Amino acid dipepetide frequency for Pig stool associated circular ssDNA virus GER2011

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.155AlaAla: 2.155 ± 1.618
3.233AlaCys: 3.233 ± 1.564
8.621AlaAsp: 8.621 ± 1.755
0.0AlaGlu: 0.0 ± 0.0
2.155AlaPhe: 2.155 ± 1.832
3.233AlaGly: 3.233 ± 2.471
0.0AlaHis: 0.0 ± 0.0
1.078AlaIle: 1.078 ± 0.809
1.078AlaLys: 1.078 ± 0.809
5.388AlaLeu: 5.388 ± 1.563
4.31AlaMet: 4.31 ± 1.14
3.233AlaAsn: 3.233 ± 1.697
3.233AlaPro: 3.233 ± 1.376
1.078AlaGln: 1.078 ± 1.318
0.0AlaArg: 0.0 ± 0.0
2.155AlaSer: 2.155 ± 1.282
4.31AlaThr: 4.31 ± 1.65
4.31AlaVal: 4.31 ± 1.671
1.078AlaTrp: 1.078 ± 0.916
1.078AlaTyr: 1.078 ± 1.329
0.0AlaXaa: 0.0 ± 0.0
Cys
1.078CysAla: 1.078 ± 0.809
0.0CysCys: 0.0 ± 0.0
1.078CysAsp: 1.078 ± 0.809
1.078CysGlu: 1.078 ± 0.916
0.0CysPhe: 0.0 ± 0.0
0.0CysGly: 0.0 ± 0.0
0.0CysHis: 0.0 ± 0.0
0.0CysIle: 0.0 ± 0.0
0.0CysLys: 0.0 ± 0.0
2.155CysLeu: 2.155 ± 2.326
0.0CysMet: 0.0 ± 0.0
1.078CysAsn: 1.078 ± 0.916
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
0.0CysArg: 0.0 ± 0.0
3.233CysSer: 3.233 ± 2.008
2.155CysThr: 2.155 ± 2.326
1.078CysVal: 1.078 ± 0.916
0.0CysTrp: 0.0 ± 0.0
1.078CysTyr: 1.078 ± 0.809
0.0CysXaa: 0.0 ± 0.0
Asp
2.155AspAla: 2.155 ± 1.282
0.0AspCys: 0.0 ± 0.0
3.233AspAsp: 3.233 ± 1.376
2.155AspGlu: 2.155 ± 1.832
2.155AspPhe: 2.155 ± 0.843
3.233AspGly: 3.233 ± 1.369
1.078AspHis: 1.078 ± 1.329
2.155AspIle: 2.155 ± 0.843
2.155AspLys: 2.155 ± 0.843
7.543AspLeu: 7.543 ± 1.68
3.233AspMet: 3.233 ± 2.221
1.078AspAsn: 1.078 ± 0.809
2.155AspPro: 2.155 ± 1.618
1.078AspGln: 1.078 ± 1.318
3.233AspArg: 3.233 ± 2.748
1.078AspSer: 1.078 ± 0.809
5.388AspThr: 5.388 ± 2.134
7.543AspVal: 7.543 ± 4.139
4.31AspTrp: 4.31 ± 3.003
4.31AspTyr: 4.31 ± 1.671
0.0AspXaa: 0.0 ± 0.0
Glu
1.078GluAla: 1.078 ± 0.809
0.0GluCys: 0.0 ± 0.0
0.0GluAsp: 0.0 ± 0.0
2.155GluGlu: 2.155 ± 1.832
2.155GluPhe: 2.155 ± 0.843
2.155GluGly: 2.155 ± 0.843
0.0GluHis: 0.0 ± 0.0
4.31GluIle: 4.31 ± 2.093
3.233GluLys: 3.233 ± 2.11
3.233GluLeu: 3.233 ± 1.564
0.0GluMet: 0.0 ± 0.0
1.078GluAsn: 1.078 ± 0.916
1.078GluPro: 1.078 ± 0.809
3.233GluGln: 3.233 ± 1.692
3.233GluArg: 3.233 ± 2.008
2.155GluSer: 2.155 ± 1.448
3.233GluThr: 3.233 ± 1.16
2.155GluVal: 2.155 ± 1.832
1.078GluTrp: 1.078 ± 0.916
2.155GluTyr: 2.155 ± 1.832
0.0GluXaa: 0.0 ± 0.0
Phe
0.0PheAla: 0.0 ± 0.0
0.0PheCys: 0.0 ± 0.0
3.233PheAsp: 3.233 ± 1.376
3.233PheGlu: 3.233 ± 2.748
2.155PhePhe: 2.155 ± 1.618
3.233PheGly: 3.233 ± 1.004
1.078PheHis: 1.078 ± 1.329
0.0PheIle: 0.0 ± 0.0
3.233PheLys: 3.233 ± 2.544
3.233PheLeu: 3.233 ± 2.55
1.078PheMet: 1.078 ± 0.809
3.233PheAsn: 3.233 ± 1.376
2.155PhePro: 2.155 ± 1.668
1.078PheGln: 1.078 ± 0.809
6.466PheArg: 6.466 ± 3.172
3.233PheSer: 3.233 ± 1.174
3.233PheThr: 3.233 ± 1.448
3.233PheVal: 3.233 ± 1.697
1.078PheTrp: 1.078 ± 0.809
2.155PheTyr: 2.155 ± 0.843
0.0PheXaa: 0.0 ± 0.0
Gly
4.31GlyAla: 4.31 ± 2.435
1.078GlyCys: 1.078 ± 0.916
2.155GlyAsp: 2.155 ± 1.282
1.078GlyGlu: 1.078 ± 1.329
1.078GlyPhe: 1.078 ± 1.163
4.31GlyGly: 4.31 ± 2.727
1.078GlyHis: 1.078 ± 0.809
4.31GlyIle: 4.31 ± 1.864
5.388GlyLys: 5.388 ± 2.134
10.776GlyLeu: 10.776 ± 3.18
0.0GlyMet: 0.0 ± 0.0
1.078GlyAsn: 1.078 ± 0.916
1.078GlyPro: 1.078 ± 0.809
0.0GlyGln: 0.0 ± 0.0
5.388GlyArg: 5.388 ± 2.685
1.078GlySer: 1.078 ± 1.163
4.31GlyThr: 4.31 ± 2.406
2.155GlyVal: 2.155 ± 0.843
3.233GlyTrp: 3.233 ± 2.471
3.233GlyTyr: 3.233 ± 2.748
0.0GlyXaa: 0.0 ± 0.0
His
0.0HisAla: 0.0 ± 0.0
0.0HisCys: 0.0 ± 0.0
0.0HisAsp: 0.0 ± 0.0
0.0HisGlu: 0.0 ± 0.0
0.0HisPhe: 0.0 ± 0.0
0.0HisGly: 0.0 ± 0.0
0.0HisHis: 0.0 ± 0.0
1.078HisIle: 1.078 ± 0.916
0.0HisLys: 0.0 ± 0.0
1.078HisLeu: 1.078 ± 1.163
1.078HisMet: 1.078 ± 0.916
1.078HisAsn: 1.078 ± 1.318
1.078HisPro: 1.078 ± 1.163
1.078HisGln: 1.078 ± 0.809
4.31HisArg: 4.31 ± 2.482
1.078HisSer: 1.078 ± 1.329
2.155HisThr: 2.155 ± 1.341
1.078HisVal: 1.078 ± 0.916
0.0HisTrp: 0.0 ± 0.0
1.078HisTyr: 1.078 ± 0.916
0.0HisXaa: 0.0 ± 0.0
Ile
2.155IleAla: 2.155 ± 0.843
1.078IleCys: 1.078 ± 1.163
2.155IleAsp: 2.155 ± 1.501
5.388IleGlu: 5.388 ± 1.563
0.0IlePhe: 0.0 ± 0.0
5.388IleGly: 5.388 ± 2.89
1.078IleHis: 1.078 ± 0.809
3.233IleIle: 3.233 ± 2.027
0.0IleLys: 0.0 ± 0.0
6.466IleLeu: 6.466 ± 3.057
2.155IleMet: 2.155 ± 1.618
3.233IleAsn: 3.233 ± 1.697
3.233IlePro: 3.233 ± 1.16
1.078IleGln: 1.078 ± 1.329
3.233IleArg: 3.233 ± 2.783
6.466IleSer: 6.466 ± 2.385
2.155IleThr: 2.155 ± 1.832
2.155IleVal: 2.155 ± 1.341
1.078IleTrp: 1.078 ± 0.916
0.0IleTyr: 0.0 ± 0.0
0.0IleXaa: 0.0 ± 0.0
Lys
3.233LysAla: 3.233 ± 1.174
0.0LysCys: 0.0 ± 0.0
3.233LysAsp: 3.233 ± 1.174
0.0LysGlu: 0.0 ± 0.0
1.078LysPhe: 1.078 ± 0.809
2.155LysGly: 2.155 ± 0.843
2.155LysHis: 2.155 ± 1.448
0.0LysIle: 0.0 ± 0.0
1.078LysLys: 1.078 ± 0.809
7.543LysLeu: 7.543 ± 2.016
1.078LysMet: 1.078 ± 0.809
6.466LysAsn: 6.466 ± 2.349
0.0LysPro: 0.0 ± 0.0
0.0LysGln: 0.0 ± 0.0
2.155LysArg: 2.155 ± 1.832
3.233LysSer: 3.233 ± 2.544
4.31LysThr: 4.31 ± 1.38
4.31LysVal: 4.31 ± 1.38
0.0LysTrp: 0.0 ± 0.0
3.233LysTyr: 3.233 ± 2.672
0.0LysXaa: 0.0 ± 0.0
Leu
4.31LeuAla: 4.31 ± 2.003
2.155LeuCys: 2.155 ± 1.361
4.31LeuAsp: 4.31 ± 2.173
3.233LeuGlu: 3.233 ± 2.008
2.155LeuPhe: 2.155 ± 1.136
6.466LeuGly: 6.466 ± 1.121
2.155LeuHis: 2.155 ± 1.638
5.388LeuIle: 5.388 ± 2.319
5.388LeuLys: 5.388 ± 2.34
9.698LeuLeu: 9.698 ± 6.366
0.0LeuMet: 0.0 ± 0.0
3.233LeuAsn: 3.233 ± 2.427
5.388LeuPro: 5.388 ± 1.072
7.543LeuGln: 7.543 ± 2.549
4.31LeuArg: 4.31 ± 3.463
12.931LeuSer: 12.931 ± 2.202
5.388LeuThr: 5.388 ± 3.68
7.543LeuVal: 7.543 ± 2.086
2.155LeuTrp: 2.155 ± 0.843
7.543LeuTyr: 7.543 ± 3.425
0.0LeuXaa: 0.0 ± 0.0
Met
1.078MetAla: 1.078 ± 0.809
0.0MetCys: 0.0 ± 0.0
4.31MetAsp: 4.31 ± 2.435
1.078MetGlu: 1.078 ± 0.809
1.078MetPhe: 1.078 ± 0.809
2.155MetGly: 2.155 ± 1.282
0.0MetHis: 0.0 ± 0.0
0.0MetIle: 0.0 ± 0.0
1.078MetLys: 1.078 ± 0.916
1.078MetLeu: 1.078 ± 0.809
0.0MetMet: 0.0 ± 0.0
0.0MetAsn: 0.0 ± 0.0
4.31MetPro: 4.31 ± 1.38
3.233MetGln: 3.233 ± 2.427
2.155MetArg: 2.155 ± 1.618
1.078MetSer: 1.078 ± 1.163
2.155MetThr: 2.155 ± 2.326
3.233MetVal: 3.233 ± 2.427
0.0MetTrp: 0.0 ± 0.0
1.078MetTyr: 1.078 ± 0.916
0.0MetXaa: 0.0 ± 0.0
Asn
2.155AsnAla: 2.155 ± 1.501
1.078AsnCys: 1.078 ± 0.809
3.233AsnAsp: 3.233 ± 1.564
0.0AsnGlu: 0.0 ± 0.0
5.388AsnPhe: 5.388 ± 1.386
4.31AsnGly: 4.31 ± 1.452
1.078AsnHis: 1.078 ± 0.916
3.233AsnIle: 3.233 ± 1.697
2.155AsnLys: 2.155 ± 2.635
0.0AsnLeu: 0.0 ± 0.0
0.0AsnMet: 0.0 ± 0.0
1.078AsnAsn: 1.078 ± 0.809
2.155AsnPro: 2.155 ± 1.341
0.0AsnGln: 0.0 ± 0.0
4.31AsnArg: 4.31 ± 2.321
5.388AsnSer: 5.388 ± 2.134
3.233AsnThr: 3.233 ± 2.427
0.0AsnVal: 0.0 ± 0.0
0.0AsnTrp: 0.0 ± 0.0
1.078AsnTyr: 1.078 ± 0.916
0.0AsnXaa: 0.0 ± 0.0
Pro
4.31ProAla: 4.31 ± 2.321
3.233ProCys: 3.233 ± 2.152
2.155ProAsp: 2.155 ± 0.843
6.466ProGlu: 6.466 ± 2.115
3.233ProPhe: 3.233 ± 1.16
0.0ProGly: 0.0 ± 0.0
1.078ProHis: 1.078 ± 0.916
3.233ProIle: 3.233 ± 1.448
2.155ProLys: 2.155 ± 2.657
6.466ProLeu: 6.466 ± 2.733
2.155ProMet: 2.155 ± 1.136
1.078ProAsn: 1.078 ± 0.809
2.155ProPro: 2.155 ± 0.843
2.155ProGln: 2.155 ± 1.618
4.31ProArg: 4.31 ± 1.687
5.388ProSer: 5.388 ± 3.036
1.078ProThr: 1.078 ± 0.916
1.078ProVal: 1.078 ± 1.318
0.0ProTrp: 0.0 ± 0.0
0.0ProTyr: 0.0 ± 0.0
0.0ProXaa: 0.0 ± 0.0
Gln
2.155GlnAla: 2.155 ± 1.282
0.0GlnCys: 0.0 ± 0.0
3.233GlnAsp: 3.233 ± 1.376
1.078GlnGlu: 1.078 ± 1.318
4.31GlnPhe: 4.31 ± 1.38
2.155GlnGly: 2.155 ± 1.618
1.078GlnHis: 1.078 ± 1.163
2.155GlnIle: 2.155 ± 1.282
0.0GlnLys: 0.0 ± 0.0
5.388GlnLeu: 5.388 ± 1.532
1.078GlnMet: 1.078 ± 0.809
1.078GlnAsn: 1.078 ± 0.916
0.0GlnPro: 0.0 ± 0.0
1.078GlnGln: 1.078 ± 1.318
4.31GlnArg: 4.31 ± 1.97
2.155GlnSer: 2.155 ± 1.282
3.233GlnThr: 3.233 ± 2.427
4.31GlnVal: 4.31 ± 1.38
1.078GlnTrp: 1.078 ± 1.163
2.155GlnTyr: 2.155 ± 1.668
0.0GlnXaa: 0.0 ± 0.0
Arg
3.233ArgAla: 3.233 ± 2.11
0.0ArgCys: 0.0 ± 0.0
4.31ArgAsp: 4.31 ± 2.885
1.078ArgGlu: 1.078 ± 0.916
3.233ArgPhe: 3.233 ± 1.593
4.31ArgGly: 4.31 ± 1.604
2.155ArgHis: 2.155 ± 1.361
7.543ArgIle: 7.543 ± 1.88
1.078ArgLys: 1.078 ± 0.916
5.388ArgLeu: 5.388 ± 2.646
2.155ArgMet: 2.155 ± 1.136
2.155ArgAsn: 2.155 ± 1.448
3.233ArgPro: 3.233 ± 2.027
2.155ArgGln: 2.155 ± 1.897
3.233ArgArg: 3.233 ± 2.55
7.543ArgSer: 7.543 ± 3.8
3.233ArgThr: 3.233 ± 2.36
2.155ArgVal: 2.155 ± 1.832
1.078ArgTrp: 1.078 ± 1.163
2.155ArgTyr: 2.155 ± 1.832
0.0ArgXaa: 0.0 ± 0.0
Ser
4.31SerAla: 4.31 ± 2.348
1.078SerCys: 1.078 ± 1.163
6.466SerAsp: 6.466 ± 4.921
3.233SerGlu: 3.233 ± 1.004
6.466SerPhe: 6.466 ± 4.943
5.388SerGly: 5.388 ± 1.482
1.078SerHis: 1.078 ± 0.916
3.233SerIle: 3.233 ± 1.16
6.466SerLys: 6.466 ± 3.385
8.621SerLeu: 8.621 ± 3.082
4.31SerMet: 4.31 ± 3.236
4.31SerAsn: 4.31 ± 1.452
6.466SerPro: 6.466 ± 1.517
5.388SerGln: 5.388 ± 3.288
1.078SerArg: 1.078 ± 1.163
6.466SerSer: 6.466 ± 4.335
10.776SerThr: 10.776 ± 6.29
7.543SerVal: 7.543 ± 2.185
2.155SerTrp: 2.155 ± 1.361
0.0SerTyr: 0.0 ± 0.0
0.0SerXaa: 0.0 ± 0.0
Thr
4.31ThrAla: 4.31 ± 1.38
0.0ThrCys: 0.0 ± 0.0
4.31ThrAsp: 4.31 ± 2.406
2.155ThrGlu: 2.155 ± 1.618
0.0ThrPhe: 0.0 ± 0.0
2.155ThrGly: 2.155 ± 1.341
0.0ThrHis: 0.0 ± 0.0
5.388ThrIle: 5.388 ± 2.252
2.155ThrLys: 2.155 ± 1.341
5.388ThrLeu: 5.388 ± 2.239
1.078ThrMet: 1.078 ± 0.809
0.0ThrAsn: 0.0 ± 0.0
8.621ThrPro: 8.621 ± 2.288
4.31ThrGln: 4.31 ± 2.093
4.31ThrArg: 4.31 ± 3.463
12.931ThrSer: 12.931 ± 3.518
7.543ThrThr: 7.543 ± 3.22
7.543ThrVal: 7.543 ± 6.712
1.078ThrTrp: 1.078 ± 0.809
1.078ThrTyr: 1.078 ± 0.809
0.0ThrXaa: 0.0 ± 0.0
Val
5.388ValAla: 5.388 ± 2.134
0.0ValCys: 0.0 ± 0.0
1.078ValAsp: 1.078 ± 0.809
1.078ValGlu: 1.078 ± 0.809
5.388ValPhe: 5.388 ± 1.482
3.233ValGly: 3.233 ± 1.691
1.078ValHis: 1.078 ± 1.163
2.155ValIle: 2.155 ± 1.897
2.155ValLys: 2.155 ± 1.282
7.543ValLeu: 7.543 ± 1.804
4.31ValMet: 4.31 ± 1.147
4.31ValAsn: 4.31 ± 3.236
3.233ValPro: 3.233 ± 1.369
3.233ValGln: 3.233 ± 1.564
3.233ValArg: 3.233 ± 1.564
9.698ValSer: 9.698 ± 3.431
3.233ValThr: 3.233 ± 1.376
1.078ValVal: 1.078 ± 0.809
1.078ValTrp: 1.078 ± 0.916
3.233ValTyr: 3.233 ± 1.564
0.0ValXaa: 0.0 ± 0.0
Trp
2.155TrpAla: 2.155 ± 1.832
0.0TrpCys: 0.0 ± 0.0
1.078TrpAsp: 1.078 ± 0.916
1.078TrpGlu: 1.078 ± 0.916
2.155TrpPhe: 2.155 ± 1.638
2.155TrpGly: 2.155 ± 1.136
0.0TrpHis: 0.0 ± 0.0
0.0TrpIle: 0.0 ± 0.0
4.31TrpLys: 4.31 ± 1.346
1.078TrpLeu: 1.078 ± 1.163
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
0.0TrpPro: 0.0 ± 0.0
1.078TrpGln: 1.078 ± 0.916
0.0TrpArg: 0.0 ± 0.0
2.155TrpSer: 2.155 ± 1.501
1.078TrpThr: 1.078 ± 0.809
0.0TrpVal: 0.0 ± 0.0
0.0TrpTrp: 0.0 ± 0.0
2.155TrpTyr: 2.155 ± 1.501
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.155TyrAla: 2.155 ± 0.843
1.078TyrCys: 1.078 ± 0.916
1.078TyrAsp: 1.078 ± 0.809
2.155TyrGlu: 2.155 ± 1.832
2.155TyrPhe: 2.155 ± 1.832
1.078TyrGly: 1.078 ± 0.809
0.0TyrHis: 0.0 ± 0.0
3.233TyrIle: 3.233 ± 2.11
2.155TyrLys: 2.155 ± 0.843
3.233TyrLeu: 3.233 ± 2.11
0.0TyrMet: 0.0 ± 1.11
1.078TyrAsn: 1.078 ± 1.318
2.155TyrPro: 2.155 ± 1.282
3.233TyrGln: 3.233 ± 1.564
2.155TyrArg: 2.155 ± 1.282
5.388TyrSer: 5.388 ± 1.445
2.155TyrThr: 2.155 ± 1.668
3.233TyrVal: 3.233 ± 1.564
0.0TyrTrp: 0.0 ± 0.0
2.155TyrTyr: 2.155 ± 1.618
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5 proteins (929 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski