Amino acid dipepetide frequency for Rhodoplanes sp. JGI PP 4-B12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.932AlaAla: 17.932 ± 0.785
1.026AlaCys: 1.026 ± 0.146
6.974AlaAsp: 6.974 ± 0.378
7.253AlaGlu: 7.253 ± 0.353
4.226AlaPhe: 4.226 ± 0.296
9.531AlaGly: 9.531 ± 0.511
2.191AlaHis: 2.191 ± 0.185
6.366AlaIle: 6.366 ± 0.357
4.592AlaLys: 4.592 ± 0.318
13.723AlaLeu: 13.723 ± 0.463
3.374AlaMet: 3.374 ± 0.257
3.809AlaAsn: 3.809 ± 0.246
5.879AlaPro: 5.879 ± 0.285
4.609AlaGln: 4.609 ± 0.295
9.183AlaArg: 9.183 ± 0.494
6.244AlaSer: 6.244 ± 0.378
5.861AlaThr: 5.861 ± 0.266
9.061AlaVal: 9.061 ± 0.444
1.6AlaTrp: 1.6 ± 0.188
2.47AlaTyr: 2.47 ± 0.202
0.0AlaXaa: 0.0 ± 0.0
Cys
0.991CysAla: 0.991 ± 0.152
0.087CysCys: 0.087 ± 0.037
0.661CysAsp: 0.661 ± 0.105
0.278CysGlu: 0.278 ± 0.071
0.348CysPhe: 0.348 ± 0.076
1.183CysGly: 1.183 ± 0.163
0.296CysHis: 0.296 ± 0.077
0.452CysIle: 0.452 ± 0.087
0.191CysLys: 0.191 ± 0.054
0.748CysLeu: 0.748 ± 0.113
0.261CysMet: 0.261 ± 0.067
0.296CysAsn: 0.296 ± 0.074
0.504CysPro: 0.504 ± 0.094
0.452CysGln: 0.452 ± 0.08
0.678CysArg: 0.678 ± 0.11
0.626CysSer: 0.626 ± 0.121
0.452CysThr: 0.452 ± 0.094
0.644CysVal: 0.644 ± 0.098
0.122CysTrp: 0.122 ± 0.046
0.209CysTyr: 0.209 ± 0.063
0.0CysXaa: 0.0 ± 0.0
Asp
6.609AspAla: 6.609 ± 0.324
0.522AspCys: 0.522 ± 0.08
3.392AspAsp: 3.392 ± 0.271
3.931AspGlu: 3.931 ± 0.321
2.418AspPhe: 2.418 ± 0.185
4.626AspGly: 4.626 ± 0.306
1.339AspHis: 1.339 ± 0.156
2.644AspIle: 2.644 ± 0.211
1.826AspLys: 1.826 ± 0.199
5.287AspLeu: 5.287 ± 0.369
0.835AspMet: 0.835 ± 0.124
1.531AspAsn: 1.531 ± 0.158
3.809AspPro: 3.809 ± 0.233
1.722AspGln: 1.722 ± 0.18
4.696AspArg: 4.696 ± 0.277
2.331AspSer: 2.331 ± 0.185
2.191AspThr: 2.191 ± 0.198
3.965AspVal: 3.965 ± 0.268
0.939AspTrp: 0.939 ± 0.175
1.913AspTyr: 1.913 ± 0.204
0.0AspXaa: 0.0 ± 0.0
Glu
6.574GluAla: 6.574 ± 0.416
0.435GluCys: 0.435 ± 0.094
2.731GluAsp: 2.731 ± 0.217
2.557GluGlu: 2.557 ± 0.29
2.035GluPhe: 2.035 ± 0.176
3.479GluGly: 3.479 ± 0.251
1.131GluHis: 1.131 ± 0.14
3.322GluIle: 3.322 ± 0.238
2.244GluLys: 2.244 ± 0.225
5.67GluLeu: 5.67 ± 0.318
1.165GluMet: 1.165 ± 0.145
1.67GluAsn: 1.67 ± 0.191
2.852GluPro: 2.852 ± 0.214
2.261GluGln: 2.261 ± 0.221
5.287GluArg: 5.287 ± 0.318
2.383GluSer: 2.383 ± 0.241
2.922GluThr: 2.922 ± 0.264
3.635GluVal: 3.635 ± 0.277
0.991GluTrp: 0.991 ± 0.118
1.044GluTyr: 1.044 ± 0.153
0.0GluXaa: 0.0 ± 0.0
Phe
5.009PheAla: 5.009 ± 0.332
0.452PheCys: 0.452 ± 0.074
2.661PheAsp: 2.661 ± 0.22
2.122PheGlu: 2.122 ± 0.188
1.2PhePhe: 1.2 ± 0.168
3.409PheGly: 3.409 ± 0.291
0.887PheHis: 0.887 ± 0.121
1.531PheIle: 1.531 ± 0.181
1.27PheLys: 1.27 ± 0.137
3.044PheLeu: 3.044 ± 0.252
0.748PheMet: 0.748 ± 0.122
1.113PheAsn: 1.113 ± 0.135
1.809PhePro: 1.809 ± 0.192
0.957PheGln: 0.957 ± 0.166
2.209PheArg: 2.209 ± 0.18
1.983PheSer: 1.983 ± 0.194
1.757PheThr: 1.757 ± 0.157
2.939PheVal: 2.939 ± 0.215
0.435PheTrp: 0.435 ± 0.108
1.235PheTyr: 1.235 ± 0.154
0.0PheXaa: 0.0 ± 0.0
Gly
9.618GlyAla: 9.618 ± 0.491
0.748GlyCys: 0.748 ± 0.133
3.757GlyAsp: 3.757 ± 0.25
4.713GlyGlu: 4.713 ± 0.325
3.479GlyPhe: 3.479 ± 0.238
6.992GlyGly: 6.992 ± 0.471
1.878GlyHis: 1.878 ± 0.179
4.661GlyIle: 4.661 ± 0.297
3.618GlyLys: 3.618 ± 0.282
7.774GlyLeu: 7.774 ± 0.396
2.157GlyMet: 2.157 ± 0.197
2.557GlyAsn: 2.557 ± 0.201
3.496GlyPro: 3.496 ± 0.238
2.696GlyGln: 2.696 ± 0.263
6.0GlyArg: 6.0 ± 0.333
3.965GlySer: 3.965 ± 0.238
4.052GlyThr: 4.052 ± 0.274
5.079GlyVal: 5.079 ± 0.295
1.165GlyTrp: 1.165 ± 0.156
2.278GlyTyr: 2.278 ± 0.185
0.0GlyXaa: 0.0 ± 0.0
His
2.922HisAla: 2.922 ± 0.246
0.348HisCys: 0.348 ± 0.088
1.096HisAsp: 1.096 ± 0.131
1.009HisGlu: 1.009 ± 0.167
0.765HisPhe: 0.765 ± 0.094
1.774HisGly: 1.774 ± 0.163
0.644HisHis: 0.644 ± 0.121
0.835HisIle: 0.835 ± 0.113
0.591HisLys: 0.591 ± 0.096
1.652HisLeu: 1.652 ± 0.176
0.487HisMet: 0.487 ± 0.098
0.504HisAsn: 0.504 ± 0.096
1.687HisPro: 1.687 ± 0.164
0.783HisGln: 0.783 ± 0.089
1.704HisArg: 1.704 ± 0.175
1.026HisSer: 1.026 ± 0.126
0.852HisThr: 0.852 ± 0.134
1.374HisVal: 1.374 ± 0.184
0.452HisTrp: 0.452 ± 0.09
0.574HisTyr: 0.574 ± 0.099
0.0HisXaa: 0.0 ± 0.0
Ile
8.192IleAla: 8.192 ± 0.444
0.435IleCys: 0.435 ± 0.088
4.035IleAsp: 4.035 ± 0.294
3.948IleGlu: 3.948 ± 0.254
1.652IlePhe: 1.652 ± 0.211
5.322IleGly: 5.322 ± 0.323
1.009IleHis: 1.009 ± 0.139
2.278IleIle: 2.278 ± 0.226
1.983IleLys: 1.983 ± 0.205
4.0IleLeu: 4.0 ± 0.259
0.713IleMet: 0.713 ± 0.111
1.096IleAsn: 1.096 ± 0.127
2.331IlePro: 2.331 ± 0.222
1.339IleGln: 1.339 ± 0.192
3.322IleArg: 3.322 ± 0.266
2.365IleSer: 2.365 ± 0.226
2.296IleThr: 2.296 ± 0.196
4.731IleVal: 4.731 ± 0.307
0.678IleTrp: 0.678 ± 0.102
1.287IleTyr: 1.287 ± 0.132
0.0IleXaa: 0.0 ± 0.0
Lys
3.792LysAla: 3.792 ± 0.245
0.174LysCys: 0.174 ± 0.057
1.878LysAsp: 1.878 ± 0.162
1.652LysGlu: 1.652 ± 0.184
1.426LysPhe: 1.426 ± 0.196
2.365LysGly: 2.365 ± 0.227
1.026LysHis: 1.026 ± 0.176
2.018LysIle: 2.018 ± 0.196
1.618LysLys: 1.618 ± 0.211
3.705LysLeu: 3.705 ± 0.286
0.783LysMet: 0.783 ± 0.118
1.148LysAsn: 1.148 ± 0.157
2.574LysPro: 2.574 ± 0.249
1.113LysGln: 1.113 ± 0.147
2.905LysArg: 2.905 ± 0.288
2.522LysSer: 2.522 ± 0.228
2.331LysThr: 2.331 ± 0.236
2.539LysVal: 2.539 ± 0.219
0.504LysTrp: 0.504 ± 0.091
0.748LysTyr: 0.748 ± 0.126
0.0LysXaa: 0.0 ± 0.0
Leu
13.097LeuAla: 13.097 ± 0.528
1.165LeuCys: 1.165 ± 0.139
5.931LeuAsp: 5.931 ± 0.331
4.383LeuGlu: 4.383 ± 0.305
3.6LeuPhe: 3.6 ± 0.307
7.861LeuGly: 7.861 ± 0.364
1.931LeuHis: 1.931 ± 0.185
5.409LeuIle: 5.409 ± 0.322
3.844LeuLys: 3.844 ± 0.288
8.853LeuLeu: 8.853 ± 0.47
2.087LeuMet: 2.087 ± 0.181
2.383LeuAsn: 2.383 ± 0.208
4.87LeuPro: 4.87 ± 0.282
2.696LeuGln: 2.696 ± 0.23
7.079LeuArg: 7.079 ± 0.343
5.061LeuSer: 5.061 ± 0.326
5.079LeuThr: 5.079 ± 0.292
6.887LeuVal: 6.887 ± 0.415
1.461LeuTrp: 1.461 ± 0.181
2.052LeuTyr: 2.052 ± 0.193
0.0LeuXaa: 0.0 ± 0.0
Met
2.748MetAla: 2.748 ± 0.204
0.122MetCys: 0.122 ± 0.05
0.974MetAsp: 0.974 ± 0.139
0.817MetGlu: 0.817 ± 0.111
0.591MetPhe: 0.591 ± 0.096
1.496MetGly: 1.496 ± 0.16
0.47MetHis: 0.47 ± 0.104
1.478MetIle: 1.478 ± 0.129
0.887MetLys: 0.887 ± 0.13
2.07MetLeu: 2.07 ± 0.179
0.817MetMet: 0.817 ± 0.118
0.765MetAsn: 0.765 ± 0.12
1.618MetPro: 1.618 ± 0.172
0.974MetGln: 0.974 ± 0.117
1.739MetArg: 1.739 ± 0.142
1.426MetSer: 1.426 ± 0.151
1.583MetThr: 1.583 ± 0.148
1.513MetVal: 1.513 ± 0.186
0.33MetTrp: 0.33 ± 0.081
0.33MetTyr: 0.33 ± 0.065
0.0MetXaa: 0.0 ± 0.0
Asn
3.357AsnAla: 3.357 ± 0.243
0.278AsnCys: 0.278 ± 0.074
1.548AsnAsp: 1.548 ± 0.135
1.339AsnGlu: 1.339 ± 0.162
0.8AsnPhe: 0.8 ± 0.119
2.661AsnGly: 2.661 ± 0.195
0.591AsnHis: 0.591 ± 0.105
1.739AsnIle: 1.739 ± 0.182
0.87AsnLys: 0.87 ± 0.128
2.574AsnLeu: 2.574 ± 0.206
0.487AsnMet: 0.487 ± 0.086
0.678AsnAsn: 0.678 ± 0.122
2.209AsnPro: 2.209 ± 0.213
0.835AsnGln: 0.835 ± 0.151
2.191AsnArg: 2.191 ± 0.217
1.583AsnSer: 1.583 ± 0.181
1.27AsnThr: 1.27 ± 0.159
1.844AsnVal: 1.844 ± 0.18
0.609AsnTrp: 0.609 ± 0.105
1.009AsnTyr: 1.009 ± 0.126
0.0AsnXaa: 0.0 ± 0.0
Pro
6.957ProAla: 6.957 ± 0.37
0.33ProCys: 0.33 ± 0.071
4.087ProAsp: 4.087 ± 0.272
3.426ProGlu: 3.426 ± 0.252
1.983ProPhe: 1.983 ± 0.183
4.592ProGly: 4.592 ± 0.281
1.27ProHis: 1.27 ± 0.154
2.922ProIle: 2.922 ± 0.242
2.296ProLys: 2.296 ± 0.217
4.574ProLeu: 4.574 ± 0.308
1.183ProMet: 1.183 ± 0.14
1.565ProAsn: 1.565 ± 0.189
3.652ProPro: 3.652 ± 0.342
2.331ProGln: 2.331 ± 0.211
3.948ProArg: 3.948 ± 0.252
3.009ProSer: 3.009 ± 0.256
2.226ProThr: 2.226 ± 0.24
3.774ProVal: 3.774 ± 0.307
0.748ProTrp: 0.748 ± 0.109
1.652ProTyr: 1.652 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
3.983GlnAla: 3.983 ± 0.259
0.296GlnCys: 0.296 ± 0.077
1.461GlnAsp: 1.461 ± 0.164
1.27GlnGlu: 1.27 ± 0.149
1.165GlnPhe: 1.165 ± 0.141
2.418GlnGly: 2.418 ± 0.252
0.87GlnHis: 0.87 ± 0.13
2.244GlnIle: 2.244 ± 0.189
1.357GlnLys: 1.357 ± 0.15
3.078GlnLeu: 3.078 ± 0.247
0.835GlnMet: 0.835 ± 0.139
1.2GlnAsn: 1.2 ± 0.151
2.122GlnPro: 2.122 ± 0.169
1.722GlnGln: 1.722 ± 0.194
2.505GlnArg: 2.505 ± 0.21
2.0GlnSer: 2.0 ± 0.201
2.104GlnThr: 2.104 ± 0.181
2.018GlnVal: 2.018 ± 0.215
0.383GlnTrp: 0.383 ± 0.08
0.974GlnTyr: 0.974 ± 0.13
0.0GlnXaa: 0.0 ± 0.0
Arg
8.783ArgAla: 8.783 ± 0.419
0.661ArgCys: 0.661 ± 0.126
4.105ArgAsp: 4.105 ± 0.295
4.574ArgGlu: 4.574 ± 0.333
2.8ArgPhe: 2.8 ± 0.23
5.409ArgGly: 5.409 ± 0.293
1.531ArgHis: 1.531 ± 0.167
4.122ArgIle: 4.122 ± 0.244
2.626ArgLys: 2.626 ± 0.241
8.035ArgLeu: 8.035 ± 0.331
2.035ArgMet: 2.035 ± 0.17
2.261ArgAsn: 2.261 ± 0.199
4.105ArgPro: 4.105 ± 0.277
2.765ArgGln: 2.765 ± 0.227
6.209ArgArg: 6.209 ± 0.39
3.635ArgSer: 3.635 ± 0.253
3.6ArgThr: 3.6 ± 0.244
4.783ArgVal: 4.783 ± 0.278
0.957ArgTrp: 0.957 ± 0.12
1.809ArgTyr: 1.809 ± 0.191
0.0ArgXaa: 0.0 ± 0.0
Ser
6.261SerAla: 6.261 ± 0.417
0.678SerCys: 0.678 ± 0.104
3.078SerAsp: 3.078 ± 0.202
2.47SerGlu: 2.47 ± 0.18
2.104SerPhe: 2.104 ± 0.21
4.783SerGly: 4.783 ± 0.301
0.87SerHis: 0.87 ± 0.135
2.557SerIle: 2.557 ± 0.203
1.948SerLys: 1.948 ± 0.221
4.505SerLeu: 4.505 ± 0.319
1.235SerMet: 1.235 ± 0.144
1.565SerAsn: 1.565 ± 0.174
2.957SerPro: 2.957 ± 0.253
1.513SerGln: 1.513 ± 0.171
3.844SerArg: 3.844 ± 0.251
3.009SerSer: 3.009 ± 0.225
2.939SerThr: 2.939 ± 0.263
3.409SerVal: 3.409 ± 0.241
0.504SerTrp: 0.504 ± 0.11
1.531SerTyr: 1.531 ± 0.159
0.0SerXaa: 0.0 ± 0.0
Thr
6.8ThrAla: 6.8 ± 0.315
0.417ThrCys: 0.417 ± 0.087
2.365ThrAsp: 2.365 ± 0.194
2.505ThrGlu: 2.505 ± 0.228
1.896ThrPhe: 1.896 ± 0.191
4.939ThrGly: 4.939 ± 0.329
1.026ThrHis: 1.026 ± 0.16
3.078ThrIle: 3.078 ± 0.233
1.548ThrLys: 1.548 ± 0.159
4.905ThrLeu: 4.905 ± 0.307
1.148ThrMet: 1.148 ± 0.127
1.252ThrAsn: 1.252 ± 0.149
3.479ThrPro: 3.479 ± 0.256
1.235ThrGln: 1.235 ± 0.162
3.252ThrArg: 3.252 ± 0.231
2.748ThrSer: 2.748 ± 0.22
2.905ThrThr: 2.905 ± 0.231
4.07ThrVal: 4.07 ± 0.327
0.678ThrTrp: 0.678 ± 0.099
1.096ThrTyr: 1.096 ± 0.129
0.0ThrXaa: 0.0 ± 0.0
Val
8.435ValAla: 8.435 ± 0.382
0.713ValCys: 0.713 ± 0.121
3.774ValAsp: 3.774 ± 0.231
4.47ValGlu: 4.47 ± 0.254
2.591ValPhe: 2.591 ± 0.254
4.835ValGly: 4.835 ± 0.298
1.513ValHis: 1.513 ± 0.138
3.705ValIle: 3.705 ± 0.257
2.383ValLys: 2.383 ± 0.16
7.357ValLeu: 7.357 ± 0.38
1.478ValMet: 1.478 ± 0.163
1.931ValAsn: 1.931 ± 0.188
4.418ValPro: 4.418 ± 0.303
2.365ValGln: 2.365 ± 0.201
4.748ValArg: 4.748 ± 0.332
3.392ValSer: 3.392 ± 0.252
4.644ValThr: 4.644 ± 0.299
6.105ValVal: 6.105 ± 0.401
0.887ValTrp: 0.887 ± 0.139
1.531ValTyr: 1.531 ± 0.163
0.0ValXaa: 0.0 ± 0.0
Trp
0.991TrpAla: 0.991 ± 0.126
0.226TrpCys: 0.226 ± 0.053
0.609TrpAsp: 0.609 ± 0.123
0.557TrpGlu: 0.557 ± 0.088
0.487TrpPhe: 0.487 ± 0.092
0.783TrpGly: 0.783 ± 0.116
0.313TrpHis: 0.313 ± 0.088
0.748TrpIle: 0.748 ± 0.121
0.47TrpLys: 0.47 ± 0.112
1.583TrpLeu: 1.583 ± 0.188
0.417TrpMet: 0.417 ± 0.099
0.365TrpAsn: 0.365 ± 0.072
0.817TrpPro: 0.817 ± 0.119
0.661TrpGln: 0.661 ± 0.092
1.287TrpArg: 1.287 ± 0.168
1.044TrpSer: 1.044 ± 0.145
0.922TrpThr: 0.922 ± 0.12
0.991TrpVal: 0.991 ± 0.145
0.157TrpTrp: 0.157 ± 0.048
0.313TrpTyr: 0.313 ± 0.066
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.87TyrAla: 2.87 ± 0.202
0.365TyrCys: 0.365 ± 0.079
1.409TyrAsp: 1.409 ± 0.154
1.113TyrGlu: 1.113 ± 0.146
1.2TyrPhe: 1.2 ± 0.16
2.226TyrGly: 2.226 ± 0.201
0.296TyrHis: 0.296 ± 0.071
0.904TyrIle: 0.904 ± 0.129
0.661TyrLys: 0.661 ± 0.095
2.591TyrLeu: 2.591 ± 0.208
0.487TyrMet: 0.487 ± 0.097
0.783TyrAsn: 0.783 ± 0.129
1.339TyrPro: 1.339 ± 0.177
0.974TyrGln: 0.974 ± 0.111
2.018TyrArg: 2.018 ± 0.198
1.409TyrSer: 1.409 ± 0.15
1.357TyrThr: 1.357 ± 0.167
1.913TyrVal: 1.913 ± 0.163
0.139TyrTrp: 0.139 ± 0.053
0.887TyrTyr: 0.887 ± 0.129
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 220 proteins (57497 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski