Amino acid dipepetide frequency for Rhodobacteraceae bacterium W635

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.021AlaAla: 18.021 ± 0.202	1.18AlaCys: 1.18 ± 0.037	7.331AlaAsp: 7.331 ± 0.096	9.163AlaGlu: 9.163 ± 0.115	4.307AlaPhe: 4.307 ± 0.073	11.725AlaGly: 11.725 ± 0.115	2.644AlaHis: 2.644 ± 0.053	5.836AlaIle: 5.836 ± 0.092	3.095AlaLys: 3.095 ± 0.072	14.234AlaLeu: 14.234 ± 0.159	4.013AlaMet: 4.013 ± 0.067	2.477AlaAsn: 2.477 ± 0.05	6.553AlaPro: 6.553 ± 0.115	4.215AlaGln: 4.215 ± 0.065	10.545AlaArg: 10.545 ± 0.126	5.147AlaSer: 5.147 ± 0.07	6.327AlaThr: 6.327 ± 0.082	8.524AlaVal: 8.524 ± 0.108	1.44AlaTrp: 1.44 ± 0.038	2.539AlaTyr: 2.539 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.088CysAla: 1.088 ± 0.036	0.115CysCys: 0.115 ± 0.011	0.661CysAsp: 0.661 ± 0.025	0.441CysGlu: 0.441 ± 0.019	0.32CysPhe: 0.32 ± 0.018	0.965CysGly: 0.965 ± 0.032	0.253CysHis: 0.253 ± 0.017	0.396CysIle: 0.396 ± 0.02	0.17CysLys: 0.17 ± 0.013	0.908CysLeu: 0.908 ± 0.027	0.161CysMet: 0.161 ± 0.012	0.231CysAsn: 0.231 ± 0.016	0.581CysPro: 0.581 ± 0.026	0.207CysGln: 0.207 ± 0.014	0.605CysArg: 0.605 ± 0.028	0.44CysSer: 0.44 ± 0.022	0.465CysThr: 0.465 ± 0.022	0.568CysVal: 0.568 ± 0.024	0.131CysTrp: 0.131 ± 0.011	0.206CysTyr: 0.206 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.697AspAla: 7.697 ± 0.091	0.593AspCys: 0.593 ± 0.026	3.557AspAsp: 3.557 ± 0.078	3.375AspGlu: 3.375 ± 0.065	2.344AspPhe: 2.344 ± 0.052	5.886AspGly: 5.886 ± 0.087	1.627AspHis: 1.627 ± 0.045	3.08AspIle: 3.08 ± 0.051	1.411AspLys: 1.411 ± 0.043	6.967AspLeu: 6.967 ± 0.108	1.796AspMet: 1.796 ± 0.046	1.181AspAsn: 1.181 ± 0.037	4.332AspPro: 4.332 ± 0.07	1.682AspGln: 1.682 ± 0.044	5.281AspArg: 5.281 ± 0.076	2.414AspSer: 2.414 ± 0.048	3.172AspThr: 3.172 ± 0.071	4.205AspVal: 4.205 ± 0.056	1.409AspTrp: 1.409 ± 0.042	1.54AspTyr: 1.54 ± 0.038	0.0AspXaa: 0.0 ± 0.0
Glu	9.154GluAla: 9.154 ± 0.111	0.328GluCys: 0.328 ± 0.018	3.686GluAsp: 3.686 ± 0.063	3.766GluGlu: 3.766 ± 0.076	1.617GluPhe: 1.617 ± 0.041	4.976GluGly: 4.976 ± 0.07	1.032GluHis: 1.032 ± 0.035	3.572GluIle: 3.572 ± 0.061	1.754GluLys: 1.754 ± 0.053	4.666GluLeu: 4.666 ± 0.074	1.899GluMet: 1.899 ± 0.042	1.526GluAsn: 1.526 ± 0.044	2.406GluPro: 2.406 ± 0.051	1.724GluGln: 1.724 ± 0.045	4.185GluArg: 4.185 ± 0.059	2.276GluSer: 2.276 ± 0.051	4.157GluThr: 4.157 ± 0.073	4.598GluVal: 4.598 ± 0.068	0.68GluTrp: 0.68 ± 0.031	0.922GluTyr: 0.922 ± 0.032	0.0GluXaa: 0.0 ± 0.0
Phe	4.441PheAla: 4.441 ± 0.073	0.434PheCys: 0.434 ± 0.02	2.756PheAsp: 2.756 ± 0.055	2.22PheGlu: 2.22 ± 0.049	1.297PhePhe: 1.297 ± 0.043	3.602PheGly: 3.602 ± 0.065	0.786PheHis: 0.786 ± 0.028	1.556PheIle: 1.556 ± 0.045	0.713PheLys: 0.713 ± 0.026	3.489PheLeu: 3.489 ± 0.066	0.832PheMet: 0.832 ± 0.028	0.963PheAsn: 0.963 ± 0.03	1.533PhePro: 1.533 ± 0.039	0.918PheGln: 0.918 ± 0.027	2.266PheArg: 2.266 ± 0.045	1.822PheSer: 1.822 ± 0.044	2.109PheThr: 2.109 ± 0.045	2.497PheVal: 2.497 ± 0.047	0.602PheTrp: 0.602 ± 0.028	0.868PheTyr: 0.868 ± 0.034	0.0PheXaa: 0.0 ± 0.0
Gly	10.86GlyAla: 10.86 ± 0.139	0.92GlyCys: 0.92 ± 0.033	5.193GlyAsp: 5.193 ± 0.102	4.89GlyGlu: 4.89 ± 0.077	3.719GlyPhe: 3.719 ± 0.062	7.95GlyGly: 7.95 ± 0.128	2.12GlyHis: 2.12 ± 0.044	4.389GlyIle: 4.389 ± 0.073	2.696GlyLys: 2.696 ± 0.059	9.65GlyLeu: 9.65 ± 0.115	2.764GlyMet: 2.764 ± 0.051	1.995GlyAsn: 1.995 ± 0.051	4.127GlyPro: 4.127 ± 0.066	3.296GlyGln: 3.296 ± 0.053	6.495GlyArg: 6.495 ± 0.091	3.979GlySer: 3.979 ± 0.067	4.704GlyThr: 4.704 ± 0.071	6.55GlyVal: 6.55 ± 0.084	1.651GlyTrp: 1.651 ± 0.044	2.326GlyTyr: 2.326 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.632HisAla: 2.632 ± 0.054	0.241HisCys: 0.241 ± 0.014	1.533HisAsp: 1.533 ± 0.043	1.144HisGlu: 1.144 ± 0.031	0.825HisPhe: 0.825 ± 0.027	2.206HisGly: 2.206 ± 0.051	0.619HisHis: 0.619 ± 0.03	0.895HisIle: 0.895 ± 0.029	0.463HisLys: 0.463 ± 0.021	2.242HisLeu: 2.242 ± 0.054	0.552HisMet: 0.552 ± 0.024	0.432HisAsn: 0.432 ± 0.024	1.474HisPro: 1.474 ± 0.037	0.506HisGln: 0.506 ± 0.021	1.47HisArg: 1.47 ± 0.044	0.871HisSer: 0.871 ± 0.031	0.799HisThr: 0.799 ± 0.027	1.754HisVal: 1.754 ± 0.042	0.382HisTrp: 0.382 ± 0.021	0.602HisTyr: 0.602 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.984IleAla: 6.984 ± 0.088	0.557IleCys: 0.557 ± 0.02	3.29IleAsp: 3.29 ± 0.066	3.159IleGlu: 3.159 ± 0.058	1.626IlePhe: 1.626 ± 0.04	4.916IleGly: 4.916 ± 0.076	0.947IleHis: 0.947 ± 0.032	1.924IleIle: 1.924 ± 0.048	1.035IleLys: 1.035 ± 0.032	4.681IleLeu: 4.681 ± 0.071	1.003IleMet: 1.003 ± 0.037	1.214IleAsn: 1.214 ± 0.039	2.325IlePro: 2.325 ± 0.053	0.921IleGln: 0.921 ± 0.032	3.351IleArg: 3.351 ± 0.056	2.436IleSer: 2.436 ± 0.052	2.84IleThr: 2.84 ± 0.048	3.596IleVal: 3.596 ± 0.062	0.67IleTrp: 0.67 ± 0.025	1.037IleTyr: 1.037 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	3.406LysAla: 3.406 ± 0.067	0.149LysCys: 0.149 ± 0.012	1.385LysAsp: 1.385 ± 0.037	1.204LysGlu: 1.204 ± 0.04	0.649LysPhe: 0.649 ± 0.024	2.305LysGly: 2.305 ± 0.05	0.519LysHis: 0.519 ± 0.024	1.21LysIle: 1.21 ± 0.038	0.888LysLys: 0.888 ± 0.04	2.437LysLeu: 2.437 ± 0.057	0.682LysMet: 0.682 ± 0.028	0.522LysAsn: 0.522 ± 0.028	1.536LysPro: 1.536 ± 0.043	0.697LysGln: 0.697 ± 0.024	1.903LysArg: 1.903 ± 0.045	1.399LysSer: 1.399 ± 0.048	1.627LysThr: 1.627 ± 0.045	1.91LysVal: 1.91 ± 0.057	0.301LysTrp: 0.301 ± 0.017	0.453LysTyr: 0.453 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	13.619LeuAla: 13.619 ± 0.147	0.951LeuCys: 0.951 ± 0.032	6.851LeuAsp: 6.851 ± 0.101	5.077LeuGlu: 5.077 ± 0.08	3.556LeuPhe: 3.556 ± 0.082	8.415LeuGly: 8.415 ± 0.096	2.038LeuHis: 2.038 ± 0.048	4.862LeuIle: 4.862 ± 0.082	2.652LeuLys: 2.652 ± 0.054	9.096LeuLeu: 9.096 ± 0.153	2.654LeuMet: 2.654 ± 0.052	2.376LeuAsn: 2.376 ± 0.054	5.854LeuPro: 5.854 ± 0.082	2.736LeuGln: 2.736 ± 0.056	7.697LeuArg: 7.697 ± 0.082	6.538LeuSer: 6.538 ± 0.086	5.953LeuThr: 5.953 ± 0.084	7.197LeuVal: 7.197 ± 0.094	1.258LeuTrp: 1.258 ± 0.037	1.958LeuTyr: 1.958 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	3.788MetAla: 3.788 ± 0.068	0.177MetCys: 0.177 ± 0.012	1.559MetAsp: 1.559 ± 0.041	1.388MetGlu: 1.388 ± 0.038	0.732MetPhe: 0.732 ± 0.028	2.356MetGly: 2.356 ± 0.055	0.495MetHis: 0.495 ± 0.024	1.476MetIle: 1.476 ± 0.034	0.859MetLys: 0.859 ± 0.026	2.679MetLeu: 2.679 ± 0.055	0.744MetMet: 0.744 ± 0.027	0.759MetAsn: 0.759 ± 0.027	1.535MetPro: 1.535 ± 0.038	1.04MetGln: 1.04 ± 0.028	2.081MetArg: 2.081 ± 0.044	1.624MetSer: 1.624 ± 0.042	2.18MetThr: 2.18 ± 0.045	1.926MetVal: 1.926 ± 0.045	0.25MetTrp: 0.25 ± 0.018	0.322MetTyr: 0.322 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.02AsnAla: 3.02 ± 0.055	0.226AsnCys: 0.226 ± 0.016	1.339AsnAsp: 1.339 ± 0.041	1.043AsnGlu: 1.043 ± 0.033	0.851AsnPhe: 0.851 ± 0.033	2.062AsnGly: 2.062 ± 0.051	0.498AsnHis: 0.498 ± 0.023	1.179AsnIle: 1.179 ± 0.038	0.48AsnLys: 0.48 ± 0.024	2.253AsnLeu: 2.253 ± 0.047	0.655AsnMet: 0.655 ± 0.026	0.558AsnAsn: 0.558 ± 0.024	1.698AsnPro: 1.698 ± 0.041	0.599AsnGln: 0.599 ± 0.024	1.673AsnArg: 1.673 ± 0.043	0.94AsnSer: 0.94 ± 0.034	1.195AsnThr: 1.195 ± 0.03	1.67AsnVal: 1.67 ± 0.036	0.411AsnTrp: 0.411 ± 0.019	0.575AsnTyr: 0.575 ± 0.026	0.0AsnXaa: 0.0 ± 0.0
Pro	6.304ProAla: 6.304 ± 0.1	0.406ProCys: 0.406 ± 0.021	4.631ProAsp: 4.631 ± 0.076	4.329ProGlu: 4.329 ± 0.064	1.925ProPhe: 1.925 ± 0.045	5.432ProGly: 5.432 ± 0.076	1.111ProHis: 1.111 ± 0.039	2.078ProIle: 2.078 ± 0.043	1.282ProLys: 1.282 ± 0.034	4.757ProLeu: 4.757 ± 0.08	1.393ProMet: 1.393 ± 0.034	1.18ProAsn: 1.18 ± 0.036	2.476ProPro: 2.476 ± 0.061	1.566ProGln: 1.566 ± 0.044	3.338ProArg: 3.338 ± 0.06	2.257ProSer: 2.257 ± 0.049	2.415ProThr: 2.415 ± 0.045	4.657ProVal: 4.657 ± 0.061	0.678ProTrp: 0.678 ± 0.028	1.153ProTyr: 1.153 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	4.015GlnAla: 4.015 ± 0.064	0.195GlnCys: 0.195 ± 0.016	1.757GlnAsp: 1.757 ± 0.048	1.601GlnGlu: 1.601 ± 0.045	0.961GlnPhe: 0.961 ± 0.032	2.683GlnGly: 2.683 ± 0.053	0.579GlnHis: 0.579 ± 0.024	1.684GlnIle: 1.684 ± 0.041	0.793GlnLys: 0.793 ± 0.033	2.601GlnLeu: 2.601 ± 0.056	0.993GlnMet: 0.993 ± 0.034	0.711GlnAsn: 0.711 ± 0.024	1.645GlnPro: 1.645 ± 0.045	0.997GlnGln: 0.997 ± 0.031	2.138GlnArg: 2.138 ± 0.051	1.551GlnSer: 1.551 ± 0.038	1.671GlnThr: 1.671 ± 0.041	2.349GlnVal: 2.349 ± 0.048	0.374GlnTrp: 0.374 ± 0.016	0.528GlnTyr: 0.528 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	9.412ArgAla: 9.412 ± 0.108	0.514ArgCys: 0.514 ± 0.022	5.034ArgAsp: 5.034 ± 0.074	4.048ArgGlu: 4.048 ± 0.073	2.919ArgPhe: 2.919 ± 0.062	5.318ArgGly: 5.318 ± 0.076	1.808ArgHis: 1.808 ± 0.047	3.897ArgIle: 3.897 ± 0.069	2.096ArgLys: 2.096 ± 0.052	8.348ArgLeu: 8.348 ± 0.112	2.158ArgMet: 2.158 ± 0.05	1.746ArgAsn: 1.746 ± 0.041	3.734ArgPro: 3.734 ± 0.066	2.347ArgGln: 2.347 ± 0.047	5.603ArgArg: 5.603 ± 0.086	3.145ArgSer: 3.145 ± 0.049	2.966ArgThr: 2.966 ± 0.061	5.167ArgVal: 5.167 ± 0.071	0.974ArgTrp: 0.974 ± 0.032	1.639ArgTyr: 1.639 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	5.491SerAla: 5.491 ± 0.073	0.415SerCys: 0.415 ± 0.019	3.144SerAsp: 3.144 ± 0.055	2.496SerGlu: 2.496 ± 0.057	2.044SerPhe: 2.044 ± 0.048	5.044SerGly: 5.044 ± 0.081	1.066SerHis: 1.066 ± 0.036	2.098SerIle: 2.098 ± 0.044	1.068SerLys: 1.068 ± 0.035	4.725SerLeu: 4.725 ± 0.073	1.222SerMet: 1.222 ± 0.036	1.115SerAsn: 1.115 ± 0.035	2.494SerPro: 2.494 ± 0.052	1.404SerGln: 1.404 ± 0.039	3.238SerArg: 3.238 ± 0.06	2.088SerSer: 2.088 ± 0.046	2.325SerThr: 2.325 ± 0.044	3.6SerVal: 3.6 ± 0.066	0.61SerTrp: 0.61 ± 0.024	1.186SerTyr: 1.186 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.272ThrAla: 6.272 ± 0.081	0.526ThrCys: 0.526 ± 0.024	3.24ThrAsp: 3.24 ± 0.055	3.035ThrGlu: 3.035 ± 0.065	1.81ThrPhe: 1.81 ± 0.044	5.92ThrGly: 5.92 ± 0.079	1.122ThrHis: 1.122 ± 0.037	2.596ThrIle: 2.596 ± 0.056	1.086ThrLys: 1.086 ± 0.035	6.17ThrLeu: 6.17 ± 0.091	1.351ThrMet: 1.351 ± 0.039	1.133ThrAsn: 1.133 ± 0.035	3.591ThrPro: 3.591 ± 0.065	1.488ThrGln: 1.488 ± 0.037	3.896ThrArg: 3.896 ± 0.063	2.358ThrSer: 2.358 ± 0.049	2.719ThrThr: 2.719 ± 0.066	4.228ThrVal: 4.228 ± 0.071	0.707ThrTrp: 0.707 ± 0.027	1.176ThrTyr: 1.176 ± 0.036	0.0ThrXaa: 0.0 ± 0.0
Val	9.234ValAla: 9.234 ± 0.118	0.632ValCys: 0.632 ± 0.027	4.227ValAsp: 4.227 ± 0.069	4.708ValGlu: 4.708 ± 0.075	2.792ValPhe: 2.792 ± 0.052	5.401ValGly: 5.401 ± 0.065	1.435ValHis: 1.435 ± 0.037	4.167ValIle: 4.167 ± 0.07	1.829ValLys: 1.829 ± 0.05	7.546ValLeu: 7.546 ± 0.102	2.197ValMet: 2.197 ± 0.051	1.909ValAsn: 1.909 ± 0.044	3.649ValPro: 3.649 ± 0.052	2.133ValGln: 2.133 ± 0.044	4.212ValArg: 4.212 ± 0.064	3.903ValSer: 3.903 ± 0.068	4.934ValThr: 4.934 ± 0.067	5.619ValVal: 5.619 ± 0.092	0.983ValTrp: 0.983 ± 0.032	1.463ValTyr: 1.463 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.426TrpAla: 1.426 ± 0.038	0.13TrpCys: 0.13 ± 0.012	0.81TrpAsp: 0.81 ± 0.029	0.604TrpGlu: 0.604 ± 0.026	0.557TrpPhe: 0.557 ± 0.028	1.013TrpGly: 1.013 ± 0.032	0.381TrpHis: 0.381 ± 0.017	0.728TrpIle: 0.728 ± 0.027	0.335TrpLys: 0.335 ± 0.019	1.706TrpLeu: 1.706 ± 0.047	0.395TrpMet: 0.395 ± 0.02	0.384TrpAsn: 0.384 ± 0.022	0.78TrpPro: 0.78 ± 0.027	0.722TrpGln: 0.722 ± 0.025	1.253TrpArg: 1.253 ± 0.039	0.71TrpSer: 0.71 ± 0.027	0.75TrpThr: 0.75 ± 0.026	0.928TrpVal: 0.928 ± 0.032	0.241TrpTrp: 0.241 ± 0.014	0.26TrpTyr: 0.26 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.453TyrAla: 2.453 ± 0.052	0.224TyrCys: 0.224 ± 0.014	1.566TyrAsp: 1.566 ± 0.042	1.232TyrGlu: 1.232 ± 0.039	0.851TyrPhe: 0.851 ± 0.028	2.099TyrGly: 2.099 ± 0.05	0.562TyrHis: 0.562 ± 0.023	0.893TyrIle: 0.893 ± 0.03	0.475TyrLys: 0.475 ± 0.02	2.183TyrLeu: 2.183 ± 0.048	0.502TyrMet: 0.502 ± 0.021	0.512TyrAsn: 0.512 ± 0.022	1.043TyrPro: 1.043 ± 0.031	0.628TyrGln: 0.628 ± 0.021	1.643TyrArg: 1.643 ± 0.043	0.978TyrSer: 0.978 ± 0.031	1.116TyrThr: 1.116 ± 0.037	1.43TyrVal: 1.43 ± 0.035	0.362TyrTrp: 0.362 ± 0.019	0.546TyrTyr: 0.546 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3286 proteins (1037509 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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