Amino acid dipepetide frequency for Bacillus phage vB_BanS-Tsamsa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.0AlaAla: 0.0 ± 0.0
0.578AlaCys: 0.578 ± 0.101
3.331AlaAsp: 3.331 ± 0.307
4.528AlaGlu: 4.528 ± 0.331
2.433AlaPhe: 2.433 ± 0.193
3.052AlaGly: 3.052 ± 0.355
1.117AlaHis: 1.117 ± 0.149
4.787AlaIle: 4.787 ± 0.302
5.286AlaLys: 5.286 ± 0.572
4.548AlaLeu: 4.548 ± 0.276
1.975AlaMet: 1.975 ± 0.264
3.191AlaAsn: 3.191 ± 0.311
1.596AlaPro: 1.596 ± 0.189
1.775AlaGln: 1.775 ± 0.356
2.254AlaArg: 2.254 ± 0.219
3.391AlaSer: 3.391 ± 0.461
4.049AlaThr: 4.049 ± 0.451
3.85AlaVal: 3.85 ± 0.301
0.558AlaTrp: 0.558 ± 0.094
2.453AlaTyr: 2.453 ± 0.203
0.0AlaXaa: 0.0 ± 0.0
Cys
0.439CysAla: 0.439 ± 0.094
0.08CysCys: 0.08 ± 0.037
0.558CysAsp: 0.558 ± 0.132
0.638CysGlu: 0.638 ± 0.139
0.259CysPhe: 0.259 ± 0.074
0.778CysGly: 0.778 ± 0.147
0.219CysHis: 0.219 ± 0.064
0.618CysIle: 0.618 ± 0.104
0.678CysLys: 0.678 ± 0.119
0.339CysLeu: 0.339 ± 0.08
0.499CysMet: 0.499 ± 0.119
0.479CysAsn: 0.479 ± 0.118
0.319CysPro: 0.319 ± 0.088
0.319CysGln: 0.319 ± 0.09
0.439CysArg: 0.439 ± 0.086
0.359CysSer: 0.359 ± 0.079
0.399CysThr: 0.399 ± 0.099
0.718CysVal: 0.718 ± 0.117
0.14CysTrp: 0.14 ± 0.054
0.539CysTyr: 0.539 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
3.291AspAla: 3.291 ± 0.296
0.838AspCys: 0.838 ± 0.148
4.328AspAsp: 4.328 ± 0.395
5.864AspGlu: 5.864 ± 0.388
3.511AspPhe: 3.511 ± 0.257
5.505AspGly: 5.505 ± 0.307
0.618AspHis: 0.618 ± 0.14
4.727AspIle: 4.727 ± 0.37
5.884AspLys: 5.884 ± 0.453
5.605AspLeu: 5.605 ± 0.364
2.094AspMet: 2.094 ± 0.22
3.391AspAsn: 3.391 ± 0.276
0.818AspPro: 0.818 ± 0.149
1.057AspGln: 1.057 ± 0.19
2.374AspArg: 2.374 ± 0.223
3.451AspSer: 3.451 ± 0.275
3.65AspThr: 3.65 ± 0.281
4.129AspVal: 4.129 ± 0.264
0.997AspTrp: 0.997 ± 0.162
3.251AspTyr: 3.251 ± 0.261
0.0AspXaa: 0.0 ± 0.0
Glu
4.488GluAla: 4.488 ± 0.467
0.499GluCys: 0.499 ± 0.116
5.485GluAsp: 5.485 ± 0.478
6.782GluGlu: 6.782 ± 0.443
3.471GluPhe: 3.471 ± 0.294
4.408GluGly: 4.408 ± 0.3
1.416GluHis: 1.416 ± 0.173
6.822GluIle: 6.822 ± 0.399
7.121GluLys: 7.121 ± 0.493
7.639GluLeu: 7.639 ± 0.423
2.912GluMet: 2.912 ± 0.25
4.508GluAsn: 4.508 ± 0.267
1.456GluPro: 1.456 ± 0.177
3.271GluGln: 3.271 ± 0.297
3.351GluArg: 3.351 ± 0.247
3.969GluSer: 3.969 ± 0.306
3.969GluThr: 3.969 ± 0.312
5.565GluVal: 5.565 ± 0.407
0.937GluTrp: 0.937 ± 0.144
3.471GluTyr: 3.471 ± 0.273
0.0GluXaa: 0.0 ± 0.0
Phe
2.194PheAla: 2.194 ± 0.241
0.299PheCys: 0.299 ± 0.073
3.132PheAsp: 3.132 ± 0.287
3.71PheGlu: 3.71 ± 0.326
1.915PhePhe: 1.915 ± 0.242
3.092PheGly: 3.092 ± 0.317
0.918PheHis: 0.918 ± 0.13
3.012PheIle: 3.012 ± 0.302
3.79PheLys: 3.79 ± 0.24
3.371PheLeu: 3.371 ± 0.273
1.277PheMet: 1.277 ± 0.155
2.194PheAsn: 2.194 ± 0.206
0.977PhePro: 0.977 ± 0.138
1.277PheGln: 1.277 ± 0.171
1.895PheArg: 1.895 ± 0.227
2.553PheSer: 2.553 ± 0.236
2.753PheThr: 2.753 ± 0.296
2.493PheVal: 2.493 ± 0.239
0.618PheTrp: 0.618 ± 0.14
1.775PheTyr: 1.775 ± 0.22
0.0PheXaa: 0.0 ± 0.0
Gly
3.67GlyAla: 3.67 ± 0.471
0.698GlyCys: 0.698 ± 0.129
4.029GlyAsp: 4.029 ± 0.275
4.288GlyGlu: 4.288 ± 0.279
3.151GlyPhe: 3.151 ± 0.236
4.169GlyGly: 4.169 ± 0.419
1.237GlyHis: 1.237 ± 0.153
4.827GlyIle: 4.827 ± 0.327
5.904GlyLys: 5.904 ± 0.31
5.425GlyLeu: 5.425 ± 0.304
2.054GlyMet: 2.054 ± 0.233
3.889GlyAsn: 3.889 ± 0.372
0.0GlyPro: 0.0 ± 0.0
1.975GlyGln: 1.975 ± 0.211
2.593GlyArg: 2.593 ± 0.209
3.511GlySer: 3.511 ± 0.375
4.189GlyThr: 4.189 ± 0.406
4.149GlyVal: 4.149 ± 0.324
0.878GlyTrp: 0.878 ± 0.161
3.371GlyTyr: 3.371 ± 0.246
0.0GlyXaa: 0.0 ± 0.0
His
0.858HisAla: 0.858 ± 0.135
0.199HisCys: 0.199 ± 0.066
1.476HisAsp: 1.476 ± 0.182
1.576HisGlu: 1.576 ± 0.201
0.598HisPhe: 0.598 ± 0.123
0.918HisGly: 0.918 ± 0.112
0.519HisHis: 0.519 ± 0.098
1.516HisIle: 1.516 ± 0.195
1.675HisLys: 1.675 ± 0.197
1.396HisLeu: 1.396 ± 0.189
0.459HisMet: 0.459 ± 0.104
1.077HisAsn: 1.077 ± 0.167
0.638HisPro: 0.638 ± 0.135
0.539HisGln: 0.539 ± 0.093
0.778HisArg: 0.778 ± 0.137
1.217HisSer: 1.217 ± 0.192
1.097HisThr: 1.097 ± 0.149
1.576HisVal: 1.576 ± 0.219
0.1HisTrp: 0.1 ± 0.051
0.618HisTyr: 0.618 ± 0.114
0.0HisXaa: 0.0 ± 0.0
Ile
4.249IleAla: 4.249 ± 0.371
0.539IleCys: 0.539 ± 0.118
5.326IleAsp: 5.326 ± 0.316
6.502IleGlu: 6.502 ± 0.433
2.094IlePhe: 2.094 ± 0.213
3.71IleGly: 3.71 ± 0.291
1.476IleHis: 1.476 ± 0.188
4.388IleIle: 4.388 ± 0.364
6.403IleLys: 6.403 ± 0.289
5.106IleLeu: 5.106 ± 0.334
2.114IleMet: 2.114 ± 0.202
3.969IleAsn: 3.969 ± 0.294
1.935IlePro: 1.935 ± 0.204
2.294IleGln: 2.294 ± 0.176
2.633IleArg: 2.633 ± 0.229
3.85IleSer: 3.85 ± 0.231
4.468IleThr: 4.468 ± 0.335
4.967IleVal: 4.967 ± 0.391
0.638IleTrp: 0.638 ± 0.101
3.012IleTyr: 3.012 ± 0.296
0.0IleXaa: 0.0 ± 0.0
Lys
5.864LysAla: 5.864 ± 0.436
0.957LysCys: 0.957 ± 0.185
5.346LysAsp: 5.346 ± 0.359
8.158LysGlu: 8.158 ± 0.462
3.79LysPhe: 3.79 ± 0.276
5.006LysGly: 5.006 ± 0.303
1.795LysHis: 1.795 ± 0.178
5.725LysIle: 5.725 ± 0.381
8.278LysLys: 8.278 ± 0.643
6.941LysLeu: 6.941 ± 0.425
3.371LysMet: 3.371 ± 0.258
4.288LysAsn: 4.288 ± 0.281
2.493LysPro: 2.493 ± 0.287
3.57LysGln: 3.57 ± 0.261
3.909LysArg: 3.909 ± 0.347
4.408LysSer: 4.408 ± 0.345
4.807LysThr: 4.807 ± 0.275
6.363LysVal: 6.363 ± 0.35
1.057LysTrp: 1.057 ± 0.171
4.049LysTyr: 4.049 ± 0.224
0.0LysXaa: 0.0 ± 0.0
Leu
4.727LeuAla: 4.727 ± 0.339
0.578LeuCys: 0.578 ± 0.127
5.545LeuAsp: 5.545 ± 0.362
6.702LeuGlu: 6.702 ± 0.356
3.132LeuPhe: 3.132 ± 0.276
5.046LeuGly: 5.046 ± 0.279
1.616LeuHis: 1.616 ± 0.147
4.628LeuIle: 4.628 ± 0.374
7.3LeuLys: 7.3 ± 0.342
6.642LeuLeu: 6.642 ± 0.54
2.374LeuMet: 2.374 ± 0.266
4.468LeuAsn: 4.468 ± 0.288
1.935LeuPro: 1.935 ± 0.203
2.952LeuGln: 2.952 ± 0.278
3.491LeuArg: 3.491 ± 0.304
4.328LeuSer: 4.328 ± 0.272
5.106LeuThr: 5.106 ± 0.301
4.747LeuVal: 4.747 ± 0.295
0.997LeuTrp: 0.997 ± 0.128
3.231LeuTyr: 3.231 ± 0.291
0.0LeuXaa: 0.0 ± 0.0
Met
2.254MetAla: 2.254 ± 0.303
0.279MetCys: 0.279 ± 0.089
1.476MetAsp: 1.476 ± 0.189
2.334MetGlu: 2.334 ± 0.195
1.077MetPhe: 1.077 ± 0.166
1.915MetGly: 1.915 ± 0.218
0.459MetHis: 0.459 ± 0.087
2.234MetIle: 2.234 ± 0.209
3.73MetLys: 3.73 ± 0.334
2.274MetLeu: 2.274 ± 0.219
1.257MetMet: 1.257 ± 0.159
2.234MetAsn: 2.234 ± 0.203
0.858MetPro: 0.858 ± 0.112
1.217MetGln: 1.217 ± 0.197
1.656MetArg: 1.656 ± 0.163
2.194MetSer: 2.194 ± 0.216
2.015MetThr: 2.015 ± 0.215
1.795MetVal: 1.795 ± 0.157
0.219MetTrp: 0.219 ± 0.074
1.117MetTyr: 1.117 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.231AsnAla: 3.231 ± 0.342
0.359AsnCys: 0.359 ± 0.087
3.271AsnAsp: 3.271 ± 0.308
3.87AsnGlu: 3.87 ± 0.288
2.035AsnPhe: 2.035 ± 0.19
4.767AsnGly: 4.767 ± 0.359
1.017AsnHis: 1.017 ± 0.144
4.249AsnIle: 4.249 ± 0.254
5.206AsnLys: 5.206 ± 0.355
4.268AsnLeu: 4.268 ± 0.274
1.895AsnMet: 1.895 ± 0.261
2.872AsnAsn: 2.872 ± 0.262
1.616AsnPro: 1.616 ± 0.214
1.835AsnGln: 1.835 ± 0.198
2.453AsnArg: 2.453 ± 0.217
2.972AsnSer: 2.972 ± 0.257
2.852AsnThr: 2.852 ± 0.226
3.65AsnVal: 3.65 ± 0.258
0.638AsnTrp: 0.638 ± 0.103
2.733AsnTyr: 2.733 ± 0.304
0.0AsnXaa: 0.0 ± 0.0
Pro
1.257ProAla: 1.257 ± 0.19
0.199ProCys: 0.199 ± 0.062
1.476ProAsp: 1.476 ± 0.137
2.154ProGlu: 2.154 ± 0.252
1.057ProPhe: 1.057 ± 0.145
0.0ProGly: 0.0 ± 0.0
0.638ProHis: 0.638 ± 0.126
1.456ProIle: 1.456 ± 0.19
1.855ProLys: 1.855 ± 0.192
1.596ProLeu: 1.596 ± 0.207
0.419ProMet: 0.419 ± 0.105
1.456ProAsn: 1.456 ± 0.169
0.539ProPro: 0.539 ± 0.144
1.177ProGln: 1.177 ± 0.141
0.718ProArg: 0.718 ± 0.113
1.755ProSer: 1.755 ± 0.201
1.695ProThr: 1.695 ± 0.178
1.935ProVal: 1.935 ± 0.183
0.1ProTrp: 0.1 ± 0.048
0.997ProTyr: 0.997 ± 0.149
0.0ProXaa: 0.0 ± 0.0
Gln
2.433GlnAla: 2.433 ± 0.398
0.16GlnCys: 0.16 ± 0.061
1.656GlnAsp: 1.656 ± 0.166
3.151GlnGlu: 3.151 ± 0.233
1.456GlnPhe: 1.456 ± 0.157
1.955GlnGly: 1.955 ± 0.259
0.439GlnHis: 0.439 ± 0.105
2.254GlnIle: 2.254 ± 0.22
2.952GlnLys: 2.952 ± 0.224
3.271GlnLeu: 3.271 ± 0.327
1.316GlnMet: 1.316 ± 0.212
1.815GlnAsn: 1.815 ± 0.229
0.798GlnPro: 0.798 ± 0.147
1.277GlnGln: 1.277 ± 0.324
1.536GlnArg: 1.536 ± 0.179
1.656GlnSer: 1.656 ± 0.258
1.675GlnThr: 1.675 ± 0.197
2.354GlnVal: 2.354 ± 0.204
0.479GlnTrp: 0.479 ± 0.111
1.316GlnTyr: 1.316 ± 0.158
0.0GlnXaa: 0.0 ± 0.0
Arg
2.094ArgAla: 2.094 ± 0.213
0.359ArgCys: 0.359 ± 0.075
2.673ArgAsp: 2.673 ± 0.295
3.291ArgGlu: 3.291 ± 0.278
2.054ArgPhe: 2.054 ± 0.193
2.553ArgGly: 2.553 ± 0.202
0.778ArgHis: 0.778 ± 0.13
2.294ArgIle: 2.294 ± 0.201
4.029ArgLys: 4.029 ± 0.265
3.132ArgLeu: 3.132 ± 0.295
1.755ArgMet: 1.755 ± 0.204
2.792ArgAsn: 2.792 ± 0.232
1.097ArgPro: 1.097 ± 0.165
1.636ArgGln: 1.636 ± 0.194
1.815ArgArg: 1.815 ± 0.207
1.296ArgSer: 1.296 ± 0.155
2.035ArgThr: 2.035 ± 0.208
2.553ArgVal: 2.553 ± 0.265
0.778ArgTrp: 0.778 ± 0.129
2.094ArgTyr: 2.094 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
3.351SerAla: 3.351 ± 0.531
0.638SerCys: 0.638 ± 0.129
3.231SerAsp: 3.231 ± 0.264
3.69SerGlu: 3.69 ± 0.273
2.653SerPhe: 2.653 ± 0.223
4.169SerGly: 4.169 ± 0.485
1.057SerHis: 1.057 ± 0.139
3.75SerIle: 3.75 ± 0.264
4.368SerLys: 4.368 ± 0.296
4.109SerLeu: 4.109 ± 0.281
1.496SerMet: 1.496 ± 0.212
2.792SerAsn: 2.792 ± 0.312
1.396SerPro: 1.396 ± 0.193
2.015SerGln: 2.015 ± 0.324
2.114SerArg: 2.114 ± 0.208
3.431SerSer: 3.431 ± 0.382
3.75SerThr: 3.75 ± 0.353
3.311SerVal: 3.311 ± 0.256
0.598SerTrp: 0.598 ± 0.105
2.394SerTyr: 2.394 ± 0.27
0.0SerXaa: 0.0 ± 0.0
Thr
3.311ThrAla: 3.311 ± 0.333
0.479ThrCys: 0.479 ± 0.111
3.391ThrAsp: 3.391 ± 0.3
3.77ThrGlu: 3.77 ± 0.275
3.151ThrPhe: 3.151 ± 0.264
4.608ThrGly: 4.608 ± 0.391
1.017ThrHis: 1.017 ± 0.169
4.468ThrIle: 4.468 ± 0.365
5.006ThrLys: 5.006 ± 0.307
5.266ThrLeu: 5.266 ± 0.41
1.596ThrMet: 1.596 ± 0.212
3.271ThrAsn: 3.271 ± 0.296
1.735ThrPro: 1.735 ± 0.239
1.875ThrGln: 1.875 ± 0.204
2.374ThrArg: 2.374 ± 0.217
2.832ThrSer: 2.832 ± 0.281
3.889ThrThr: 3.889 ± 0.432
4.428ThrVal: 4.428 ± 0.374
0.578ThrTrp: 0.578 ± 0.111
3.012ThrTyr: 3.012 ± 0.318
0.0ThrXaa: 0.0 ± 0.0
Val
4.229ValAla: 4.229 ± 0.275
0.439ValCys: 0.439 ± 0.118
5.226ValAsp: 5.226 ± 0.325
6.283ValGlu: 6.283 ± 0.403
3.052ValPhe: 3.052 ± 0.229
4.288ValGly: 4.288 ± 0.316
1.157ValHis: 1.157 ± 0.173
4.049ValIle: 4.049 ± 0.294
5.944ValLys: 5.944 ± 0.323
4.667ValLeu: 4.667 ± 0.358
2.214ValMet: 2.214 ± 0.203
3.67ValAsn: 3.67 ± 0.277
1.097ValPro: 1.097 ± 0.163
1.875ValGln: 1.875 ± 0.24
2.513ValArg: 2.513 ± 0.198
3.63ValSer: 3.63 ± 0.306
4.109ValThr: 4.109 ± 0.346
5.166ValVal: 5.166 ± 0.338
0.818ValTrp: 0.818 ± 0.119
2.633ValTyr: 2.633 ± 0.239
0.0ValXaa: 0.0 ± 0.0
Trp
0.598TrpAla: 0.598 ± 0.097
0.219TrpCys: 0.219 ± 0.067
0.997TrpAsp: 0.997 ± 0.15
0.918TrpGlu: 0.918 ± 0.153
0.399TrpPhe: 0.399 ± 0.092
0.618TrpGly: 0.618 ± 0.137
0.279TrpHis: 0.279 ± 0.078
0.878TrpIle: 0.878 ± 0.133
1.017TrpLys: 1.017 ± 0.147
0.878TrpLeu: 0.878 ± 0.151
0.479TrpMet: 0.479 ± 0.09
0.638TrpAsn: 0.638 ± 0.109
0.0TrpPro: 0.0 ± 0.0
0.419TrpGln: 0.419 ± 0.085
0.479TrpArg: 0.479 ± 0.114
0.838TrpSer: 0.838 ± 0.175
0.638TrpThr: 0.638 ± 0.13
0.678TrpVal: 0.678 ± 0.138
0.199TrpTrp: 0.199 ± 0.076
0.678TrpTyr: 0.678 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.214TyrAla: 2.214 ± 0.204
0.419TyrCys: 0.419 ± 0.104
3.451TyrAsp: 3.451 ± 0.264
3.331TyrGlu: 3.331 ± 0.266
1.955TyrPhe: 1.955 ± 0.242
3.451TyrGly: 3.451 ± 0.329
1.057TyrHis: 1.057 ± 0.16
2.992TyrIle: 2.992 ± 0.27
3.75TyrLys: 3.75 ± 0.278
3.072TyrLeu: 3.072 ± 0.245
1.017TyrMet: 1.017 ± 0.131
2.792TyrAsn: 2.792 ± 0.294
1.177TyrPro: 1.177 ± 0.213
1.596TyrGln: 1.596 ± 0.168
1.735TyrArg: 1.735 ± 0.208
2.673TyrSer: 2.673 ± 0.216
2.912TyrThr: 2.912 ± 0.272
2.633TyrVal: 2.633 ± 0.226
0.519TyrTrp: 0.519 ± 0.111
2.094TyrTyr: 2.094 ± 0.276
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 272 proteins (50136 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski