Changjiang astro-like virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNS4|A0A1L3KNS4_9VIRU Capsid protein OS=Changjiang astro-like virus OX=1922764 PE=3 SV=1
MM1 pKa = 7.88GGMRR5 pKa = 11.84WQMRR9 pKa = 11.84EE10 pKa = 3.99NTPTLQDD17 pKa = 3.33NTPIWEE23 pKa = 3.96RR24 pKa = 11.84WEE26 pKa = 4.43EE27 pKa = 3.44IDD29 pKa = 5.31YY30 pKa = 10.19PPEE33 pKa = 4.34PEE35 pKa = 3.73WKK37 pKa = 10.13GRR39 pKa = 11.84SLLMEE44 pKa = 4.7DD45 pKa = 3.03VHH47 pKa = 8.77ALEE50 pKa = 4.1NHH52 pKa = 6.05GMPVYY57 pKa = 9.18NTSFSSFTVVANVNINRR74 pKa = 11.84PCSKK78 pKa = 10.3KK79 pKa = 10.6DD80 pKa = 3.05PAPNVEE86 pKa = 4.4CEE88 pKa = 4.07MAVMSVLEE96 pKa = 4.42GYY98 pKa = 9.0RR99 pKa = 11.84AKK101 pKa = 10.68LSEE104 pKa = 4.14YY105 pKa = 10.07QRR107 pKa = 11.84SGWDD111 pKa = 3.23MEE113 pKa = 4.38VLEE116 pKa = 4.2NTFNKK121 pKa = 9.98FKK123 pKa = 9.22THH125 pKa = 5.49VYY127 pKa = 10.19NVNQCAWTAASDD139 pKa = 4.06VMRR142 pKa = 11.84HH143 pKa = 5.46EE144 pKa = 4.05YY145 pKa = 10.31RR146 pKa = 11.84YY147 pKa = 8.76VPRR150 pKa = 11.84RR151 pKa = 11.84IVDD154 pKa = 3.64FHH156 pKa = 6.66EE157 pKa = 4.2IKK159 pKa = 10.84KK160 pKa = 10.2PVDD163 pKa = 3.94KK164 pKa = 11.3SPGWPGINFASTIEE178 pKa = 4.1EE179 pKa = 3.89WRR181 pKa = 11.84EE182 pKa = 3.97LNPHH186 pKa = 6.37RR187 pKa = 11.84AISLWAEE194 pKa = 3.7MSKK197 pKa = 10.71RR198 pKa = 11.84PVFTGAYY205 pKa = 9.9AFIKK209 pKa = 10.38DD210 pKa = 3.72EE211 pKa = 4.77PIKK214 pKa = 10.75VEE216 pKa = 4.17KK217 pKa = 10.5IKK219 pKa = 10.27TKK221 pKa = 8.61DD222 pKa = 3.18QRR224 pKa = 11.84LILCWDD230 pKa = 4.47DD231 pKa = 5.02SFQLCKK237 pKa = 10.62LRR239 pKa = 11.84FQYY242 pKa = 10.61DD243 pKa = 2.78DD244 pKa = 3.4HH245 pKa = 8.22MIMKK249 pKa = 9.34RR250 pKa = 11.84NWMQSEE256 pKa = 4.52SKK258 pKa = 10.05MGWSPLIGGLHH269 pKa = 7.16DD270 pKa = 5.58SIAPLLSYY278 pKa = 10.12RR279 pKa = 11.84YY280 pKa = 9.89KK281 pKa = 10.6LQEE284 pKa = 3.89DD285 pKa = 4.97FKK287 pKa = 11.08RR288 pKa = 11.84FDD290 pKa = 3.35GTICEE295 pKa = 4.07EE296 pKa = 4.13LMLEE300 pKa = 4.48VYY302 pKa = 11.29SMDD305 pKa = 3.43WDD307 pKa = 3.8NLSYY311 pKa = 10.42EE312 pKa = 4.91HH313 pKa = 6.65KK314 pKa = 9.79TEE316 pKa = 4.54DD317 pKa = 3.82NNMRR321 pKa = 11.84YY322 pKa = 10.25YY323 pKa = 10.97NIVHH327 pKa = 6.2NSIHH331 pKa = 4.83THH333 pKa = 6.42EE334 pKa = 4.84ILPNGDD340 pKa = 3.57VVRR343 pKa = 11.84LAHH346 pKa = 6.28GNKK349 pKa = 9.94SGTSDD354 pKa = 3.52TTPLNAKK361 pKa = 9.93VNTFLKK367 pKa = 9.61AYY369 pKa = 9.7EE370 pKa = 3.96MAAFLLSQGEE380 pKa = 4.34SAEE383 pKa = 4.43EE384 pKa = 3.65ILDD387 pKa = 3.74YY388 pKa = 10.53TYY390 pKa = 11.24HH391 pKa = 7.66DD392 pKa = 3.44WRR394 pKa = 11.84QLYY397 pKa = 8.91TMITYY402 pKa = 10.86GDD404 pKa = 3.86DD405 pKa = 3.46RR406 pKa = 11.84LVGEE410 pKa = 4.83DD411 pKa = 4.38LEE413 pKa = 5.38IPLEE417 pKa = 4.14FKK419 pKa = 10.93EE420 pKa = 4.54NIYY423 pKa = 10.8KK424 pKa = 10.05EE425 pKa = 4.02LGMWLPKK432 pKa = 10.64DD433 pKa = 3.4KK434 pKa = 10.55IKK436 pKa = 10.97RR437 pKa = 11.84QDD439 pKa = 3.55YY440 pKa = 10.51VEE442 pKa = 4.45GLSFCGAVIKK452 pKa = 10.44RR453 pKa = 11.84DD454 pKa = 3.69PCTKK458 pKa = 9.81RR459 pKa = 11.84YY460 pKa = 9.22YY461 pKa = 10.2PCFGDD466 pKa = 3.43NDD468 pKa = 3.9KK469 pKa = 11.56LIDD472 pKa = 3.72SFVWKK477 pKa = 10.4NADD480 pKa = 3.76FEE482 pKa = 4.89EE483 pKa = 4.63KK484 pKa = 10.55LLNYY488 pKa = 9.98VLISYY493 pKa = 8.67GGKK496 pKa = 9.38YY497 pKa = 7.67HH498 pKa = 7.26TSFLKK503 pKa = 8.73MAEE506 pKa = 4.18MNGVHH511 pKa = 6.25VPDD514 pKa = 4.46PQDD517 pKa = 3.03IRR519 pKa = 11.84SLIVGWW525 pKa = 3.7

Molecular weight: 61.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNU6|A0A1L3KNU6_9VIRU Uncharacterized protein OS=Changjiang astro-like virus OX=1922764 PE=4 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.65KK4 pKa = 9.98KK5 pKa = 10.27GKK7 pKa = 10.05KK8 pKa = 8.41KK9 pKa = 10.23VVVEE13 pKa = 4.1EE14 pKa = 4.32VVVASSPTTEE24 pKa = 3.64KK25 pKa = 10.51KK26 pKa = 9.77KK27 pKa = 10.43RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84QKK32 pKa = 10.34KK33 pKa = 7.16RR34 pKa = 11.84QKK36 pKa = 9.05RR37 pKa = 11.84TAIADD42 pKa = 3.74AQAKK46 pKa = 7.16PQKK49 pKa = 10.41LPVITAYY56 pKa = 8.63STKK59 pKa = 9.6TVQISTRR66 pKa = 11.84TNKK69 pKa = 10.49NGDD72 pKa = 3.03IVMSGRR78 pKa = 11.84DD79 pKa = 3.28RR80 pKa = 11.84LEE82 pKa = 3.72VVTFPNIVTAEE93 pKa = 4.13TCVLNEE99 pKa = 3.88FLVSPKK105 pKa = 9.69YY106 pKa = 10.9FKK108 pKa = 10.66MNSRR112 pKa = 11.84LSKK115 pKa = 10.59VAANWEE121 pKa = 4.06KK122 pKa = 10.68YY123 pKa = 9.92RR124 pKa = 11.84FVKK127 pKa = 9.35FTVDD131 pKa = 3.37YY132 pKa = 10.05VPSVPSTISGQLIMSFDD149 pKa = 4.61PDD151 pKa = 3.7PEE153 pKa = 4.31GTVAGGDD160 pKa = 3.74YY161 pKa = 9.35QTVAEE166 pKa = 4.43FCMNRR171 pKa = 11.84QRR173 pKa = 11.84SVMFSINEE181 pKa = 3.98HH182 pKa = 6.03KK183 pKa = 10.27RR184 pKa = 11.84VSLNCDD190 pKa = 3.04PKK192 pKa = 11.22LGINDD197 pKa = 3.54WFVDD201 pKa = 3.98LEE203 pKa = 4.77SNGEE207 pKa = 4.04IKK209 pKa = 9.93LTAQGRR215 pKa = 11.84FDD217 pKa = 4.72LVVSTPLISGSQITQSLVVGTLFADD242 pKa = 3.59WEE244 pKa = 4.73VILKK248 pKa = 9.57QPQRR252 pKa = 11.84AQNTGAVTSRR262 pKa = 11.84SLSEE266 pKa = 3.96YY267 pKa = 10.99LNWEE271 pKa = 3.59YY272 pKa = 10.2WAPLLEE278 pKa = 4.48LSADD282 pKa = 3.41VTYY285 pKa = 10.01FNCKK289 pKa = 7.02STYY292 pKa = 10.95AVTNGFKK299 pKa = 10.71FINNSKK305 pKa = 10.2GVCEE309 pKa = 4.33TKK311 pKa = 10.75NVGYY315 pKa = 9.93VANANNGNTKK325 pKa = 10.52AGVISDD331 pKa = 3.46MMAGRR336 pKa = 11.84VYY338 pKa = 10.49VCRR341 pKa = 11.84GSFFPIGEE349 pKa = 3.83QWRR352 pKa = 11.84TYY354 pKa = 6.45TLKK357 pKa = 11.14GITVTALTRR366 pKa = 11.84NTYY369 pKa = 10.08IFFMNNFSTVDD380 pKa = 3.31NSNPLRR386 pKa = 11.84DD387 pKa = 3.7INVDD391 pKa = 3.16TDD393 pKa = 3.53SGCRR397 pKa = 11.84DD398 pKa = 2.77IVMGVRR404 pKa = 11.84FLNTQAGNMQIGYY417 pKa = 8.17YY418 pKa = 9.11TDD420 pKa = 5.07ASIQSDD426 pKa = 4.29SYY428 pKa = 9.34TRR430 pKa = 11.84QVTKK434 pKa = 10.79CGDD437 pKa = 3.48ALQGQILRR445 pKa = 11.84LLARR449 pKa = 11.84ASMWSADD456 pKa = 3.66SEE458 pKa = 4.32EE459 pKa = 4.31APRR462 pKa = 11.84LSKK465 pKa = 9.18TQIPTLEE472 pKa = 4.02DD473 pKa = 2.9RR474 pKa = 11.84LRR476 pKa = 11.84KK477 pKa = 9.34IEE479 pKa = 4.0EE480 pKa = 4.15TLDD483 pKa = 3.66VNTSRR488 pKa = 11.84YY489 pKa = 9.43EE490 pKa = 4.14GEE492 pKa = 4.23FGCC495 pKa = 6.03

Molecular weight: 55.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2102

302

780

525.5

60.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.328 ± 0.34

2.521 ± 0.701

5.947 ± 0.326

5.804 ± 0.791

3.092 ± 0.484

6.518 ± 0.618

2.426 ± 0.569

5.852 ± 0.277

5.899 ± 0.926

8.23 ± 0.554

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.997 ± 0.483

4.757 ± 0.617

4.044 ± 0.305

3.425 ± 0.391

5.804 ± 0.738

6.137 ± 0.497

6.185 ± 0.754

7.945 ± 0.741

2.569 ± 0.415

4.52 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski