Pseudomonas phage DDSR119

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7ESZ0|A0A7D7ESZ0_9CAUD Uncharacterized protein OS=Pseudomonas phage DDSR119 OX=2759180 PE=4 SV=1
MM1 pKa = 7.65EE2 pKa = 4.81EE3 pKa = 4.37VNTGPLGLVPGVNCHH18 pKa = 5.46SFAFIGSQVVGGATEE33 pKa = 3.92TSDD36 pKa = 2.7IDD38 pKa = 3.26IAVRR42 pKa = 11.84IIEE45 pKa = 4.54GGDD48 pKa = 3.19PAEE51 pKa = 4.63LFNEE55 pKa = 4.28LLHH58 pKa = 6.68CGASEE63 pKa = 4.21VVISDD68 pKa = 3.7GEE70 pKa = 4.11GGYY73 pKa = 9.78EE74 pKa = 4.11VNDD77 pKa = 4.99DD78 pKa = 4.79GMTSCKK84 pKa = 10.34AWVGDD89 pKa = 4.07TVWNYY94 pKa = 11.16LIFTDD99 pKa = 4.24VEE101 pKa = 4.21YY102 pKa = 10.8FDD104 pKa = 5.6RR105 pKa = 11.84FVDD108 pKa = 3.23ATTIAKK114 pKa = 10.17RR115 pKa = 11.84FGVVDD120 pKa = 3.46KK121 pKa = 10.07PLRR124 pKa = 11.84VEE126 pKa = 4.98LFRR129 pKa = 11.84LLCDD133 pKa = 3.34GRR135 pKa = 11.84RR136 pKa = 11.84INGGYY141 pKa = 9.6FEE143 pKa = 5.54HH144 pKa = 7.26IKK146 pKa = 9.88IHH148 pKa = 5.37SAEE151 pKa = 4.18PEE153 pKa = 4.03NPFF156 pKa = 4.29

Molecular weight: 17.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7IG31|A0A7D7IG31_9CAUD Uncharacterized protein OS=Pseudomonas phage DDSR119 OX=2759180 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.01CRR4 pKa = 11.84KK5 pKa = 9.45SEE7 pKa = 4.01LVAEE11 pKa = 4.21ARR13 pKa = 11.84EE14 pKa = 4.06FHH16 pKa = 6.53KK17 pKa = 11.23LLMKK21 pKa = 10.33SIKK24 pKa = 9.91AGKK27 pKa = 9.5GEE29 pKa = 3.68HH30 pKa = 6.52SYY32 pKa = 10.9RR33 pKa = 11.84INRR36 pKa = 11.84DD37 pKa = 2.58RR38 pKa = 11.84KK39 pKa = 8.31MRR41 pKa = 11.84MARR44 pKa = 11.84VAKK47 pKa = 10.21RR48 pKa = 11.84DD49 pKa = 3.54MTEE52 pKa = 3.78WAAHH56 pKa = 5.59AAVSIRR62 pKa = 11.84PTALRR67 pKa = 11.84CEE69 pKa = 4.56CGAHH73 pKa = 5.17EE74 pKa = 4.59MLRR77 pKa = 11.84FVAVGATLHH86 pKa = 6.32LVSFF90 pKa = 5.01

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

16239

54

1182

213.7

23.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.721 ± 0.521

1.232 ± 0.111

6.213 ± 0.168

6.374 ± 0.284

3.171 ± 0.164

7.858 ± 0.254

2.008 ± 0.137

5.216 ± 0.161

5.468 ± 0.279

7.617 ± 0.226

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.931 ± 0.116

3.682 ± 0.221

4.502 ± 0.27

3.886 ± 0.229

6.663 ± 0.299

5.419 ± 0.22

5.678 ± 0.284

6.774 ± 0.194

1.552 ± 0.112

3.036 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski