Neorhizobium alkalisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Neorhizobium

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6627 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A561R7N3|A0A561R7N3_9RHIZ Cytochrome bo(3) ubiquinol oxidase subunit 4 OS=Neorhizobium alkalisoli OX=528178 GN=FHW37_101431 PE=3 SV=1
MM1 pKa = 7.46TKK3 pKa = 9.46TVLNAVGDD11 pKa = 3.87TLYY14 pKa = 11.33YY15 pKa = 10.88SGDD18 pKa = 3.35PTAWFSATGSGPTLAGSAGNDD39 pKa = 2.78AMYY42 pKa = 10.95GNSAVNVTMSGGTGDD57 pKa = 3.84DD58 pKa = 3.13VYY60 pKa = 11.81YY61 pKa = 10.47LYY63 pKa = 11.24SSINRR68 pKa = 11.84AVEE71 pKa = 4.11SADD74 pKa = 4.0GGIDD78 pKa = 4.31TINTWMSYY86 pKa = 7.36TLPDD90 pKa = 4.12NIEE93 pKa = 4.01NLTVTGAGRR102 pKa = 11.84YY103 pKa = 9.31AFGNSLDD110 pKa = 4.31NIISGAAGTQTIDD123 pKa = 2.92GGAGNDD129 pKa = 3.45VLTGGGGADD138 pKa = 2.95IFIVTKK144 pKa = 10.96GNGSDD149 pKa = 4.77LITDD153 pKa = 4.42FGADD157 pKa = 3.26DD158 pKa = 4.68TIRR161 pKa = 11.84LNKK164 pKa = 10.38YY165 pKa = 9.25GFTSFEE171 pKa = 3.97QLSSHH176 pKa = 6.29FTQEE180 pKa = 3.92GANLRR185 pKa = 11.84LDD187 pKa = 4.09LGDD190 pKa = 4.53GEE192 pKa = 5.12SLLLANTSADD202 pKa = 3.48QLSEE206 pKa = 4.31SQFQLPLDD214 pKa = 4.2RR215 pKa = 11.84SHH217 pKa = 7.01LTQTFGDD224 pKa = 3.91EE225 pKa = 4.37FNNLSLFNGTSGTWDD240 pKa = 3.62AKK242 pKa = 10.74FPWAPTEE249 pKa = 4.35GSSLPTNGEE258 pKa = 3.96LQWYY262 pKa = 7.55VNPAYY267 pKa = 10.41GPTASANPFSVSNGVLTITAKK288 pKa = 9.54DD289 pKa = 3.45TPDD292 pKa = 3.42ALKK295 pKa = 9.7DD296 pKa = 3.58TVGGYY301 pKa = 10.59DD302 pKa = 3.55YY303 pKa = 11.29TSGLLTTASTFSQTYY318 pKa = 8.94GYY320 pKa = 10.75FEE322 pKa = 4.66IRR324 pKa = 11.84ADD326 pKa = 3.7MPTDD330 pKa = 3.29TGVWPAFWLLPEE342 pKa = 5.2DD343 pKa = 4.82GSWPPEE349 pKa = 3.78LDD351 pKa = 3.52VVEE354 pKa = 4.8MRR356 pKa = 11.84GQQPNTVHH364 pKa = 6.66VSAHH368 pKa = 5.94SNATGQQTTQTSAVQVPSTDD388 pKa = 3.05GFHH391 pKa = 6.83TYY393 pKa = 9.84GVLWGEE399 pKa = 5.0DD400 pKa = 3.49EE401 pKa = 4.36IVWYY405 pKa = 10.31FDD407 pKa = 3.68DD408 pKa = 4.53VAIAHH413 pKa = 7.29ADD415 pKa = 3.77TPDD418 pKa = 4.05DD419 pKa = 3.6MHH421 pKa = 8.45KK422 pKa = 10.49PMYY425 pKa = 9.59MLVDD429 pKa = 4.11LAVGGVAGTPPNGLPNGSKK448 pKa = 8.65MQIDD452 pKa = 4.52YY453 pKa = 10.83IHH455 pKa = 7.4AYY457 pKa = 10.4SLDD460 pKa = 4.09DD461 pKa = 3.97PSVATMVSSSAQDD474 pKa = 3.45GLVV477 pKa = 2.94

Molecular weight: 50.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A561Q8J0|A0A561Q8J0_9RHIZ N-methylhydantoinase A/oxoprolinase/acetone carboxylase beta subunit OS=Neorhizobium alkalisoli OX=528178 GN=FHW37_113115 PE=4 SV=1
MM1 pKa = 7.08KK2 pKa = 10.01HH3 pKa = 5.63IVMRR7 pKa = 11.84AKK9 pKa = 10.43INWKK13 pKa = 7.64LHH15 pKa = 4.63RR16 pKa = 11.84RR17 pKa = 11.84ANVIRR22 pKa = 11.84AFAGAPAKK30 pKa = 10.28SHH32 pKa = 6.22VSIAPP37 pKa = 3.3

Molecular weight: 4.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6627

0

6627

2057094

26

8326

310.4

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.786 ± 0.039

0.765 ± 0.009

5.792 ± 0.033

5.699 ± 0.036

3.942 ± 0.02

8.407 ± 0.035

1.964 ± 0.015

5.791 ± 0.024

3.874 ± 0.033

9.847 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.018

2.886 ± 0.027

4.858 ± 0.023

3.119 ± 0.021

6.375 ± 0.038

5.973 ± 0.024

5.438 ± 0.053

7.275 ± 0.023

1.251 ± 0.014

2.319 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski