Pseudomonas phage Ps59

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A4N5D4|A0A5A4N5D4_9CAUD DNA-binding domain-containing protein OS=Pseudomonas phage Ps59 OX=2301639 GN=Ps59_16 PE=4 SV=1
MM1 pKa = 7.8ADD3 pKa = 3.55VLEE6 pKa = 4.72IDD8 pKa = 5.11CPACSTPYY16 pKa = 10.43PEE18 pKa = 3.55ITARR22 pKa = 11.84SAAHH26 pKa = 6.75DD27 pKa = 4.06PSLIDD32 pKa = 5.49LVITCSNCGHH42 pKa = 7.22ILNAFVSLAEE52 pKa = 4.12MSVVPNPEE60 pKa = 4.01EE61 pKa = 4.23EE62 pKa = 4.42TSHH65 pKa = 6.27GG66 pKa = 3.71

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A4N1J9|A0A5A4N1J9_9CAUD Uncharacterized protein OS=Pseudomonas phage Ps59 OX=2301639 GN=Ps59_23 PE=4 SV=1
MM1 pKa = 6.88TEE3 pKa = 3.59KK4 pKa = 10.43RR5 pKa = 11.84VNEE8 pKa = 3.72TALRR12 pKa = 11.84VLRR15 pKa = 11.84VLIALKK21 pKa = 10.66GHH23 pKa = 5.93TLTGLSNGEE32 pKa = 3.8VAKK35 pKa = 10.98ALGEE39 pKa = 4.23SPANITRR46 pKa = 11.84YY47 pKa = 7.92MQTLIEE53 pKa = 4.78AGLAEE58 pKa = 4.15RR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 4.6DD62 pKa = 3.28GRR64 pKa = 11.84FAHH67 pKa = 6.2SVSMLQIAQAHH78 pKa = 6.56ADD80 pKa = 3.41HH81 pKa = 6.76VSRR84 pKa = 11.84MQNRR88 pKa = 11.84INEE91 pKa = 4.19LNRR94 pKa = 11.84RR95 pKa = 11.84VAAGSMII102 pKa = 3.79

Molecular weight: 11.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12627

47

1210

233.8

25.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.03 ± 0.71

0.839 ± 0.117

5.797 ± 0.232

6.391 ± 0.316

2.946 ± 0.206

7.682 ± 0.303

1.821 ± 0.153

4.158 ± 0.225

3.706 ± 0.322

10.303 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.122

3.112 ± 0.191

4.752 ± 0.303

5.053 ± 0.324

7.223 ± 0.322

5.726 ± 0.236

5.536 ± 0.274

6.644 ± 0.26

1.845 ± 0.164

2.368 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski