Coniochaeta ligniaria NRRL 30616

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Coniochaetales; Coniochaetaceae; Coniochaeta; Coniochaeta ligniaria

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13658 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J7J191|A0A1J7J191_9PEZI Opsin-1 OS=Coniochaeta ligniaria NRRL 30616 OX=1408157 GN=CONLIGDRAFT_663288 PE=3 SV=1
MM1 pKa = 7.76SITGIDD7 pKa = 3.33ATGGTEE13 pKa = 4.06GKK15 pKa = 9.33SAKK18 pKa = 10.02GGHH21 pKa = 5.36QSMGDD26 pKa = 3.27SMDD29 pKa = 3.82AYY31 pKa = 10.15LVCHH35 pKa = 7.23DD36 pKa = 4.54CQGHH40 pKa = 6.56PSGGPDD46 pKa = 3.08GTGPDD51 pKa = 3.52GSFAVANDD59 pKa = 3.51AQDD62 pKa = 3.64YY63 pKa = 11.3AGDD66 pKa = 3.87EE67 pKa = 4.69VSWQWDD73 pKa = 3.64STNQRR78 pKa = 11.84YY79 pKa = 8.21FCVDD83 pKa = 2.86ANGNITWADD92 pKa = 3.56DD93 pKa = 3.55QGQSSSSAGQQ103 pKa = 2.98

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J7J4R6|A0A1J7J4R6_9PEZI Uncharacterized protein OS=Coniochaeta ligniaria NRRL 30616 OX=1408157 GN=CONLIGDRAFT_685764 PE=4 SV=1
MM1 pKa = 7.65KK2 pKa = 9.91IASTIKK8 pKa = 9.15IASIMRR14 pKa = 11.84IVNTIKK20 pKa = 10.25IASTTRR26 pKa = 11.84ITSTTRR32 pKa = 11.84IASITRR38 pKa = 11.84IASTTKK44 pKa = 7.91MTSTTRR50 pKa = 11.84MTSTTRR56 pKa = 11.84MMSTKK61 pKa = 10.25NSTMSTKK68 pKa = 10.66NSTTRR73 pKa = 11.84MMMTT77 pKa = 4.08

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13658

0

13658

6049712

49

4954

442.9

48.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.139 ± 0.018

1.326 ± 0.012

5.755 ± 0.016

5.916 ± 0.021

3.595 ± 0.012

7.251 ± 0.024

2.415 ± 0.01

4.482 ± 0.014

4.508 ± 0.018

8.875 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.146 ± 0.009

3.372 ± 0.012

6.248 ± 0.024

3.875 ± 0.02

6.336 ± 0.02

7.979 ± 0.023

6.108 ± 0.02

6.422 ± 0.016

1.526 ± 0.008

2.724 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski