unidentified eubacterium SCB49

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; environmental samples

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6ETP2|A6ETP2_9BACT 3-dehydroquinate dehydratase OS=unidentified eubacterium SCB49 OX=50743 GN=aroQ PE=3 SV=1
MM1 pKa = 7.37KK2 pKa = 10.25KK3 pKa = 9.96IYY5 pKa = 10.17LLLGLCPILSVQSQNFEE22 pKa = 4.09EE23 pKa = 5.17VEE25 pKa = 4.03TDD27 pKa = 3.15MKK29 pKa = 10.92DD30 pKa = 3.44YY31 pKa = 10.79YY32 pKa = 11.11YY33 pKa = 11.14SSSNVADD40 pKa = 3.92FDD42 pKa = 4.47GDD44 pKa = 3.94GFVDD48 pKa = 3.51IVFNGAIDD56 pKa = 3.65SDD58 pKa = 4.31NNGEE62 pKa = 3.92VDD64 pKa = 3.42TTFNEE69 pKa = 4.71VYY71 pKa = 10.59KK72 pKa = 11.04NDD74 pKa = 4.01DD75 pKa = 3.23ATFTLFDD82 pKa = 4.74DD83 pKa = 4.45LGLYY87 pKa = 7.63ATHH90 pKa = 7.11LGDD93 pKa = 5.26IKK95 pKa = 11.08FLDD98 pKa = 3.98FDD100 pKa = 4.29NDD102 pKa = 3.49GLLDD106 pKa = 3.67IVSTGLSYY114 pKa = 11.72NDD116 pKa = 2.86ITDD119 pKa = 3.59YY120 pKa = 10.35QQYY123 pKa = 10.27RR124 pKa = 11.84LKK126 pKa = 10.2NTGTSLEE133 pKa = 4.19LEE135 pKa = 4.31EE136 pKa = 5.26NISGKK141 pKa = 9.89IFGSLEE147 pKa = 4.01VFDD150 pKa = 5.28FNHH153 pKa = 7.09DD154 pKa = 3.75GFVDD158 pKa = 3.73YY159 pKa = 10.89ALNGIQYY166 pKa = 9.77IEE168 pKa = 4.27EE169 pKa = 4.24DD170 pKa = 3.47QAFSFDD176 pKa = 4.26LDD178 pKa = 4.47FYY180 pKa = 11.33QNNGDD185 pKa = 4.03GFDD188 pKa = 3.76VTPAWLSGTQSGAFKK203 pKa = 11.06VLDD206 pKa = 4.22LNNDD210 pKa = 3.23NLLDD214 pKa = 3.88LVISGFNIDD223 pKa = 3.93YY224 pKa = 10.76EE225 pKa = 4.75PIFKK229 pKa = 10.61VYY231 pKa = 10.83LNNNGVLEE239 pKa = 4.43FSQDD243 pKa = 3.42LAPISDD249 pKa = 3.9GEE251 pKa = 4.05IAYY254 pKa = 9.23TDD256 pKa = 4.19FNADD260 pKa = 3.13GFLDD264 pKa = 3.93LAVVGVDD271 pKa = 3.82LDD273 pKa = 4.07YY274 pKa = 11.9NEE276 pKa = 4.28YY277 pKa = 10.68LAVLINDD284 pKa = 3.55GTGNFTTNVIDD295 pKa = 3.74NEE297 pKa = 4.53GVSGSSIDD305 pKa = 4.88VGDD308 pKa = 4.45LNNDD312 pKa = 3.22GYY314 pKa = 11.7YY315 pKa = 10.95DD316 pKa = 4.52FIIIGNDD323 pKa = 2.68ADD325 pKa = 3.86YY326 pKa = 11.29NGWVKK331 pKa = 10.49VFLYY335 pKa = 10.91DD336 pKa = 3.91NNTEE340 pKa = 4.32SFTKK344 pKa = 10.67SADD347 pKa = 3.18TGLYY351 pKa = 10.41NLGSNGDD358 pKa = 3.6INLFDD363 pKa = 4.37YY364 pKa = 11.59NNDD367 pKa = 2.97NHH369 pKa = 7.86LDD371 pKa = 3.56VMMSGFDD378 pKa = 3.28WADD381 pKa = 2.84SDD383 pKa = 5.06FPSLTKK389 pKa = 10.86LFTNLSTEE397 pKa = 4.42EE398 pKa = 4.06NQKK401 pKa = 10.03PLPPTEE407 pKa = 5.28LDD409 pKa = 3.4LTQDD413 pKa = 3.48DD414 pKa = 4.07NKK416 pKa = 10.25YY417 pKa = 7.91TFTWSGASDD426 pKa = 4.02DD427 pKa = 4.03KK428 pKa = 11.52TPTNALQYY436 pKa = 8.68EE437 pKa = 4.6IKK439 pKa = 10.89VGTTSGAQDD448 pKa = 3.24VAKK451 pKa = 10.76YY452 pKa = 10.25IVTTPSWFLEE462 pKa = 4.21LEE464 pKa = 4.11NMPEE468 pKa = 3.67NLYY471 pKa = 10.36WSVKK475 pKa = 10.62SIDD478 pKa = 3.35ASKK481 pKa = 10.57ILSNSSDD488 pKa = 3.6EE489 pKa = 4.29QQLSVSDD496 pKa = 4.75FSLLNNIKK504 pKa = 10.23VYY506 pKa = 10.59PNPASGKK513 pKa = 8.56VFVSAEE519 pKa = 3.74GLIAVEE525 pKa = 4.76MYY527 pKa = 10.37DD528 pKa = 3.3IQGRR532 pKa = 11.84KK533 pKa = 9.45INVKK537 pKa = 10.25LNSDD541 pKa = 4.42FSLDD545 pKa = 3.31ISGLSSGVYY554 pKa = 7.92VLKK557 pKa = 10.79MNVDD561 pKa = 3.55GVLVSRR567 pKa = 11.84KK568 pKa = 10.06LSVNN572 pKa = 3.27

Molecular weight: 63.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6ESE5|A6ESE5_9BACT N-acetyltransferase domain-containing protein OS=unidentified eubacterium SCB49 OX=50743 GN=SCB49_00205 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.17RR22 pKa = 11.84MASVSGRR29 pKa = 11.84KK30 pKa = 9.02VIKK33 pKa = 10.15ARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.71GRR40 pKa = 11.84KK41 pKa = 8.56KK42 pKa = 10.24ISVSSEE48 pKa = 3.49FRR50 pKa = 11.84HH51 pKa = 6.21KK52 pKa = 10.57KK53 pKa = 9.45

Molecular weight: 6.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2926

0

2926

950377

21

2545

324.8

36.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.794 ± 0.048

0.765 ± 0.018

5.85 ± 0.054

6.662 ± 0.043

5.125 ± 0.034

6.438 ± 0.049

1.698 ± 0.019

7.854 ± 0.042

7.58 ± 0.064

9.173 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.169 ± 0.021

5.981 ± 0.05

3.325 ± 0.028

3.323 ± 0.023

3.269 ± 0.034

6.372 ± 0.034

6.254 ± 0.046

6.427 ± 0.036

0.973 ± 0.016

3.967 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski