Amino acid dipepetide frequency for Bacillus sp. FJAT-27264

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.615AlaAla: 8.615 ± 0.118	0.617AlaCys: 0.617 ± 0.022	4.094AlaAsp: 4.094 ± 0.052	5.669AlaGlu: 5.669 ± 0.073	3.198AlaPhe: 3.198 ± 0.041	6.864AlaGly: 6.864 ± 0.082	1.288AlaHis: 1.288 ± 0.028	5.136AlaIle: 5.136 ± 0.062	4.119AlaLys: 4.119 ± 0.051	8.395AlaLeu: 8.395 ± 0.081	2.087AlaMet: 2.087 ± 0.039	2.617AlaAsn: 2.617 ± 0.045	2.897AlaPro: 2.897 ± 0.055	2.713AlaGln: 2.713 ± 0.048	3.359AlaArg: 3.359 ± 0.047	4.98AlaSer: 4.98 ± 0.06	3.954AlaThr: 3.954 ± 0.063	6.637AlaVal: 6.637 ± 0.068	0.889AlaTrp: 0.889 ± 0.024	2.554AlaTyr: 2.554 ± 0.039	0.0AlaXaa: 0.0 ± 0.0
Cys	0.457CysAla: 0.457 ± 0.018	0.105CysCys: 0.105 ± 0.007	0.367CysAsp: 0.367 ± 0.014	0.378CysGlu: 0.378 ± 0.015	0.305CysPhe: 0.305 ± 0.013	0.702CysGly: 0.702 ± 0.021	0.169CysHis: 0.169 ± 0.011	0.496CysIle: 0.496 ± 0.017	0.28CysLys: 0.28 ± 0.011	0.725CysLeu: 0.725 ± 0.021	0.194CysMet: 0.194 ± 0.011	0.257CysAsn: 0.257 ± 0.014	0.299CysPro: 0.299 ± 0.014	0.217CysGln: 0.217 ± 0.012	0.413CysArg: 0.413 ± 0.015	0.533CysSer: 0.533 ± 0.018	0.344CysThr: 0.344 ± 0.016	0.402CysVal: 0.402 ± 0.015	0.084CysTrp: 0.084 ± 0.006	0.248CysTyr: 0.248 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.636AspAla: 3.636 ± 0.053	0.347AspCys: 0.347 ± 0.015	2.269AspAsp: 2.269 ± 0.043	3.534AspGlu: 3.534 ± 0.046	2.217AspPhe: 2.217 ± 0.036	3.853AspGly: 3.853 ± 0.053	1.108AspHis: 1.108 ± 0.025	3.695AspIle: 3.695 ± 0.047	2.895AspLys: 2.895 ± 0.049	4.984AspLeu: 4.984 ± 0.056	1.278AspMet: 1.278 ± 0.025	2.052AspAsn: 2.052 ± 0.039	2.232AspPro: 2.232 ± 0.035	1.774AspGln: 1.774 ± 0.03	2.461AspArg: 2.461 ± 0.04	3.041AspSer: 3.041 ± 0.044	2.625AspThr: 2.625 ± 0.03	3.447AspVal: 3.447 ± 0.048	0.777AspTrp: 0.777 ± 0.021	2.134AspTyr: 2.134 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	5.746GluAla: 5.746 ± 0.064	0.362GluCys: 0.362 ± 0.015	3.221GluAsp: 3.221 ± 0.04	5.407GluGlu: 5.407 ± 0.072	2.239GluPhe: 2.239 ± 0.038	4.577GluGly: 4.577 ± 0.057	1.493GluHis: 1.493 ± 0.033	4.52GluIle: 4.52 ± 0.059	3.885GluLys: 3.885 ± 0.056	7.274GluLeu: 7.274 ± 0.076	1.947GluMet: 1.947 ± 0.037	2.559GluAsn: 2.559 ± 0.042	2.137GluPro: 2.137 ± 0.035	3.537GluGln: 3.537 ± 0.057	3.84GluArg: 3.84 ± 0.07	3.594GluSer: 3.594 ± 0.053	3.175GluThr: 3.175 ± 0.054	4.549GluVal: 4.549 ± 0.052	0.899GluTrp: 0.899 ± 0.024	2.042GluTyr: 2.042 ± 0.036	0.001GluXaa: 0.001 ± 0.001
Phe	3.13PheAla: 3.13 ± 0.041	0.352PheCys: 0.352 ± 0.014	2.141PheAsp: 2.141 ± 0.032	2.347PheGlu: 2.347 ± 0.04	1.839PhePhe: 1.839 ± 0.04	3.19PheGly: 3.19 ± 0.051	0.839PheHis: 0.839 ± 0.022	2.977PheIle: 2.977 ± 0.045	2.095PheLys: 2.095 ± 0.032	3.88PheLeu: 3.88 ± 0.06	1.125PheMet: 1.125 ± 0.031	1.749PheAsn: 1.749 ± 0.036	1.593PhePro: 1.593 ± 0.03	1.376PheGln: 1.376 ± 0.03	1.906PheArg: 1.906 ± 0.033	2.98PheSer: 2.98 ± 0.039	2.577PheThr: 2.577 ± 0.037	2.814PheVal: 2.814 ± 0.042	0.548PheTrp: 0.548 ± 0.016	1.472PheTyr: 1.472 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	5.54GlyAla: 5.54 ± 0.074	0.659GlyCys: 0.659 ± 0.023	3.669GlyAsp: 3.669 ± 0.049	4.592GlyGlu: 4.592 ± 0.052	3.195GlyPhe: 3.195 ± 0.042	5.751GlyGly: 5.751 ± 0.082	1.492GlyHis: 1.492 ± 0.033	5.794GlyIle: 5.794 ± 0.061	4.448GlyLys: 4.448 ± 0.056	7.647GlyLeu: 7.647 ± 0.082	2.303GlyMet: 2.303 ± 0.036	2.921GlyAsn: 2.921 ± 0.045	1.915GlyPro: 1.915 ± 0.037	2.812GlyGln: 2.812 ± 0.046	3.489GlyArg: 3.489 ± 0.051	5.103GlySer: 5.103 ± 0.071	4.638GlyThr: 4.638 ± 0.068	5.361GlyVal: 5.361 ± 0.059	1.046GlyTrp: 1.046 ± 0.027	2.984GlyTyr: 2.984 ± 0.04	0.002GlyXaa: 0.002 ± 0.001
His	1.385HisAla: 1.385 ± 0.033	0.183HisCys: 0.183 ± 0.009	0.973HisAsp: 0.973 ± 0.021	1.262HisGlu: 1.262 ± 0.025	1.015HisPhe: 1.015 ± 0.024	1.487HisGly: 1.487 ± 0.034	0.536HisHis: 0.536 ± 0.019	1.324HisIle: 1.324 ± 0.029	0.904HisLys: 0.904 ± 0.023	2.108HisLeu: 2.108 ± 0.041	0.548HisMet: 0.548 ± 0.016	0.74HisAsn: 0.74 ± 0.019	1.171HisPro: 1.171 ± 0.028	0.725HisGln: 0.725 ± 0.017	1.02HisArg: 1.02 ± 0.025	1.337HisSer: 1.337 ± 0.029	1.11HisThr: 1.11 ± 0.027	1.299HisVal: 1.299 ± 0.028	0.322HisTrp: 0.322 ± 0.015	0.858HisTyr: 0.858 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.994IleAla: 5.994 ± 0.053	0.557IleCys: 0.557 ± 0.019	3.397IleAsp: 3.397 ± 0.045	4.115IleGlu: 4.115 ± 0.054	2.493IlePhe: 2.493 ± 0.042	5.243IleGly: 5.243 ± 0.061	1.472IleHis: 1.472 ± 0.03	4.496IleIle: 4.496 ± 0.063	3.168IleLys: 3.168 ± 0.04	6.33IleLeu: 6.33 ± 0.081	1.644IleMet: 1.644 ± 0.037	2.571IleAsn: 2.571 ± 0.043	3.117IlePro: 3.117 ± 0.04	2.491IleGln: 2.491 ± 0.044	3.413IleArg: 3.413 ± 0.049	4.9IleSer: 4.9 ± 0.06	4.269IleThr: 4.269 ± 0.056	5.118IleVal: 5.118 ± 0.056	0.728IleTrp: 0.728 ± 0.02	2.111IleTyr: 2.111 ± 0.035	0.001IleXaa: 0.001 ± 0.001
Lys	4.207LysAla: 4.207 ± 0.057	0.22LysCys: 0.22 ± 0.011	3.119LysAsp: 3.119 ± 0.04	4.353LysGlu: 4.353 ± 0.06	1.611LysPhe: 1.611 ± 0.028	3.751LysGly: 3.751 ± 0.051	1.012LysHis: 1.012 ± 0.026	3.412LysIle: 3.412 ± 0.041	3.215LysLys: 3.215 ± 0.058	5.551LysLeu: 5.551 ± 0.058	1.562LysMet: 1.562 ± 0.03	2.256LysAsn: 2.256 ± 0.036	2.127LysPro: 2.127 ± 0.04	2.328LysGln: 2.328 ± 0.044	2.64LysArg: 2.64 ± 0.045	3.093LysSer: 3.093 ± 0.038	2.847LysThr: 2.847 ± 0.041	3.835LysVal: 3.835 ± 0.052	0.7LysTrp: 0.7 ± 0.024	1.846LysTyr: 1.846 ± 0.036	0.001LysXaa: 0.001 ± 0.001
Leu	8.298LeuAla: 8.298 ± 0.095	0.815LeuCys: 0.815 ± 0.025	5.119LeuAsp: 5.119 ± 0.057	6.55LeuGlu: 6.55 ± 0.076	4.503LeuPhe: 4.503 ± 0.072	7.246LeuGly: 7.246 ± 0.074	2.199LeuHis: 2.199 ± 0.045	6.654LeuIle: 6.654 ± 0.068	5.638LeuLys: 5.638 ± 0.064	11.85LeuLeu: 11.85 ± 0.13	2.675LeuMet: 2.675 ± 0.041	4.219LeuAsn: 4.219 ± 0.054	4.737LeuPro: 4.737 ± 0.052	4.197LeuGln: 4.197 ± 0.053	5.007LeuArg: 5.007 ± 0.06	7.711LeuSer: 7.711 ± 0.067	5.862LeuThr: 5.862 ± 0.066	6.47LeuVal: 6.47 ± 0.058	1.092LeuTrp: 1.092 ± 0.03	3.323LeuTyr: 3.323 ± 0.05	0.002LeuXaa: 0.002 ± 0.001
Met	2.041MetAla: 2.041 ± 0.036	0.162MetCys: 0.162 ± 0.009	1.454MetAsp: 1.454 ± 0.032	1.77MetGlu: 1.77 ± 0.032	0.998MetPhe: 0.998 ± 0.026	1.732MetGly: 1.732 ± 0.035	0.434MetHis: 0.434 ± 0.015	1.876MetIle: 1.876 ± 0.034	1.861MetLys: 1.861 ± 0.029	2.907MetLeu: 2.907 ± 0.045	0.812MetMet: 0.812 ± 0.023	1.487MetAsn: 1.487 ± 0.032	1.064MetPro: 1.064 ± 0.021	0.981MetGln: 0.981 ± 0.02	1.163MetArg: 1.163 ± 0.023	1.829MetSer: 1.829 ± 0.029	1.629MetThr: 1.629 ± 0.029	1.676MetVal: 1.676 ± 0.033	0.199MetTrp: 0.199 ± 0.012	0.739MetTyr: 0.739 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	2.885AsnAla: 2.885 ± 0.043	0.231AsnCys: 0.231 ± 0.011	1.997AsnAsp: 1.997 ± 0.034	2.492AsnGlu: 2.492 ± 0.044	1.436AsnPhe: 1.436 ± 0.033	3.272AsnGly: 3.272 ± 0.062	0.903AsnHis: 0.903 ± 0.027	2.693AsnIle: 2.693 ± 0.041	2.246AsnLys: 2.246 ± 0.04	3.715AsnLeu: 3.715 ± 0.053	1.016AsnMet: 1.016 ± 0.024	1.832AsnAsn: 1.832 ± 0.039	2.067AsnPro: 2.067 ± 0.037	1.453AsnGln: 1.453 ± 0.031	2.023AsnArg: 2.023 ± 0.032	2.407AsnSer: 2.407 ± 0.04	2.263AsnThr: 2.263 ± 0.04	2.743AsnVal: 2.743 ± 0.041	0.55AsnTrp: 0.55 ± 0.017	1.486AsnTyr: 1.486 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	3.437ProAla: 3.437 ± 0.049	0.209ProCys: 0.209 ± 0.012	2.435ProAsp: 2.435 ± 0.04	3.482ProGlu: 3.482 ± 0.051	1.823ProPhe: 1.823 ± 0.033	3.17ProGly: 3.17 ± 0.052	0.804ProHis: 0.804 ± 0.021	2.25ProIle: 2.25 ± 0.036	1.757ProLys: 1.757 ± 0.032	4.181ProLeu: 4.181 ± 0.051	0.886ProMet: 0.886 ± 0.024	1.412ProAsn: 1.412 ± 0.034	1.253ProPro: 1.253 ± 0.032	1.513ProGln: 1.513 ± 0.031	1.381ProArg: 1.381 ± 0.028	2.618ProSer: 2.618 ± 0.045	2.135ProThr: 2.135 ± 0.074	3.379ProVal: 3.379 ± 0.05	0.491ProTrp: 0.491 ± 0.018	1.501ProTyr: 1.501 ± 0.032	0.002ProXaa: 0.002 ± 0.001
Gln	3.199GlnAla: 3.199 ± 0.046	0.201GlnCys: 0.201 ± 0.011	1.722GlnAsp: 1.722 ± 0.033	2.776GlnGlu: 2.776 ± 0.049	1.443GlnPhe: 1.443 ± 0.028	2.765GlnGly: 2.765 ± 0.045	0.811GlnHis: 0.811 ± 0.022	2.432GlnIle: 2.432 ± 0.042	1.989GlnLys: 1.989 ± 0.04	4.213GlnLeu: 4.213 ± 0.052	1.092GlnMet: 1.092 ± 0.026	1.491GlnAsn: 1.491 ± 0.028	1.588GlnPro: 1.588 ± 0.033	1.889GlnGln: 1.889 ± 0.04	1.94GlnArg: 1.94 ± 0.035	2.382GlnSer: 2.382 ± 0.041	1.879GlnThr: 1.879 ± 0.036	2.472GlnVal: 2.472 ± 0.036	0.543GlnTrp: 0.543 ± 0.027	1.394GlnTyr: 1.394 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	3.122ArgAla: 3.122 ± 0.051	0.322ArgCys: 0.322 ± 0.014	2.372ArgAsp: 2.372 ± 0.037	3.668ArgGlu: 3.668 ± 0.052	2.014ArgPhe: 2.014 ± 0.035	2.969ArgGly: 2.969 ± 0.049	1.048ArgHis: 1.048 ± 0.026	3.409ArgIle: 3.409 ± 0.052	3.007ArgLys: 3.007 ± 0.042	5.123ArgLeu: 5.123 ± 0.07	1.48ArgMet: 1.48 ± 0.025	1.949ArgAsn: 1.949 ± 0.035	1.625ArgPro: 1.625 ± 0.028	2.02ArgGln: 2.02 ± 0.037	2.676ArgArg: 2.676 ± 0.043	3.211ArgSer: 3.211 ± 0.048	2.538ArgThr: 2.538 ± 0.037	2.967ArgVal: 2.967 ± 0.046	0.613ArgTrp: 0.613 ± 0.02	1.856ArgTyr: 1.856 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	5.112SerAla: 5.112 ± 0.065	0.406SerCys: 0.406 ± 0.014	3.132SerAsp: 3.132 ± 0.051	3.84SerGlu: 3.84 ± 0.054	3.075SerPhe: 3.075 ± 0.038	5.819SerGly: 5.819 ± 0.067	1.288SerHis: 1.288 ± 0.029	4.598SerIle: 4.598 ± 0.055	3.518SerLys: 3.518 ± 0.053	6.952SerLeu: 6.952 ± 0.075	1.732SerMet: 1.732 ± 0.029	2.582SerAsn: 2.582 ± 0.05	2.719SerPro: 2.719 ± 0.044	2.274SerGln: 2.274 ± 0.039	3.216SerArg: 3.216 ± 0.043	4.85SerSer: 4.85 ± 0.072	3.44SerThr: 3.44 ± 0.052	4.666SerVal: 4.666 ± 0.059	0.823SerTrp: 0.823 ± 0.023	2.37SerTyr: 2.37 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	5.072ThrAla: 5.072 ± 0.072	0.318ThrCys: 0.318 ± 0.016	2.769ThrAsp: 2.769 ± 0.042	3.418ThrGlu: 3.418 ± 0.065	2.409ThrPhe: 2.409 ± 0.039	4.81ThrGly: 4.81 ± 0.068	1.006ThrHis: 1.006 ± 0.02	3.688ThrIle: 3.688 ± 0.053	2.428ThrLys: 2.428 ± 0.036	5.961ThrLeu: 5.961 ± 0.061	1.22ThrMet: 1.22 ± 0.028	1.92ThrAsn: 1.92 ± 0.043	2.778ThrPro: 2.778 ± 0.067	1.624ThrGln: 1.624 ± 0.027	2.262ThrArg: 2.262 ± 0.036	3.595ThrSer: 3.595 ± 0.059	3.143ThrThr: 3.143 ± 0.06	4.708ThrVal: 4.708 ± 0.068	0.618ThrTrp: 0.618 ± 0.022	1.959ThrTyr: 1.959 ± 0.038	0.001ThrXaa: 0.001 ± 0.001
Val	5.394ValAla: 5.394 ± 0.061	0.552ValCys: 0.552 ± 0.018	3.569ValAsp: 3.569 ± 0.052	4.327ValGlu: 4.327 ± 0.05	2.963ValPhe: 2.963 ± 0.045	4.571ValGly: 4.571 ± 0.049	1.411ValHis: 1.411 ± 0.029	5.068ValIle: 5.068 ± 0.062	3.805ValLys: 3.805 ± 0.059	7.53ValLeu: 7.53 ± 0.073	1.943ValMet: 1.943 ± 0.034	2.915ValAsn: 2.915 ± 0.05	2.959ValPro: 2.959 ± 0.043	2.641ValGln: 2.641 ± 0.042	3.206ValArg: 3.206 ± 0.043	4.994ValSer: 4.994 ± 0.059	4.619ValThr: 4.619 ± 0.075	5.082ValVal: 5.082 ± 0.06	0.796ValTrp: 0.796 ± 0.023	2.284ValTyr: 2.284 ± 0.038	0.002ValXaa: 0.002 ± 0.001
Trp	0.798TrpAla: 0.798 ± 0.023	0.085TrpCys: 0.085 ± 0.006	0.639TrpAsp: 0.639 ± 0.022	0.774TrpGlu: 0.774 ± 0.024	0.53TrpPhe: 0.53 ± 0.016	0.901TrpGly: 0.901 ± 0.023	0.25TrpHis: 0.25 ± 0.013	0.912TrpIle: 0.912 ± 0.026	0.732TrpLys: 0.732 ± 0.02	1.387TrpLeu: 1.387 ± 0.032	0.407TrpMet: 0.407 ± 0.017	0.704TrpAsn: 0.704 ± 0.02	0.338TrpPro: 0.338 ± 0.014	0.452TrpGln: 0.452 ± 0.016	0.604TrpArg: 0.604 ± 0.018	0.839TrpSer: 0.839 ± 0.026	0.651TrpThr: 0.651 ± 0.02	0.757TrpVal: 0.757 ± 0.022	0.175TrpTrp: 0.175 ± 0.011	0.411TrpTyr: 0.411 ± 0.024	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.61TyrAla: 2.61 ± 0.04	0.268TyrCys: 0.268 ± 0.013	1.901TyrAsp: 1.901 ± 0.033	2.248TyrGlu: 2.248 ± 0.04	1.621TyrPhe: 1.621 ± 0.032	2.685TyrGly: 2.685 ± 0.039	0.742TyrHis: 0.742 ± 0.023	2.2TyrIle: 2.2 ± 0.033	1.697TyrLys: 1.697 ± 0.034	3.453TyrLeu: 3.453 ± 0.048	0.861TyrMet: 0.861 ± 0.024	1.479TyrAsn: 1.479 ± 0.031	1.505TyrPro: 1.505 ± 0.027	1.204TyrGln: 1.204 ± 0.028	1.969TyrArg: 1.969 ± 0.032	2.415TyrSer: 2.415 ± 0.037	2.009TyrThr: 2.009 ± 0.036	2.25TyrVal: 2.25 ± 0.032	0.453TyrTrp: 0.453 ± 0.018	1.371TyrTyr: 1.371 ± 0.032	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5758 proteins (1853569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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