Thermolongibacillus altinsuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Thermolongibacillus

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2961 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R1QET7|A0A4R1QET7_9BACI Uncharacterized protein OS=Thermolongibacillus altinsuensis OX=575256 GN=EDD69_10927 PE=4 SV=1
MM1 pKa = 7.42FDD3 pKa = 3.66DD4 pKa = 3.71WALALLTLVFYY15 pKa = 10.83IMYY18 pKa = 10.42NEE20 pKa = 4.39EE21 pKa = 3.91NDD23 pKa = 3.57FLDD26 pKa = 4.37FKK28 pKa = 10.66EE29 pKa = 3.94EE30 pKa = 3.77HH31 pKa = 6.03FFYY34 pKa = 11.39VNN36 pKa = 2.86

Molecular weight: 4.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4V2QAN7|A0A4V2QAN7_9BACI 3-dehydroquinate synthase OS=Thermolongibacillus altinsuensis OX=575256 GN=aroB PE=3 SV=1
MM1 pKa = 7.32QEE3 pKa = 4.04HH4 pKa = 7.02FYY6 pKa = 10.03FTTDD10 pKa = 2.97RR11 pKa = 11.84AKK13 pKa = 10.73LQKK16 pKa = 10.36QYY18 pKa = 11.27VAILFFVSGQLSLIQMYY35 pKa = 8.28LQRR38 pKa = 11.84RR39 pKa = 11.84NRR41 pKa = 11.84HH42 pKa = 5.22LLKK45 pKa = 10.67QEE47 pKa = 4.11DD48 pKa = 3.87KK49 pKa = 11.32VIIAVHH55 pKa = 5.14VLGKK59 pKa = 10.75LFGFTSEE66 pKa = 4.45RR67 pKa = 11.84AWHH70 pKa = 6.22RR71 pKa = 11.84FVIGNLFSKK80 pKa = 10.73DD81 pKa = 3.59LFPEE85 pKa = 4.19RR86 pKa = 11.84SRR88 pKa = 11.84YY89 pKa = 8.11NRR91 pKa = 11.84RR92 pKa = 11.84CRR94 pKa = 11.84NLGFAVKK101 pKa = 9.86WIRR104 pKa = 11.84YY105 pKa = 7.01QLAKK109 pKa = 10.31RR110 pKa = 11.84GQHH113 pKa = 5.36HH114 pKa = 7.46AYY116 pKa = 10.19AVVDD120 pKa = 3.74SLPIEE125 pKa = 4.61LCHH128 pKa = 6.3HH129 pKa = 5.83ARR131 pKa = 11.84MYY133 pKa = 9.82RR134 pKa = 11.84VKK136 pKa = 10.13RR137 pKa = 11.84FQGITDD143 pKa = 3.52IGYY146 pKa = 9.08CALKK150 pKa = 10.29RR151 pKa = 11.84WLTMGG156 pKa = 3.91

Molecular weight: 18.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2961

0

2961

844262

28

1490

285.1

32.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.343 ± 0.043

0.8 ± 0.015

4.637 ± 0.033

7.888 ± 0.056

4.546 ± 0.039

6.579 ± 0.044

2.313 ± 0.021

7.756 ± 0.043

7.04 ± 0.042

9.855 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.786 ± 0.018

3.891 ± 0.032

3.728 ± 0.03

3.979 ± 0.036

4.653 ± 0.038

5.189 ± 0.031

5.159 ± 0.026

7.248 ± 0.04

1.057 ± 0.018

3.552 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski