Tetrasphaera phage TJE1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tijeunavirus; Tetrasphaera virus TJE1

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4W975|G4W975_9CAUD Uncharacterized protein OS=Tetrasphaera phage TJE1 OX=981335 PE=4 SV=1
MM1 pKa = 7.78NDD3 pKa = 3.14QDD5 pKa = 5.18LASQFDD11 pKa = 4.03GPYY14 pKa = 10.16SQGLMTAIQAEE25 pKa = 4.07IDD27 pKa = 3.55VRR29 pKa = 11.84AVIIQYY35 pKa = 10.57LEE37 pKa = 3.98SLSIDD42 pKa = 3.41SAGPTEE48 pKa = 4.65LSTIGGIIGYY58 pKa = 7.05PWPAAPTGTFDD69 pKa = 3.63GNAFVFGSSDD79 pKa = 3.91LFPSISDD86 pKa = 3.07IYY88 pKa = 10.12GFSGASLPGTGGIFTSSTPEE108 pKa = 3.72AGNIIPISFYY118 pKa = 11.0RR119 pKa = 11.84LLLSQVAYY127 pKa = 10.78LKK129 pKa = 10.25ATGLSYY135 pKa = 10.12TAIDD139 pKa = 3.89KK140 pKa = 10.24ICYY143 pKa = 9.09VFSPDD148 pKa = 3.71YY149 pKa = 10.81MFFNPLLEE157 pKa = 5.17DD158 pKa = 4.39NIFQLGAAEE167 pKa = 4.77DD168 pKa = 4.54FPNSSSTHH176 pKa = 5.34GLSGVDD182 pKa = 4.89IIYY185 pKa = 10.18HH186 pKa = 5.55DD187 pKa = 3.69QGGVLTTAVSGGVVDD202 pKa = 3.7SDD204 pKa = 3.67IYY206 pKa = 10.8IIFNTPIGSGYY217 pKa = 10.83LWILQALFDD226 pKa = 4.69RR227 pKa = 11.84FTTAPKK233 pKa = 9.89IYY235 pKa = 9.97VIQGGLL241 pKa = 3.18

Molecular weight: 25.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4W956|G4W956_9CAUD Uncharacterized protein OS=Tetrasphaera phage TJE1 OX=981335 PE=4 SV=1
MM1 pKa = 7.47EE2 pKa = 4.69LQMRR6 pKa = 11.84VPRR9 pKa = 11.84CVRR12 pKa = 11.84THH14 pKa = 6.54LPDD17 pKa = 3.49GRR19 pKa = 11.84HH20 pKa = 5.77PGRR23 pKa = 11.84RR24 pKa = 11.84HH25 pKa = 5.02KK26 pKa = 10.32AGRR29 pKa = 11.84MIDD32 pKa = 3.76SAKK35 pKa = 10.26LPPVGSLLRR44 pKa = 11.84GARR47 pKa = 11.84LRR49 pKa = 11.84MDD51 pKa = 4.37LTLKK55 pKa = 10.55DD56 pKa = 3.53ASCRR60 pKa = 11.84IGIHH64 pKa = 5.45MLLLRR69 pKa = 11.84QYY71 pKa = 10.11EE72 pKa = 4.14VGEE75 pKa = 4.5RR76 pKa = 11.84YY77 pKa = 9.31PSHH80 pKa = 6.3KK81 pKa = 10.13AVRR84 pKa = 11.84RR85 pKa = 11.84LAMFYY90 pKa = 10.98GLTLKK95 pKa = 10.35EE96 pKa = 4.04VYY98 pKa = 10.03EE99 pKa = 4.47RR100 pKa = 11.84FAQEE104 pKa = 4.35DD105 pKa = 4.11FTNEE109 pKa = 3.74RR110 pKa = 11.84SVSAVPATATKK121 pKa = 10.7PEE123 pKa = 3.96MGAA126 pKa = 3.38

Molecular weight: 14.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

14123

61

728

214.0

23.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.063 ± 0.46

0.843 ± 0.086

5.572 ± 0.226

6.387 ± 0.51

4.192 ± 0.175

8.051 ± 0.282

1.721 ± 0.166

5.133 ± 0.21

5.594 ± 0.357

8.369 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.154

3.781 ± 0.211

5.155 ± 0.312

4.241 ± 0.241

5.367 ± 0.329

6.167 ± 0.301

6.139 ± 0.296

6.833 ± 0.247

1.841 ± 0.126

3.059 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski