Apple-associated luteovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4QXI9|A0A2H4QXI9_9LUTE Isoform of A0A2H4QXF1 Coat protein OS=Apple-associated luteovirus OX=2054409 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.37FFDD4 pKa = 4.05EE5 pKa = 6.32LITASIKK12 pKa = 9.51VVRR15 pKa = 11.84DD16 pKa = 5.1FIAYY20 pKa = 9.48IYY22 pKa = 11.1NNLKK26 pKa = 10.24NVYY29 pKa = 9.56KK30 pKa = 10.46RR31 pKa = 11.84FKK33 pKa = 8.11MWLWEE38 pKa = 4.01LQGKK42 pKa = 9.06FSQHH46 pKa = 6.33DD47 pKa = 3.63AFADD51 pKa = 3.42MCYY54 pKa = 10.83GYY56 pKa = 9.68MDD58 pKa = 4.34DD59 pKa = 4.04VEE61 pKa = 4.22EE62 pKa = 5.18FEE64 pKa = 5.77WEE66 pKa = 4.3LQTQLDD72 pKa = 3.91NAEE75 pKa = 4.26TQMLLAEE82 pKa = 4.39KK83 pKa = 10.03HH84 pKa = 5.27LQAMLKK90 pKa = 10.14APKK93 pKa = 8.36ITGWPTPTRR102 pKa = 11.84PAGAIPAVPVKK113 pKa = 10.09TRR115 pKa = 11.84TLRR118 pKa = 11.84EE119 pKa = 3.7LADD122 pKa = 3.65EE123 pKa = 4.2VRR125 pKa = 11.84ARR127 pKa = 11.84QGIDD131 pKa = 2.91TGGHH135 pKa = 5.09ATMDD139 pKa = 3.81VPSPSAIPLPDD150 pKa = 4.6LSDD153 pKa = 3.29EE154 pKa = 4.29DD155 pKa = 4.24LRR157 pKa = 11.84FILSDD162 pKa = 3.7TIEE165 pKa = 4.4TDD167 pKa = 3.33PEE169 pKa = 4.33HH170 pKa = 7.39VVVFPPGYY178 pKa = 8.94EE179 pKa = 3.93EE180 pKa = 4.07KK181 pKa = 10.44EE182 pKa = 4.06PRR184 pKa = 11.84VLEE187 pKa = 4.46LPSSPKK193 pKa = 9.91EE194 pKa = 4.01HH195 pKa = 7.28IIQEE199 pKa = 4.56PIATLGQAYY208 pKa = 8.11TPEE211 pKa = 6.11DD212 pKa = 3.43IEE214 pKa = 4.11KK215 pKa = 10.76ARR217 pKa = 11.84AEE219 pKa = 4.14FARR222 pKa = 11.84ARR224 pKa = 11.84LDD226 pKa = 3.36YY227 pKa = 9.47TVCLEE232 pKa = 4.21EE233 pKa = 4.27TLDD236 pKa = 3.72AYY238 pKa = 10.06EE239 pKa = 4.39AEE241 pKa = 4.57KK242 pKa = 10.99GDD244 pKa = 3.93GYY246 pKa = 10.91FGRR249 pKa = 11.84FFNTVAHH256 pKa = 6.32RR257 pKa = 11.84MAYY260 pKa = 9.21IKK262 pKa = 10.31KK263 pKa = 9.46CKK265 pKa = 9.77ARR267 pKa = 11.84RR268 pKa = 11.84AKK270 pKa = 9.95TDD272 pKa = 3.67LLAHH276 pKa = 6.99KK277 pKa = 9.79ISNRR281 pKa = 11.84VRR283 pKa = 11.84SAPSVAEE290 pKa = 3.77FHH292 pKa = 6.55SLCNVVEE299 pKa = 4.37EE300 pKa = 4.45PTGEE304 pKa = 4.01YY305 pKa = 10.84KK306 pKa = 10.69NIHH309 pKa = 6.25KK310 pKa = 10.74DD311 pKa = 2.85EE312 pKa = 4.59GEE314 pKa = 4.0NLRR317 pKa = 11.84EE318 pKa = 3.87EE319 pKa = 4.33VMKK322 pKa = 10.03VVRR325 pKa = 11.84YY326 pKa = 9.58IKK328 pKa = 9.57PDD330 pKa = 3.25KK331 pKa = 10.84VRR333 pKa = 11.84EE334 pKa = 3.85AQQYY338 pKa = 7.5IRR340 pKa = 11.84HH341 pKa = 5.08YY342 pKa = 11.12VRR344 pKa = 11.84AKK346 pKa = 10.51NNRR349 pKa = 11.84LSADD353 pKa = 3.73EE354 pKa = 4.35VSSATINRR362 pKa = 11.84YY363 pKa = 6.97VQQFAEE369 pKa = 4.41DD370 pKa = 3.77NKK372 pKa = 10.92LSLSSTHH379 pKa = 6.24LLIRR383 pKa = 11.84AALTMVPVITKK394 pKa = 9.8EE395 pKa = 3.98DD396 pKa = 3.61MMTAMVIHH404 pKa = 6.66GPAARR409 pKa = 11.84KK410 pKa = 9.28ARR412 pKa = 11.84SDD414 pKa = 3.39LSTLEE419 pKa = 4.5GGDD422 pKa = 3.64FF423 pKa = 3.8

Molecular weight: 48.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4QXD9|A0A2H4QXD9_9LUTE Movement protein OS=Apple-associated luteovirus OX=2054409 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84AVDD5 pKa = 3.44SWRR8 pKa = 11.84VTHH11 pKa = 6.19VKK13 pKa = 10.43NMPPPRR19 pKa = 11.84GVDD22 pKa = 3.43CLGLRR27 pKa = 11.84HH28 pKa = 6.95RR29 pKa = 11.84ITSVHH34 pKa = 5.54VLGLSEE40 pKa = 4.22VLWVVEE46 pKa = 4.28ACLHH50 pKa = 5.64IHH52 pKa = 4.73TCRR55 pKa = 11.84WKK57 pKa = 9.75GSGGPVHH64 pKa = 6.35TLHH67 pKa = 6.37GVVRR71 pKa = 11.84IPKK74 pKa = 8.42RR75 pKa = 11.84TSSDD79 pKa = 3.48SLTMNQSTDD88 pKa = 2.83CGYY91 pKa = 11.06SRR93 pKa = 11.84RR94 pKa = 3.99

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

2851

45

951

356.4

40.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.155 ± 0.808

1.684 ± 0.466

5.367 ± 0.935

5.542 ± 1.124

3.718 ± 0.387

5.928 ± 0.424

2.21 ± 0.415

4.981 ± 0.51

5.437 ± 0.592

8.909 ± 0.748

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.463

3.578 ± 0.387

5.893 ± 0.547

3.297 ± 0.17

8.313 ± 0.886

8.839 ± 1.584

5.787 ± 0.762

6.559 ± 0.662

1.263 ± 0.18

2.911 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski