Amino acid dipepetide frequency for Mycobacterium phage Sparky

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.07AlaAla: 16.07 ± 1.287
1.148AlaCys: 1.148 ± 0.269
7.836AlaAsp: 7.836 ± 0.576
7.885AlaGlu: 7.885 ± 0.843
2.396AlaPhe: 2.396 ± 0.3
7.386AlaGly: 7.386 ± 0.832
2.945AlaHis: 2.945 ± 0.464
5.19AlaIle: 5.19 ± 0.484
3.893AlaLys: 3.893 ± 0.485
9.183AlaLeu: 9.183 ± 0.811
2.745AlaMet: 2.745 ± 0.391
3.543AlaAsn: 3.543 ± 0.396
4.691AlaPro: 4.691 ± 0.536
5.29AlaGln: 5.29 ± 0.833
8.285AlaArg: 8.285 ± 0.782
4.691AlaSer: 4.691 ± 0.508
6.488AlaThr: 6.488 ± 0.619
7.436AlaVal: 7.436 ± 0.647
2.096AlaTrp: 2.096 ± 0.345
2.346AlaTyr: 2.346 ± 0.341
0.0AlaXaa: 0.0 ± 0.0
Cys
1.148CysAla: 1.148 ± 0.311
0.2CysCys: 0.2 ± 0.129
1.048CysAsp: 1.048 ± 0.27
0.799CysGlu: 0.799 ± 0.222
0.2CysPhe: 0.2 ± 0.092
1.447CysGly: 1.447 ± 0.28
0.399CysHis: 0.399 ± 0.143
0.349CysIle: 0.349 ± 0.128
0.399CysLys: 0.399 ± 0.149
0.699CysLeu: 0.699 ± 0.229
0.2CysMet: 0.2 ± 0.089
0.25CysAsn: 0.25 ± 0.098
1.248CysPro: 1.248 ± 0.263
0.449CysGln: 0.449 ± 0.15
0.948CysArg: 0.948 ± 0.276
0.599CysSer: 0.599 ± 0.154
0.699CysThr: 0.699 ± 0.194
0.549CysVal: 0.549 ± 0.166
0.15CysTrp: 0.15 ± 0.087
0.299CysTyr: 0.299 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
6.787AspAla: 6.787 ± 0.561
1.048AspCys: 1.048 ± 0.222
4.891AspAsp: 4.891 ± 0.577
4.841AspGlu: 4.841 ± 0.552
1.946AspPhe: 1.946 ± 0.28
6.238AspGly: 6.238 ± 0.694
1.896AspHis: 1.896 ± 0.32
3.394AspIle: 3.394 ± 0.424
1.697AspLys: 1.697 ± 0.287
5.14AspLeu: 5.14 ± 0.484
1.497AspMet: 1.497 ± 0.283
2.046AspAsn: 2.046 ± 0.329
5.14AspPro: 5.14 ± 0.575
2.346AspGln: 2.346 ± 0.396
4.192AspArg: 4.192 ± 0.429
3.194AspSer: 3.194 ± 0.393
2.945AspThr: 2.945 ± 0.397
4.691AspVal: 4.691 ± 0.586
1.747AspTrp: 1.747 ± 0.242
1.946AspTyr: 1.946 ± 0.383
0.0AspXaa: 0.0 ± 0.0
Glu
6.288GluAla: 6.288 ± 0.616
0.898GluCys: 0.898 ± 0.248
3.094GluAsp: 3.094 ± 0.416
2.945GluGlu: 2.945 ± 0.444
2.396GluPhe: 2.396 ± 0.324
3.044GluGly: 3.044 ± 0.5
1.797GluHis: 1.797 ± 0.308
2.994GluIle: 2.994 ± 0.386
1.597GluLys: 1.597 ± 0.324
4.941GluLeu: 4.941 ± 0.647
1.397GluMet: 1.397 ± 0.27
1.896GluAsn: 1.896 ± 0.31
3.294GluPro: 3.294 ± 0.502
2.945GluGln: 2.945 ± 0.381
5.24GluArg: 5.24 ± 0.627
3.593GluSer: 3.593 ± 0.308
3.394GluThr: 3.394 ± 0.403
3.643GluVal: 3.643 ± 0.412
1.697GluTrp: 1.697 ± 0.289
1.747GluTyr: 1.747 ± 0.305
0.0GluXaa: 0.0 ± 0.0
Phe
2.495PheAla: 2.495 ± 0.282
0.399PheCys: 0.399 ± 0.124
2.945PheAsp: 2.945 ± 0.47
1.547PheGlu: 1.547 ± 0.304
0.749PhePhe: 0.749 ± 0.179
3.094PheGly: 3.094 ± 0.357
0.749PheHis: 0.749 ± 0.235
1.298PheIle: 1.298 ± 0.255
0.749PheLys: 0.749 ± 0.182
2.296PheLeu: 2.296 ± 0.401
0.649PheMet: 0.649 ± 0.153
0.699PheAsn: 0.699 ± 0.179
1.597PhePro: 1.597 ± 0.306
0.998PheGln: 0.998 ± 0.178
1.547PheArg: 1.547 ± 0.227
1.048PheSer: 1.048 ± 0.29
1.847PheThr: 1.847 ± 0.296
2.396PheVal: 2.396 ± 0.335
0.599PheTrp: 0.599 ± 0.157
0.699PheTyr: 0.699 ± 0.236
0.0PheXaa: 0.0 ± 0.0
Gly
9.183GlyAla: 9.183 ± 1.054
0.948GlyCys: 0.948 ± 0.254
5.24GlyAsp: 5.24 ± 0.571
4.592GlyGlu: 4.592 ± 0.55
2.695GlyPhe: 2.695 ± 0.447
10.031GlyGly: 10.031 ± 1.724
1.896GlyHis: 1.896 ± 0.299
3.344GlyIle: 3.344 ± 0.394
3.044GlyLys: 3.044 ± 0.42
5.839GlyLeu: 5.839 ± 0.563
1.647GlyMet: 1.647 ± 0.324
2.945GlyAsn: 2.945 ± 0.549
3.743GlyPro: 3.743 ± 0.501
3.044GlyGln: 3.044 ± 0.527
6.089GlyArg: 6.089 ± 0.554
4.442GlySer: 4.442 ± 0.57
5.29GlyThr: 5.29 ± 0.696
5.839GlyVal: 5.839 ± 0.538
1.697GlyTrp: 1.697 ± 0.265
2.545GlyTyr: 2.545 ± 0.453
0.0GlyXaa: 0.0 ± 0.0
His
2.695HisAla: 2.695 ± 0.475
0.299HisCys: 0.299 ± 0.107
1.697HisAsp: 1.697 ± 0.32
1.547HisGlu: 1.547 ± 0.246
0.599HisPhe: 0.599 ± 0.152
2.046HisGly: 2.046 ± 0.362
0.948HisHis: 0.948 ± 0.24
0.948HisIle: 0.948 ± 0.204
0.998HisLys: 0.998 ± 0.321
1.547HisLeu: 1.547 ± 0.274
0.449HisMet: 0.449 ± 0.151
0.749HisAsn: 0.749 ± 0.153
2.445HisPro: 2.445 ± 0.374
0.898HisGln: 0.898 ± 0.221
2.595HisArg: 2.595 ± 0.446
1.048HisSer: 1.048 ± 0.289
1.547HisThr: 1.547 ± 0.326
1.597HisVal: 1.597 ± 0.261
0.699HisTrp: 0.699 ± 0.226
0.649HisTyr: 0.649 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
4.841IleAla: 4.841 ± 0.638
0.499IleCys: 0.499 ± 0.174
2.994IleAsp: 2.994 ± 0.362
3.194IleGlu: 3.194 ± 0.361
1.248IlePhe: 1.248 ± 0.325
4.043IleGly: 4.043 ± 0.441
1.148IleHis: 1.148 ± 0.264
1.747IleIle: 1.747 ± 0.312
0.599IleLys: 0.599 ± 0.159
2.096IleLeu: 2.096 ± 0.257
0.649IleMet: 0.649 ± 0.199
1.996IleAsn: 1.996 ± 0.258
2.994IlePro: 2.994 ± 0.403
2.046IleGln: 2.046 ± 0.295
2.945IleArg: 2.945 ± 0.366
2.495IleSer: 2.495 ± 0.432
3.294IleThr: 3.294 ± 0.375
4.592IleVal: 4.592 ± 0.509
0.599IleTrp: 0.599 ± 0.169
1.098IleTyr: 1.098 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
3.793LysAla: 3.793 ± 0.497
0.399LysCys: 0.399 ± 0.117
1.697LysAsp: 1.697 ± 0.338
1.298LysGlu: 1.298 ± 0.29
0.848LysPhe: 0.848 ± 0.201
2.096LysGly: 2.096 ± 0.319
0.799LysHis: 0.799 ± 0.195
1.447LysIle: 1.447 ± 0.298
0.799LysLys: 0.799 ± 0.203
3.094LysLeu: 3.094 ± 0.358
0.399LysMet: 0.399 ± 0.122
1.098LysAsn: 1.098 ± 0.309
2.695LysPro: 2.695 ± 0.451
0.998LysGln: 0.998 ± 0.182
2.445LysArg: 2.445 ± 0.367
1.896LysSer: 1.896 ± 0.333
2.196LysThr: 2.196 ± 0.299
1.647LysVal: 1.647 ± 0.27
0.998LysTrp: 0.998 ± 0.181
1.198LysTyr: 1.198 ± 0.191
0.0LysXaa: 0.0 ± 0.0
Leu
10.381LeuAla: 10.381 ± 0.861
0.649LeuCys: 0.649 ± 0.205
4.292LeuAsp: 4.292 ± 0.549
3.793LeuGlu: 3.793 ± 0.507
2.545LeuPhe: 2.545 ± 0.412
6.189LeuGly: 6.189 ± 0.66
1.447LeuHis: 1.447 ± 0.259
3.693LeuIle: 3.693 ± 0.487
1.847LeuLys: 1.847 ± 0.334
5.689LeuLeu: 5.689 ± 0.51
1.597LeuMet: 1.597 ± 0.292
3.144LeuAsn: 3.144 ± 0.598
4.292LeuPro: 4.292 ± 0.509
2.695LeuGln: 2.695 ± 0.337
4.592LeuArg: 4.592 ± 0.534
4.492LeuSer: 4.492 ± 0.519
4.991LeuThr: 4.991 ± 0.417
5.091LeuVal: 5.091 ± 0.441
1.298LeuTrp: 1.298 ± 0.219
1.847LeuTyr: 1.847 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
2.246MetAla: 2.246 ± 0.294
0.25MetCys: 0.25 ± 0.131
1.298MetAsp: 1.298 ± 0.235
0.649MetGlu: 0.649 ± 0.173
0.599MetPhe: 0.599 ± 0.168
1.497MetGly: 1.497 ± 0.273
0.499MetHis: 0.499 ± 0.141
0.898MetIle: 0.898 ± 0.2
0.599MetLys: 0.599 ± 0.175
1.547MetLeu: 1.547 ± 0.309
0.15MetMet: 0.15 ± 0.092
0.699MetAsn: 0.699 ± 0.205
1.747MetPro: 1.747 ± 0.297
0.599MetGln: 0.599 ± 0.154
1.248MetArg: 1.248 ± 0.215
1.946MetSer: 1.946 ± 0.284
1.797MetThr: 1.797 ± 0.308
1.198MetVal: 1.198 ± 0.199
0.549MetTrp: 0.549 ± 0.183
0.449MetTyr: 0.449 ± 0.139
0.0MetXaa: 0.0 ± 0.0
Asn
3.743AsnAla: 3.743 ± 0.44
0.449AsnCys: 0.449 ± 0.155
2.246AsnAsp: 2.246 ± 0.358
1.447AsnGlu: 1.447 ± 0.251
0.499AsnPhe: 0.499 ± 0.161
2.895AsnGly: 2.895 ± 0.347
1.348AsnHis: 1.348 ± 0.3
1.348AsnIle: 1.348 ± 0.308
1.198AsnLys: 1.198 ± 0.235
1.996AsnLeu: 1.996 ± 0.37
0.399AsnMet: 0.399 ± 0.125
1.547AsnAsn: 1.547 ± 0.3
3.194AsnPro: 3.194 ± 0.45
1.647AsnGln: 1.647 ± 0.341
2.246AsnArg: 2.246 ± 0.337
1.896AsnSer: 1.896 ± 0.343
1.797AsnThr: 1.797 ± 0.314
2.545AsnVal: 2.545 ± 0.233
0.649AsnTrp: 0.649 ± 0.168
0.749AsnTyr: 0.749 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
7.187ProAla: 7.187 ± 0.68
0.599ProCys: 0.599 ± 0.185
5.49ProAsp: 5.49 ± 0.505
4.542ProGlu: 4.542 ± 0.4
1.697ProPhe: 1.697 ± 0.293
5.49ProGly: 5.49 ± 0.538
1.597ProHis: 1.597 ± 0.262
3.444ProIle: 3.444 ± 0.451
1.847ProLys: 1.847 ± 0.34
4.092ProLeu: 4.092 ± 0.347
1.098ProMet: 1.098 ± 0.22
1.946ProAsn: 1.946 ± 0.29
3.843ProPro: 3.843 ± 0.401
2.595ProGln: 2.595 ± 0.406
4.192ProArg: 4.192 ± 0.554
2.845ProSer: 2.845 ± 0.352
3.294ProThr: 3.294 ± 0.381
3.993ProVal: 3.993 ± 0.478
1.348ProTrp: 1.348 ± 0.344
0.948ProTyr: 0.948 ± 0.175
0.0ProXaa: 0.0 ± 0.0
Gln
4.791GlnAla: 4.791 ± 0.751
0.399GlnCys: 0.399 ± 0.142
1.148GlnAsp: 1.148 ± 0.204
1.397GlnGlu: 1.397 ± 0.296
1.797GlnPhe: 1.797 ± 0.351
2.146GlnGly: 2.146 ± 0.369
0.848GlnHis: 0.848 ± 0.233
1.148GlnIle: 1.148 ± 0.188
1.298GlnLys: 1.298 ± 0.29
4.791GlnLeu: 4.791 ± 0.521
1.098GlnMet: 1.098 ± 0.178
1.597GlnAsn: 1.597 ± 0.294
2.445GlnPro: 2.445 ± 0.362
2.396GlnGln: 2.396 ± 0.482
4.142GlnArg: 4.142 ± 0.478
1.797GlnSer: 1.797 ± 0.404
2.096GlnThr: 2.096 ± 0.352
2.695GlnVal: 2.695 ± 0.372
1.048GlnTrp: 1.048 ± 0.188
0.599GlnTyr: 0.599 ± 0.158
0.0GlnXaa: 0.0 ± 0.0
Arg
7.686ArgAla: 7.686 ± 0.704
0.848ArgCys: 0.848 ± 0.234
5.59ArgAsp: 5.59 ± 0.469
4.492ArgGlu: 4.492 ± 0.446
2.046ArgPhe: 2.046 ± 0.337
5.689ArgGly: 5.689 ± 0.381
1.647ArgHis: 1.647 ± 0.332
3.444ArgIle: 3.444 ± 0.441
2.695ArgLys: 2.695 ± 0.337
5.24ArgLeu: 5.24 ± 0.749
1.996ArgMet: 1.996 ± 0.359
2.296ArgAsn: 2.296 ± 0.352
4.043ArgPro: 4.043 ± 0.547
3.494ArgGln: 3.494 ± 0.496
6.887ArgArg: 6.887 ± 0.789
3.893ArgSer: 3.893 ± 0.463
4.242ArgThr: 4.242 ± 0.556
4.342ArgVal: 4.342 ± 0.508
1.647ArgTrp: 1.647 ± 0.264
1.946ArgTyr: 1.946 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
4.592SerAla: 4.592 ± 0.578
0.449SerCys: 0.449 ± 0.186
3.543SerAsp: 3.543 ± 0.375
2.945SerGlu: 2.945 ± 0.438
1.547SerPhe: 1.547 ± 0.26
5.689SerGly: 5.689 ± 0.701
1.148SerHis: 1.148 ± 0.23
2.096SerIle: 2.096 ± 0.31
2.296SerLys: 2.296 ± 0.334
3.893SerLeu: 3.893 ± 0.45
1.447SerMet: 1.447 ± 0.229
1.747SerAsn: 1.747 ± 0.251
3.643SerPro: 3.643 ± 0.386
1.497SerGln: 1.497 ± 0.206
3.194SerArg: 3.194 ± 0.344
2.795SerSer: 2.795 ± 0.473
3.643SerThr: 3.643 ± 0.46
3.194SerVal: 3.194 ± 0.327
0.948SerTrp: 0.948 ± 0.205
1.148SerTyr: 1.148 ± 0.272
0.0SerXaa: 0.0 ± 0.0
Thr
7.037ThrAla: 7.037 ± 0.758
0.749ThrCys: 0.749 ± 0.202
4.641ThrAsp: 4.641 ± 0.457
3.593ThrGlu: 3.593 ± 0.366
1.647ThrPhe: 1.647 ± 0.275
5.989ThrGly: 5.989 ± 0.498
1.298ThrHis: 1.298 ± 0.237
3.144ThrIle: 3.144 ± 0.441
2.296ThrLys: 2.296 ± 0.331
4.092ThrLeu: 4.092 ± 0.477
0.799ThrMet: 0.799 ± 0.27
2.046ThrAsn: 2.046 ± 0.316
4.791ThrPro: 4.791 ± 0.477
1.248ThrGln: 1.248 ± 0.275
4.242ThrArg: 4.242 ± 0.457
2.994ThrSer: 2.994 ± 0.43
3.943ThrThr: 3.943 ± 0.466
5.14ThrVal: 5.14 ± 0.477
1.797ThrTrp: 1.797 ± 0.317
1.647ThrTyr: 1.647 ± 0.299
0.0ThrXaa: 0.0 ± 0.0
Val
5.54ValAla: 5.54 ± 0.662
1.098ValCys: 1.098 ± 0.257
4.991ValAsp: 4.991 ± 0.494
4.941ValGlu: 4.941 ± 0.457
1.298ValPhe: 1.298 ± 0.251
5.54ValGly: 5.54 ± 0.518
2.096ValHis: 2.096 ± 0.33
3.394ValIle: 3.394 ± 0.473
2.046ValLys: 2.046 ± 0.28
4.741ValLeu: 4.741 ± 0.454
1.248ValMet: 1.248 ± 0.246
2.296ValAsn: 2.296 ± 0.407
4.342ValPro: 4.342 ± 0.438
2.595ValGln: 2.595 ± 0.438
5.19ValArg: 5.19 ± 0.597
3.344ValSer: 3.344 ± 0.401
5.889ValThr: 5.889 ± 0.5
4.691ValVal: 4.691 ± 0.481
1.198ValTrp: 1.198 ± 0.304
1.946ValTyr: 1.946 ± 0.286
0.0ValXaa: 0.0 ± 0.0
Trp
2.196TrpAla: 2.196 ± 0.348
0.499TrpCys: 0.499 ± 0.149
1.397TrpAsp: 1.397 ± 0.317
0.998TrpGlu: 0.998 ± 0.244
0.799TrpPhe: 0.799 ± 0.227
1.547TrpGly: 1.547 ± 0.232
0.998TrpHis: 0.998 ± 0.244
1.098TrpIle: 1.098 ± 0.219
0.898TrpLys: 0.898 ± 0.218
1.447TrpLeu: 1.447 ± 0.264
0.499TrpMet: 0.499 ± 0.17
0.549TrpAsn: 0.549 ± 0.157
0.848TrpPro: 0.848 ± 0.195
0.998TrpGln: 0.998 ± 0.151
1.946TrpArg: 1.946 ± 0.276
1.148TrpSer: 1.148 ± 0.279
1.697TrpThr: 1.697 ± 0.27
1.048TrpVal: 1.048 ± 0.208
0.599TrpTrp: 0.599 ± 0.168
0.549TrpTyr: 0.549 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.845TyrAla: 2.845 ± 0.431
0.25TyrCys: 0.25 ± 0.12
1.547TyrAsp: 1.547 ± 0.284
1.397TyrGlu: 1.397 ± 0.228
0.848TyrPhe: 0.848 ± 0.192
1.996TyrGly: 1.996 ± 0.331
0.499TyrHis: 0.499 ± 0.184
0.699TyrIle: 0.699 ± 0.168
1.048TyrLys: 1.048 ± 0.186
2.196TyrLeu: 2.196 ± 0.327
0.449TyrMet: 0.449 ± 0.226
0.749TyrAsn: 0.749 ± 0.17
1.048TyrPro: 1.048 ± 0.228
0.898TyrGln: 0.898 ± 0.185
2.046TyrArg: 2.046 ± 0.34
1.298TyrSer: 1.298 ± 0.253
2.046TyrThr: 2.046 ± 0.353
2.096TyrVal: 2.096 ± 0.301
0.399TyrTrp: 0.399 ± 0.154
0.549TyrTyr: 0.549 ± 0.142
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (20038 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski