Amino acid dipepetide frequency for Paenibacillus sp. Root444D2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.526AlaAla: 7.526 ± 0.072	0.678AlaCys: 0.678 ± 0.018	3.836AlaAsp: 3.836 ± 0.046	4.781AlaGlu: 4.781 ± 0.051	3.37AlaPhe: 3.37 ± 0.045	5.837AlaGly: 5.837 ± 0.062	1.39AlaHis: 1.39 ± 0.023	5.681AlaIle: 5.681 ± 0.052	4.386AlaLys: 4.386 ± 0.047	7.693AlaLeu: 7.693 ± 0.07	2.176AlaMet: 2.176 ± 0.031	2.886AlaAsn: 2.886 ± 0.045	2.52AlaPro: 2.52 ± 0.038	2.617AlaGln: 2.617 ± 0.038	2.954AlaArg: 2.954 ± 0.036	4.986AlaSer: 4.986 ± 0.051	3.716AlaThr: 3.716 ± 0.047	5.689AlaVal: 5.689 ± 0.059	0.951AlaTrp: 0.951 ± 0.02	2.624AlaTyr: 2.624 ± 0.037	0.002AlaXaa: 0.002 ± 0.001
Cys	0.475CysAla: 0.475 ± 0.015	0.104CysCys: 0.104 ± 0.007	0.375CysAsp: 0.375 ± 0.013	0.44CysGlu: 0.44 ± 0.016	0.355CysPhe: 0.355 ± 0.015	0.69CysGly: 0.69 ± 0.017	0.201CysHis: 0.201 ± 0.008	0.517CysIle: 0.517 ± 0.015	0.37CysLys: 0.37 ± 0.013	0.758CysLeu: 0.758 ± 0.016	0.23CysMet: 0.23 ± 0.009	0.276CysAsn: 0.276 ± 0.011	0.34CysPro: 0.34 ± 0.013	0.217CysGln: 0.217 ± 0.01	0.362CysArg: 0.362 ± 0.014	0.603CysSer: 0.603 ± 0.016	0.419CysThr: 0.419 ± 0.015	0.454CysVal: 0.454 ± 0.012	0.097CysTrp: 0.097 ± 0.006	0.278CysTyr: 0.278 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	3.745AspAla: 3.745 ± 0.049	0.377AspCys: 0.377 ± 0.014	2.308AspAsp: 2.308 ± 0.036	3.525AspGlu: 3.525 ± 0.04	2.276AspPhe: 2.276 ± 0.03	3.873AspGly: 3.873 ± 0.049	1.216AspHis: 1.216 ± 0.024	3.59AspIle: 3.59 ± 0.045	2.85AspLys: 2.85 ± 0.041	4.98AspLeu: 4.98 ± 0.053	1.357AspMet: 1.357 ± 0.023	1.885AspAsn: 1.885 ± 0.03	2.29AspPro: 2.29 ± 0.04	2.042AspGln: 2.042 ± 0.031	2.492AspArg: 2.492 ± 0.033	2.849AspSer: 2.849 ± 0.037	2.483AspThr: 2.483 ± 0.036	3.533AspVal: 3.533 ± 0.046	0.901AspTrp: 0.901 ± 0.022	2.082AspTyr: 2.082 ± 0.034	0.002AspXaa: 0.002 ± 0.001
Glu	5.342GluAla: 5.342 ± 0.049	0.361GluCys: 0.361 ± 0.012	2.964GluAsp: 2.964 ± 0.044	4.7GluGlu: 4.7 ± 0.053	2.25GluPhe: 2.25 ± 0.032	4.037GluGly: 4.037 ± 0.05	1.518GluHis: 1.518 ± 0.03	4.438GluIle: 4.438 ± 0.05	4.086GluLys: 4.086 ± 0.05	6.779GluLeu: 6.779 ± 0.064	1.897GluMet: 1.897 ± 0.029	2.531GluAsn: 2.531 ± 0.035	2.044GluPro: 2.044 ± 0.035	3.427GluGln: 3.427 ± 0.04	3.444GluArg: 3.444 ± 0.042	3.552GluSer: 3.552 ± 0.045	3.303GluThr: 3.303 ± 0.036	4.34GluVal: 4.34 ± 0.05	0.935GluTrp: 0.935 ± 0.021	1.83GluTyr: 1.83 ± 0.025	0.002GluXaa: 0.002 ± 0.001
Phe	3.175PheAla: 3.175 ± 0.037	0.36PheCys: 0.36 ± 0.013	2.334PheAsp: 2.334 ± 0.036	2.492PheGlu: 2.492 ± 0.037	2.034PhePhe: 2.034 ± 0.037	3.232PheGly: 3.232 ± 0.039	1.046PheHis: 1.046 ± 0.022	3.399PheIle: 3.399 ± 0.047	2.229PheLys: 2.229 ± 0.033	4.189PheLeu: 4.189 ± 0.057	1.21PheMet: 1.21 ± 0.024	1.782PheAsn: 1.782 ± 0.034	1.785PhePro: 1.785 ± 0.029	1.666PheGln: 1.666 ± 0.026	1.948PheArg: 1.948 ± 0.03	2.97PheSer: 2.97 ± 0.04	2.637PheThr: 2.637 ± 0.032	3.099PheVal: 3.099 ± 0.038	0.564PheTrp: 0.564 ± 0.017	1.64PheTyr: 1.64 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	5.094GlyAla: 5.094 ± 0.061	0.719GlyCys: 0.719 ± 0.02	3.292GlyAsp: 3.292 ± 0.041	4.046GlyGlu: 4.046 ± 0.041	3.35GlyPhe: 3.35 ± 0.039	4.941GlyGly: 4.941 ± 0.064	1.524GlyHis: 1.524 ± 0.028	5.864GlyIle: 5.864 ± 0.053	4.563GlyLys: 4.563 ± 0.043	7.055GlyLeu: 7.055 ± 0.069	2.213GlyMet: 2.213 ± 0.03	2.894GlyAsn: 2.894 ± 0.043	1.904GlyPro: 1.904 ± 0.034	2.516GlyGln: 2.516 ± 0.036	3.012GlyArg: 3.012 ± 0.043	4.586GlySer: 4.586 ± 0.048	4.363GlyThr: 4.363 ± 0.054	5.088GlyVal: 5.088 ± 0.048	1.109GlyTrp: 1.109 ± 0.026	2.828GlyTyr: 2.828 ± 0.039	0.001GlyXaa: 0.001 ± 0.001
His	1.564HisAla: 1.564 ± 0.028	0.198HisCys: 0.198 ± 0.009	1.023HisAsp: 1.023 ± 0.022	1.262HisGlu: 1.262 ± 0.027	1.18HisPhe: 1.18 ± 0.024	1.486HisGly: 1.486 ± 0.025	0.644HisHis: 0.644 ± 0.017	1.561HisIle: 1.561 ± 0.027	0.953HisLys: 0.953 ± 0.02	2.319HisLeu: 2.319 ± 0.037	0.586HisMet: 0.586 ± 0.017	0.75HisAsn: 0.75 ± 0.022	1.199HisPro: 1.199 ± 0.024	0.87HisGln: 0.87 ± 0.024	1.06HisArg: 1.06 ± 0.022	1.243HisSer: 1.243 ± 0.025	1.133HisThr: 1.133 ± 0.023	1.51HisVal: 1.51 ± 0.027	0.338HisTrp: 0.338 ± 0.012	0.928HisTyr: 0.928 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	5.867IleAla: 5.867 ± 0.051	0.659IleCys: 0.659 ± 0.018	3.885IleAsp: 3.885 ± 0.038	4.532IleGlu: 4.532 ± 0.052	2.703IlePhe: 2.703 ± 0.039	5.478IleGly: 5.478 ± 0.06	1.703IleHis: 1.703 ± 0.028	4.885IleIle: 4.885 ± 0.057	3.42IleLys: 3.42 ± 0.039	6.458IleLeu: 6.458 ± 0.072	1.767IleMet: 1.767 ± 0.028	2.694IleAsn: 2.694 ± 0.035	3.357IlePro: 3.357 ± 0.041	2.966IleGln: 2.966 ± 0.041	3.787IleArg: 3.787 ± 0.047	5.056IleSer: 5.056 ± 0.056	4.276IleThr: 4.276 ± 0.049	5.36IleVal: 5.36 ± 0.051	0.816IleTrp: 0.816 ± 0.019	2.328IleTyr: 2.328 ± 0.034	0.001IleXaa: 0.001 ± 0.001
Lys	4.434LysAla: 4.434 ± 0.045	0.242LysCys: 0.242 ± 0.01	3.228LysAsp: 3.228 ± 0.043	4.547LysGlu: 4.547 ± 0.05	1.863LysPhe: 1.863 ± 0.029	3.856LysGly: 3.856 ± 0.044	1.241LysHis: 1.241 ± 0.025	3.493LysIle: 3.493 ± 0.051	3.776LysLys: 3.776 ± 0.053	5.741LysLeu: 5.741 ± 0.06	1.849LysMet: 1.849 ± 0.031	2.369LysAsn: 2.369 ± 0.033	2.361LysPro: 2.361 ± 0.035	2.841LysGln: 2.841 ± 0.038	2.634LysArg: 2.634 ± 0.034	3.329LysSer: 3.329 ± 0.038	3.04LysThr: 3.04 ± 0.037	3.994LysVal: 3.994 ± 0.049	0.822LysTrp: 0.822 ± 0.018	1.898LysTyr: 1.898 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	7.621LeuAla: 7.621 ± 0.062	0.794LeuCys: 0.794 ± 0.018	5.086LeuAsp: 5.086 ± 0.048	6.004LeuGlu: 6.004 ± 0.058	4.828LeuPhe: 4.828 ± 0.061	6.632LeuGly: 6.632 ± 0.064	2.177LeuHis: 2.177 ± 0.03	7.092LeuIle: 7.092 ± 0.079	5.63LeuLys: 5.63 ± 0.054	10.83LeuLeu: 10.83 ± 0.113	2.65LeuMet: 2.65 ± 0.035	4.028LeuAsn: 4.028 ± 0.042	4.492LeuPro: 4.492 ± 0.05	4.354LeuGln: 4.354 ± 0.046	4.597LeuArg: 4.597 ± 0.057	7.18LeuSer: 7.18 ± 0.066	5.96LeuThr: 5.96 ± 0.046	6.395LeuVal: 6.395 ± 0.059	1.1LeuTrp: 1.1 ± 0.025	3.253LeuTyr: 3.253 ± 0.037	0.0LeuXaa: 0.0 ± 0.0
Met	2.079MetAla: 2.079 ± 0.033	0.165MetCys: 0.165 ± 0.009	1.512MetAsp: 1.512 ± 0.031	1.793MetGlu: 1.793 ± 0.027	1.132MetPhe: 1.132 ± 0.023	1.789MetGly: 1.789 ± 0.029	0.529MetHis: 0.529 ± 0.016	2.058MetIle: 2.058 ± 0.033	2.206MetLys: 2.206 ± 0.031	2.91MetLeu: 2.91 ± 0.039	0.885MetMet: 0.885 ± 0.021	1.559MetAsn: 1.559 ± 0.028	1.159MetPro: 1.159 ± 0.022	1.076MetGln: 1.076 ± 0.021	1.251MetArg: 1.251 ± 0.022	1.828MetSer: 1.828 ± 0.03	1.704MetThr: 1.704 ± 0.03	1.833MetVal: 1.833 ± 0.033	0.259MetTrp: 0.259 ± 0.011	0.822MetTyr: 0.822 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.902AsnAla: 2.902 ± 0.038	0.266AsnCys: 0.266 ± 0.011	2.076AsnAsp: 2.076 ± 0.028	2.756AsnGlu: 2.756 ± 0.036	1.524AsnPhe: 1.524 ± 0.028	3.384AsnGly: 3.384 ± 0.046	0.926AsnHis: 0.926 ± 0.021	2.592AsnIle: 2.592 ± 0.035	2.486AsnLys: 2.486 ± 0.037	3.695AsnLeu: 3.695 ± 0.046	1.127AsnMet: 1.127 ± 0.024	1.826AsnAsn: 1.826 ± 0.035	2.15AsnPro: 2.15 ± 0.037	1.79AsnGln: 1.79 ± 0.035	2.053AsnArg: 2.053 ± 0.034	2.331AsnSer: 2.331 ± 0.036	2.152AsnThr: 2.152 ± 0.033	2.837AsnVal: 2.837 ± 0.038	0.622AsnTrp: 0.622 ± 0.017	1.552AsnTyr: 1.552 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	2.954ProAla: 2.954 ± 0.045	0.221ProCys: 0.221 ± 0.009	2.387ProAsp: 2.387 ± 0.035	2.942ProGlu: 2.942 ± 0.04	1.971ProPhe: 1.971 ± 0.033	2.662ProGly: 2.662 ± 0.04	0.927ProHis: 0.927 ± 0.019	2.913ProIle: 2.913 ± 0.035	1.985ProLys: 1.985 ± 0.031	4.015ProLeu: 4.015 ± 0.044	1.012ProMet: 1.012 ± 0.023	1.774ProAsn: 1.774 ± 0.031	1.162ProPro: 1.162 ± 0.026	1.424ProGln: 1.424 ± 0.026	1.289ProArg: 1.289 ± 0.024	2.739ProSer: 2.739 ± 0.037	2.29ProThr: 2.29 ± 0.043	3.047ProVal: 3.047 ± 0.04	0.542ProTrp: 0.542 ± 0.014	1.552ProTyr: 1.552 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.201GlnAla: 3.201 ± 0.04	0.212GlnCys: 0.212 ± 0.009	1.785GlnAsp: 1.785 ± 0.027	2.668GlnGlu: 2.668 ± 0.035	1.711GlnPhe: 1.711 ± 0.03	2.683GlnGly: 2.683 ± 0.035	0.839GlnHis: 0.839 ± 0.019	2.896GlnIle: 2.896 ± 0.037	2.252GlnLys: 2.252 ± 0.033	4.408GlnLeu: 4.408 ± 0.053	1.311GlnMet: 1.311 ± 0.022	1.543GlnAsn: 1.543 ± 0.031	1.489GlnPro: 1.489 ± 0.026	1.956GlnGln: 1.956 ± 0.043	1.72GlnArg: 1.72 ± 0.03	2.469GlnSer: 2.469 ± 0.033	2.231GlnThr: 2.231 ± 0.03	2.832GlnVal: 2.832 ± 0.037	0.55GlnTrp: 0.55 ± 0.017	1.331GlnTyr: 1.331 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	2.908ArgAla: 2.908 ± 0.038	0.347ArgCys: 0.347 ± 0.013	2.194ArgAsp: 2.194 ± 0.035	3.149ArgGlu: 3.149 ± 0.039	2.14ArgPhe: 2.14 ± 0.032	2.667ArgGly: 2.667 ± 0.035	0.904ArgHis: 0.904 ± 0.021	3.68ArgIle: 3.68 ± 0.04	2.942ArgLys: 2.942 ± 0.036	4.658ArgLeu: 4.658 ± 0.054	1.545ArgMet: 1.545 ± 0.027	2.008ArgAsn: 2.008 ± 0.031	1.524ArgPro: 1.524 ± 0.03	1.747ArgGln: 1.747 ± 0.031	2.179ArgArg: 2.179 ± 0.039	2.885ArgSer: 2.885 ± 0.04	2.435ArgThr: 2.435 ± 0.033	3.032ArgVal: 3.032 ± 0.043	0.649ArgTrp: 0.649 ± 0.017	1.685ArgTyr: 1.685 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	4.529SerAla: 4.529 ± 0.046	0.468SerCys: 0.468 ± 0.016	3.249SerAsp: 3.249 ± 0.038	3.833SerGlu: 3.833 ± 0.045	3.203SerPhe: 3.203 ± 0.036	5.153SerGly: 5.153 ± 0.057	1.288SerHis: 1.288 ± 0.024	4.753SerIle: 4.753 ± 0.048	3.656SerLys: 3.656 ± 0.044	6.696SerLeu: 6.696 ± 0.059	1.866SerMet: 1.866 ± 0.029	2.579SerAsn: 2.579 ± 0.035	2.494SerPro: 2.494 ± 0.03	2.248SerGln: 2.248 ± 0.027	2.754SerArg: 2.754 ± 0.035	4.75SerSer: 4.75 ± 0.055	3.544SerThr: 3.544 ± 0.043	4.537SerVal: 4.537 ± 0.046	0.89SerTrp: 0.89 ± 0.023	2.415SerTyr: 2.415 ± 0.037	0.001SerXaa: 0.001 ± 0.001
Thr	4.522ThrAla: 4.522 ± 0.06	0.378ThrCys: 0.378 ± 0.013	2.928ThrAsp: 2.928 ± 0.041	3.148ThrGlu: 3.148 ± 0.04	2.581ThrPhe: 2.581 ± 0.033	4.32ThrGly: 4.32 ± 0.045	1.079ThrHis: 1.079 ± 0.022	4.227ThrIle: 4.227 ± 0.048	2.88ThrLys: 2.88 ± 0.034	5.641ThrLeu: 5.641 ± 0.054	1.471ThrMet: 1.471 ± 0.024	2.403ThrAsn: 2.403 ± 0.037	2.601ThrPro: 2.601 ± 0.04	1.799ThrGln: 1.799 ± 0.025	2.063ThrArg: 2.063 ± 0.027	3.602ThrSer: 3.602 ± 0.04	3.244ThrThr: 3.244 ± 0.043	4.278ThrVal: 4.278 ± 0.047	0.73ThrTrp: 0.73 ± 0.019	2.157ThrTyr: 2.157 ± 0.038	0.001ThrXaa: 0.001 ± 0.001
Val	4.937ValAla: 4.937 ± 0.048	0.607ValCys: 0.607 ± 0.016	3.691ValAsp: 3.691 ± 0.047	4.168ValGlu: 4.168 ± 0.04	2.967ValPhe: 2.967 ± 0.041	4.75ValGly: 4.75 ± 0.052	1.523ValHis: 1.523 ± 0.026	5.204ValIle: 5.204 ± 0.052	4.189ValLys: 4.189 ± 0.05	6.861ValLeu: 6.861 ± 0.053	1.981ValMet: 1.981 ± 0.028	2.996ValAsn: 2.996 ± 0.039	2.98ValPro: 2.98 ± 0.036	2.651ValGln: 2.651 ± 0.035	3.169ValArg: 3.169 ± 0.04	4.859ValSer: 4.859 ± 0.05	4.464ValThr: 4.464 ± 0.055	4.962ValVal: 4.962 ± 0.05	0.854ValTrp: 0.854 ± 0.019	2.418ValTyr: 2.418 ± 0.036	0.001ValXaa: 0.001 ± 0.001
Trp	0.82TrpAla: 0.82 ± 0.02	0.125TrpCys: 0.125 ± 0.008	0.713TrpAsp: 0.713 ± 0.019	0.788TrpGlu: 0.788 ± 0.02	0.635TrpPhe: 0.635 ± 0.018	0.88TrpGly: 0.88 ± 0.022	0.329TrpHis: 0.329 ± 0.012	0.956TrpIle: 0.956 ± 0.022	0.87TrpLys: 0.87 ± 0.02	1.43TrpLeu: 1.43 ± 0.028	0.448TrpMet: 0.448 ± 0.015	0.76TrpAsn: 0.76 ± 0.018	0.398TrpPro: 0.398 ± 0.014	0.528TrpGln: 0.528 ± 0.014	0.586TrpArg: 0.586 ± 0.015	0.87TrpSer: 0.87 ± 0.02	0.7TrpThr: 0.7 ± 0.02	0.943TrpVal: 0.943 ± 0.021	0.201TrpTrp: 0.201 ± 0.011	0.475TrpTyr: 0.475 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.602TyrAla: 2.602 ± 0.036	0.282TyrCys: 0.282 ± 0.011	1.79TyrAsp: 1.79 ± 0.025	2.205TyrGlu: 2.205 ± 0.036	1.715TyrPhe: 1.715 ± 0.029	2.612TyrGly: 2.612 ± 0.036	0.805TyrHis: 0.805 ± 0.018	2.198TyrIle: 2.198 ± 0.032	1.832TyrLys: 1.832 ± 0.027	3.585TyrLeu: 3.585 ± 0.04	0.968TyrMet: 0.968 ± 0.022	1.502TyrAsn: 1.502 ± 0.03	1.563TyrPro: 1.563 ± 0.029	1.351TyrGln: 1.351 ± 0.025	1.923TyrArg: 1.923 ± 0.032	2.214TyrSer: 2.214 ± 0.03	1.955TyrThr: 1.955 ± 0.038	2.466TyrVal: 2.466 ± 0.037	0.526TyrTrp: 0.526 ± 0.018	1.364TyrTyr: 1.364 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6948 proteins (2269465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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