular weight: 82.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V1FVV8|A0A4V1FVV8_9VIRU Uncharacterized protein OS=Tortoise microvirus 1 OX=2583098 PE=4 SV=1
MM1 pKa = 7.73LLQLWCEE8 pKa = 3.85NNFFFFLPTLHH19 pKa = 6.98SLAFCVSCSKK29 pKa = 10.78GYY31 pKa = 9.12SVKK34 pKa = 10.16FCYY37 pKa = 10.4NFGACGKK44 pKa = 9.98ILIKK48 pKa = 10.54FNGMFIGLLTQNILSLQFRR67 pKa = 11.84KK68 pKa = 10.04IKK70 pKa = 10.29GKK72 pKa = 10.62SIFPLRR78 pKa = 11.84EE79 pKa = 3.53TQINSNFVACVRR91 pKa = 11.84DD92 pKa = 3.82CRR94 pKa = 11.84RR95 pKa = 11.84VSS97 pKa = 3.02

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1842

75

734

307.0

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.003 ± 1.528

1.9 ± 0.749

5.049 ± 0.587

4.832 ± 0.401

5.646 ± 0.917

6.026 ± 0.861

1.466 ± 0.678

5.809 ± 0.369

5.809 ± 1.096

7.329 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.312

6.515 ± 0.347

4.669 ± 0.478

4.94 ± 0.952

4.669 ± 0.21

7.275 ± 0.328

5.537 ± 0.858

6.406 ± 0.95

0.869 ± 0.127

5.483 ± 1.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski